HPLC Chromatography Basics Explained

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  • Опубліковано 4 січ 2025

КОМЕНТАРІ • 2,2 тис.

  • @GuptaGVlogs
    @GuptaGVlogs 5 років тому +18

    thanks mam,,, i have not comletely understand in 8 yrs in pharma industry and by ur video i just understand in 10 min ..... now i will not forgot this in my whole life....great job..

    • @BaaYo
      @BaaYo  5 років тому +4

      Thanks a lot Ashwani for kind feedback 😊

    • @badalchhatria9103
      @badalchhatria9103 2 місяці тому

      Dhyan se sunlee...bhai pharma industry mein ...kuch Isha badaa nahi hai😅😅😅

  • @anweshadas9611
    @anweshadas9611 3 роки тому +58

    12mins and the whole concept of HPLC and Reverse Phase Chromatography were understood so beautifully ❤️

  • @misbahmalik9752
    @misbahmalik9752 5 років тому +20

    honestly no one can teach better than you. How perfectly you explain each n everything. Truly helpful and impressive

  • @fatimarao793
    @fatimarao793 5 років тому +3

    Mene kbhi itni achi explanation nhi li kisi female teachers se u r the 1st one.... Well thanks ❤

  • @MuhammadHaroonAkram-r7l
    @MuhammadHaroonAkram-r7l Рік тому +1

    Hello Ma'am! Yesterday, I saw your HPLC video for my job interview. To be honest, I swear on the name of God, for the first time in my life I have properly learned the concept of HPLC through your video. Lots of Love from Pakistan.

  • @shoaibsheikh5206
    @shoaibsheikh5206 5 років тому +199

    I did B.Pharmacy but no body taught me like this during my college years. It was very difficult to understand by reading only theory. You have great knowledge mam and the way you explain is excellent. I am subscribing your channel now. I really appreciate your hard work. Thank you.

    • @ranjanadevi7574
      @ranjanadevi7574 4 роки тому +1

      Hi even I completed B-Pharm are you still studying?

    • @manisharajak6898
      @manisharajak6898 4 роки тому +1

      yes....An lg rha h Man k pass hee bsc MSc Or liya hota ..University prof..srf syllabus complete krne m believe krte😃😃😃

    • @muskanmehra9649
      @muskanmehra9649 4 роки тому

      Agreed 👍

    • @ankurpandey2713
      @ankurpandey2713 3 роки тому

      @@ranjanadevi7574 how about u? Even m doing masters in pharmaceutics

    • @parthsharma4081
      @parthsharma4081 2 роки тому +1

      Heyy m also in my final year of bpharm

  • @ReshamaChavan-x8w
    @ReshamaChavan-x8w 9 місяців тому +8

    I am a researcher, your video helped me to understand basics of HPLC. VERY GOOD TEACHING STYLE ! KEEP IT UP.

    • @BaaYo
      @BaaYo  9 місяців тому +1

      Thank You :) Happy to help

  • @saydfouz1942
    @saydfouz1942 4 роки тому +19

    thanks a lot for making us aware about the basics of HPLC. Before meeting you, I never thought that a person could be a teacher, support, mentor and philosopher all in one! Thank you for all your hard work and dedication!

  • @machindrasonawane559
    @machindrasonawane559 4 роки тому +160

    I think this is one of best explanation of HPLC on UA-cam...

  • @mdsadek1424
    @mdsadek1424 3 роки тому +6

    I don't find any reason to dislike such an wonderful lecture

  • @ankitamewara2649
    @ankitamewara2649 5 років тому +10

    Lives simplified by your simple way of explanation . Amazing.

  • @princesharma6166
    @princesharma6166 4 роки тому +4

    Most easiest and valuable information about HPLC on UA-cam... respect to you mam 👏🏻👏🏻

  • @drkiranpharmacy5114
    @drkiranpharmacy5114 5 років тому +4

    Excellent & Very Simple Clear & Nice Explanation Madam. Which is Very helped me to Understand clearly. I am Preparing for State Level M.Pharmacy Admission Entrance Exam i am also preparing for GPAT - Graduate Pharmacy Admission Test. Madam thank you very much for Preparation and Upload your Excellent Videos. These are very much useful to B.Pharmacy Students and M.Pharmacy Students who are Preparing Pharmaceutical Analysis Subject (Chemical Analysis).

  • @ankitthakor281
    @ankitthakor281 3 роки тому +1

    Thankyou mem such a great video fully understand in chromatography.
    Me jab papr pura kiya tab 😀 baut khush hua tha . Finaly muje 70/68 mark mile mem biotech me 🙏 tnxx .

  • @nishanttiwari8029
    @nishanttiwari8029 3 роки тому

    Thank you ma'am because I got job in ion health care baddi of himachal Pradesh, aap ne itna simple ho kr btaya Qa department m join kiya hun, mere pas thoda sa speak m problem h.... Mein Msc microbiology se 2015 kiya tha.... Thank alot ab mein sar utha kr chaluga thanks alot apka hr video interview prepared krne m bhut help kiya yeh meri pehli job h

  • @kidsofkhans6336
    @kidsofkhans6336 5 років тому +4

    As tomorrow is my exam. . . . . But m still watching ur video as my revision. . . . . . Coz u r a best teacher. . . . . Thanks a lot for ur work. . . . . 😘😘😘😘😘

  • @sushreepriyadarshanimahapa1766
    @sushreepriyadarshanimahapa1766 2 роки тому +4

    Tq mam ...tq so much.because of uh i can cover..... my +3 final year DSE-1 papper successfully 😍love from Odisha 😊

  • @Bhanuprakash-pe9so
    @Bhanuprakash-pe9so 6 років тому +8

    Thank u so much ma'am 😊.. I was searching for this topic from long time and no one explained it in this cool manner . Firstly i thought u will not explain well but no other video explained as good as u ❤️❤️🙏. Thank u so much for your valuable time for us(students)

    • @BaaYo
      @BaaYo  6 років тому +1

      Thanks Prakash 😊

  • @incognitoburrito8
    @incognitoburrito8 3 роки тому +2

    You're the best, you teach exactly what students need to understand unlike the rest of videos available here where they just talk bookish language

  • @sinister7592
    @sinister7592 10 місяців тому +1

    To the best teacher a student could ask for, I appreciate your creative and engaging lectures..Your passion for education is truly infectious..❤

    • @BaaYo
      @BaaYo  10 місяців тому

      Thank you dear ❤️

  • @sonalirp21
    @sonalirp21 4 роки тому +9

    The what and why for each step explained thoroughly
    Thank you for this amazing lecture

  • @harleenkaur436
    @harleenkaur436 6 років тому +11

    Thank you ma'am Tommorow is my environmental chemistry paper and I am going to write the same .....after watching chromatography videos and this one I feel it's simpler now

    • @BaaYo
      @BaaYo  6 років тому +2

      Thanks Harleen...and all d best for tomorrow..👍

    • @chemistrybymirha4480
      @chemistrybymirha4480 3 роки тому

      @@BaaYo mam aik question hai answer kijya ga
      How hplc differ form column chromatography

  • @abusaleh9528
    @abusaleh9528 3 роки тому +8

    Thank You mam.I think I just want to thank you and show my gratitude because your lectures help me a lot to crack my very first interview that I faced after the completion of my post-graduation.Your tutorials are extraordinary to understand.Things would become harder without your videos.Wish your success in every steps of your life ❤️❤️❤️

  • @rabiyamaqbool8649
    @rabiyamaqbool8649 3 роки тому +1

    you are a lifesaver, I have an exam in the morning and now I am all clear about every single detail. THANK YOU MAM

  • @chikoogupta899
    @chikoogupta899 2 роки тому

    zindagi me pehli bar koi cheej itne ache se clear hui hai

  • @bhawnaa_
    @bhawnaa_ 4 роки тому +6

    Absolutely amazing teaching. Very proper point to point knowledge. So much helpful and accurate. ❤️❤️❤️

  • @PrinciThapak_scienceelite
    @PrinciThapak_scienceelite 4 роки тому +8

    Your lectures helped a lot in my studies... Please keep making these videos...😊👍

  • @63-sayalimane7
    @63-sayalimane7 2 роки тому +3

    Mam you are saviour for us before theory exam 👍

  • @SheikhLateef
    @SheikhLateef 2 роки тому +1

    I m watching this video again after 3 yrs. And Thanks mam. i wish i could attend ur class in front of uh ..and. i deeply love with ur style of teaching

  • @sushantpople
    @sushantpople 3 роки тому

    I was very worried about my MSc Bioanalytical Science's , due to online studies i never understood the any chromatography techniques, but today only i found this channel i saw hplc video and now i am not having doubt..i will going to watch more videos, Requesting to please add Bioanalytical related topics..thankx

  • @sauravanand1738
    @sauravanand1738 6 років тому +6

    So simple and much effective explanation... Thank you so much

  • @avanraws77
    @avanraws77 5 років тому +10

    Your lectures are very detailed and informative; you are a very good lecturer...keep up the good work!

  • @varsharani9062
    @varsharani9062 4 роки тому +5

    Thank you ma'am for explaining in such an amazing way , this clear all my doubts.!! 💓

  • @BabyBoss-z8i
    @BabyBoss-z8i Рік тому

    Thank you so much Ma'am.... Allhamdulliah....I have learned a lot about HPLC after watching this vedio.... Mashallah.....it's just because of Allah almighty and then by your Wonderful lecture....

  • @harshmishra5372
    @harshmishra5372 3 роки тому

    Ma'am I really like the way you teach... Your way of teaching is just as beautiful as you❤️

  • @Aimenumme
    @Aimenumme 8 місяців тому +3

    Love you mam from Pakistan ❤❤

  • @farhanarashid3094
    @farhanarashid3094 5 років тому +18

    thank you so much mam for explaining in a good way

  • @muhammadnaseer5016
    @muhammadnaseer5016 6 років тому +8

    I'm from Pakistan your lectures are very helpful thanks Madame.

    • @BaaYo
      @BaaYo  6 років тому +2

      Thank You Naseer 😊, glad to hear that my videos are being watched in neighbouring countries 🙏🏻😊

    • @Impromptu21
      @Impromptu21 6 років тому +1

      Ya

    • @mirhusamuddinwasif929
      @mirhusamuddinwasif929 5 років тому +1

      Me also

    • @somalnisar6295
      @somalnisar6295 5 років тому +1

      @@BaaYo yes mam your way of teaching is very good and easy....everyone can understand it....mam kindly also mention tge name of buffer solution and mqke video on chiral chromatography 😊 respect from pak

  • @shuth6011
    @shuth6011 Місяць тому +1

    My concept is crystal clear now ❤

  • @RohitYadav-em8gf
    @RohitYadav-em8gf 3 роки тому +1

    Hats off to u.... You r leaving your child crying for making videos for us

  • @BilalAhmad-sr5uw
    @BilalAhmad-sr5uw 5 років тому +9

    Mam.u r best teacher..u teach very well.and u r very pretty

  • @nirajsalvi9786
    @nirajsalvi9786 6 років тому +8

    Thank mam
    U are great
    I want to know about more vedio
    Like kf, gc, HPLC, and their tribal shooting
    In industrial use

  • @rameezah9404
    @rameezah9404 6 років тому +6

    It was woow lecture simple way n easy. stay blessed mam.

  • @dharmendrachaudharydc5909
    @dharmendrachaudharydc5909 4 роки тому

    You are the best discriber of instruments in my knowledge

  • @lucienearth
    @lucienearth 4 роки тому +1

    Ma'am your teaching style is Unique and very much convincing.. I became die hard fan of your videos.. Please accept my regards, Ma'am..

  • @sailajamaddali3337
    @sailajamaddali3337 5 років тому +13

    Thank you very much for your presentation. Would like to know about the buffer you mentioned to equilibrate before the sample run (getting the base line). can you explain more about the equilibration and the buffers to use thank you!

  • @hasanshawon1421
    @hasanshawon1421 5 років тому +14

    I got my job...they asked me about HPLC ...love from bangladesh 💓💓💓

    • @BaaYo
      @BaaYo  5 років тому +5

      wow congratulations !!

    • @amitavsen8193
      @amitavsen8193 5 років тому

      which company? where u come from?

  • @pujakumari8787
    @pujakumari8787 5 років тому +7

    madam, in many cases multiport valve , buffer volume, pulse damper, n guard volumes are used . why?

  • @005abdulbasit8
    @005abdulbasit8 3 роки тому

    Thanku so much ma'am
    I just listen you're lectures chromatography & types of chromatography before last day of my exam I gain too much point easily & my concept is clear.
    Thanku soooo much

  • @ghulammuhammad5391
    @ghulammuhammad5391 Рік тому

    Thank you ma'am .your teaching methods is amazing.l understood only in one seen .no one saw in my uni like you .

  • @kiransg938
    @kiransg938 4 роки тому +3

    Hi mam, I want to quantify triphenylantimony from my sample, for that which mobile phase n which column is needed?

  • @laxmikantvharte5628
    @laxmikantvharte5628 6 років тому +5

    Super mam. ELISA per Video banav

    • @BaaYo
      @BaaYo  6 років тому +1

      Thanks laxmikant.. Jaldi bnaugi..:)

  • @omprakash3456
    @omprakash3456 6 років тому +8

    Make Vedio on Isothermal titration calorimetry (ITC) and Differential scanning calorimetry (DSC)

  • @harbalkiranjharbalkiranj9941
    @harbalkiranjharbalkiranj9941 3 роки тому

    Thanku Mam A Lot of prayerz Love And Respect From Pakistan...Tomarrow is my Exams U made this easy 4 me

  • @idreesMahsud
    @idreesMahsud Рік тому

    Lots of Best wishes from Pakistan. I love Indians teaching method.
    In Pakistan we have floo fope method of teaching using slides
    Thnk you mam

  • @biorainbows4313
    @biorainbows4313 6 років тому +28

    I wish this had english subtitles :(

    • @amorestperpe
      @amorestperpe 5 років тому +5

      LMAO. I wasn't sure if she was jumping in and out of English or her accent was just that heavy. I thought she just had a heavy accent

  • @lovepreetkaur6746
    @lovepreetkaur6746 6 років тому +6

    Maa'm plzzzz make A video On UV spectroscopy 🙏🙏🙏🙏🙏🙏 plz itsss request maa'm

    • @BaaYo
      @BaaYo  6 років тому +2

      Hi Lovepreet, Sorry for the delay. I was on break for two weeks, I will make video on this soon and upload it. Thanks for follow up.

    • @prashantjagdishkhedekar2895
      @prashantjagdishkhedekar2895 6 років тому +1

      Ha mam uv pe banaiye jaldi

    • @BaaYo
      @BaaYo  6 років тому +1

      Hi Prashant, UV Visible Sepctroscopy video is live now.
      Watch it on below link:
      ua-cam.com/video/1mrUHrZ-0zk/v-deo.html

    • @BaaYo
      @BaaYo  6 років тому +1

      Hi Lovepreet,
      Based on your request, UV Visible Spectroscopy video is upload now :). Watch it at below link:
      ua-cam.com/video/1mrUHrZ-0zk/v-deo.html

    • @amuandeep1240
      @amuandeep1240 6 років тому

      @@BaaYo thanks a lot maa'm 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏

  • @Impromptu21
    @Impromptu21 6 років тому +5

    Jo ki hydrophobic the

  • @anshikasharma7768
    @anshikasharma7768 4 роки тому

    Thank you so much ma'am ...maine aaj hi aapka channel dekha h aur aapne bhaut acche se smjhaya hua h .... Kl mera exam h ...m really very happy i was tired of reading the books n all stuff but this is very interesting ...🌺

  • @sindhuvelpula678
    @sindhuvelpula678 3 роки тому

    I have learnt so many things through ur video
    I got placed after watching ur videos❤️my dream has come true. Thanks alot🥳

  • @BaaYo
    @BaaYo  6 років тому +5

    Hi Guys, Please watch our new videos on
    Mass Spectrometry ua-cam.com/video/w3LS22E10Zc/v-deo.html
    UV- vis spectroscopy links.. ua-cam.com/video/1mrUHrZ-0zk/v-deo.html
    Gas Chromatography ua-cam.com/video/0KqaIKHzHE8/v-deo.html
    and please share your valuable feedback. Thanks.

    • @Kapil5462
      @Kapil5462 6 років тому

      U r cool..
      Keep it up

    • @prashantrajput4465
      @prashantrajput4465 6 років тому +1

      Pls make a video to calculate peak area in HPLC and how to quantify?

    • @BaaYo
      @BaaYo  6 років тому

      Noted. surely will make one. thanks

  • @--AgamdeepSingh
    @--AgamdeepSingh Рік тому

    mam u are nirraa ishq teacher what a beautiful explanation of hplc..kudos to your hardwork 💯

  • @zainabzakiahmad6963
    @zainabzakiahmad6963 2 місяці тому

    Thank u
    Hplc aapse asaan or acha koi explain nahi kr skta , na online na offline
    Thank u

  • @amitkboora
    @amitkboora 3 роки тому

    First time i have seen huge likes on scientific instrument videos…
    Keep on

  • @riteshmohanty5493
    @riteshmohanty5493 8 місяців тому +1

    What u have explained in ur vdos are best to best ....

  • @4horned_goat
    @4horned_goat 3 роки тому

    loved it..
    itna clearly books me b ni milta aur na hi koi senior btata hai. kuch concepts tou mere aaj clear hue, 5 saal hplc chalane k baad.

  • @goradhillon1993
    @goradhillon1993 2 роки тому +1

    This is very easiest method and very adorable i really like your teaching method thanks for your appreciation

  • @informative588
    @informative588 2 роки тому

    Explained in very well mannered way.. Thanks a lot mam. We really need genius like you. Love from Pakistan 💞

    • @BaaYo
      @BaaYo  2 роки тому

      Thank you

  • @manthanbarot5565
    @manthanbarot5565 7 місяців тому +1

    Best explanation ever 👏🏻

  • @syedmehtabgilani5188
    @syedmehtabgilani5188 3 роки тому

    I,m Chemist in Pharmaceutical Industry & perform HPLC. Your Lecture is very helpful 4 me

  • @jamyyy9523
    @jamyyy9523 4 роки тому

    Crisp explanation of hplc.
    Extremely helpful.Thank you Mam

  • @aishwaryashetmandrekar6537
    @aishwaryashetmandrekar6537 5 років тому

    Explained very well..better than our college teachers...must needed explanation for me...thnkq ma'am

  • @dnrfam8688
    @dnrfam8688 4 роки тому

    Im a big fan of you mam.u r explaining so simply each nd every topic.Ur lectures helping lot me to cover all topics

  • @YudhveerDeora
    @YudhveerDeora Місяць тому

    More than 6years since uploading this video , still the best explanation till now

  • @study_with_funn-u2e
    @study_with_funn-u2e Місяць тому +1

    Thanks mam clear all doubt ❤

  • @abusofian3316
    @abusofian3316 4 місяці тому

    it's a conceptual video...I like ur explanation...u have explained every point here...😇

  • @JYOtiRaNJanMANgaRaj
    @JYOtiRaNJanMANgaRaj 8 місяців тому +1

    THANK YOU SO MUCH MAM 😀🙏🙏🙏🙏

  • @Ali-id2gl
    @Ali-id2gl 4 роки тому +1

    Mam ! You are so intelligent.... thanks

  • @avaneetyadav2066
    @avaneetyadav2066 3 роки тому

    Am seeing after 2 year... explained easy more than expecting .. its so simple after seeing this video .....🔥🔥

  • @science_and_fun_
    @science_and_fun_ Рік тому +1

    Superb explanation ❤ thanks forever 😊

    • @BaaYo
      @BaaYo  Рік тому

      Glad you liked it

  • @saurabhsharma5403
    @saurabhsharma5403 10 місяців тому

    Wow mam ,i was in so much stress like there's so many things to study but now i have so much clearity thank you so much

  • @amardeepchaurasia7515
    @amardeepchaurasia7515 9 місяців тому

    You have amazing Knowledge and great way to transfer this knowledge to others. Thanks , it will help me for M.PHRAMA

  • @shubhangimogal8577
    @shubhangimogal8577 3 роки тому

    Wow mam really ap Jaise teachers ki bohot jarooorat he

  • @gavsraw7812
    @gavsraw7812 4 роки тому +1

    Perfectly explained, I searched this content first time.
    Thanks.🙏🙏

  • @Martis2002
    @Martis2002 3 роки тому

    Aisi teacher kidhar milti hain... Humare Clg main to sab English ratke chale jate hain..
    Best explanation👏🏻

  • @kunalchoudhary4144
    @kunalchoudhary4144 2 роки тому

    Mam your teaching is something different 🙂 ...

  • @sandrapinky6279
    @sandrapinky6279 Рік тому +1

    Thank you madam... Ur class made me understand the concept very clearly

    • @BaaYo
      @BaaYo  Рік тому

      Glad to hear that

  • @uniqueyoutuber1
    @uniqueyoutuber1 4 місяці тому +1

    Great teaching, I understood even without knowing Hindi

  • @SarimKiani-g2z
    @SarimKiani-g2z Рік тому

    You are such a great teacher
    May God give you many many success❤

  • @Laxmikumari-kx4px
    @Laxmikumari-kx4px 3 роки тому +1

    Thank you Mam you are a best teacher .

  • @anukrittimehra555
    @anukrittimehra555 2 роки тому +1

    This video is a boon for me because I was never able to understand biophysical techniques in class and eventually came across your channel and now everything seems sorted , thank you ❤️ Ma’am your teaching style is spectacular 👍

  • @akiakritigupta
    @akiakritigupta 4 роки тому +1

    Thankyou ma'am, you have explained the topic very clearly.. this video has made my concept more clearer... 😇

  • @mominurrahman9859
    @mominurrahman9859 Рік тому

    Hi, This video is really helpful for me. Keep making these types of resourceful video. Thank you so much.

  • @Muhammadsaleem-ki7br
    @Muhammadsaleem-ki7br 7 місяців тому +1

    Best to know the basic of HPLC

  • @ganeshsabale3600
    @ganeshsabale3600 4 роки тому

    Great mam ,u explained very simple manner,anyone can understand

  • @manalisingne6551
    @manalisingne6551 2 роки тому

    Thank you so much for making this vid ma'am... Best ever explanation on UA-cam 😃

  • @SufiyanAhmed-h3p
    @SufiyanAhmed-h3p 2 місяці тому

    Really ma'am you helped me a lot thank you for your brief explanation keep going .😊

  • @sunalipatyal9823
    @sunalipatyal9823 10 місяців тому

    One of the best Explanation Ever✨

  • @vishalkumarsingh6703
    @vishalkumarsingh6703 3 роки тому

    So amazing techniques of teaching
    Very simply delivers all concept of HPLC , Thanks 🙏

  • @darshanp1278
    @darshanp1278 8 місяців тому +1

    Very nice explanation..keep up doing good work..