QC validation of the analytical method ( Absorbance & Concentration). LOD; LOQ; SD
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- Опубліковано 9 лют 2025
- QC validation of the analytical method ( Absorbance & Concentration)
Limit of Detection
Limit of Quantitation
Standard Deviation
#absorbance #concentration #limitofdetection #limitofquantitation #standarddeviation
Gap is obs please make appropriate and details videos.
Hello, how do you calculate the accuracy when the first dilution for your standard curve is 0 ng/mL? Do you just omit this point when calculating standard deviation for percent recoveries since you can't divide by zero and get the percent recovery for the starting 0 ng/mL standard?
Thank you so much.
Nice 😊
Excuse me sir, i understand that you have individuate the method's LOD and LOQ, but in concentration therms or absorbance?
hi, i don' understand how your LOD value can be higher than your first calibration point at 4.4ppm.
He/she must have just picked random values for the standard curve. Sometimes when you are qualifying a method you have to readjust your standard curve to allow you to have the most optimal LOQ. Some points on the lower end just can't be used sometimes. This is just an example. If you developed a standard curve where you are not able to use your first concentration, then you would optimize your method. Alternatively, you would only consider measured sample concentrations above the second point of your standard curve as being reportable. At least I have seen it play out like this before.
Thank you👍
Recovery should come near to 100%
Pls some one explain to me
HELLO what can we do when 3 replicate of response we have?
Take the average of the response for your 3 replicates.
i have too pic in chromatogramme . i do validation method R=0.999 but when i calculat Found concentration is too far to real concentration . can y help me ; i repeat validation mor than 4
how to get this add-ins for excel? I don' have data analysis buttom
go to files, options, add-ons, select analytical tools, run all
conc. in (ppm) ?? how it is taken??.Please explain it. please
2.51 minute ur values show axis absorbance in x axis but u entered concentration in name.is it right.can u explain please.why u change again.which should come in regression.correlataion and regression both can same x axis or vice versa
In the table first must be concentration, then absorbance. I wrote like this, that is way, no need to mention first or second tables. Main thing absorbance must be y, concentration x. Because after known concentration we are finding absorbance. If you will put absorbance x, concentration y, regression will be different.
ua-cam.com/video/gTAk8hL9D-A/v-deo.html
Will you please provide the excel file
Уважаемый автор, укажите формулы расчёта LOD и LOQ для подсчёта вручную
I WANT TO KNOW
HOW THE LOD AND LOQ CALCULATION FOR THE VALUES OF PH
CAN YOU HELP ME ?
It is not for the pH meter. Because everybody knows that the pH level from 1 to 14.
the measurement interval that the laboratory will be accredited is 4-10 . I cant calculate LOD and LOQ . what can i write for these ph parameters? .because I did not count when I sent the validation report, they said us that we have to calculate LOD AND LOQ. THANK YOU
Analytes which are not regulated under Ch. NR 149, including temperature, pH, nutrients in soil and sludge,
physical properties of soil and sludges, residual chlorine, specific conductance, flow measurements and
microbiological tests are exempt from detection limit calculation requirements.
dnr.wi.gov/regulations/labcert/documents/guidance/-lodguide.pdf
THANK YOU FOR YOUR HELP.
Why down lodging bloked
Could you please upload this excel files in description box?
x axis is absorbance y axis is concentration, calculation and graph completely wrong also music is terrible
Depending on the country, it is correct
Terrible music.. please stop the music..
i think u mean 10/10