Sir Aspirin and ibuprofen ki TLC prepare krne k liye bhi same solvent system (chloroform 5 ml , acetone 3ml ,Ethyl acetate 2 ml ) lena h jese paracitamol me aapne liya or inn sbki Rf value 7 k around hogi sir please answer krna practical exam h ????
Nahi. Molecule change hoga uske andr k functional groups change honge so apko solvent system change karna padta hain. Rahi baat exam ki to wo to aapko exam mein solvent system prepared hi milengi. So don't worry. Next Rf ka kaha aapne. Dear Rf value kabhi bhi 0.8 se zyada nahi hoti hain 0.2-0.8 ye ideal range hain. So acchhe se meqsument karna. All the best for exam
@@dr.jaivikshah2545 sir how to prepare solvent system if I want to do separation of falvanoids, alkaloids, and tannins etc from different plant extracts. Can you please tell. It's a really big help if you do 🥲👍🏻
Agr Aapko sabhi (listed molecules) ko ek sath karna ho to apko kafi trial & errors karne pdege Because sabhi ke structure mein polar and non polar groups hain. Aur agar aap separately Kar Rahe ho to aapko structure ko dhyan mein rakh Kar solvent select karna chahye. Hum kabhi common name se solvent decide nahi Kar sakte. For that we need exact structure of molecule
@@dr.jaivikshah2545 sir msc pass out freshers looking for assistant professor job practical knowledge mate kayo source/book follow kare? I am msc organic chemistry but physical chemistry na practical mate clarity nathi
Sir kinetic k practical ki ek confusion h please clear krde ...kl practical exam h K2S2o8 and KI practical me dono bottle mix krne k baad 10 ml flask me Lena or starch add krk fir titration krna h yaa flask me pehle cold water 20 ml add krne k baad fir ye solution 10 ml lekr then Na2s2o3 k sath titration krna h ??? means cold water kb or kitna add krna h sir aapki videos bhot helpful hoti h .. Kinetic k practical proper way se kahi pr bhi available nhi h.
Apki bat sahi hain ki kinetic k practicals nahi hain. Ye jo apka experiment hain usme 2 sets prepare Kar do uske baad jab mix Kar Rahe ho to turant apko titration karna hain. Ab rahi baat cold water add karna To dear cold water to kabhi add hi na karte hum. Ice add Kiya jata hain to decrease the rate of reaction So apko 3 4 small ice cubes add karne hain for each reading and then Ap 10ml solution lo fir starch add Karo uske baad titration karo aap. Hope it is clear to you Wish you good luck for exam.
sir plzz help me... पालक ki पत्तियों ka असेन्डिन्ग, दिसेन्डिन्ग or circular pepar chromatography or isi par thin layer chromatography... ka prectical bta dijiye...
It depends Selection of developing chambers depends on type of drug. You have to do trial and error If you are Research scholar then now a days UV chamber is used
See Lipids ho ya amino acids ho. Sb ki reactivity n property same nahi hoti so sab k liye koi common ratio solvent ka hum nahi keh skte. TLC jo hain wo apke molecule k structure pr depend krta hain. So apko study and trial dono karne pade. 1 baar me kabhi TLC nahi hoti dear.
Spot developer kaisy kaam krta hai??? I mean iss main aisa kia tha jo pehly spot nazar nhi aa rhy thy but sopt developer main khuch time rehny ky baad spot visible hua???
Mein apko itana suggest karunga ki aap ek baar TLC ki theory padho Apko waha samaj aa jayega ki spot developer ka importance kya hain and kyu hum iska use karte hain. Travel kitana hua plate mein base line se (due to solvent effect and interaction) wo identify karne k liye spot developer chahye hota hain... Hope its clear to you..??!!!
The basic concept/principle behind this TLC is same in all reference book. Working mechanism is also same. We performed this experiment as per Gujarat University syllabus.
So beautiful, so elegant, just looking like a wow 🫡
Nice and helpful video thankyou sir
Nice work sir
bhaayi tulsa ke patto se components alag karke dikha. ya bta kese lag honge , or kya kya saamaan chahiye ?
बहुत अच्छा गुरु जी....!!
Sir isepr work krne pehle dried krna hot air oven mein pr kitna time krna hai
Thank you for the information ❤️
Perfect video 📹 👌
Nice ( from Bangladesh)
Explain iodine chamber. Only iodine is taken in a closed container for locating???
There is no such any explanation of Iodine chamber. Its quite simple.
Yes you can use only Iodine crystal or you can add silica powder in it.
Amino acid na sample throw bhi spot thay ne jm k proline ,tyrosine ...?
Yes
Amino acids can also separate by TLC
Very nice information
Thanks
how chloroform is non-polar ? it has a polarity of 4.4
Excellent
Nice explanation sir
Solvent kya liya hai
Sir Aspirin and ibuprofen ki TLC prepare krne k liye bhi same solvent system (chloroform 5 ml , acetone 3ml ,Ethyl acetate 2 ml ) lena h jese paracitamol me aapne liya or inn sbki Rf value 7 k around hogi sir please answer krna practical exam h ????
Nahi.
Molecule change hoga uske andr k functional groups change honge so apko solvent system change karna padta hain.
Rahi baat exam ki to wo to aapko exam mein solvent system prepared hi milengi. So don't worry.
Next Rf ka kaha aapne. Dear Rf value kabhi bhi 0.8 se zyada nahi hoti hain
0.2-0.8 ye ideal range hain.
So acchhe se meqsument karna.
All the best for exam
@@dr.jaivikshah2545 Thank you sir😇
@@dr.jaivikshah2545 sir how to prepare solvent system if I want to do separation of falvanoids, alkaloids, and tannins etc from different plant extracts. Can you please tell. It's a really big help if you do 🥲👍🏻
Sir mujhe amino acid, sugar, lipid ke liye tlc karna hai to solvent kon kon se use kar sakti hu
Agr Aapko sabhi (listed molecules) ko ek sath karna ho to apko kafi trial & errors karne pdege
Because sabhi ke structure mein polar and non polar groups hain.
Aur agar aap separately Kar Rahe ho to aapko structure ko dhyan mein rakh Kar solvent select karna chahye.
Hum kabhi common name se solvent decide nahi Kar sakte. For that we need exact structure of molecule
@@dr.jaivikshah2545 ok sir
Beseline 2.5cm sy kam bhi ho skti hai kia??? I mean 2.5cm standard rkhi gai hai Chromatography main!
Standard to 1 cm hain
Na ki 2.5
But it all depends on size and dimension of your plate and beaker or jar you are using
@@dr.jaivikshah2545 ok thanks
@@dr.jaivikshah2545 sir msc pass out freshers looking for assistant professor job practical knowledge mate kayo source/book follow kare? I am msc organic chemistry but physical chemistry na practical mate clarity nathi
2.5 cm for paper chromatography.
To draw on What's man filter paper.
1cm for TLC
Sir kinetic k practical ki ek confusion h please clear krde ...kl practical exam h
K2S2o8 and KI practical me dono bottle mix krne k baad 10 ml flask me Lena or starch add krk fir titration krna h yaa flask me pehle cold water 20 ml add krne k baad fir ye solution 10 ml lekr then Na2s2o3 k sath titration krna h ??? means cold water kb or kitna add krna h sir aapki videos bhot helpful hoti h .. Kinetic k practical proper way se kahi pr bhi available nhi h.
Apki bat sahi hain ki kinetic k practicals nahi hain.
Ye jo apka experiment hain usme 2 sets prepare Kar do uske baad jab mix Kar Rahe ho to turant apko titration karna hain.
Ab rahi baat cold water add karna
To dear cold water to kabhi add hi na karte hum. Ice add Kiya jata hain to decrease the rate of reaction
So apko 3 4 small ice cubes add karne hain for each reading and then Ap 10ml solution lo fir starch add Karo uske baad titration karo aap.
Hope it is clear to you
Wish you good luck for exam.
@@dr.jaivikshah2545 Thankyou sir
sir plzz help me... पालक ki पत्तियों ka असेन्डिन्ग, दिसेन्डिन्ग or circular pepar chromatography or isi par thin layer chromatography... ka prectical bta dijiye...
I need to check it. Once I'll find definately share with you
@@dr.jaivikshah2545 thank u so much sir
For locating amino acids also iodine chamber is used???
It depends
Selection of developing chambers depends on type of drug.
You have to do trial and error
If you are Research scholar then now a days UV chamber is used
Solvent me benzene , acetone le skte h??
No. Benzene non polar hain n acetone polar so mix nahi honge dono
@@dr.jaivikshah2545 mera mtlb dono me se koi 1
@@anudwivedi_sanatani ji nahi aap kisi 1 ko use na Kar sakte. Mixture hi Lena pade
Sir lipids ka sample bolo na kaise Lena kitne ratio mein aur amino acid individuals konsa Lena purified pr which is name sit
See
Lipids ho ya amino acids ho. Sb ki reactivity n property same nahi hoti so sab k liye koi common ratio solvent ka hum nahi keh skte.
TLC jo hain wo apke molecule k structure pr depend krta hain. So apko study and trial dono karne pade.
1 baar me kabhi TLC nahi hoti dear.
@@dr.jaivikshah2545 ok sir
Biochemical tests for detection of aminoacids??? Give clue
Spot developer kaisy kaam krta hai???
I mean iss main aisa kia tha jo pehly spot nazar nhi aa rhy thy but sopt developer main khuch time rehny ky baad spot visible hua???
Mein apko itana suggest karunga ki aap ek baar TLC ki theory padho
Apko waha samaj aa jayega ki spot developer ka importance kya hain and kyu hum iska use karte hain.
Travel kitana hua plate mein base line se (due to solvent effect and interaction) wo identify karne k liye spot developer chahye hota hain...
Hope its clear to you..??!!!
@@dr.jaivikshah2545 ok
Bhai chloform polar hota hai na
@@danishroi6827 bhai
Chloroform ko aap kiske sath compare kar rahe us pr depend krega.
Solvent polarity order check krna apne aap ans apko mil jayega
Thank you for help ful
Sir mujhe project books ready karni hai
Good. Karie to aap
Sir thin layer ka experiment PDF d wara mil sakti hai
@@chintakumari8260 aap apna mail id muje share kar do
Mere paas jo hoga apko send kar duga
nice video
TLC plate jis chamber m rakhnai ka Tarika wrong h
Kiyu ki chamber mai jo compound rakha h, usko rakhnai ka Tarika bhi wrong h
@@harishchandraprajapati6772
Ji thik hain.
Aap sahi tarika bata dena aur dikha dena kese rkhte hain.
Shukriya
Carotenoids ka bhi batao na plzz
Sir lamda max ki practical dekhaiye plsss
It is a quite difficult to show you Lambda max experiment
But if i found definately share the link with you
Book name plz ma'am
thank you sir
Thank you sir & ma'am
What solvent you took
Its Ethyl acetate + Acetone + Chloroform
Well explanation
Thank you so much sir.
Blessed by your words 😇
Sir ત્રણ વખત spoting nu reason ના સમજાયું....
ત્રણ વખત એટલે કરવામાં આવે છે કે જેથી સ્પોટ તમે મૂકો એની સાન્દ્રતા વધી જાય જેથી કરીને તમને સારું developing જોવા મળે
Can u tell me which practical book r u preferaring for this
The basic concept/principle behind this TLC is same in all reference book. Working mechanism is also same.
We performed this experiment as per Gujarat University syllabus.
Sir msc k lecture bhi banao na
Thank you for suggestion and I assure you once I'll get opportunity definately will go for it.
It that you Biswa?
thanku sir
Hlo guys from lcop hasegaon
Wrong
Will you specify what you want to suggest as Wrong?
Thank you sir and ma'am