This was very informative and clear! I am a college student taking microbiology and this helped make things more clear to me than reading from the manual. Thank you for this! :)
Her students are lucky. Many teachers are not that dedicated and therefore you may see their students working in an institution or company while they cannot even use a microscope properly!
I'm in HOSA and using a microscope with oil immersion is one of the skills we have to learn. I found this really helpful to visualize the procedure. Thank you!!
Thank you dearly for this video. It is 10 years later but you are teaching me now. I am on a mission to help our aquarium industry in South Africa, and your video contributes to it. Much love and thanks.
What a great lady. Thanks for doing this. Our teachers for this particular subject are very organised and helpful in general(hence the link to your video), but there are so many of our teachers who are not. Really chuffed and grateful that you cared enough to make this, and that you are able to demonstrate that you know about what you are teaching, and that you care about your students. Whilst the lecture at the beginning might not be important for those outside of your class, for me it demonstrates that you communicate, outline expectations, provide information and opportunities to master skills. I even bet all of your assessments come complete with marking schedules and correct answers that you use to mark them with, and you probably provide feedback. This is a novelty at our university apparently despite the HUGE EXPENSE. Anyway... thank you :) Do you teach vet clinic practice at all?
Thank you for the tutorial. I recently purchased a microscope, but it came with no documentation, so your video helped to give me a lot of needed information. I appreciate you taking the time to do this.
Hey I have a quesstion and since you're the first comment can you answer a question for me? in 9:22 She said dabbing it with a can wipe or something like that. Did i mishear? If so what kind of wipe do you have to dab it with?
Thank you so much for the details and the clear instructions how to use microscope to find the bacteria, fast . And thanks for your encouragement not to frustrated. But try hard , and to draw a circle, because the bacteria are so small, can’t be seen by naked eyes. And remember to put immersion oil only with 100x and lean focusing with fine knob.. you are amazi. I appreciate your explanation .
This is a highly detailed demonstration for using a microscope in a perfect way. I am a chemist and I have never used light microscopes ever in my life but in my research I need to use them a lot and a lot.. I have been searching videos like this on youtube to get familiar with the proper working of the microscope. I am really thankful to the instructor of this video for uploading this nice video.. Cheers!!!!! and Thanks a lot !!! :) :)
Just received my new microscope and found this video...the instructions with my Swift microscope are very basic and your video has helped me understand how to use all the components and accessories. Excellently informative video - thank you :o)
glad the advice in the video is explained in no uncertain terms. important to be very clear with these fundamentals, which are often difficult for students to grasp, myself included. microbiology is hard and it pays to be meticulous and really consider as many aspects of your technique as possible. you might think people are just being anal but, when you realise why, it usually makes sense.
Very useful! I'm using a microscope at home with my kids, and even though I have some experience in microbiology, you gave some great pointers! Thank you
This was helpful! I’m in microbiology at the moment and had a little trouble working the oil lens. This made it more clear on what to do if I lost the focus.
Thank you. I am not a biologist or a microbiologist but I am fascinated with the subject. I purchased a very nice Omax digital microscope and your videos are extremely helpful for the self learner.
I have such a problem with oil immersion! My professor won't help us focus it anymore, so I'm trying to learn on my own! I can find it on every objective but oil immersion. Trying to find out what I'm doing wrong. Thank you.
I thank you very much for teaching us how to do it. We have very stupid lecturers, very stupid demonstrators and they never showed us how to find a bacteria and how to do the the oil immersion. please keep posting videos like this.
This is exactly what I needed for me and my daughters to learn how to use the 100x objective lense and oil immersion. This only thing left out is whether to use oil/b or oil/a.
much aloha! I have a skills test and this is my first time using a microscope this semester! This made me a lot more confident and comfortable! Mahalo from Hawaii ^^
She explained it very well, but I would still be afraid to have her as a professor because I'm a bit slow, the beauty of videos is that you can rewind them lol
LOVE LOVE LOVE...!!! from a teacher who has no idea how we didn't destroy every single microscope we had in college. Master teacher, Thank You Dear = WOW
Thank you my grand father who is a scienceist got my a microskopt like this for my 12 birth day (augast 20 2012) and i just set it up and he and I didnot now how to applie the oil and now we do thank you very very much.
I wish my professor helped me like this video did. I can't wait to go to lab and try it. I only spent 2 freakin hours looking at slides without seeing anything! And my professors tip "walk away and come back to it, its there, you just aren't looking good enough" seriously?!
I usually have trouble finding bacteria. Do you think you could show us what to look for at the main objectives, 4x,10x, 40x, and 100x, and what exactly it looks like under the microscope? I’ve been told to look for grains of sand? If there is a certain way to prepare the slide, could you also include that? This would seriously be a great help!
Well done ! Thank's for your trick about the right condenser distance, I will use it and thank's again for the oil cleaning demonstration I kiss you Philippe
I had 3 Biologies in Ontario Collegiate Studies of CANADA!! My 1st MicroScope Lab Instrument was in Grade 10 Biology,then Grade 11 Biology & Finally Grade 13 Gold Honourary Collegiate ,Ontario,Sciences.Although I was already familiar with with my Laboratory $300.00+Taxes Edmund Scientific 3 Achromatic-MFg Multi-Coated Objective Instrument, I only got my SUPERB-Lab KONUS ACADEMY with Laboratory with "Oil Immersion in May of 2011! Today,Ontario,CANADA Biologies are Grades,10,11,12.Grade13removed!!
I been watching many tutorials about how to use the oil immersion objective but no one (not even at my school) put oil between the condenser and the slide, i learned about this some time ago, you need to do this to have maximum resolution and get a serious benefit about using the oil immersion objective, if you not do this you are going to be limited by the numeric aperture of the condenser which will be always below 1.
I know it would be boring for you, but could you not make a video about the basics of how to use a microscope for us who use it more as a hobby? It is not everyday I find a teacher that is so clear. Great work.
You'd LIKE me! I have my OWN stains, MY OWN scopes (compound AND dissection) and I frequently USE both. I also am goingto teach myself HOW to use immertion oil.
With oil immersion microscopy - do you add the oil directly to the fixed microscope slide or do you use a cover plate - or can you do it either way? Thanks
Wish you would take her class... Replay the vid. Put oil on top of fingernail, then place finger under lense, raise platform with finger until it touch lense. Keep raising platform until you can see cells in finger.
Question, why do you advise against Kimwipes for the lenses? My understanding was that they were made for such things, and many manufacturers even recommend them.
@@bioprofaz Thank you for the response! Everything I find online said Kimwipes are made for cleaning sensitive equipment and optics, regardless thanks to you I went ahead and bought some proper lens paper. The $5 is negligible when compared to the cost of a new objective. With that let me just say thank you for making these videos, they're highly informative and very well made. Just know your work is highly appreciated and I look forward to seeing what you have planned for the future.
@@evanbrown6923 Thanks for the kind words. I have to laugh when someone compliments me on my videos or asks me questions. I made these for my students as a pre-lab activity and never intended for anyone else to watch them. I resigned from teaching to start a business a few years ago. Its called BACK for Learning. Check out my website some time www.backforlearning.com/. I am still in the start-up stage of my business, but plan to expand with more kits, curriculum, books, and videos.
@@bioprofaz That's amazing! Just subscribed to the newsletter. You're a great professor, very knowledgeable and concise without seeming even the least bit pretentious like some professors can tend to be. I would love to be one of your students, though I'm looking for something a bit closer to High School/University level like what's presented in your videos. Your videos are perfect for people like me who have a desire to learn as much as possible, but without being enrolled in University for the rest of my life.(I'm a computer science major, who's never taken anything beyond High School Biology.) Biology is a passion of mine however, and I even have a small home laboratory for practicing various scientific disciplines. I've only just gotten a decent microscope so now I'm working on gathering various supplies. I would love if you could help provide some guidance.
I wish you were my mother or instructor because you're awesome! Anyway, I'm looking to purchase oil immersion for my microscope. (I'm considering to purchase a AmScope Microscope with digital cord to PC hook up and a magnification of 2500x .) Had I not watch this video ,man,I would be regretting using 100x with oil immersion. I didn't know you damage your objective with oil immersion. Thank you so much .
I'm going to buy a microscope ..Please any advice ?? in amazon I found many different of them and got confused !! want to use it for ecological studies.
Not in your class, but thank you very much for the guide to using oil immersion correctly before I destroyed one (or three) of my brand-new microscope's lenses!
Luckily, the lenses being parfocal, you can swing in the oil immersion lens when the specimen is in focus at 400X, and be close to having the field in focus at 1000X. Modern day microscopes allow for this because they are made very precisely, even if the individual objectives have been screwed in randomly in the holes on the nosepiece. This has not always been so, and if you were using an apochromatic objective and swung it into place, and you scratched it, you might be out a few thousand dollars. It is safer to place a drop of oil on the specimen when the object in question is in focus at 400 X and is centered in the field, and then lower the stage well below a potential scraping level, and swing and click the 100X lens into position - and then lower the oil immersion lens slowly while observing from the side of the microscope. When the surface of the oil immersion lens makes contact with the oil, there will be a flash of light. The lens will then have become properly immersed, and only the fine adjustment will be needed for focusing. Scraping and scratching of the turret lens will have been avoided. At that juncture, only the fine adjustment should be used - it should have built-in slippage if you go too far and compress the slide. Most microscopes now also have a lock ring on the outer rim of the coarse adjustment. When the oil immersion lens is in focus, rotate the lock-ring until it is snug, and the coarse adjustment, if mistakenly used, will not be able to lower the body of the microscope and objectives any farther than the locked position, again avoiding the error of cracking a slide or coverslip by using the coarse adjustment when within focusing distance with the oil immersion lens. It might be useful to adjust all microscopes in the lab beforehand to take advantage of this feature and prevent damage to slides or oil immersion lenses. The focal length of the 100x lens is etched on the barrel of the lens - it is usually in the range of 1.7mm or so. However, in actuality, it is smaller than the calculated range, which itself is based on a formula which assumes that the objective has only one lens; in actuality, it has several, and the true focal distance is smaller than the rated distance. If you have to turn the fine adjustment more than a fraction of a millimeter in the downward direction and your object has not come into focus, it is time to back off and start over.
bioprofaz MY 40X lens had touched the oil before I watched this video. Now what should I do to clean it? It's was a "Olympus Optical Co." immersion oil. Is xlene suitable for cleaning?
I believe she says: you focus the condenser. When the loop comes into focus, then the condenser is in the right place. After this step, you have to refocus the slide with the eyepiece adjustment knob.
if the oil gets on the other lenses, the damage isn't permanent if you send it to a professional to clean it. this is expensive though and it would probably be cheaper and easier to buy a new one each time which is also very expensive, so just dont get it on the other lenses
![Lp. Сердце Вселенной #30 ДВА В ОДНОМ [Один человек]• Майнкрафт](http://i.ytimg.com/vi/nrXy15orKhY/mqdefault.jpg)
This is one of the most thorough and helpful videos I've ever seen on UA-cam. I don't know who this woman is, but she is an amazing teacher.
This was very informative and clear! I am a college student taking microbiology and this helped make things more clear to me than reading from the manual. Thank you for this! :)
Whoever is in her class is lucky as "heck" :p
Lovely scientist/teacher/lady & very informative *and clear* video! Thank you pro.
I like how your shirts are the color of Saffranin and Crystal Violet. Nice Touch! :-)
Kaley H wut?
theyre stains used for dying microbes
Warlita ibrahim
BWES KUM.ZAMBO SUR
@@ToltAztec cv
nice observation
Love this. You're an amazingly thorough professor! Thank you for publicly posting this for all to watch!
Her students are lucky. Many teachers are not that dedicated and therefore you may see their students working in an institution or company while they cannot even use a microscope properly!
I'm in HOSA and using a microscope with oil immersion is one of the skills we have to learn. I found this really helpful to visualize the procedure. Thank you!!
Lol I like her "Do it or get out" approach lmao. We need to get this old school training back 😂😂😂. Easy video to follow.
Thank you dearly for this video. It is 10 years later but you are teaching me now. I am on a mission to help our aquarium industry in South Africa, and your video contributes to it. Much love and thanks.
I'm not even a student but I watched the whole thing.
I really appreciate your sharing of the little trick about focusing the light condenser, you save my day, thank you so much!!
4Carolina Kroger
Yes to learn how to use mashine it is very useful to us znd we learn about where the oil put up and how the mashine clean.
Very useful in xhiwing each part of mashine
What a great lady. Thanks for doing this. Our teachers for this particular subject are very organised and helpful in general(hence the link to your video), but there are so many of our teachers who are not. Really chuffed and grateful that you cared enough to make this, and that you are able to demonstrate that you know about what you are teaching, and that you care about your students. Whilst the lecture at the beginning might not be important for those outside of your class, for me it demonstrates that you communicate, outline expectations, provide information and opportunities to master skills. I even bet all of your assessments come complete with marking schedules and correct answers that you use to mark them with, and you probably provide feedback. This is a novelty at our university apparently despite the HUGE EXPENSE. Anyway... thank you :) Do you teach vet clinic practice at all?
Excellent lecture, thank you for posting! I've looked at other youtube videos on this subject and they tend to raise more questions than answers.
DONT FUCKING GO TO 40 GUYS!!!
Thank you for the tutorial. I recently purchased a microscope, but it came with no documentation, so your video helped to give me a lot of needed information. I appreciate you taking the time to do this.
Hey I have a quesstion and since you're the first comment can you answer a question for me? in 9:22 She said dabbing it with a can wipe or something like that. Did i mishear? If so what kind of wipe do you have to dab it with?
Hi, it's a Kim Wipe, which is essentially just a lintless tissue.
thanks
Thanks for tutyrian online. Can improve to individual.
Thank you so much for the details and the clear instructions how to use microscope to find the bacteria, fast . And thanks for your encouragement not to frustrated. But try hard , and to draw a circle, because the bacteria are so small, can’t be seen by naked eyes. And remember to put immersion oil only with 100x and lean focusing with fine knob.. you are amazi. I appreciate your explanation .
Awesome! Good luck with your lab!
This is a highly detailed demonstration for using a microscope in a perfect way. I am a chemist and I have never used light microscopes ever in my life but in my research I need to use them a lot and a lot.. I have been searching videos like this on youtube to get familiar with the proper working of the microscope. I am really thankful to the instructor of this video for uploading this nice video.. Cheers!!!!! and Thanks a lot !!! :) :)
this addressed a lot of the problems I was experiencing in class. Thank you for your videos!
Just received my new microscope and found this video...the instructions with my Swift microscope are very basic and your video has helped me understand how to use all the components and accessories. Excellently informative video - thank you :o)
glad the advice in the video is explained in no uncertain terms. important to be very clear with these fundamentals, which are often difficult for students to grasp, myself included. microbiology is hard and it pays to be meticulous and really consider as many aspects of your technique as possible. you might think people are just being anal but, when you realise why, it usually makes sense.
Very useful! I'm using a microscope at home with my kids, and even though I have some experience in microbiology, you gave some great pointers! Thank you
Very thorough. Extremely helpful. Thank you for uploading!
This was helpful! I’m in microbiology at the moment and had a little trouble working the oil lens. This made it more clear on what to do if I lost the focus.
Debora Hernandez I’m glad this was able to help you. Good luck in your class!
Ut must be fucost to use maschine .
Thank YOU! Watching your demonstration made the procedure easier. Now, all I have to do is retain and recall the process.
Really good explanations and tips. I did Biology in my undergrad but I wish that you were my lecturer back then. Thanks
This is one of the clear and detail instruction to use microscope correctly.
Thank you. I am not a biologist or a microbiologist but I am fascinated with the subject. I purchased a very nice Omax digital microscope and your videos are extremely helpful for the self learner.
Can I purchase same online?
I have such a problem with oil immersion! My professor won't help us focus it anymore, so I'm trying to learn on my own! I can find it on every objective but oil immersion. Trying to find out what I'm doing wrong. Thank you.
I thank you very much for teaching us how to do it. We have very stupid lecturers, very stupid demonstrators and they never showed us how to find a bacteria and how to do the the oil immersion. please keep posting videos like this.
Great video! I'm taking Microbiology now and watch the videos before going to lab. Very helpful! Thank you for posting:)
Thanks for the video! I will now be well prepared for my lab tomorrow :)
Best description teacher 👍🏻 thank you very much, indeed.
This is exactly what I needed for me and my daughters to learn how to use the 100x objective lense and oil immersion. This only thing left out is whether to use oil/b or oil/a.
A very special thank you for this video and also for the gram stain video. Invaluable resource for a beginner student. Thank you .
Very good presentation. Wish you had been my Micro teacher! Love the details.
you are the kind of teacher who makes understanding possible
Thank you! I'm not in your class, but this is a great tutorial!!!
Thank you!
Balakrishnan
balazoo.rsh@rediffmail.com
Nice information
Thank you for showing it.
Great teacher
much aloha! I have a skills test and this is my first time using a microscope this semester! This made me a lot more confident and comfortable! Mahalo from Hawaii ^^
Thank you so much! I am studying without classes which makes life difficult sometimes. This was very helpful!
Wish i had an instructor who can give directions as clear as her.
So again the bacteria is facing up and the labeled with sharpie is facing down while viewing under oil immersion, correct?
Oh! This tutorial is brilliant. Thank you.👍🏼
Her students are so lucky to have a teacher that great!
Great job with the video!
She explained it very well, but I would still be afraid to have her as a professor because I'm a bit slow, the beauty of videos is that you can rewind them lol
same
I'm so glad I watched this before buying replacement objectives... it's working now! :D
That is very cool!
In wacthing the machen is the guide to learn on how to clean and how do we use it the maschine
Thank you so much!! This is very helpful. Now I'm ready for practical test tomorrow :)
Super helpful videos for Micro! Thank you!
Thanks you saved me a class, I can do this at my house on my own time. well done
One of the best videos I’ve watched helped me pass ❤
How did you know that I am in microbiology??? That's why I am watching this! thanks for the video it was very informative
Superb lesson, many thanks.
Thank you so much!!! This really helps a lot for my practical exam !!
Thank you! Very instructive and thorough!
thank you for this explanation. very helpful
LOVE LOVE LOVE...!!! from a teacher who has no idea how we didn't destroy every single microscope we had in college. Master teacher, Thank You Dear = WOW
Thank you my grand father who is a scienceist got my a microskopt like this for my 12 birth day
(augast 20 2012) and i just set it up and he and I didnot now how to applie the oil
and now we do thank you very very much.
I wish my professor helped me like this video did. I can't wait to go to lab and try it. I only spent 2 freakin hours looking at slides without seeing anything! And my professors tip "walk away and come back to it, its there, you just aren't looking good enough" seriously?!
BEST tutorial I'm seen so far on youtube, thx
Wonderfully detailed overview! Brava!
Thank you so much. I feel like a pro after watching this video.
Aw, I’m so glad!
In watching how to clean each part and how to use its so very useful to both msle and female.
I usually have trouble finding bacteria. Do you think you could show us what to look for at the main objectives, 4x,10x, 40x, and 100x, and what exactly it looks like under the microscope? I’ve been told to look for grains of sand? If there is a certain way to prepare the slide, could you also include that? This would seriously be a great help!
Thank you for this lecture. I found it really useful.
Well done !
Thank's for your trick about the right condenser distance, I will use it
and thank's again for the oil cleaning demonstration
I kiss you
Philippe
I had 3 Biologies in Ontario Collegiate Studies of CANADA!! My 1st MicroScope Lab Instrument was in Grade 10 Biology,then Grade 11 Biology & Finally Grade 13 Gold Honourary Collegiate ,Ontario,Sciences.Although I was already familiar with with my Laboratory $300.00+Taxes Edmund Scientific 3 Achromatic-MFg Multi-Coated Objective Instrument, I only got my SUPERB-Lab KONUS ACADEMY with Laboratory with "Oil Immersion in May of 2011! Today,Ontario,CANADA Biologies are Grades,10,11,12.Grade13removed!!
I been watching many tutorials about how to use the oil immersion objective but no one (not even at my school) put oil between the condenser and the slide, i learned about this some time ago, you need to do this to have maximum resolution and get a serious benefit about using the oil immersion objective, if you not do this you are going to be limited by the numeric aperture of the condenser which will be always below 1.
is oil recommended for all items to be examined with 100x...even if they move?
thanks
I know it would be boring for you, but could you not make a video about the basics of how to use a microscope for us who use it more as a hobby? It is not everyday I find a teacher that is so clear. Great work.
You'd LIKE me! I have my OWN stains, MY OWN scopes (compound AND dissection) and I frequently USE both. I also am goingto teach myself HOW to use immertion oil.
With oil immersion microscopy - do you add the oil directly to the fixed microscope slide or do you use a cover plate - or can you do it either way? Thanks
Wish you would take her class... Replay the vid.
Put oil on top of fingernail, then place finger under lense, raise platform with finger until it touch lense. Keep raising platform until you can see cells in finger.
@@markcarter6333 Tf u talkin bout
That was nice & helpful..thanks a lot...
Can you use the 100 x on multiple slides during one period of testing or do you have to keep cleaning the lens and reapplying oil after every slide
Great video prep but i dont have a scholarship but I just bought a microscope, and I learned quite a bit just now..
Question, why do you advise against Kimwipes for the lenses? My understanding was that they were made for such things, and many manufacturers even recommend them.
We used them because they are designed for lenses, and we wanted to do everything we could to keep them from getting scratched.
@@bioprofaz Thank you for the response! Everything I find online said Kimwipes are made for cleaning sensitive equipment and optics, regardless thanks to you I went ahead and bought some proper lens paper. The $5 is negligible when compared to the cost of a new objective. With that let me just say thank you for making these videos, they're highly informative and very well made. Just know your work is highly appreciated and I look forward to seeing what you have planned for the future.
@@evanbrown6923 Thanks for the kind words. I have to laugh when someone compliments me on my videos or asks me questions. I made these for my students as a pre-lab activity and never intended for anyone else to watch them. I resigned from teaching to start a business a few years ago. Its called BACK for Learning. Check out my website some time www.backforlearning.com/. I am still in the start-up stage of my business, but plan to expand with more kits, curriculum, books, and videos.
@@bioprofaz That's amazing! Just subscribed to the newsletter. You're a great professor, very knowledgeable and concise without seeming even the least bit pretentious like some professors can tend to be. I would love to be one of your students, though I'm looking for something a bit closer to High School/University level like what's presented in your videos. Your videos are perfect for people like me who have a desire to learn as much as possible, but without being enrolled in University for the rest of my life.(I'm a computer science major, who's never taken anything beyond High School Biology.) Biology is a passion of mine however, and I even have a small home laboratory for practicing various scientific disciplines. I've only just gotten a decent microscope so now I'm working on gathering various supplies. I would love if you could help provide some guidance.
@@evanbrown6923 I am so jealous! I want a home lab. That is very cool! Yes, of course, I will give any guidance I can. I miss being in the lab.
Awesome vid. Very helpful. Thanks!
I wish you were my mother or instructor because you're awesome! Anyway, I'm looking to purchase oil immersion for my microscope. (I'm considering to purchase a AmScope Microscope with digital cord to PC hook up and a magnification of 2500x .) Had I not watch this video ,man,I would be regretting using 100x with oil immersion. I didn't know you damage your objective with oil immersion. Thank you so much .
I'm going to buy a microscope ..Please any advice ?? in amazon I found many different of them and got confused !! want to use it for ecological studies.
Not in your class, but thank you very much for the guide to using oil immersion correctly before I destroyed one (or three) of my brand-new microscope's lenses!
Luckily, the lenses being parfocal, you can swing in the oil immersion lens when the specimen is in focus at 400X, and be close to having the field in focus at 1000X. Modern day microscopes allow for this because they are made very precisely, even if the individual objectives have been screwed in randomly in the holes on the nosepiece. This has not always been so, and if you were using an apochromatic objective and swung it into place, and you scratched it, you might be out a few thousand dollars.
It is safer to place a drop of oil on the specimen when the object in question is in focus at 400 X and is centered in the field, and then lower the stage well below a potential scraping level, and swing and click the 100X lens into position - and then lower the oil immersion lens slowly while observing from the side of the microscope. When the surface of the oil immersion lens makes contact with the oil, there will be a flash of light. The lens will then have become properly immersed, and only the fine adjustment will be needed for focusing. Scraping and scratching of the turret lens will have been avoided. At that juncture, only the fine adjustment should be used - it should have built-in slippage if you go too far and compress the slide.
Most microscopes now also have a lock ring on the outer rim of the coarse adjustment. When the oil immersion lens is in focus, rotate the lock-ring until it is snug, and the coarse adjustment, if mistakenly used, will not be able to lower the body of the microscope and objectives any farther than the locked position, again avoiding the error of cracking a slide or coverslip by using the coarse adjustment when within focusing distance with the oil immersion lens. It might be useful to adjust all microscopes in the lab beforehand to take advantage of this feature and prevent damage to slides or oil immersion lenses.
The focal length of the 100x lens is etched on the barrel of the lens - it is usually in the range of 1.7mm or so. However, in actuality, it is smaller than the calculated range, which itself is based on a formula which assumes that the objective has only one lens; in actuality, it has several, and the true focal distance is smaller than the rated distance. If you have to turn the fine adjustment more than a fraction of a millimeter in the downward direction and your object has not come into focus, it is time to back off and start over.
Thank you for the loop trick! I had no idea!
This is really really helpful! Thank you for this video...i look forward for more :)
I have never used any micrioscope, but after such a good tutorial i feel like I'm ready to use oil immersion lenses right away😁
You look like a teacher that i wouldnt like but good info!! Lol
part of compound microspe
And I'm sure she's glad you're not her student, as you make such stupid comments.
Great Video...Very informative
What microscope is that? Thanks.
I have not worked in the classroom for about 4 years. I do not remember.
You are brilliant maaam
Thank you Ma'am! your the best!
bioprofaz MY 40X lens had touched the oil before I watched this video. Now what should I do to clean it? It's was a "Olympus Optical Co." immersion oil. Is xlene suitable for cleaning?
Sorry to hear that :\ so what did you do?
i didnt get the thing what you where doing in the beginning with the focus on the loop, what exactly do i adjust ) my english is not that good )
I believe she says: you focus the condenser. When the loop comes into focus, then the condenser is in the right place. After this step, you have to refocus the slide with the eyepiece adjustment knob.
Thank you very much Madam.
Please do videos like these
is that simplified Koehler ilumination? (3:48)
I never knew of the looo thing, thank you so much
if the oil gets on the other lenses, the damage isn't permanent if you send it to a professional to clean it. this is expensive though and it would probably be cheaper and easier to buy a new one each time which is also very expensive, so just dont get it on the other lenses
Wont the oil be romved using 90% ethanol and lens paper to wipe it off? Im kinda scared of practicals now
Fantastic information!
Best believe I won't be looking in my partner's microscope! Thanks, Teach!
why using the oil imersion in the center of the slide when use the 100x lens
Thank you, this was very helpful!
Very nice teacher
Thank you so much! you are wonderful.
God bless u thank wish urcould b my teacher
You’re amazing thanks