Recombinant DNA Technology Explained For Beginners

Recombinant DNA technology is a series of techniques used to manipulate and isolate DNA segments of interest. In order to insert this isolate DNA into a cell for cloning, some type of vector is commonly used.
To create such a recombinant vector a bacteria containing a plasmid and a cell containing the DNA of interest is used. The plasmid from the bacteria is combined with the gene of interest that has been isolated.
This plasmid is a separate piece of circular DNA located in the bacteria and when we look closer at it we can see that it consists of complementary DNA strands just like normal DNA.
By using restriction enzymes to cut this circular DNA at two specific points, one can create two sticky ends that are complementary to the DNA of interest, allowing for easier binding of the gene of interest.
Then once the DNA strand of the gene of interest has bound to the two sticky ends of the plasmid, DNA ligase can be used to ensure proper binding.
What we are left with is a recombinant DNA plasmid which can be inserted back into a bacterial cell for cloning or into a mammalian cell for transfection.

[@biotechlucas4126]

What would you like me to cover next?

[@Salama7856]

Gene cloning please and thank you so much for the video it made it so clear.

[@biotechlucas4126]

@@Salama7856 Great suggestion! Thank you! Will add it to my videos-to-do list!

[@youshaaa69s21]

Watching this 5 hours before exam 💀💀🤫🧏‍♂️

[@biotechlucas4126]

Good luck!👊

[@farganakhanom2188]

Thanks a lot . Tomorrow is my exam your video helped. Love from Bangladesh 🇧🇩

[@biotechlucas4126]

That's great to hear! Good luck tomorrow!👍

[@nihalimtiaz8388]

I have an exam in 20 minutes

[@itsalavstory]

thank you. i've been reading this over and over for hours, but i understood it in one minute with your video

[@biotechlucas4126]

That's fantastic to hear and it's EXACTLY what I try to achieve in these videos so I'm really happy that I seem to have hit the mark!🎯

[@ssbb9793]

Understood easily in short period of time... Thank you 🙏🏻😊

[@biotechlucas4126]

That's fantastic! Thank you for taking the time to let me know it was helpful! :D

[@tanypacchioni]

Great explanation, thank you!

[@biotechlucas4126]

I'm happy to help!😇

[@azizrasulov4233]

Short and easy, thanks

[@biotechlucas4126]

Thank you! That is my comment-goal when I make videos!🙏🔥

[@ellaizajunenacilla7168]

Thank you, this really helps me with my report,☺️

[@biotechlucas4126]

I'm happy to hear that!🔥

[@singhnwaabvlogz3735]

thank you this was very helpfull

[@biotechlucas4126]

I'm really happy to hear that!

[@jannaibrahim7036]

so helpful thank you

[@biotechlucas4126]

I'm happy you found it helpful!

[@utkarshsingh4329]

Great explanation. Make more videos!

[@biotechlucas4126]

Thank you! Will do!🫡😇

[@EllyTaliesinBingle]

Very good video thank you

[@biotechlucas4126]

I'm happy you found it helpful!😇👍

[@user-jv5uj2ki3r]

Thanks from my heart ❤

[@biotechlucas4126]

I'm happy to help!😇👍

[@PrunellePrunelle-xm8lp]

Thanks
This will really help me for my exam❤

[@biotechlucas4126]

Great to hear! I hope it goes well!

[@biotechlucas4126]

Good luck! Please let me know how it goes!

[@naolmengesha4084]

great content

[@biotechlucas4126]

Thank you so much!🙏

[@MiniTour55]

Nice work

[@biotechlucas4126]

Thank you so much!🙏

[@user-kg7wj6yl1c]

this is nice bro👍🏻👍🏻

[@biotechlucas4126]

Thank you so much!♥️

[@user-lv8eb2ro9u]

❤

[@maaikeiseennaam8504]

strijder♥

[@zeeshannasir3558]

Podcast on Biotechnology
ua-cam.com/video/2O9EYjbZL4c/v-deo.htmlsi=aUra3zQSTJeZI6yI