Real time PCR

This pcr reaction lecture explains about real time pcr procedure. It explains the realtime pcr mechanism and uses in molecular diagnosis. Web-shomusbiology.com/
Download the study materials here-
shomusbiology.w...
A quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR), which is used to amplify and simultaneously quantify a targeted DNA molecule. For one or more specific sequences in a DNA sample, quantitative PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to DNA input or additional normalizing genes.
The procedure follows the general principle of polymerase chain reaction; its key feature is that the amplified DNA is detected as the reaction progresses in "real time". This is a new approach compared to standard PCR, where the product of the reaction is detected at its end. Two common methods for the detection of products in quantitative PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence to quantify messenger RNA (mRNA) and non-coding RNA in cells or tissues.
qPCR is the abbreviation used for quantitative PCR (real-time PCR).[1] Real-time reverse-transcription PCR is often denoted as: qRT-PCR[2][3][4] The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia.
Link- en.wikipedia.or...

[@whydizz]

How do people not understand what you're saying with your accent? You're English is much better than some of the other Indian lecturers. Thanks for taking the time to upload this!

[@CopperKettle]

I'm from Russia. I grew up listening BBC Radio and suchlike stuff.. In this lecture, some words are indeed hard to understand, sadly. But yes, it's better than other lectures by Indian people.

[@whatever9506]

your*

[@lokitekq7997]

Finally found my molecular biology plug on UA-cam.😁🔥🔥

[@shomusbiologyofficial]

You're welcome

[@adekunlemaryam1580]

Thumbs up. The way you explain this things is >>>>>>>>>

[@shomusbiologyofficial]

Thank you

[@hanaparos131]

Very informative and CLEAR. Thank you Mr. Bhattacharjee!

[@xHaniffax]

All your videos are very helpful! Thank you!

[@deepthirao5378]

Excellent presentation! It helped me a lot.. I didn't have any problem understanding his accent.

[@pragyasharma7986]

thanks for being such a versatile teacher

[@zdenkajp12]

Thank you so much , the explanation was complete and well delivered .

[@sivachaitanya3761]

wow...prachondo bhalo.. ei videota khub informative.. anek tekh kore bolchilen... ami M.S porchi IICB jadavpur the.. amadir QRT-PCR ache.. ami anek bar korlam ey technique.. kinthu basics chiney na.. ekhun jantham.. bcz of u..

[@mmaking8664]

onek technique amra kore thaki, kintu basics for granted niye nei

[@BilalAhmad-lf2np]

bro your lectures are really worth listening ..well done

[@samabayramiazar960]

super clear and comprehensive! thanks a lot

[@shomusbiologyofficial]

+sama bayramiazar you're welcome

[@tommasopusceddu1837]

At 24:00 you describe Scorpion primers not molecular beacons, molecular beacons are like scorpion primers but without the blocker and the primer. Molecular beacon probe will bind a specific DNA sequence and doing that the reporter and the quencher get away producing fluorescence.

[@kanchansharma8755]

thank u sir ur lessons are awesome

[@jesushernandez-gw2qj]

Thanks again for the great video. Pretty easy to understand.

[@shomusbiologyofficial]

Thank you

[@piko9311]

Thank you very much for sharing, it is very clear and informative. Congratulations for the good work!

[@gaurinerkar]

Very well explained. Thank you!

[@phearmetoo]

that is great! finally, I understand qPCR

[@bhu9984]

Amazing ... Made things much easier

[@misschaitali7942]

Thankyou sir for sharing your knowledge here. It helps us a lot.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@misschaitali7942]

Not general benefit i'm really thankful to you because many of your vdos helped me pass, sorry it helped me to get good marks in my final exam without using any book.

[@misschaitali7942]

You are really a good teacher. Thank you sir.

[@bluefate543]

This helps me a lot. Thank you !

[@shivrajSingh-nl2dr]

your video are very helpfull

[@clarkyyy28]

great video, thanks

[@temitopeisaacadelusi1264]

One word and I'll say Fantastico

[@mayukhroy5393]

this was really helpfull sir

[@shomusbiologyofficial]

You're welcome

[@sakiyamir5789]

plz explain Serial analysis of gene expression

[@stevenjuguna7944]

thank you bro....very helpfull

[@racooninabag]

nice one man this was so helpful

[@sosa222222]

very informative. many thanks

[@enasa9197]

your lesson amazing

[@pinalchaudhari6520]

very well understood. thank u.

[@SarahMMorsy]

really helpful ,thank you

[@shomusbiologyofficial]

You're welcome

[@likhithapeddi2041]

So helpful

[@laurenjoubert3100]

Fantastic instruction!

[@shomusbiologyofficial]

+Lauren Joubert thank you very much

[@shomusbiologyofficial]

+Lauren Joubert thank you so much

[@mrorvos]

thanks alot, very informative!

[@KrisJJohnson]

I love these videos, but they are always so quiet!

[@likhithapeddi2041]

Thank you

[@nitinchalla782]

Thank you very much, sir. This helped me a lot.

[@johnnysanyour6746]

Thank you !! very helpful

[@Tiyaraify]

Can someone tell me what the basic mechanism of the PCR technique is, please? Thanks in advance

[@evademe1]

well done !

[@lovelymahato296]

Thnk u sir🙏🏼

[@shomusbiologyofficial]

You're welcome

[@dvd2003]

Please don't compare Western blotting and PCR; they have different substrates and functions. Maybe you meant Northern blotting?

[@dhrubanandamukherjee583]

Sir western blot is for proteins and PCR is for DNA... how can we compare the sensitivities of these techniques??

[@aliajabbar1111]

than you very very very much

[@pranavsenthilvel2195]

Where can I view this powerpoint?

[@fatimakhan6130]

In starting it was very informative and clear but in the end it was very confusing I hardly get some points

[@akashmishra1285]

Yepp after the probe part.... in the flruroscence vs cycle number curves it was hard to get any points😰but initially it was pretty easy to understand.

[@khawarkhan264]

Sir will you please share notes of this lecture.

[@sepiabrown9204]

Thanks alot!

[@Mido55melebari]

thnx

[@b_kastle]

I appreciate the explanation but the wrong pronunciation got confusing at times.

[@anaisoceane3221]

hello!please how can i know wich technique do i have to use,our teacher gave us an abstract and she asked about which technique do we have to use in that and that experience , macroarrays,SAGE,qpcr.....

[@catadoption_pune]

Many inaccurate statements.
Ex: With reference to SYBRGreen he says : "it cannot be used in quantitative PCR" - umm..really?!

[@puggeele]

In qPCR you measure the quantity (initial concentration) of a SPECIFIC sequence of DNA. SYBRGreen isn't sequence specific, it just binds on the minor groove of any dsDNA (it gives you the initial concentration of all the DNA).

[@dhrubanandamukherjee583]

puggeele I guess the SYBR Green is attached with the TaqMan probe along with the quencher and a short sequence complementary to the centre of the target DNA

[@tommasopusceddu1837]

SYBRGreen is used in qPCR to mesure the initial concentration of targer dsDNA but is aspecific. So to improve the specificity we can use specific primers and analyze the melting curve

[@catadoption_pune]

@@tommasopusceddu1837 Awesome! Yes :D. I take it back, my comment is rubbish.

[@muhammetmustafaaydin1321]

add turkish subtitles pleaseeeeeeeee ..!

[@mhansibkhan2456]

Nice but understand is toup

[@ossielmais]

damn man work on ur accent otherwise ur the best!

[@baonguyenthien1971]

OMG please practice your accent, your pronunciation. You make an excellent teaching clips but i cant hear clearly so there are some important point i dont understand.

[@Aliaimene]

+Bao Nguyen Me too I understood every word he said, probably because I am not a native English speaker! Anyway, Very good and well explained video.

[@gardinenstangenhalte]

+Bao Nguyen Thien Well I got used to his accent very quickly and now after dozen of videos I don't have any problem in understanding him.

[@soumyabrataguha1833]

practise ur own accent u chinese son of a bitch

[@miliba]

horrible, but funny accent

[@HunterCivilianPlays]

+Miliba well thats not rude. Is he making fun of your accent? I don't think so!

[@thomazseixas1983]

good video, terrible accent!

[@vango911]

sorry don't understand you. accent is to heavy

[@gardinenstangenhalte]

+vango911 I got used to it. He is probably one of the best producer of biochemistry videos on UA-cam. He helped me a lot in mastering my medical exams.

[@bubu10435]

Very bad pronunciation, informative video though!

[@yourmomsucksinhell]

Thanks :)

[@yolintsai5459]

thank you