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Siddharth Srivastava
Приєднався 20 січ 2012
Videos on Molecular Cloning Strategies and Lab Math
Adjust reading frame, Subclone BKK56 gene in expression vector pGEX5X3
Adjust reading frame, Subclone BKK56 gene in expression vector pGEX5X3
Переглядів: 1 019
Відео
Construct pUC19 containing Green Fluorescent Protein GFP gene
Переглядів 1,2 тис.3 роки тому
Construct pUC19 containing Green Fluorescent Protein GFP gene
Directional cloning with compatible cohesive ends
Переглядів 4473 роки тому
Directional cloning with compatible cohesive ends
Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector
Переглядів 2063 роки тому
Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector
Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector
Переглядів 1483 роки тому
Directional cloning, Sub clone human erythropoietin gene from plasmid pTDL14 into expression vector
Directional cloning, using reverse complement function
Переглядів 2823 роки тому
Directional cloning, using reverse complement function
Express human erythropoietin gene as fusion protein
Переглядів 1153 роки тому
Express human erythropoietin gene as fusion protein
PCR based cloning, Clone Gene bbk56 from pTDL11 into expression vector pGEX3X
Переглядів 2193 роки тому
PCR based cloning, Clone Gene bbk56 from pTDL11 into expression vector pGEX3X
PCR based cloning, Clone GeneY from pTDL11 into cloning plasmid pUC19
Переглядів 1003 роки тому
PCR based cloning, Clone GeneY from pTDL11 into cloning plasmid pUC19
How to check your primers will work or not before ordering:experiment
Переглядів 52 тис.9 років тому
How to check your primers will work or not before ordering:experiment
Assemble restriction digestion assay
Переглядів 4,7 тис.11 років тому
How to assemble Restriction digestion assay. Calculation behind the assay system is explained.
Measure DNA by spectrophotometer
Переглядів 31 тис.11 років тому
Calculations to measure DNA by spectrophotmeter
Make Agarose gel for electrophoresis
Переглядів 6 тис.11 років тому
Make Agarose gel for electrophoresis
Import DNA into Serial Cloner software
Переглядів 11 тис.11 років тому
Import DNA into Serial Cloner software
Introduction to Cloning Software, The DNA Lab
Переглядів 34311 років тому
Introduction to Cloning Software, The DNA Lab
Import DNA Sequence into Cloning Program, The DNA Lab
Переглядів 30711 років тому
Import DNA Sequence into Cloning Program, The DNA Lab
Dload any course I need I ❤❤
Great !
Me gustpo
This video was very helpful.
they wrote to me that it cannot be obtained using a linear matrix product , what does it mean what should I do ?
Can you explain how to make TE 0.1X?
Why did you add gaga before primer sequence?
نريد الجلوس بجوار النبي صلى الله عليه وسلم في الجنة ....
Very well explained..but last point i did not get, how 1ul enzyme for 20U.
which software you are using
ua-cam.com/video/iDkRhjdkFU8/v-deo.html
Great insight. Thank you for the free lecture
Shouldn't the concentration of the buffer be 2? Since we want 2ug of DNA?
Thank you for this video, what a nice thing to do. from Australia a big thank you.
The process was explained to us in layman's terms. 🙏🏽 ❤️
Thank you Sir, I tried to practice but can't find the cloning vector pTDL14.
Please first try to speak little louder.we can't listen ur words properly.
Thank you sir for th explanation
Very well explained it was so helpful made my day😇
amazing.
Thanks for the video! This really helped me to understand this program.
Thank you so much
This video ruined my life
Sir I have to add EGFP gene and our own diff promoters in this. So how will it be done ?
Thanx sir for making such videos
pogchamp
I was taught that you're supposed to pick two different restriction sites rather than the same one to make sure you keep the appropriate direction of incorporated gene. The way you presented means that some of the gene X fragments will be incorporated in the opposite direction, and without a possibility of selecting those out as they both would disrupt the tetracycline gene anyway, it seems useless to make such experiment...but nice effort!
please can you help me sir ?
The video i was searching thank you so much
Great explanation.. crystal clear..thank u so much
Thank you for such a wonderful presentation
Thank you so much! Highly appreciated! I can imagine, that you are very busy, but just know that you making those videos make a difference to some people and we would be happy if you would continue making this high quality contribution.
very good job
the process is not working
You are teaching electrophoresis, so keep maths out as it is 6 std stuff
thank you
Thank you very much !
Excellent 👍.
THE COYOTE THANKS YOU!!!
Thanks Man! BIG HELP
Can you please send me the excel sheets.
This helped me tremendously, so thorough
Can you help me Sir ?
Sir ... please make some new videos
Thank you, it was useful
Thank you so much sir, Really helpful video. cleared my basics.
Thank you for explaining very thoroughly
it would be nice to see how to install it on linux
I need to install it on linux, I've tried to run with wine but it does not work.
You need to wake up first.
How the non identical part of the primers will be amplified?