just wanted to thank you again for always delivering the best and simplest explanation within the shortest time💕 i mean I won’t understand a thing in online classes (not bc my teachers are not good but bc i always manage to sleep) your videos are the only thing i ran into❤️ so much love for you and every single vid. esp during this pandemic!❤️
YOU ARE AMAZING!!! Keep doing this!! You guys like and subscribe, Im sure he is using his love for biology as a form of income on youtube, and these type of youtubers deserve Millions for what they are sharing. Thank you Shomu, you use visual thourough explanation, which I am very much appreciative of. You've helped me with so many of my courses
Very interesting! Could you please teach us about the luciferase element in the mrna Covid-19 gene therapy? Do you have any concerns about the 'vaccine' entering the blood stream? Thanks!
sir could you plz make more videos on the experimental parts like cloning western blotting explaining the role of each reagent used and steps to follow and troubleshooting part. it would be very grateful
Hello, I was wondering if I could have your email (not to be creepy) to discuss more on Green Fluorescent Proteins as my friend and I are trying to conduct a science fair project on this particular topic, but it is not in our current class curriculum and you seem to know A LOT about it. I would really like to talk in depth with you because, frankly, me researching (or trying to research) academic articles necessary for the science fair isn't making doing it nor am I learning anything new. Thank you!
If you would clone the bGAL before the GFP then they wouldn't be linked right? Because the bGAL gene has a stop codon in the end so it would make the GFP and bGAL separately? This is something that confused me.
There are lot of protein tags - Lets say V5, FLAG, HA You can tag a protein and then raise an antibody against the tag. This way you can visualize your protein of interest. (Short answer)
Sorry sir ...its obtained from Aequorea victoria ...speeling mistake becoz if someone making notes during exams like me than... Its of 238 amino acids not 328... U know all these things matters in exams ..and dont say stuffff and all during ur lectures becoz nothing is stuff When u explain in a good way every one like become like u ...
Such a passionate teacher :). u r a jewel to the youtube learning community. Thank you!
Glad to hear that you are getting benefit from the videos
My guy Shomo smartest man in world. Shomu for nobel prize of medicine 2022
Omg
Let's all just take a second to appreciate the PERFECT circle that was drawn at 3:38, bravo
Thank you
just wanted to thank you again for always delivering the best and simplest explanation within the shortest time💕 i mean I won’t understand a thing in online classes (not bc my teachers are not good but bc i always manage to sleep) your videos are the only thing i ran into❤️ so much love for you and every single vid. esp during this pandemic!❤️
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
You are blessing for us sir ❤
Thank you
All, in a nutshell, Thank you!
You're welcome
I adected somu's biology in the part my preparation.
Thank you so much......Sir
From telangana
You're welcome
Thank you sir ❤
YOU ARE AMAZING!!! Keep doing this!! You guys like and subscribe, Im sure he is using his love for biology as a form of income on youtube, and these type of youtubers deserve Millions for what they are sharing. Thank you Shomu, you use visual thourough explanation, which I am very much appreciative of. You've helped me with so many of my courses
Thank you so much for appreciating my efforts
I understand GFP now, cheers!
Dada thanks a lot.Finally amar concept clear holo.5 stars.
You are brilliant, as always.
thank for the lecture~ I really get the point how gfp tagging :D (always give your best!)
Thank you very much.
You are amazing sir
Thank you
sir ,thnkz alot ...very helpfull,,,i have a question...how does the GFP gene gets transcribed along with another gene,since it will be a foreign gene.
Brilliant video brother!
this video is amazing and very clear
Thank you. Glad you liked my lectures
Thank you
You're welcome
Thank you very much ,😍💖
This vedio is amezing, thank you
Thank you. Glad you liked my lectures
Thank you a very thorough explanation!
Very helpful info Soumo Da. Thank u ... Love from bengal ,,♥️
Thank you. Glad you liked my lectures
Discovered by shimomura... 😊
thank you so much for this video. very informative
9 years 😶😶 and still didn't get a better explanation of GFP 👍
You're welcome
this was great. Thank you and please keep it up
+John Mandrake thank you. Glad you liked my lectures
very help full. thank you.
Thank you for this video. Could we use the same method for mCherry tagging?
Very interesting!
Could you please teach us about the luciferase element in the mrna Covid-19 gene therapy?
Do you have any concerns about the 'vaccine' entering the blood stream?
Thanks!
Thanks sir
😄 😄 😄 😍 super
Thank you
5:10 Shomu Sir said "theek ache" in Bengali
😂😂😂 yess
**Aequorea victoria
Aquorea victoria****
😂😂😂 tumlog ko spelling bhi correct chaiye...jaha feea bhrte ho vaha toh koi kuch nhi pdhata ....ignore such mistakes.....
how does plasmid get into cell nucleus?
Nice one but can u upload the structure difference in GFP variants
Why I have lost sound on all of your videos but not other youtube videos? Did I make a mistake by subscribing?
sir could you plz make more videos on the experimental parts like cloning western blotting explaining the role of each reagent used and steps to follow and troubleshooting part. it would be very grateful
Later
Do u have video on mutant hunts and Reporter genes with respect to Transcriptional and translational gene fusion (operon and gene fusion with lacz)
Hello, I was wondering if I could have your email (not to be creepy) to discuss more on Green Fluorescent Proteins as my friend and I are trying to conduct a science fair project on this particular topic, but it is not in our current class curriculum and you seem to know A LOT about it. I would really like to talk in depth with you because, frankly, me researching (or trying to research) academic articles necessary for the science fair isn't making doing it nor am I learning anything new. Thank you!
What are fusion proteins containing enzymes
Can you please make a video of split ubiquitin yeast two hybrid assay. This is very useful for recent research.
your videos are very helpful.
sir ,can you do video of gene tagging in plant genomes
any notes for this
I need finanicial problem let me know what is the cost of making easier manually SCIF technique in determining cell genes in from monkey to human
Hello, who knows where I can get some GFP for viewing E coli cells please
Thankssss
sir is there any possibility that our GFP isn't inserted into the plasmid but the plasmid gets trasferred into the host?
Aye, if the experiment wasn't done right.
thanx dude
You're welcome
I really need vedio on gene tagging ,,, I am not getting the concept of that
Okay
If you would clone the bGAL before the GFP then they wouldn't be linked right? Because the bGAL gene has a stop codon in the end so it would make the GFP and bGAL separately?
This is something that confused me.
Someone can tell me please about current vaccination what is purpose of adding luciferase marker enzyme similar to the GFP thank you.
can you tell what are fusion protein tags? is this an example of fusion protein tag?
tell me*
There are lot of protein tags - Lets say V5, FLAG, HA You can tag a protein and then raise an antibody against the tag. This way you can visualize your protein of interest. (Short answer)
so the gene as well as the protein are both fluorescent?
No. Only GFP. It tells you the localization of the protein of interest.
Thank you dude you're great! keep it up! :-)
Ha Thik Ache 😁
*238 not 338
Is fusion proteins and recombination proteins are same? why?
please suggest answer.
Recombination proteins are different than fusion protein.
👍
You're welcome
I really don't understand how you know everything!!
I don't know most of the things
can you give a brief about plasmid and how it enters back to bacteria cells?
I hate yself because i cant understeand english soo well that I need to watch education videos :((( Stil learning
Not 328aminoacid .only 238amino acid
sir, the jelly fish name is Aequora victoria but you told Aequoras victoria.this is wrong
I can't concentrate with the phone ringing in the background
U need same focus sir had while explaining.....
When you record a clip turn off your cell phon
Sorry sir ...its obtained from Aequorea victoria ...speeling mistake becoz if someone making notes during exams like me than...
Its of 238 amino acids not 328...
U know all these things matters in exams ..and dont say stuffff and all during ur lectures becoz nothing is stuff
When u explain in a good way every one like become like u ...
Sir u r a biology don
You are a don.
Aequorea Victoria jelly fish
You are class apart...
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
CovidImages need to be invested more than half19
nåååhhhhh
Sir please can uh make a vedio on gene tagging
👍
Thank you