What is RT-PCR? (Real-Time PCR & Reverse Transcription PCR)

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  • Опубліковано 22 гру 2024

КОМЕНТАРІ • 80

  • @sidratulmontaha4939
    @sidratulmontaha4939 Рік тому +51

    i dont know why i am studying at a university WHEN THESE CHANNEL ARE HERE ON UA-cam . really ggood

  • @azca.
    @azca. 2 роки тому +44

    I'm suspecting this will be one of those channels that will be incredibly successful in years to come. Growth will be slow especially on UA-cam, but if you continue producing incredibly informative and quality explanations like this, I'm sure you will go far. You explained this concept perfectly. Thank you!

    • @YouTooBio
      @YouTooBio  2 роки тому +6

      Thank you for the lovely compliment! I'm glad my video was helpful.

  • @abineshanbazhagan6703
    @abineshanbazhagan6703 Рік тому +5

    Being new to PCR and stuff, I was really finding it confused , especially after going through the literature. But here? It's a wow, never I had come across of such an explanation which not just cleared my confusions but paved a way for some novel research ideas. Ton of thanks. Warm appreciations and looking for more of this kind. Thanks again. Good day.

  • @BustinStephen
    @BustinStephen Рік тому +6

    The MIQE guidelines published in Clinical Chemistry in 2009 clarify this issue: RT-PCR is reverse transcription PCR. Real-time quantitative PCR is qPCR.

  • @GabeThinks
    @GabeThinks 2 роки тому +6

    Incredibly clear, engaging, and informative video! I needed a quick refresher and I now am totally comfortable with the concept of RT-PCR and qPCR. Thank you for making this!

  • @monenga
    @monenga 2 роки тому +3

    Thank you so much! You are the best explainer ever. Plus, you have such great energy and demeanor, you are also fun and pleasant to watch and to listen to. I hope you are a teacher!

    • @YouTooBio
      @YouTooBio  2 роки тому +1

      Thanks! 😊 I am a teacher!

  •  6 місяців тому

    OMG.
    Loving your explanation (and thanks for it), the music, and the dynamic of the video.

  • @xxdeadqueenxx5715
    @xxdeadqueenxx5715 2 роки тому

    Thank you so much for this!! I needed to understand how exactly real time pcr worked for a case study I have to present in front of pathologists for school.

  • @malihaaneeqa2343
    @malihaaneeqa2343 Рік тому

    The best exaplanation so far

  • @AW-yb5qy
    @AW-yb5qy Рік тому

    Do you have a video explaining amplification techniques such as FRET, TaqMan, Molecular Beacon, and Scorpion Probe? HELP!

  • @mahdiachbar3756
    @mahdiachbar3756 2 роки тому +2

    Just WOW !
    Excellent explanation really !!
    Thank you❤️❤️

  • @falloutmodder
    @falloutmodder 5 місяців тому

    Thank you for this excellent video, very clear and helpful! Excellent help studying for my Microbiology final!

  • @David_Hogue
    @David_Hogue Рік тому

    I love how easy this makes it to understand

  • @prabalmajumder5179
    @prabalmajumder5179 2 роки тому +1

    You made it crystal clear mam ..thanks a lot . please make more explanatory vdos

  • @synapticsarah
    @synapticsarah Рік тому

    Amazing example of scientific communication!! Thank you from a fellow science communicator.

    • @YouTooBio
      @YouTooBio  Рік тому

      Always special to get a compliment from another science communicator!

  • @Aston_Tom
    @Aston_Tom Рік тому

    Sorry if I sound incorrect here, but through explaining Reverse Transcription PCR you explain how when you have regular DNA at the cDNA stage, does that imply you have left the Uracil in it, with not using RNAse H and Extending the DNA from the fragments to create a double stranded copy DNA of the original mRNA?

  • @Jesusandbible
    @Jesusandbible Рік тому

    the RT PCR Test used for Covid-19 - Though it is easy to trace the discovery of Reverse transcriptases to Howard Temin , and its independently isolation by David Baltimore in 1970, and it is also very easy to identify the inventor of underpinning PCR Test as Kary Mullis, I have yet to identify a scientist or group of them that take the credit for each of the RT PCR Test (real Time vs. Reverse Transcription). Do you know? Does anyone know?

  • @ΑδαμαντίαΨευτογκα

    great video! its a talent to explain so complicate things, so easy! Congrats!

  • @kaylabrandt2992
    @kaylabrandt2992 2 роки тому

    Thank you for such an informative and educational video! This helped me learn the difference in RT-PCR and PCR for my Virology exam tomorrow!

    • @YouTooBio
      @YouTooBio  2 роки тому

      Great! Glad it was helpful!

  • @alisasj2382
    @alisasj2382 Рік тому

    Thank you very much for the simple and concise explanation

  • @deresekia7344
    @deresekia7344 Рік тому

    Everything here is lovely explained, thanks!

  • @debjitbanerjee8512
    @debjitbanerjee8512 3 роки тому +5

    Please make more such amazing videos.

  • @dr.mousumidasgoswami5089
    @dr.mousumidasgoswami5089 2 роки тому +1

    Wonderful explanation

  • @alinaaleman4264
    @alinaaleman4264 3 роки тому +2

    Great video, easy to understand! thanks

  • @tommytruong4255
    @tommytruong4255 2 роки тому +1

    This is super helpful. Thank you so so much for doing this

  • @richardsarabi2064
    @richardsarabi2064 Рік тому

    You are not showing on your graph the number of copies. Like you said ten versus 10000 is not shown on the graph. I get the guicker plateau in fewer cycles and that of course means more copies for that sample, but it's not plotted on the y axis.

  • @elibenaron
    @elibenaron Рік тому

    underrated video

  • @kaylacraddock8670
    @kaylacraddock8670 Рік тому

    Helloo, so we know that DNA polymerase cannot use RNA as a template to build a complementary strand. What is the biological reason therefore? Is it due to structure or direction of activity?

  • @somoshreetaluqqder3706
    @somoshreetaluqqder3706 2 роки тому

    Extremely thankful.
    Wonderful explanation.

  • @laetitiaf.2671
    @laetitiaf.2671 9 місяців тому

    Thank you for the explanation. They are really clear

  • @bozhang6135
    @bozhang6135 9 місяців тому

    Amazing video! Keep up the great work.

  • @jennifersmith677
    @jennifersmith677 2 роки тому

    This was so helpful. Highly recommend!

  • @dereksniper
    @dereksniper 10 місяців тому

    good use of real life examples

  • @ayleenc.205
    @ayleenc.205 Рік тому

    So can you use primers for specific genes that code specific proteins and then use RT PCR in both its meanings to quantify how much there is of each mrna?

    • @anacarolina1578
      @anacarolina1578 7 місяців тому

      i guess so, because that's exactly what i'm doing in my masters degree haha

  • @tusarbehera6359
    @tusarbehera6359 Рік тому

    🎉🎉You are good a teacher

  • @aousshaheen3239
    @aousshaheen3239 Рік тому

    Very profitable video thank you ❤

  • @halilahnordin37
    @halilahnordin37 2 роки тому

    After complete reverse transcriptase and real time pcr,how we test the sample, validating it has sars cov 2?

    • @YouTooBio
      @YouTooBio  2 роки тому +1

      If there is PCR product after reverse-transcription PCR, then there was SARS-CoV-2 in the original sample. If there is no PCR product, then there was no SARS-CoV-2 in the original sample.

  • @saraabdulaziz3764
    @saraabdulaziz3764 2 роки тому

    Thank you so much for this!!

  • @josephakinkunmi
    @josephakinkunmi 9 місяців тому

    this was excellent. thank you.

  • @ranaelnady4128
    @ranaelnady4128 Рік тому

    AMAZING THANK U SO MUCH!

  • @ahmadhegazy7811
    @ahmadhegazy7811 3 роки тому

    can you recommend for me a book to understand the extraction of alkaloids or extraction in general?
    and thanks

    • @YouTooBio
      @YouTooBio  3 роки тому

      Interesting question! Unfortunately alkaloid extraction is outside my area of expertise, so I can't make specific recommendations. :(

    • @ahmadhegazy7811
      @ahmadhegazy7811 3 роки тому

      @@YouTooBio
      Ok.
      So, any recommendations about practical books in your field?

    • @YouTooBio
      @YouTooBio  3 роки тому +1

      @@ahmadhegazy7811 I can't recommend specific books in my field - we tend to learn techniques through direct mentorship from other grad students or postdocs in the lab. However, if you're looking for step-by-step video tutorials, definitely check out Jove www.jove.com/

    • @ahmadhegazy7811
      @ahmadhegazy7811 3 роки тому

      @@YouTooBio thanks 🖤

  • @beatrizsfsilva
    @beatrizsfsilva 2 роки тому

    Amazing content!

  • @amagaming2585
    @amagaming2585 2 роки тому

    shere material please

  • @Nooruddinsadiq
    @Nooruddinsadiq Рік тому

    Thanks ❤

  • @jusocampo
    @jusocampo 6 місяців тому

    great vid thank u!!

  • @christianutida1589
    @christianutida1589 2 роки тому

    thank you so much!

  • @sebastianbalaguera3017
    @sebastianbalaguera3017 24 дні тому

    Good video

  • @ManarAlAhmed-te5ww
    @ManarAlAhmed-te5ww 2 місяці тому

    She is so cool !!!

  • @sara97979
    @sara97979 2 роки тому

    awesome

  • @thesuperiorbench6307
    @thesuperiorbench6307 2 роки тому

    Cheers Jamie :D

  • @soosyr1996
    @soosyr1996 10 місяців тому

    I owe you :( super thank you

  • @tseringtashi7016
    @tseringtashi7016 Місяць тому

    Hi thank you for this wonderful video. I don't know if you get this a lot but for a second I thought you were the bumbling biochemistry youtuber just aged a little lol but I don't think you are haha.

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