Media Prep
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- Опубліковано 13 гру 2012
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In order to grow, cells need very specific environmental conditions such as food, energy, proper temperature and humidity. When growing cells in the lab, we have to create these conditions using culture media.
Solid culture media is a mixture of agar and nutrients poured into Petri dishes. We can transfer cells to the Petri dishes where they will grow with proper incubation.
Sterile technique is required to create culture media so you don't have unwanted microorganisms growing in your culture.
In this video, we'll demonstrate the steps for preparing Brain Heart Infusion Agar. It's a general-purpose media used for a variety of bacterial and fungal species including pathogens like Streptococcus and Pneumococcus.
This is inaccurate. There’s not a middle aged lady yelling at you and complaining for you to go faster
Lmao
Hhahahah Amen my brother...
😂
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@Raksham Tiwari (B18BB028)
studyscience.rahulpagar@gmail.com
I love how science is always clean and organized. Btw, glass material looks better than plastic material. 🤩
Glass is incredible. Yet fragile.
Wow. Makes me wish i had 100% sterile conditions when it comes to laboratory work. But the budget isnt there. Thanks! For the insperation and observations.
In this way I prepare these plate in my work.Good explation to show my students👏😊
Una observación. Teniendo la CFL detrás de la operadora no había necesidad de vaciar el medio a la intemperie, pudiendo caer cualquier tipo de contaminante proveniente del aire, el mesón de trabajo, los guantes etc. No se protegió bien el producto estéril. Ahí hubo un error en el plaqueo.
she poured the media into petri dishes in open environment...should be done in laminar or other aspectic conditions
Lighting a bunsen burner does a good job, the convection current actually sterilizes the surrounding area.... obviously when the wind is still.
I used to do that and things worked fine. That said, I did see a lot of other people do poorly because it's not as forgiving for lack of attention.
Yeah you say correct technique to pour the media in dish if you have no laminar air flow device you complete your technique between the two burners....
Hence the desired bacteria we give from dish
I was wondering
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@@HaydenHatTrick would an alcohol burner work too?
Thank you so much this is very help for my Lab Practical
This was so helpful. Thank you so much!
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@@RahulPagar 111
Wonderful do love this
Superb work 👍
Great job I'm also student I'm MLT it's very best for me thanks alot ❣️
What a video mam love from India😍love😍😍
like it very nice and easy work 🌸
thats some fancy equipment...ill use my range top and a spoon
I like this video keep going 🤠 greeting from Morocco
I feel like this makes it look more complicated than usual. Of course I make media for college teaching classes that don't need the exact measurements and sterility of a research lab. If your going to go the way of making it super precise, why not go all the way and use a vent hood to pour the media and create a sterile environment. Plates also shouldn't be opened up so much when pouring as the plate should have come in sterile beforehand and opening them that much will allow microbes in the air to land in the plate easier.
But I will say this is great for giving my friends an idea of what I do all day because it's hard to explain without being able to show them.
can you tell me more easy way to make media? I'll have class soon.
Why not pour it in a 0% oxygen capsule. Nice comment. But you can only do so much with you'r resources, and the the holes of your result "might" not sway too much. You have a good point though. Ver good.
@@soengchatvichea4417 it's rarely easy. But if you work with bacteria, 90% steril is the only credible regards to te experiments.
hey there i am also feels the same i want some discussion about this work can we ?
Thanks for this video...
thank you so much it was very useful
interesting the advice on avoiding condensation in the petri dishes
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Tq sister... It is very useful 🙏
culture media preparation best technicians
God I wish all labs were this clean. Smh.
great vedio very helping and innovative please
Do we need to seal each of the agar plates with parafilm before keeping them in refrigerator?
"Make sure the heat is turned oawww"
Is she saying on or off?
Off
@@1020percent for what?
off
Thanks so much🌼
Very helpful video :D
thanks for help but somebody tell me more details
Why are agar plates (with micro-organisms on) only incubated for between 24 and 48 hours?
YummiestOrphan its the time required for bacteria and fungi respectively to grow....
opening the petri dishes widely will cause contamination. The procedure did not perform aseptic technique during the dispensing.
roche mahinay that's right
Yes we can perform asceptic condesation.
roche mahinay
You are right , I am student in second year applied biology and we don’t open the Petri dish widely , to avoid contamination!
Yeah.. atleast use a burner or laminar air flow
Dayyyym how much did your scales cost? I'd love a pair of those in my lab!
Use a kitchen scale. That was overkill.
Thank you very much 💯
good job
Would you please tell us :
The aluminum foil is applied to
the flask firmly or loosely ?
Can we prevent the water loss during autoclave process
or about what percent water loss
during autoclave process will
not effect final test results.
@@owenwhitman6616 Thank you very much for your instruction.
Agar media is costly ...which other media can be used
Thanks
it very useful to students
Amazing 💕
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In these comments, lots of people saying "ah, not the bench".
I just light a bunsen burner and work within a half meter of it. A bunsen's convection current works really well for even a meter radius around it.
@Nipha Ahtlantashah Based on your other comments I'm going to go ahead and say you've never even been in a lab :/
@Nipha Ahtlantashah .... its AGAR!!!! I'll prepare it in the turboincabulator from now on.
Could you show us algae analayes please
how do i know that how many plates are required for the pouring of agar?
It depends on how many plates you want to make and volume of media you have prepped. Each plate requires approx 20mL media. So 100mL media can make 5 plates
Why for days the tubes are filled to the end and a vacuum is required between the pads and the solution when sterilizing in the autoclave
thank you
Woaw super intéressant
Thank you so much
May I know what are those materials that you used in this procedure? Thank youuuu
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I have to use a particular bacterial strain in removal of a particular chemical. Shall after putting bacteria and flask in a chemical,How can I measure the quantity of bacteria?
5. The method I applied is putting a chemical and bacteria in a flask at a certain temp near about 25 C-30C?
In a water bath shaker and rotate it for 50-180 minutes and after that check, how much chemical is removed by this bacteria.
My question is, is this temp and rotation , destroyed the bacteria or it is Normal , we can do it.
why is it necessary to autoclave media along with magnetic stirer? Heat will affect it's property. moreover, not necessary to restire media after autoclaving.
The stirring probably helps to cool the agar down. Ouring agr when it's too hot will cause condensation to form inside the petri dishes.
hi ravi, autoclaving is done so as to remove any contamination from the media as well as all the other material that is being used.
no it does not affect aga properties ans stirring agar is necessary because agar tenda to soldify at the 30 to 40°c ..so if we do not stirr it it will soldify and settle down and we wont be able to get solidfied agar plates
for pouring purpose use laminar air flow. not in normal desk.
or a bunsen burner does just as good when you do have to do it on a bench top.
Pls help me my agar boils over in the pressure cooker whats wrong im using agar malt extract and honey fyi for growing mushrooms
Why we use deionized water instead of distilled water?
Can it germinate Orchid seeds
Bukankah penuangan media tidak boleh dibuka terlalu lebar?
For how long can Agar-prepped petri dishes be stored?
You can use upto 10 days atleast. After that, the media starts getting dry.
Usually a month if refrigerated.
Do animal cell culture media do the same thing?
I love it
why don't you heat when stirring the media?
Someone plz give me the experiment of this prac " media prep"
so good
tq so much
Hello! How many hours does it take to solidify?
zyreneium not hours, but 10-15 mins, also depends on agar concentration
And does she prepare Petri dishes outdoors without at least pouring near the flame of the Bunsen burner?! I don't like to prepare my plates outside the laminar flow hood... When I finish pouring the medium, I let them solidify with the hood closed and the UV turned on to avoid maximum contamination... When I prepare outdoors, I light the Bunsen burner and do everything there in the burner to minimize the risk of contamination, and detail, with the smallest possible opening of the plate. The way she did, it contaminated everything. 🤷♂️
Is agar compulsory could we prepare tissue culture at home??
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at last where did she store them n y using plastic bag
how to sterilize lab equipment ,when starts of time ?we starts up to 121 degree c?or after degree c reach 121 degree c ?can u send response ?
Why didn’t you used sterile conditions to pour the media? What if it gets contaminated?
please what is the size of the petri dish?
Life saving
How to prevent moisture after directly pouring warm media and covering the lid?
don't cover the plate immediately
Keep the petridish in the LAF and pour the media don't close the petridish. Close the front door of the LAF and turn on the UV light ( not necessory, if done it will prevent contaminations)wait till the media gets solidified then turn off the UV, sterilize your hands using sanitizers and work on.
Once work is completed seal your petri dish using paraffin flim and keep it in the Co2 incubator upside-down ..
This process will avoide moisture as well as contaminations
Let's for a second imagine a hypothetical scenario in which a person doesn't have a magnetic stirring plate or an autoclave. Are there some viable lower tech solutions?
Magnetic stirrer is not really necessary. Instead of autoclave you could also use pressure cooker. While preparing media I have never used magnetic stirrer.
I know this post is old, but I’ll answer it anyway. When making the solution, you can dissolve it in a very clean saucepan/pot, until the solution is completely melted and mixed together. Use a whisk or something similar to mix it. Then you can put the mixture in a glass flask (preferably borosilicate glass) with a polypropylene lid (or some other plastic that is heat resistant). Then you can put said flask, with lid lightly screwed on, in a pressure cooker; preferably one that can reach 15 PSI. Cook for maybe 20 - 30 minutes, and tighten lid properly soon after taking the flask out of the pressure cooker. If you tighten the lid too much before pressure cooking, you risk the bottle exploding because of the pressure buildup inside it.
@@Lemonz1989 This is some good advice! Thank you! Luckily, I have since been able to prepare some agar plates. As for my project.. Overall, it didn't go quite as planned. But for a time, I was able to keep some mycelium for medicinal mushrooms alive!! Unfortunately, the petri dishes were still only the easy part. :)
Please use LAF while pouring the media in the petridish to avoid contaminations
what is LAF?
or just simply alcohol lamp is enough.
I love how you can be of all possible thanks for breathren work smart goals you need help ask
MANY THANKS
Huge mistake there - not wearing a hair cap and having loose hair flap around. It might be ok if you're using a laminar flow cabinet, but if you aren't, it's totally wrong.
how can i prepare mrs agar
It's demonstration bro that's why she doesn't use laminar hood
@@sanjeevkumarnitrourkela6637 They don't care about that, they just want to be smarter and "better" than everyone else
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Bruh, if you're just growing some regular e. coli or something you don't need one. Most people don't do whatever you are doing with your agar. What lab do you work at?
Ma'am can I use this video
Good
What is the name media that you do? I mean... the media name, what is it?
nice
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How to convert cfu/g to cfu/ml
Wow
likely
good
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hi frend waht is the pouder nems add the wouter???
Agar
@@moh19931000 Thanks so mach may frend
ممكن ترجمة
You you N , some unipo students .
No aspetic conditions maintained.. y u even autoclaved media when u needed to pour it like this.. mixing with normal water mixing n pouring would hv given u same results
Nicer 👍🏻
Paduu Toqqq...
i was a lab technician for 4 years and now I want to be a dj
Anyone please tell me material required & process in proper written form. I have to solve my class work
👍
She's doing it in open environment, that might can cause contamination
LEZZZZ GAWWWWWW
We usually sterilize after pouring in petridishes.
What? How
@@neeruatishyavlogs521 i saw people quickly running the bunsen flame over the surface of the plate
im guessing thats how, never done it but yeah
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When you wanna teach things to people, teach the right way. We are discussing microbiology here, so I’m confused why is she dispensing the media out in the open. She didn’t even sterilize the media cylinder with heat. 🤔
AGARIO
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