Instead of only pointing out the minor mistakes in this video, you could also praise this man for explaining difficult subjects so easy for us to understand. Keep up the good work and we will keep the A grade at the biochemistry!!
Lucid explanation. But there is a small correction I would like to add. The direction of the last primer of lagging strand will be reversed(i.e., 5'--> 3'). :)
Excellent video! You posted it in just the perfect time too. I'm studying for my Biochem final exam right now, and this has been super helpful to visualize everything!
ur videos r too helpful for me...when i hv confusion about any topic..i alwys search ur videos....ur speaking is also very fine...u alwys use easy words in ur sentence
I found one mistake, but in common your lessons are helping me a lot (right now) to pass the exams for the magister program. Thank you so much for your hard work!! I found all I needed and even more. Priceless material if you're tired of reading books (temporarily), and there is a need of human touch :)
I don't think that is correct... in the second part of the video the primer in the loop…when you imagine you straighten the loop out... than the primers in both chains will be in the same direction….or…leading strand is 5‘ (on the left) - 3´(on the right) so lagging strand is 3‘ (on the left) than on the end of the loop it should be 5‘ ....but primer on it starts also with 5‘…Still thanks for your videos, your teaching helps me a lot!
Barbora K the part which is incorrect i agree, but the idea is making a loop only in the direction of 5'-3' which justifies the loop formation. Don't bother about the rest loop, the dna polymerase only select the same direction primer. If you're not satisfied with it watch the video which describes it. I was also confused because of this one. But now I've got it.
Perfect Sir!! You resolve a huge problem I had nerver understanding how the process goes, and now I understand it! Thank you, Thank you, Thank you! May Allah bless you, secure your knowledge and your simple yet complete teaching methode!
The direction of the addition of primer is wrong in the lagging strand. Primers are added as soon as the helicase unwinds and multiple primers are added to that strand from the region of unwinding .......correction required.
frstly, formation of loop isn't clear to me still. n secondly, primers r removed by RNase H n den d gap is fulfilled by DNA pol 1 . dis portion isn't clear.
Is it true that there are leading and lagging direction at both strands?I mean in total we have a leading and lagging direction at leading strand & another leading and lagging direction at lagging strands?
thank u sir yesterday i got my result & i clear molecular biology paper with good marks thank u very much sir your videos are very helpful & i am doing M.Sc. Biochemistry
Formation of loop in the strand is not clear to me, and the direction of the lagging stand is wrong as you described, But rest of the videos I watched are very easy to understand
Firstly I would like to thanks for all your videos but I have a little bit problem in formation of loop on lagging strand plzzz u explain it clearly if u do I am thankful to you...
Because of the some writings seen in the bottom of the video, I can't see the figure clearly. Anyway good information you are great your videos are really helping me for my exams . Thankyou Sir
Sir i have a confusion that loop will not formed over becoz bacterial dna is circular so lagging strand will remain circular ,secondly okazaki experiment does not support loop formation...pls make clear my concept
How leading and lagging strands can form in same direction ???? Direction of primer is opposite to leading strand primer ??? I am able to understand this sir.. otherwise you are fantastic
thank you so much sir this video helps me a lot many confusion were cleared but sir can you tell me what was the direction problem in lagging strand because i confuse there please reply
Thanku Bhagwan ji💓🌼😇🌈🤗🙏Thanku sir🙏😇🌈🤗💓🌼
Thank you so much for appreciating my efforts
Instead of only pointing out the minor mistakes in this video, you could also praise this man for explaining difficult subjects so easy for us to understand. Keep up the good work and we will keep the A grade at the biochemistry!!
Thank you so much for appreciating my efforts
True that
Especially without editing anything in just one shot. That's impressive
But wrong is wrong.
@@palakkhandelwal8487 Go and then rewatch the video. It'll be clear thereafter.
Your video's are very knowledgeable as you go deep into the concepts and mechanisms. Love to watch and gain knowledge from your videos.
Glad to hear that you're getting benefit from my lectures
Lucid explanation. But there is a small correction I would like to add. The direction of the last primer of lagging strand will be reversed(i.e., 5'--> 3'). :)
with your explanation, this mind blowing topic become so easy to understand...thank u for such great content to reply on.!!!
You're welcome. Glad to hear that you're getting benefit from my lectures
Can't believe it is 7 years old video, 😮so much detailed explanation ❤❤ thanks sir
You're welcome
Aatma tript ho jati h aapse pdne ke baad🙏You are a gem❤️
Excellent video! You posted it in just the perfect time too. I'm studying for my Biochem final exam right now, and this has been super helpful to visualize everything!
Your explanation is far more better then my professors....good job keep it up
Thank you. Glad you liked my lectures. Please subscribe and share
ur videos r too helpful for me...when i hv confusion about any topic..i alwys search ur videos....ur speaking is also very fine...u alwys use easy words in ur sentence
This has helped me a lot.Such a great initiative to clear out the doubts of many who are struggling like me.
Glad to hear that you're getting benefit from my lectures
Thanku so much brother...u r a best soul...I don't have a best teacher like you....
you are a genius sir
Thank you
I found one mistake, but in common your lessons are helping me a lot (right now) to pass the exams for the magister program. Thank you so much for your hard work!! I found all I needed and even more. Priceless material if you're tired of reading books (temporarily), and there is a need of human touch :)
I don't think that is correct... in the second part of the video the primer in the loop…when you imagine you straighten the loop out... than the primers in both chains will be in the same direction….or…leading strand is 5‘ (on the left) - 3´(on the right) so lagging strand is 3‘ (on the left) than on the end of the loop it should be 5‘ ....but primer on it starts also with 5‘…Still thanks for your videos, your teaching helps me a lot!
Barbora K the part which is incorrect i agree, but the idea is making a loop only in the direction of 5'-3' which justifies the loop formation. Don't bother about the rest loop, the dna polymerase only select the same direction primer. If you're not satisfied with it watch the video which describes it. I was also confused because of this one. But now I've got it.
Major confusion is created
Yes u r correct in this video the direction of primers in the lagging strand is altered
I'm also agree with you after looping both replication direction will be same
Thank you sire. You are the real gem for all life science students. Lots of love and respect from assam.
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Perfect Sir!! You resolve a huge problem I had nerver understanding how the process goes, and now I understand it! Thank you, Thank you, Thank you!
May Allah bless you, secure your knowledge and your simple yet complete teaching methode!
+Kaouthar Najim thank you. Glad you liked my lectures
Meko nhi aaya smj..!! Plss help mee...! Mera test h nd yeh ques aane hi vaala h for sure...! Help me..!
sir i think there is some thing wrong in this lecture please rectify....:)
Thank you so much sir for such a informative video ...i was always afraid of this topic , but you made it like a childhood story
it is soo well explained!!
Great explanation as always from Shomu Sir!
I hope I master this concept and answer all the questiona from this topic
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First part of the video initiation was helpful...But the elongation part is not clear
U made it easier to understand much more 😀❤thank you.....
Glad to hear that you are getting benefit from lectures
Thank u very much sir ...
Ur teaching method just amazing....👍👌
You're welcome
Thank you so much sir
This is so helpful..
Lots of love and respect from Kolkata
Sir there is just no doubt after seeing your lectures..
You're welcome. Glad to hear that you're getting benefit from my lectures
Many thanks for all your great videos. your explain is wonderful and that attractive me to watch and watch without stopping.
Boss তুমি সেরা! ❤️❤️
The direction of the addition of primer is wrong in the lagging strand.
Primers are added as soon as the helicase unwinds and multiple primers are added to that strand from the region of unwinding
.......correction required.
No the direction is correct.
@@bogdanbogdanovich140 after the formation of loop the direction of dna synthesis is still 3 prime to 5 prime that is incorrect. see the video again.
@@kuldeepgupta8521 I'm afraid you don't understand that prime ends are actual ends and not directions. Technically it is correct.
No, in the video it is wrong totally
@@bogdanbogdanovich140 i agree with you
sir plz check the legging strand and primer direction on this and dna direction also
Directionality of legging strand is not right explained..but over all perfect..helped me to solve a lot of problems...thx u sir
sir thank you for such a detailed explaination.
You're welcome
Sir... Thanku frm core of my heart💝✨
Thank you so much for appreciating my efforts
Well explained sir, I like your videos 😀
Thank you
frstly, formation of loop isn't clear to me still. n secondly, primers r removed by RNase H n den d gap is fulfilled by DNA pol 1 . dis portion isn't clear.
Primers are removed by DNA pol 1 by it's 5'-3' exonuclease activity.
This video is so so good and really helpful.
You're welcome. Glad to hear that you're getting benefit from my lectures
But thanks for this video because I was totally blank in my class after this but you have cleared me a lot
Well now you know. Thank you. Glad you liked my lectures
You explained it so nicely 🥺thankyou sir
You're welcome. Glad to hear that you're getting benefit from my lectures
very very informative video
Thank you
Sir circular dna mai bhi loop bnega but kese ,? Pehle breakdown krna hoga dna ko
Thank you for clearing our concepts sir...
You're welcome. Glad to hear that you're getting benefit from my lectures
Tnqq sirr it's very useful 😍
You're welcome
Even difficult topics are explained in simplified manner🤗👍good.
Thank you so much for appreciating my efforts
excellent thank you sir
You're welcome
Excellent sir,i have to read the book only, once after watching these videos...too good to understand ,thnku
Glad to hear that you're getting benefit from my lectures
DNA is circular in PROKARYOTES
So how primer will bind at terminal site
You are right katta
Why DNA polymerase can't go in 3'-5'direction? Is there anything in it's structure?
Well explained sir ....Thank u
You're welcome
most beautiful video sir
your videos is very helpful f or my study ....thank you so much
Is it true that there are leading and lagging direction at both strands?I mean in total we have a leading and lagging direction at leading strand & another leading and lagging direction at lagging strands?
It is so easy to understand.... Thank you sir
You're welcome
Outstanding
nice explanation sir
thankyou sir
You're welcome
nice sir very helpful
You're welcome
Thanq dada.. I think ur 2nd diagram lagging stand loop is more perfect than first 1...
I just wanna be honest...
At the same time you make it easy to understand as well as confusing ( in the loop formation and replication direction ) ...
Loop formation is a 3d concept better visualized with animation
@@shomusbiologyofficial
Ya..
I watched it
Now i can understand how difficult to teach without animation
But you still did good job 👍👍
Link for animation plz
@@sankalpchowdhury1175
Thanks bcz...
thank u sir yesterday i got my result & i clear molecular biology paper with good marks thank u very much sir your videos are very helpful & i am doing M.Sc. Biochemistry
Thank you sir for ur teaching
thnk u so much sir & u r so cute😊
thanku so much sir..now my all doubt clear about elongation process in DNA replication
Very nice sir
You're welcome
Very understandable
Thank you
Formation of loop in the strand is not clear to me, and the direction of the lagging stand is wrong as you described,
But rest of the videos I watched are very easy to understand
Thank you for the lessons 🙏❤️
You're welcome
Very helpful vid ,thnkuu sirrr
Superb
You're welcome
I think primer in the lagging strand is not drawn in correct direction. its little bit confusing.. will u plz clear it?
Firstly I would like to thanks for all your videos but I have a little bit problem in formation of loop on lagging strand plzzz u explain it clearly if u do I am thankful to you...
direction of primer in lagging strand not clear
I love your tutorial sir
You're welcome
Because of the some writings seen in the bottom of the video, I can't see the figure clearly. Anyway good information you are great your videos are really helping me for my exams . Thankyou Sir
Turn off captions in settings
Dono strand ki polarity same kese ker dii Aap ny?
Sir please make a new video on this topic again , coz now u have more technology by which you will make it more easy to understand for us.
Okay. Sure
you have made similar videos on same topic so which one should we watch , the latest on or the earlier ones ???
Sir, I can't understand clearly about the looping in lagging strand
Mindblowing
thnk u so much sir for these videos its really helpful
+madhusmita pollai thank you. Glad it helped
Very helpful
Superb sir....thank u sooomuch
Sir, I have one question as in Ecoli there is circular DNA and already there is 3' hydroxyl group then why we add primer?
You are just awsm... thank you very much
Thank you so much sir
You're welcome
Very nice
You're welcome
Thank you
You're welcome
Superbbbb
Really nice👍...but one thing is a bit confusing dat is d dirctn of d arrows of the primers...
Thank you. There are some mistakes, hope i will do it correctly in my exams.
Yeah
Sir i have a confusion that loop will not formed over becoz bacterial dna is circular so lagging strand will remain circular ,secondly okazaki experiment does not support loop formation...pls make clear my concept
Sir you did a mistake
In lagging strand the primer is added in complement strand of 5' end .
Means at 5' end of the ssDNA the primer 5' to 3' is added
I don't understand what you are saying
Liked the way u explain bro 👌👌👌
Thank u sirr❤️
You're welcome
How leading and lagging strands can form in same direction ???? Direction of primer is opposite to leading strand primer ???
I am able to understand this sir.. otherwise you are fantastic
ThAnkyoU brother you helped me a lot.....
Perfect Sir!
thank you so much sir this video helps me a lot many confusion were cleared but sir can you tell me what was the direction problem in lagging strand because i confuse there please reply
everything is good but ...i think some may also need written notes of the whole described process of u sir...like i do..😄😄😄
Try to make notes on your own...it will help you more to remember the process..
Bro...I didn't get well about the looping of lagging strand......
Rewatch the video and Copy down the notes on this Chapter given in the description box. It'll be clear to you I think
you are amazing... thank you
the direction of the primer in the end of the lagging strand before the formation of the loop is not correct i guess..please correct that
Sir your reaction at 11:29 🤣🤣🤣🤣🙏🙏🙏🙏🙏
love your videos, they are always helpful:)
Thanku shomu sir
You're welcome