Actually the procedure itself wrong . This type of injection called as implantation The Implantation always should be done either Right side or left side flank subcutaneous region of mice, which is easy to take tumor volume.
Your mice are so calm when you grabbed them and injected. In our lab, we shoud have had anesthetized the mice then wait them for sleeping, or those energetic mice wuold twist themselves and try to run away. After that, we have to wait them recovering from anesthesia. So it always takes a long time to finish the xenograft. Do you train your mice to get used to injection or what to do if your mouse are too energetic to inject ?
According to my experience, the nude mice is calmer than hairy mice. So they will obediently until I finished the experiment without any treatment. :) But the hairy mice may not be the same. QQ However, subcutaneous injection is a simple experiment. For the experienced operators who can through the good restraint method without anesthesia. Except some very painful experiments, such as tongue injection, must be use appropriate anesthesia to reduce their pain.
dear michael, did you inject two different cell lines or cell line conditions in this mouse? because i want to do something similar and wanted to ask if this were different cells for each flanks. and maybe how well the tumor grows in such conditions. best regards pablo
Hi, Michael, How do you control the variance of tumor in these mice? Do you use 1/2", 0.5ml, 29 gauge syringes? Do you prepare 0.2ml cell solution then use the syringe to measure 100ul? Will 29 gauge shred the cells? Thank you!
to investigate the effect of X gene in tumor formation thus, we induce (overexpression) or inhibit (knock down) X gene in cancer cell then the cell inject into mice to observe tumor growth if it a cancer promoting gene (oncogene), the tumor of mice might be larger than control this is my first time doing this animal experiment with not a good restraint manner.... QQ
How do you feel when you do something so harmful to a poor animal like that I would feel disgusting with my self then someday get something like that done to me to now how they felt
i have nothing against experiments in rodents, because they are unfortunately crucial for clinical research. But it annoys me when people say things against it but still take pharmaceutical products which were tested using animal models
Not just pharmaceuticals. The pigments on the screen of your monitor or cell phone were tested on rodents for toxicity. Short and long term. In science, we do our best to use them rationally and painless. A video like this help us to learn the technique without using tons of mice.
@daniel gabriel because half knowledge is fatal. Immune cells behave different to cancer cells. Cancer cells can grow only in immune compromised mice. And it's better to experiment on an animal than on a human, unless you are a moron to hate your own species
@@shaktiprasadmishra1720 Actually, not entirely true, if you try to xenograft or allograft tumor cells, then yes, you need to have immunocompromised mice, but if you try syngeneic grafting of tumor cells from one strain to an immunocompetent host of the same strain, tumor cells won't be rejected.
Human nature, nothing you can do about it that will change, we will all only cease to be a part and only a part of society, nothing more, and it's also human nature to feel empathy, sorry for the rats.
Actually the procedure itself wrong .
This type of injection called as implantation
The Implantation always should be done either Right side or left side flank subcutaneous region of mice, which is easy to take tumor volume.
Well done! You are an amazing vet/researcher thanks a lot for doing it ethically and humanely and not scaring or torturing the mouse
Your mice are so calm when you grabbed them and injected. In our lab, we shoud have had anesthetized the mice then wait them for sleeping, or those energetic mice wuold twist themselves and try to run away. After that, we have to wait them recovering from anesthesia. So it always takes a long time to finish the xenograft. Do you train your mice to get used to injection or what to do if your mouse are too energetic to inject ?
According to my experience, the nude mice is calmer than hairy mice.
So they will obediently until I finished the experiment without any treatment. :)
But the hairy mice may not be the same. QQ
However, subcutaneous injection is a simple experiment.
For the experienced operators who can through the good restraint method without anesthesia.
Except some very painful experiments, such as tongue injection,
must be use appropriate anesthesia to reduce their pain.
Aspirating the cells in Insulin syringes, Cells may rupture or die.
Not the right way of implantation
What do you suggest?
There is a very serious operation error in this video. The needle was re-capped. Who taught you to do that?
Would you explain why the needle should not be re-capped?
@@SonVu-ju5ko risk of injuring your figure holding the cap.
@@coconutyummy8096 thanks a bunch!
Yeah, please don't do that. At least do the one-handed recapping.
Hi,
How long does your whole xenograft experiment take? from doing the experiment to phenotypic observation. just curious. Thanks!
dear michael,
did you inject two different cell lines or cell line conditions in this mouse?
because i want to do something similar and wanted to ask if this were different cells for each flanks.
and maybe how well the tumor grows in such conditions.
best regards
pablo
Hi, Michael, How do you control the variance of tumor in these mice? Do you use 1/2", 0.5ml, 29 gauge syringes? Do you prepare 0.2ml cell solution then use the syringe to measure 100ul? Will 29 gauge shred the cells? Thank you!
Mon Dieu quelle mauvaise contention.... miracle that u managed to inject
technique. mon cher. ce n`est pas mauvaise. Mauvaise est vos technique!
What is injection name
for what is this?
to investigate the effect of X gene in tumor formation
thus, we induce (overexpression) or inhibit (knock down) X gene in cancer cell
then the cell inject into mice to observe tumor growth
if it a cancer promoting gene (oncogene), the tumor of mice might be larger than control
this is my first time doing this animal experiment with not a good restraint manner.... QQ
+galaxymaster Cancer research
strawberry juice injection XD
Poor mice :'(
How do you feel when you do something so harmful to a poor animal like that I would feel disgusting with my self then someday get something like that done to me to now how they felt
how do you feel when you take your pain medicine or your flu shots, and support those experiments on animal?
i have nothing against experiments in rodents, because they are unfortunately crucial for clinical research. But it annoys me when people say things against it but still take pharmaceutical products which were tested using animal models
Not just pharmaceuticals. The pigments on the screen of your monitor or cell phone were tested on rodents for toxicity. Short and long term. In science, we do our best to use them rationally and painless. A video like this help us to learn the technique without using tons of mice.
What are you saying is extremely illogical and selfish since you harm poor animals to live (eating them - meds etc..)
CRUEL!!!!!!!!!!!!!!!!!!!!!!
it is. That is how the drugs are delivered to you after several rounds of testing in mice
@daniel gabriel I hope there is a haha reaction in UA-cam also
@daniel gabriel because half knowledge is fatal. Immune cells behave different to cancer cells. Cancer cells can grow only in immune compromised mice. And it's better to experiment on an animal than on a human, unless you are a moron to hate your own species
@@shaktiprasadmishra1720 Actually, not entirely true, if you try to xenograft or allograft tumor cells, then yes, you need to have immunocompromised mice, but if you try syngeneic grafting of tumor cells from one strain to an immunocompetent host of the same strain, tumor cells won't be rejected.
This is just cruel!
chloe dearan agreed. this is absolutely inhumane practice. it would seem in vitro research could provide similar research progress
If it makes you feel any better they're genetically engineered mice.
it's not true
@@greaterthanthegods2287 if only cells would behave similarly in vitro and in vivo
Evil bastards. I keep mice as pets, they are beautiful sweet little animals, this is disgusting
Do you think medications in pharmacies drop from the sky?? still believe in magics and miracles??
go home bleeding heart xD
It is sad that we have to do this, but the alternative--a stagnation in all medical science--is unequivocally worse.
Human nature, nothing you can do about it that will change, we will all only cease to be a part and only a part of society, nothing more, and it's also human nature to feel empathy, sorry for the rats.