Easy CRISPR/Cas9 plasmid cloning

[@nicholasmays4257]

this really helped me with a presentation I worked on!! thank you for making this so clear!

[@RahulPatharkar]

Glad it helped you!

[@NAA077]

Can you record a video how to insert this gRNA fragment within the plasmid between two location of a restriction enzymes? thanks

[@RahulPatharkar]

Golden Gate cloning which is shown in this video does insert a gRNA fragment into restriction sites. Please check the video carefully to see the restriction sites.

[@rohithnirmmals6305]

May I know, what tool was used in the video??

[@RahulPatharkar]

SnapGene

[@BBMCHCOVIDLAB]

How to design construct for gene knock in?

[@RahulPatharkar]

You just need to incorporate a repair template.