Hemoglobinopathies, Hemoglobin Electrophoresis, and HPLC

This video briefly covers various hemoglobinopathies and how they are detected on hemoglobin electrophoresis and high performance liquid chromatography (HPLC). Pathology residents and laboratory technicians may benefit the most from this lecture, but the content should be suitable to all levels within the healthcare field with an interest in this topic.
10:55 High performance liquid chromatography (HPLC)
16:48 Hemoglobin electrophoresis*
*Errata:
Beginning at timestamp 26:28, the anode/cathode on the citrate agar (bottom gel) is reversed.
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[@milliefoley4543]

I finally understand hemoglobin electrophoresis. Thank you so much :)

[@nickianderson8619]

This was the best video ever!!!! I've been trying to find resources for Hb electrophoresis to help me interpret between the different haemoglobinopathies! This is a winner! Thanks so much!

[@emarupaul7492]

Very thankful for the wonderful and excellent work

[@odelism.d.301]

Fantastic video for upcoming boards! Thank you

[@harrypotter1096]

The best video of this topic!

[@user-tv6hq1uf7l]

Amazing video and incredibly helpful for boards!! Great Job and thank you!!

[@boyaksgurl]

This video is awesome!!!!

[@haidernisar7340]

Wow
Brilliant

[@user-ul5pt1yb8z]

Great work thanks a lot

[@vairagya108]

Thanks a million! First time ever that i understood this stuff! Thank you for the clear concepts. Thank you for making is easy to understand and remember (with the pneumonics!!) Super awesome! (Hope i pass my exam if this stuff gets tested!)

[@reading-channel]

thanks alot 🌺

[@adnan.q.753]

Hello!
For the question at 27:50 (Hb G trait), are the (+) and (-) signs in the right place on the citrate agar gel?
I am still confused about that question but if the (+) and (-) signs are switched, the question makes sense.
Also, this is by far the best video I have come across on this topic.
Thank you!

[@MohammadKhan-mv2hk]

great Vedio, was there a small mistake on the last electrophoresis ? the citrate when from negative to positive so I assume the bands were reversed no?

[@IbrahimHajjali]

You are absolutely correct, thank you! An errata was added to the description of the video.