Thank you so much! Highly appreciated! I can imagine, that you are very busy, but just know that you making those videos make a difference to some people and we would be happy if you would continue making this high quality contribution.
I was taught that you're supposed to pick two different restriction sites rather than the same one to make sure you keep the appropriate direction of incorporated gene. The way you presented means that some of the gene X fragments will be incorporated in the opposite direction, and without a possibility of selecting those out as they both would disrupt the tetracycline gene anyway, it seems useless to make such experiment...but nice effort!
Enjoy molecular cloning on your computer Download "Computer based exercises in Molecular Cloning Strategies" It's a set of nine exercises where you can simulate cloning and gene expression experiments in computer gumroad.com/l/zzDP
Video tutorials of cloning software Cloning software seem to be created for academia and industry research laboratories, however little effort is made to create exhaustive manuals and video resources that make it easy to use these software. Though few software do have elaborate manual accessible on internet, such as Vector NTI. Interestingly fewer efforts are made to generate tutorials for college/ University students to learn, how to work with these software. This will enormously build problem-solving capabilities in students. While learning about transcription, translation and recombinant DNA technology, students can actually perform experiments in simulated environment to learn to apply the concept in real life scenarios. I have pasted some of the online resources provided by companies and organizations that created cloning software. Vector NTI Advance® Sequence Analysis & Design Software Designing & Ordering Primers with Vector NTI® Software Vector NTI Express Sequence Analysis & Design Software ua-cam.com/video/o0eAe-a8g0Y/v-deo.html ua-cam.com/video/o0eAe-a8g0Y/v-deo.html Subcloning using Serial Cloner Collapse this post
Thank you so much! Highly appreciated! I can imagine, that you are very busy, but just know that you making those videos make a difference to some people and we would be happy if you would continue making this high quality contribution.
I was taught that you're supposed to pick two different restriction sites rather than the same one to make sure you keep the appropriate direction of incorporated gene. The way you presented means that some of the gene X fragments will be incorporated in the opposite direction, and without a possibility of selecting those out as they both would disrupt the tetracycline gene anyway, it seems useless to make such experiment...but nice effort!
Enjoy molecular cloning on your computer
Download "Computer based exercises in Molecular Cloning Strategies"
It's a set of nine exercises where you can simulate cloning and gene expression experiments in computer
gumroad.com/l/zzDP
Video tutorials of cloning software
Cloning software seem to be created for academia and industry research laboratories, however little effort is made to create exhaustive manuals and video resources that make it easy to use these software. Though few software do have elaborate manual accessible on internet, such as Vector NTI. Interestingly fewer efforts are made to generate tutorials for college/ University students to learn, how to work with these software. This will enormously build problem-solving capabilities in students. While learning about transcription, translation and recombinant DNA technology, students can actually perform experiments in simulated environment to learn to apply the concept in real life scenarios. I have pasted some of the online resources provided by companies and organizations that created cloning software.
Vector NTI Advance® Sequence Analysis & Design Software
Designing & Ordering Primers with Vector NTI® Software
Vector NTI Express Sequence Analysis & Design Software
ua-cam.com/video/o0eAe-a8g0Y/v-deo.html
ua-cam.com/video/o0eAe-a8g0Y/v-deo.html
Subcloning using Serial Cloner
Collapse this post