Thank you!! Really helpful, I was so confused by all the websites I used because they over-complicated the terminology and the diagrams! I spent so much time on those websites getting confused, when I could have just watched your video
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is called hybridization?
really helped!! thanks a lot I have a question: If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?
How is the complementary strand to the target sequence removed before hybridization? Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe
Sir apne life science ki coaching kaha se ki ti. Meri financial condition theek nahi h. But Mujhe coaching ki need h. Sir plz guid me for Jrf life science.
Wow this really helped alot! great way to explain, especially breaking it down with the "fish" and "bait", brought it into perspective... ONE QUESTION.. about the mRNA, you said it is useful for "Micro______ Technology, I cant make out what that was, sorry. Could you tell me what that was exactly because I'm still a bit confused on why mRNA detection is used and important. THANK YOU :)
measuring the level of mRNA, we are monitoring the activity of a gene i-e expression of whole genome. high level of mRNA means gene is expressing greatly.
This is how the person explain when he loves what he does .
thank you so much for the great explanation
All Respect
Thank you so much for appreciating my efforts
This is the third video I watch on this topic and you are the first to actually make it understandable, thank you!
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
This is what you call a good and simple tutorial. Thank you so much !
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you are tooo good.... A teacher like you is not even in reputed colleges... thank u so much.. keep going
Sir, u r one of my great teachers...my every problem has solution from ur lectures...
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Tomorrow is my exam, and you know what you are a great teacher and understand each and every thing you've explained, thank you so much ❤️
Thank you for appreciating his efforts
This is awesome. Why I can't find teachers like him¡¡ I understood all¡¡¡ Thank you so much. I will approve my exams now¡¡¡
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4:10 now that was a perfect circle 😳
Thank you
You are really master of microbiology
Thanks a lot for the videos 🙏
You're welcome
My favourite part of this video was the part where an ad popped up offering to do my assignments for me. I bet that will help my learning.
Aap jaise logon ki wajah se genetics interesting hai
Thank you
Sir, You're really something.... ❤🎉🎉🎉
Thank you so much for appreciating my efforts
Sir, i m pakistani and loves u sir for ur simplicity and teaching method. InshAllah in future i will come to india specially to meet u sir.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
AMAZING!! BETTER THAN MY SCHOOL TEACHERS!!! THANK YOU!!!!!!!!!
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Thank you so much... trying to prepare for exams. And your videos make them so much easier.
Thank you!! Really helpful, I was so confused by all the websites I used because they over-complicated the terminology and the diagrams! I spent so much time on those websites getting confused, when I could have just watched your video
You're welcome. Glad to hear that you're getting benefit from my lectures
i do study biology ( biochimestry )but in frrench luckely ur explanation was quite good i have understood quite well
NOBODY EXPLAINS ABT FISH BETTER THAN THIS VIDEO OF YOURS SIR THANK YOU SO MUCH
You're welcome. Glad you like it
Hats off sir! I am speechless that the way you narrate is amazing one.
Thank you. Glad you liked my lectures
God bless you. You make biology as simple as it should be. Thank you very much
Muchas Gracias Shomu!!! Saludos desde Paraguay
Amazing way of explaining sir.. Thank you sir for this 🥰
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excellent sir ..my doubts were cleared by your wonderful lecture..
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Thank you, Shomu!
It is easy because you explained it in simple way☺️
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Thank u so much sir . Aap n hote to pata nahi hum students ka kya hota.
You're welcome
Thank you Shomu!! Your lecture make it easy to learn, Big help!!!
Thanks man, you are great and i like your passion about this :)
Thanks! Most helpful information I found so far!
You're welcome
Thank you shomu...helped me alot,,, cheers from pakistan... :)
Thank you. Glad you liked my lectures
Helpfull for me all six lecture related to gene mapping......thanks sir .😊
Glad to hear that you're getting benefit from my lectures
Thanks brother.... U r a brilliant teacher
Nice one. I so much like your teaching skills
Thank you so much for appreciating my efforts
I have understood easily the complex thing from your lecture
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great job! your explanation is amazing
Thank you
dam that circle was perfect
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
You are a blessing !
Thank you
Great video, Shomu. Thank you!
You're welcome
Ur explainations are so worthy to listen
I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is
called hybridization?
Thanks a lot! Your videos are amazing!
You're welcome. Glad to hear that you're getting benefit from my lectures
Great job Shomu!
Thank you, all your videos are very helpful :)
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Thank you Shomu!
You're welcome
Thank you so much! It was so very hard to find a good video about in situ Hybridization. 🍀🔬📚💘
You r very helpful bro
Wonderful explanation Sir.
You're welcome
REALLY HELPFUL....... AWESOME LECTURE.... TY..
Glad it helped
You are amaizing 🌷🌷🌷🌷🌷🌷
Thank you so much for appreciating my efforts
really helped!! thanks a lot
I have a question:
If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?
U r gem to me and others too.
Thank you so much for appreciating my efforts
Many thanks, Shomu!!!
Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?
KOUA Patrick i think nick translation is used.
How is the complementary strand to the target sequence removed before hybridization?
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe
I hope you are doing fine! You are great!
You're welcome. Glad to hear that you're getting benefit from
It was very helpful bro ,thank you for making the video
Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?
Thank you
Very well taught
You're welcome
Good explanation skills! I like ur all the videos!
Sir is FISH technique used for serotonin binding receptorss???
Plzz answer
Google?
Hi Shomu! You are amazing!
Tysm🤓😊great video...👍
Glad to hear that you are getting benefit from the videos
THANKS SHOMU ;) ❤️❤️
You're welcome
Love you from BD🇧🇩
Thank you
Thank you. Plz how we can separate the strands ? in vivant
Superb Boss!
You're welcome
Very helpful video for mee😍😍
What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?
thanks .....great video as always..
how do you know the complementary sequence of the gene i.e. the probe or bait?
Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?
Dna sequence must be known prior to the experiment
You are right 👍 it is a easiest method.
PLEASE MAKE MORE!!!
Sure
Sir, is this same as southern and northern blotting techniques?
thank you, you were extremely helpful
Is it not same like southern blotting...?
What's the difference between these two..?
Thanks! nice & simple way to learn. How much work area is required for FISH setup?
FISH set up is basic and does not require much bench top space. a simple table top would work just fine.
Thank you ❣️
You're welcome
fantastic video, thank you so much!!!!!keep up the good work. Im subscribing already!
+-TIB5- thank you. Glad you liked my lectures
Thanks very helpful👍
Sir can you tell me which book it is present
very helpful this vedio for me ✌✌✌
how do u arrest cell in metaphase...when done on slide? reagent and concentration please...Nd please elaborate on signals .
We arrest them using freeze fracturing technique
Sir u r really 👍
Thabk you
Sir what about in situ hybridization using non radiolabeled probes??
Useful video....Thank u..
Thank you so so much ✨
You're welcome
Thank you so much sir
You're welcome
Mere exams h next month please ap kuch topics h unpe video bna skte ho plzzzzzzzzzzzzz mujhe smjh nhi aa rha kuch
soooo nyc bro.... help me a lotttttttt...... yaaaa a lottttttttttttt
Thaaank u a lot teacher
You're welcome
Sir apne life science ki coaching kaha se ki ti. Meri financial condition theek nahi h. But Mujhe coaching ki need h. Sir plz guid me for Jrf life science.
Watch my channel
Sir
How can we differentiate b/w ISH & FISH?
Both seem to be same
In ish, any type of probe can be used. But in fish, only florescent probe is used
well done bro...
Bro what's the difference between colony hybridization and insitu hybridization
very nice sir 👌👌👌👌👌
You're welcome
thank you so so much, I got it :))))
You're welcome
Wow this really helped alot! great way to explain, especially breaking it down with the "fish" and "bait", brought it into perspective... ONE QUESTION.. about the mRNA, you said it is useful for "Micro______ Technology, I cant make out what that was, sorry. Could you tell me what that was exactly because I'm still a bit confused on why mRNA detection is used and important. THANK YOU :)
+Lizbeth Marquez mRNA detection can be used to see if a gene of interest is expressed in a certain cell/tissue
Microarray Technology
measuring the level of mRNA, we are monitoring the activity of a gene i-e expression of whole genome. high level of mRNA means gene is expressing greatly.
Awsome sir but how know the gene sequence?
As i am pursuing PG.....wanna more elaboration of topics.......it wz lil bit helpful