Fluorescence activated cell sorting (FACS)
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- Опубліковано 7 лип 2015
- This lecture explains about Fluorescence activated cell sorting better known as FACS. This is a technique for cell sorting using fluorescent probe.
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Thank you for watching
I'm yet to meet a person on this planet, the milky way galaxy, that's completed their degree without Shomu! :) Thank you!
+Anything Goes glad I can help
im 30 seconds in and already know this is gonna be super helpful for my technique write up paper in developmental biology. thanks!!
Thank you so much shomu bro...
very nice explanation
Thank you very much for this good explanation!
I have one question: Where exactly is the difference between die amount of proteins and the number of cells expressing this protein. Is this about how much of this proteins are on the cells, so more about the quality?
Thank you so much for sharing this really clear video
You're welcome
Just to clarify his explanation is wrong. First the cells that have the florescence will get charged with an electrical charging ring, they are then differentiated by electrostatic deflection. they are not naturally charge they acquire a charge to differentiate the ones that do and don't have the flourecent antibodies
you must know about Mr. hemant ....he is CEO OF a leading startup FLOW Jo in middle east and south east asia.....EXPERT of FLOW cytometry........MSc LIFE science fron JNU,.......PhD from max plank germany......Post Doc from chicago university..........
You are right
Sir, how many cells can be sorted by charging method in this technique ?
This video was too helpful Sir.
You're welcome
May i know that is this answer for flow cytometry and fluroscence
Sir please give lacture on the quenching fluorescence and phosphorescence please
very good
This is how the cells get charged...
The flow chamber, and the flow stream, is charged (± v where v typically lies between 50 and 150 V) at the moment a cell of interest is inside the droplet . The stream of droplets passes through a pair of charged plates (± 5000 V) so that the charged droplets are deflected and collected together with the cell container
If a potential difference is applied on the flow chamber containing all sorts of cells then the whole stream of droplets will be charged right? Why only the cell of interest? The more complex way is that the cell of interest is charged based on the scattering where the pattern is detected and when the flow stream exits forming droplets at the bottom then they are charged accordingly with an electric ring present, then they are separated with the help of charged plates in the end.
incredible teaching ability
Thank you
Sir ..you r always ..a saviour ... Love u sir .. u make the topic soo easy to get understand ... ❤️❤️ Love from heart
Thank you so much for appreciating my efforts
thank you sir
How do the different cells get the different charges to seperate them in the electric field?
awesome job bro ,,,keep it up ..ALLAH bless u
You can make topic understand for everyone 😉
You're welcome
Excellent explanation, salam amelicum❤👍
Thank you
My dear there is no use of radioactivity in flow cytometry. Please correct your sentence. We use fluorescent proteins or dyes but no radioactive material
+Hemant Agrawal so FACs just record the fluorescence of the tags or flourochromes attached to the cells right?
+Pranjal Dhaka
In flow cytometry, cells or cell like particles can be analyzed based upon their physical properties like cell size and cell complexity plus cells of interest can also be identified in a mixture by tagging its specific markers (surface/intracellular) by antibodies labeled with fluorescent tag. Sometimes cells can also be studied using fluorescent dyes without any antibodies for example viability assay, side population determination etc.
Please see a link, where flow cytometry is explained in a very nice way
www.d.umn.edu/~biomed/flowcytometry/introflowcytometry.pdf
PRANJAL DHAKA you must know about Mr. hemant ....he is CEO OF a leading startup FLOW Jo in middle east and south east asia.....EXPERT of FLOW cytometry........MSc LIFE science fron JNU,.......PhD from max plank germany......Post Doc from chicago university..........
Great to see u Sir
Thank you,good for you👏👏👏
You're welcome
Thank you so much sir 🙏🙂
You're welcome
very useful for beginners
You're welcome
R u with me I m going to xenotransplantation process practically from monkey genes to human tell what are the steps involved in that
thank u,so much Sir
You're welcome
GAL4/UAS.... Please ..... Upload.. This topic
Thanks bhai
Fan from Karnataka 😍
We wanna meet you
You're welcome. Glad to hear that you are getting benefit from the videos
Where are you from bhai?
Sir flow cytometry bhi ise hi bolte h kya sir plzz reply
Yes
Hi Suman, I have a question. I am reading a paper in which FACS was used and for a control of the experiment a batch of the cells was treated with 70% ethanol in order to kill them. They report the counting of the dead cells, but I wonder if it is still possible to detect those dead cells using FACS, since the membrane would be disrupted and the cell content would be lost. Any guess? Thank you.
+Marcelo Zerillo I think the purpose of the control in the experiment was to show the relative inability of the fluorescent tags to attach to the cells which got destroyed due to ethanol treatment. It still depends on the fluorescent tag used though because some tags attach to a specific protein under specific conditions,.
+Marcelo Zerillo
In Flow cytometry, people use viability dyes like PI, 7AAD, DAPI etc to detect dead cells. The principle is that dye gets bind to nucleic acids once the membrane is compromised and we can easily differentiate between dead and live . It has nothing to do with the non-attachment of fluorescent tags to the dead cells. Please see this link for a flow figure
www.uvm.edu/medicine/flowcytometry/documents/ViabilityusingPI_000.pdf
+Pranjal Dhaka thank you so much for your answer.
+Hemant Agrawal thank you so much for your answer.
The intensity is on the x axis. Not y
Having used the FACS machine for multiple years, I can verify that this information is incorrect. Also fluorescence is not radiation. You need to go back to school and pay attention
😂 something is burning
ur x and y axes are switched I think
totally wrong explanation of sorting..
+Suraj Saksena really? Please explain your points
I explained quite clearly where you were wrong over a year ago, and you deleted my reply, if you leave this intact, I'll gladly reconstruct my critique
@@2lefThumbs UMMM can I please get the correct explanation then please? I need it ASAP
Wrong.....👎🏿
you need to go back to school dear... why are spreading wrong information ... just delete it
Shomu's Biology, please delete your video because it's quite popular and spreads false information.