This is hands down the BEST video explaining the nucleophilic attack : i couldn't understand a thing until I found your video. So thank you thank you thank you !!!
with a polymerization rate of 1000 bases per second, and an error rate of 1 billion bases added/ 1 error, you can calculate an error rate of ~ 1 error every 12 days!! How crazy that is with 1000 bases being added every second. Mind blowing efficiency
Technically, it does not. All Nucleotide triphosphates are available. However, it usually only catalyzes the addition when the correct hydrogen bonding occurs. Basically, DNA pol catalyzes best when the base pairing is correct.
There has to be some necessity, some reaction, one process, one bond, one compulsion which makes this process sure shot ensuring 1 in billion accuracy.
RNA-DNA double strands have a slightly different conformation, which is needed for polymerase attachment. If it could attach to DNA-DNA double strands it could start where it is not needed.
RNA-DNA binds slightly differently than DNA-DNA and let's the polymerase attach where it is needed. There is a lot of double stranded DNA but the polymerase is only needed in certain places and times. DNA-RNA primer is one of those places.
This is hands down the BEST video explaining the nucleophilic attack : i couldn't understand a thing until I found your video. So thank you thank you thank you !!!
Thank you for the support and for watching!
Thanks alot bro, I have been searching for hours for a video that explains this 😍😍
I just signed in to say u r GENIUS, been watching many vids never got the concept really well like this
thank you
Glad to help and please share the video if you liked it!
you just helped a freshman with his hw thank you
Thank you, you’re the only video I can find hat explains this part!
with a polymerization rate of 1000 bases per second, and an error rate of 1 billion bases added/ 1 error, you can calculate an error rate of ~ 1 error every 12 days!! How crazy that is with 1000 bases being added every second. Mind blowing efficiency
It's so cool!
Very clear, perfect review. Thanks!
Glad it was helpful!
Many thanks - very informative with the molecular description.
You are welcome!
how DNA polymerase understands which base has to attach to the template strand?
Technically, it does not. All Nucleotide triphosphates are available. However, it usually only catalyzes the addition when the correct hydrogen bonding occurs. Basically, DNA pol catalyzes best when the base pairing is correct.
This is Gold
Thanks for watching!
There has to be some necessity, some reaction, one process, one bond, one compulsion which makes this process sure shot ensuring 1 in billion accuracy.
Science is so cool!
Excuse me do we consider this part in intitation or elongation??
Great video. Any idea where the new nucleotides come from that the DNA polymerase attaches?
They use "free floating nucleotides" which basically just float freely in the nucleus
@@marioscleaner Thanks... are they spontaneously generated in the nucleus?
@@PeteRoyJackson I think so, from synthesizing. Apparently they have to be available in the nucleus to produce mRNA strands
@@marioscleaner So that means they must appear essentially out of some background energy source?
@@PeteRoyJackson Yes, i suppose so
So clear thank you very much
Thanks for watching and feel free to share the video!
Sir , please tell me the name of experiment that prove the opposite polarity of DNA
Why polymerase 3 needs primers to starts replication or adding nucleotides????
RNA-DNA double strands have a slightly different conformation, which is needed for polymerase attachment. If it could attach to DNA-DNA double strands it could start where it is not needed.
How clever ,just how did this happen ?
Thankyou so much, you're a god.
Glad I could help!
why polymerase 3 need a primer to start replication ?
RNA-DNA binds slightly differently than DNA-DNA and let's the polymerase attach where it is needed. There is a lot of double stranded DNA but the polymerase is only needed in certain places and times. DNA-RNA primer is one of those places.
Why DNA is found in helix form not in stick form
Because the phosphate of the first nucleotide is attached to the the other nucleotide from 1st Carbon to the 3rd carbo
nucleoside triphosphate
thank uuuuu
Welcome 😊
💕💕💕💕💕💕
Ok
Thanks for watching!