Great presentation. Thank you so much for such a detailed explanation and also your personal suggestions from your real experiments. It really helps me in setting up my experiments and knowing what to do.
Great presentation, thank you! But I wanna ask qPCR introduced in your video means the Reverse Transcription-qPCR, right? However, quantitative Real-Time PCR is also called qPCR. Actually, quantitative Real-Time PCR and Reverse Transcription- qPCR are different, which is because the former doesn't involve RNA extraction and reverse transcription, but only DNA amplification. Generally, Reverse Transcription-qPCR maybe more often be named as RT-qPCR or RT-PCR.
RT-qPCR or RT-PCR are not the same. RT-PCR is a conventional PCR but includes an RT step before the PCR, while RTqPCR is a real time (either quantitative or qualitative) PCR including an RT step either a one step reaction or a two step reaction, therefore when talking about real time, the standard abbreviation is q and not RT.
Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?
use this tool to design your qPCR primer www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool?src=google&gclid=Cj0KCQiA8aOeBhCWARIsANRFrQGM38dfc7LgVjoEkIVsjGq5-PZACD6BPEfMpYtlv1sBrioS_xZpK9saApwjEALw_wcB
We are Bioer Technology Company Limited that makes professional DNA/RNA purification machine, DNA/RNA purification kits, DNA/RNA PCR detection machine , consumable, antigen/antibody covid virus kit and DNA/RNA diagnostic kits manufacturer.
Thanks for sharing this wonderful presentation on qPCR. Detailed and very informative.
Great presentation. Thank you very much for detailed explanation.
In a word: Awesome. That was awesome.
Thank you so much for sharing such a nice and comprehensive video
EXCELLENT . VERY WELL EXPLLAINED. CLEARED ALL MY CONFUSION. THANKYOU SO MUCH
Great presentation, and very explanatory. Thank you!
Clearly explained....Thank you!
Thank you for your excellent videos!
Great presentation. Thank you so much for such a detailed explanation and also your personal suggestions from your real experiments. It really helps me in setting up my experiments and knowing what to do.
Very informative. Thank you
Thanks... Great tutorial
Great video...but qPCR is not limited to quantifying the cellular expression of mRNA.
Great presentation, thank you! But I wanna ask qPCR introduced in your video means the Reverse Transcription-qPCR, right? However, quantitative Real-Time PCR is also called qPCR. Actually, quantitative Real-Time PCR and Reverse Transcription- qPCR are different, which is because the former doesn't involve RNA extraction and reverse transcription, but only DNA amplification. Generally, Reverse Transcription-qPCR maybe more often be named as RT-qPCR or RT-PCR.
RT-qPCR or RT-PCR are not the same. RT-PCR is a conventional PCR but includes an RT step before the PCR, while RTqPCR is a real time (either quantitative or qualitative) PCR including an RT step either a one step reaction or a two step reaction, therefore when talking about real time, the standard abbreviation is q and not RT.
great ! thanks!
Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?
Excellent
Is it 1:10 working primer dilution right? Should it be ddH20 - 180 ul
i'm interested in the info but the vocal fry is making it hard to listen.
Excellent presentation, thank you! But all this explanation is about qRT-PCR and not qPCR. Am I right?
You’re a god !
We need to design a specific qPCR primers of the gene or we can also used the primers designed for common pcr?
use this tool to design your qPCR primer
www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool?src=google&gclid=Cj0KCQiA8aOeBhCWARIsANRFrQGM38dfc7LgVjoEkIVsjGq5-PZACD6BPEfMpYtlv1sBrioS_xZpK9saApwjEALw_wcB
@@ahmed-labThank you very much. I appreciate 🙏
14x 🤔🤔
We are Bioer Technology Company Limited that makes professional DNA/RNA purification machine, DNA/RNA purification kits, DNA/RNA PCR detection machine , consumable, antigen/antibody covid virus kit and DNA/RNA diagnostic kits manufacturer.