DNA cloning and recombinant DNA | Biomolecules | MCAT | Khan Academy
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- Опубліковано 1 чер 2016
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Introduction to DNA cloning.
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I just realised.... these vids don’t have ads.... they still aren’t making no money off this.. god bless
yes thanks to not making any money we are successfully homeless .
What's wrong with making money off these tho? There are many useless videos making money off them, so why don't useful videos like these make money for the hardworking content creators?
Yeah, truely one of the best Not for profit organisations out there
@@Ariadne4 He's not saying ads are bad, he's saying no ads are good
Live Wikipedia
Sir, you are my favourite teacher on the Khan Academy platform. Great job !!! You helped me a lot during my studies
Thank you sir
who else has an exam in 2 days that they're desperately trying to cram for
I have in the next 5 hours
got a 96 on the final
@@Marie-qv6on congrats!
@@Marie-qv6on let's go! I have one tomorrow hopefully I'll understand the material well enough
@@miriamsthoughts how did it go😂?
how is he so smooth with the mouse?
he is using a drawing tablet ! :)
Bruh
That’s what I thought
Ohh, man he is using a writing pad.
Experience *pen
this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy
I wish my professor did the same thing to their lectures, would save me time reading textbooks
you should never replace reading the textbook, these lessons are complementary since they are simplified.
True i agree
Even 7 years later, he's still the best teacher to ever walk this Earth. Thank you so much for helping me during my studies!
Who else reads the comments while sal is explaining 😂
Great video, Khan Academy never fails to clear up difficult topics/processes.
That's amazing, to think we can do this inside cells using biochemistry to get what we want! Keep them coming Sal, loving these new (micro)bio vids.
gucci mane is a clone go look at him he is a human so yea do your research XD
This is genetics, not microbiology.
Umm i think to be precise its biotechnology-biomolecular.
THAT WAS AMAZING!!!
For a moment I imagined you teach us in our university, it would be like a dream 😢
Thank youuuu so much
You can hear the passion he has for teaching science in his videos. Amazing job!
i know right frfrf
Such a great video!! Explained this concept so clearly!
Bob Ross of biology :D
Even in med school, you're still helping me. Thank you!
You are Doing this stuff in med school?
@@paribhura6704 Lol unfortunately. We just started systems, but we covered this in our basic foundations 1st semester.
@@eniamrahc73 oh! We are doing a brief unit on it but I am in smiths grade, not many details
@@paribhura6704 just have to go back to this stuff again and again…..
Me too lol, first semester of med school, in 2 days I'll have my exam
this is good
Great video! Thank you, Sir.
May I say that It's pretty amazing how you're fascinated by science 😂 you transferred that to us too!
You just simplified the bulky and complicated material given by my lecturer.. Thank you so much ❤🎉
Thank you Sal. I wish every teacher is as awesome as you are
SUPER helpful content - thank you SO much!
thank you very much i wasnt understanding it until you explained
Very well done!! best explanation of this topic
Thank you for helping me through my lab courses. I would be lost without your videos.
Thank you! I was reading my textbook and lecture notes, but still couldn’t understand it. This video explained everything perfectly 👌
Got me through my assignment, thanks sir
This is the most concise and informative video on this.
Wonderful explanation!
That's the exact way of teaching....loved it
WOOOOOOWWWW OMGG THANK YOOOUU I was watching all of my videos from uni and reading all of my lectures...didn't get a thing.....and you are here making it so easy, you literally save me from resit.
Thank you very much .finally I got it.
thanks!!
Excellent Presentation. Very well done. The colours (against the dark background) make the intended impact.
Superb!! Very nice, simple explanation.
Excellent lecturer. Great clarity.
great explanation! Thanks!!!
Great vid Sal, you're the man!
started watching khan academy when i was in 9th grade
and hi i'm in my first week of college
turns out no professor teaches better than Khan
:)
Bro SAME! i also started watching since 9th grade and am now in my first year in college
I really love khan because learning becomes easier.
You make everything so much easier to understand
Well explained lecture Sir!!!!!!
His videos are life saver!!!
excellent tutor!
Thanks you have helped 😊
谢谢
Thank you so much
You are awesome really, very clear and good video, thank you so much, we will wait for more videos
make it easy to understand!! and it is almost identical to my lab materials but in simplify form!! Thank you!
You made my reading texbooks life easier
awesome video!!
Is there any videos about methods used to obtain DNA sequence?
Thank youuu !!!!
The first humans lived to be 900 years old now you see why its so important
Great explanation. Thanks a lot.
Tq so much for your kind information
Thank you Khan academy ❤️
brilliant
thank you 😃
Watched this video to understand what the term "DNA Cloning" means and how that's done with the help of recombinant DNA technology.
Got to know that it's reproducing of the desirable genes/sections of DNA with the help of certain restriction enzymes, DNA ligase, and the host bacteria that supplies plasmids to attach the target genes and then reproduces them.
This video also clarifies the question - "How to engineer microorganisms?" - for which I have seen other videos earlier (insulin production as a case in point)
Which biotechnology techniques can be used to proof if certain cell express a particular enzyme?
I wrote Elisa and Western blot.
Pls help?
Thank u sooooooo much . U saved a lot
와,, 이해하기 쉽게 설명 진짜 잘해주심 ㅠ
Thank you for the explantion🌻🌻
Thank you so much ❤
Awesome explanation. Thanks a loooooooooooot.
Thank you 🙏
Thank you very much your are agood teacher
Thank you 💓
Thank you
Man, thanks I love you.
could you make a video and explain different kind if cloning? Gateway, Greengate, Goldenste are some examples I can't differentiate from each other...
Thank you so much! I’m in biology D as a freshman right now, and with the whole online learning deal, I’m struggling in the class, and this made the whole idea wayyyy easier to understand! Thanks!
You would not even be doing this level of work as a freshman. It will probably be the first semester of your junior year before you start conducting these kinds of experiments.
@@gregsmith6756 whats the point of responding 2 years later? lmao
@@nitro.2405for others whom it may concern 😊
Thank u. 😊
thank you so much
The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.
Thankyou for the explanation
Thank u
Awesome
Pro you are amazing person..really you explain any object i want to study it ..i don’t know how i can thank you 😢❤❤❤
나 한국인인데 쌤 설명 지리네요
Thank you thank you thank you
Thank you for getting me through my studies! These videos really help piece it altogether.
Much appreciated🙌
Your Northern Irish student😁
Explicas de lo mejor , se me quedó todo!!! gracias .
You are the BEST!!!!!!!!!!!!!
Thanks
How do the proteins encoded by the new plasmid get expressed?
Thank you so much for making this video!!!!
What is the solution in the test tube made of?
Fascinating stuff. Thank you!
The drawing is great!
thank you didnt understand what the overhangs were and overall process of DNA cloning
Thanksss
I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.
Isn't bacteria a prokaryote and prokaryotes don't have introns.
Love the Star Wars reference
We love you Sal❤️💪🏾👑
the only good thing about youtube algorithm is that, it introduced me to Khan Academy
Love the vids. Can you record at just a slightly higher volume please?
I wonder how yoou look, you have a good radio voice
Homie mentioned the Clone Wars haha. They really know their audience
thnq ew sir.....
i really find it very much useful..
Which video describes the extraction of desired product? Insulin, for instance?
Holly Stoltzfus
You would just add a restriction digest that flanks either side of the gene of interest.
I am currently making a integrated test and man, you helped me a lot.
Explanation is so good :)
how do we know that the bacterial colony with the desired product only have the antibiotic resistance?
Because we are using plasmids which carry the factors coding for resistance against some antibiotics and the scientists who perform these experiments are well aware of the plasmids and the resistance they have ..and as the replication occurs in the E coli this gets amplified...