HPLC Chromatography Basics Explained
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- Опубліковано 5 жов 2024
- (You can also watch new HPLC video with demonstration here - 👉 • HPLC Chromatography De... )
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All you need to know about the HPLC Chromatography!
HPLC(High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture.HPLC separates and purifies compounds according to their polarity.
I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.
Later in this video, I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences.
Watch this video for more details.
Other Video Links:
ER TO GOLGI Transport: • Endoplasmic Reticulum ...
KDEL and KKXX Sequence: • KDEL and KKXX Signal, ...
If you have any doubts: Please leave a comment in the comments section below.
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I did B.Pharmacy but no body taught me like this during my college years. It was very difficult to understand by reading only theory. You have great knowledge mam and the way you explain is excellent. I am subscribing your channel now. I really appreciate your hard work. Thank you.
Hi even I completed B-Pharm are you still studying?
yes....An lg rha h Man k pass hee bsc MSc Or liya hota ..University prof..srf syllabus complete krne m believe krte😃😃😃
Agreed 👍
@@ranjanadevi7574 how about u? Even m doing masters in pharmaceutics
Heyy m also in my final year of bpharm
12mins and the whole concept of HPLC and Reverse Phase Chromatography were understood so beautifully ❤️
thanks mam,,, i have not comletely understand in 8 yrs in pharma industry and by ur video i just understand in 10 min ..... now i will not forgot this in my whole life....great job..
Thanks a lot Ashwani for kind feedback 😊
I think this is one of best explanation of HPLC on UA-cam...
Good lecture mem
Ya
Naa bhai 😊
Achaa HPLC pada rahe the kya 😂
@@diamondtrigger2311😂😂
I am a researcher, your video helped me to understand basics of HPLC. VERY GOOD TEACHING STYLE ! KEEP IT UP.
Thank You :) Happy to help
honestly no one can teach better than you. How perfectly you explain each n everything. Truly helpful and impressive
thanks a lot for making us aware about the basics of HPLC. Before meeting you, I never thought that a person could be a teacher, support, mentor and philosopher all in one! Thank you for all your hard work and dedication!
Thank you ma'am Tommorow is my environmental chemistry paper and I am going to write the same .....after watching chromatography videos and this one I feel it's simpler now
Thanks Harleen...and all d best for tomorrow..👍
@@BaaYo mam aik question hai answer kijya ga
How hplc differ form column chromatography
I m watching this video again after 3 yrs. And Thanks mam. i wish i could attend ur class in front of uh ..and. i deeply love with ur style of teaching
To the best teacher a student could ask for, I appreciate your creative and engaging lectures..Your passion for education is truly infectious..❤
Thank you dear ❤️
I don't find any reason to dislike such an wonderful lecture
As tomorrow is my exam. . . . . But m still watching ur video as my revision. . . . . . Coz u r a best teacher. . . . . Thanks a lot for ur work. . . . . 😘😘😘😘😘
The what and why for each step explained thoroughly
Thank you for this amazing lecture
Edible.oil.testing.method
Mene kbhi itni achi explanation nhi li kisi female teachers se u r the 1st one.... Well thanks ❤
you are a lifesaver, I have an exam in the morning and now I am all clear about every single detail. THANK YOU MAM
Which exam?
Lives simplified by your simple way of explanation . Amazing.
Excellent & Very Simple Clear & Nice Explanation Madam. Which is Very helped me to Understand clearly. I am Preparing for State Level M.Pharmacy Admission Entrance Exam i am also preparing for GPAT - Graduate Pharmacy Admission Test. Madam thank you very much for Preparation and Upload your Excellent Videos. These are very much useful to B.Pharmacy Students and M.Pharmacy Students who are Preparing Pharmaceutical Analysis Subject (Chemical Analysis).
Thank You mam.I think I just want to thank you and show my gratitude because your lectures help me a lot to crack my very first interview that I faced after the completion of my post-graduation.Your tutorials are extraordinary to understand.Things would become harder without your videos.Wish your success in every steps of your life ❤️❤️❤️
What u have explained in ur vdos are best to best ....
You're the best, you teach exactly what students need to understand unlike the rest of videos available here where they just talk bookish language
Tq mam ...tq so much.because of uh i can cover..... my +3 final year DSE-1 papper successfully 😍love from Odisha 😊
Jai jagannath 😊
Your lectures helped a lot in my studies... Please keep making these videos...😊👍
Where are you from
Mam you are saviour for us before theory exam 👍
Hats off to u.... You r leaving your child crying for making videos for us
Actually, that's what disturbs me most
Most easiest and valuable information about HPLC on UA-cam... respect to you mam 👏🏻👏🏻
Thank u so much ma'am 😊.. I was searching for this topic from long time and no one explained it in this cool manner . Firstly i thought u will not explain well but no other video explained as good as u ❤️❤️🙏. Thank u so much for your valuable time for us(students)
Thanks Prakash 😊
thank you so much mam for explaining in a good way
I got my job...they asked me about HPLC ...love from bangladesh 💓💓💓
wow congratulations !!
which company? where u come from?
Hello Ma'am! Yesterday, I saw your HPLC video for my job interview. To be honest, I swear on the name of God, for the first time in my life I have properly learned the concept of HPLC through your video. Lots of Love from Pakistan.
Thank you ma'am because I got job in ion health care baddi of himachal Pradesh, aap ne itna simple ho kr btaya Qa department m join kiya hun, mere pas thoda sa speak m problem h.... Mein Msc microbiology se 2015 kiya tha.... Thank alot ab mein sar utha kr chaluga thanks alot apka hr video interview prepared krne m bhut help kiya yeh meri pehli job h
Your lectures are very detailed and informative; you are a very good lecturer...keep up the good work!
Thank you ma'am for explaining in such an amazing way , this clear all my doubts.!! 💓
Absolutely amazing teaching. Very proper point to point knowledge. So much helpful and accurate. ❤️❤️❤️
Ma'am your teaching style is Unique and very much convincing.. I became die hard fan of your videos.. Please accept my regards, Ma'am..
Thank you ma'am .your teaching methods is amazing.l understood only in one seen .no one saw in my uni like you .
So simple and much effective explanation... Thank you so much
Thank mam
U are great
I want to know about more vedio
Like kf, gc, HPLC, and their tribal shooting
In industrial use
Thank you very much for your presentation. Would like to know about the buffer you mentioned to equilibrate before the sample run (getting the base line). can you explain more about the equilibration and the buffers to use thank you!
Great teaching, I understood even without knowing Hindi
This is very easiest method and very adorable i really like your teaching method thanks for your appreciation
I'm from Pakistan your lectures are very helpful thanks Madame.
Thank You Naseer 😊, glad to hear that my videos are being watched in neighbouring countries 🙏🏻😊
Ya
Me also
@@BaaYo yes mam your way of teaching is very good and easy....everyone can understand it....mam kindly also mention tge name of buffer solution and mqke video on chiral chromatography 😊 respect from pak
Mam.u r best teacher..u teach very well.and u r very pretty
Hi Guys, Please watch our new videos on
Mass Spectrometry ua-cam.com/video/w3LS22E10Zc/v-deo.html
UV- vis spectroscopy links.. ua-cam.com/video/1mrUHrZ-0zk/v-deo.html
Gas Chromatography ua-cam.com/video/0KqaIKHzHE8/v-deo.html
and please share your valuable feedback. Thanks.
U r cool..
Keep it up
Pls make a video to calculate peak area in HPLC and how to quantify?
Noted. surely will make one. thanks
zindagi me pehli bar koi cheej itne ache se clear hui hai
Thankyou mem such a great video fully understand in chromatography.
Me jab papr pura kiya tab 😀 baut khush hua tha . Finaly muje 70/68 mark mile mem biotech me 🙏 tnxx .
It was woow lecture simple way n easy. stay blessed mam.
Love you mam from Pakistan ❤❤
madam, in many cases multiport valve , buffer volume, pulse damper, n guard volumes are used . why?
Ma'am I really like the way you teach... Your way of teaching is just as beautiful as you❤️
I was very worried about my MSc Bioanalytical Science's , due to online studies i never understood the any chromatography techniques, but today only i found this channel i saw hplc video and now i am not having doubt..i will going to watch more videos, Requesting to please add Bioanalytical related topics..thankx
To access notes and practice questions, please join our Telegram channel t.me/baayo_official
Make Vedio on Isothermal titration calorimetry (ITC) and Differential scanning calorimetry (DSC)
I wish this had english subtitles :(
LMAO. I wasn't sure if she was jumping in and out of English or her accent was just that heavy. I thought she just had a heavy accent
Thank you Arti Ma'am,
It's very easy to understand if the teacher taught it in the way they themself understand.
Am seeing after 2 year... explained easy more than expecting .. its so simple after seeing this video .....🔥🔥
Super mam. ELISA per Video banav
Thanks laxmikant.. Jaldi bnaugi..:)
Hi mam, I want to quantify triphenylantimony from my sample, for that which mobile phase n which column is needed?
Maa'm plzzzz make A video On UV spectroscopy 🙏🙏🙏🙏🙏🙏 plz itsss request maa'm
Hi Lovepreet, Sorry for the delay. I was on break for two weeks, I will make video on this soon and upload it. Thanks for follow up.
Ha mam uv pe banaiye jaldi
Hi Prashant, UV Visible Sepctroscopy video is live now.
Watch it on below link:
ua-cam.com/video/1mrUHrZ-0zk/v-deo.html
Hi Lovepreet,
Based on your request, UV Visible Spectroscopy video is upload now :). Watch it at below link:
ua-cam.com/video/1mrUHrZ-0zk/v-deo.html
@@BaaYo thanks a lot maa'm 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏
Thankyou ma'am, you have explained the topic very clearly.. this video has made my concept more clearer... 😇
I have learnt so many things through ur video
I got placed after watching ur videos❤️my dream has come true. Thanks alot🥳
Jo ki hydrophobic the
Thanku so much ma'am
I just listen you're lectures chromatography & types of chromatography before last day of my exam I gain too much point easily & my concept is clear.
Thanku soooo much
This video is a boon for me because I was never able to understand biophysical techniques in class and eventually came across your channel and now everything seems sorted , thank you ❤️ Ma’am your teaching style is spectacular 👍
Hi
Mam want a video on centrifugation...you explain everything so easily
you are a great great teacher i learned from this video more than I did in university
I,m Chemist in Pharmaceutical Industry & perform HPLC. Your Lecture is very helpful 4 me
Lots of Best wishes from Pakistan. I love Indians teaching method.
In Pakistan we have floo fope method of teaching using slides
Thnk you mam
Im a big fan of you mam.u r explaining so simply each nd every topic.Ur lectures helping lot me to cover all topics
Wow mam ,i was in so much stress like there's so many things to study but now i have so much clearity thank you so much
thank you so much.. tomorrow is my presentation , this video is very very helpful for me
Thank you madam... Ur class made me understand the concept very clearly
Glad to hear that
I'm a pathology PG student, thank you so much for this... Even dacie or Tejinder don't have these explanations
Explained very well..better than our college teachers...must needed explanation for me...thnkq ma'am
Thank you very much mam.
What you have taught will remain permanently in my memory.
Thanks Olivia
You are the best discriber of instruments in my knowledge
Thank you so much Ma'am.... Allhamdulliah....I have learned a lot about HPLC after watching this vedio.... Mashallah.....it's just because of Allah almighty and then by your Wonderful lecture....
You are such a great teacher
May God give you many many success❤
mam u are nirraa ishq teacher what a beautiful explanation of hplc..kudos to your hardwork 💯
loved it..
itna clearly books me b ni milta aur na hi koi senior btata hai. kuch concepts tou mere aaj clear hue, 5 saal hplc chalane k baad.
Hello
@@rupalimahadik2424 helo
Aisi teacher kidhar milti hain... Humare Clg main to sab English ratke chale jate hain..
Best explanation👏🏻
Thank you so much ma'am ...maine aaj hi aapka channel dekha h aur aapne bhaut acche se smjhaya hua h .... Kl mera exam h ...m really very happy i was tired of reading the books n all stuff but this is very interesting ...🌺
Perfectly explained, I searched this content first time.
Thanks.🙏🙏
You have amazing Knowledge and great way to transfer this knowledge to others. Thanks , it will help me for M.PHRAMA
Hi , Hope you will be fine. I've recently completed my BS Zoology . In 8th semester we were studying a subject named Lab. Techniques. It contained the alomst 30 topics and i covered my most sylabs by watching your videos. How beautifully and simply you deliver your talk . I wish i can teach exactly like you. Love for you !
Explained in very well mannered way.. Thanks a lot mam. We really need genius like you. Love from Pakistan 💞
Thank you
Thank you soo sooo much mam🤗 because of you I can able to give my graduation exam tension free. 😊🙏
Thanku Mam A Lot of prayerz Love And Respect From Pakistan...Tomarrow is my Exams U made this easy 4 me
You took my heart..M soooooo happpppy. That I found your videossss...... sooooo Thankful to you. Maam
it's a conceptual video...I like ur explanation...u have explained every point here...😇
Crisp explanation of hplc.
Extremely helpful.Thank you Mam
Mam you describe so deeply that words getting bounce please keep it Short and easy
So amazing techniques of teaching
Very simply delivers all concept of HPLC , Thanks 🙏
thank you mam im student from final year b pharm . you explianed hplc in very simpler way just like a basic hint about it . really helpful as due to lockdown this online coaching cuase difficulty in understanding concept but you made it simpler . again nicely done
Remarkable way of explanation mam. Kindly upload lectures on Electrophoresis too. I couldn't finf any of your lecture on Electrophoresis. You explain each any everything very precisely. After taking your lectures not getting other lecturers .
Your explanaing way is Soo nice
Thank you so much ma'am for explaining HPLC so well.. you are great ❤
I watched your every videos about chromatography, and i glad to thanx for your excellent in explanation.
Thank you so much ☺
Thank u Yogesh
It was a very well put and precise in concept explanation, otherwise I have to run around searching for separate topics including the mechanism, principle and instrument layout
Today is my interview I hope this video will be help me for getting job
How was it?
First time i have seen huge likes on scientific instrument videos…
Keep on