Thank you soooo much! This video is extremely helpful. Should mention that you have a nice voice and pronunciation, it's so important for a non-native speaker:)
Thank you so much. The way you explain by breaking everything down makes it so easy to understand. I hope you make more videos like this. Also, your way of articulation and slides are amazing and never bores me out.
Thanks a lot Jacob... .. . Even few of my Postdoc Colleagues were not able to make me understand it like the way you did... .. . Really appreciate it a lot
Thank you. Please make more videos on techniques used in the field of Biotechnology. The way you explain procedures and processes is very easy to understand and follow. You, sir, are amazing.
Great video man, my professor just isn't all there and no one in our course understood how to design a gRNA :/ With your Video I just get it now. Thanks for the clarity and especially the explanation how the algorithm works and how you could do it yourself. Such a great help thx again
I have been looking for a good source for gRNA design for several weeks. Only with this video, I could find the answer to almost all of my questions. Thanks a lot, Jacob. Great job. Would you please add a quick video on how to design gRNA for CRISPR/dCas9 system for gene expression regulation? Thanks
Amazing explanation! Please make more video related this topics! it's better than reading 1000 paper! Recently I am coming UA-cam to see your video! already subscribed and clicked bell icon
Comprehensive details, the Best method of explanation. I am gonna subscribe and like your channel to support your generous efforts. Thanks btw for the time and effort you put into this video.
Thank you so much for the excellent video! I have a few questions about guide RNA (gRNA) design that I hope you can help with. I'm working on a gene with three exons and two introns. The first two exons are in the 5' UTR, and the third exon is partially in the 3' UTR. When I try to design gRNAs for exon 1 and exon 2, I struggle to find optimal matches. Even when one meets criteria like GC content and self-complementarity, it still has mismatches, some of which are in the exons or CDS regions. Following your method from the video, I input the gene ID and found the exon blue blocks and intron lines. However, I couldn't identify suitable gRNAs for exon 1 and exon 2, while exon 3 displays many potential gRNAs. Since I’m still new to gRNA design, I'd really appreciate your guidance. Should I focus on designing separate gRNAs for exon 1 and exon 2, or should I concentrate on exon 3, given the better matches? Your advice would be incredibly helpful!
Hi, your video explained all the aspects clearly. I just want to know about the strand option there are"'+" and "_" signs in that column. Which one we should select? I am confused here.
I love your content, and absolutely appreciate the offer to check our gRNAs. I'm sure there's limits to how much time you can spare, but when it's most important, I will take you up on that offer.
Great video, thanks for taking the time to explain how it works, this is great help. I have one question, how should I proceed if I want to excise a potion of an intron and remove it completely? Thanks in advance.
Hi Jacob, I am currently undertaking a CRISPR.Cas9 experiment with Wheat for my masters degree and Chop-CHop isn't working for me neither Synthego - keep getting erros. You said in the video you would be happy to help , so just giving a long shot here in case you read this msg!
Thank you, the video is a great help; but what about the scaffold RNA? is it included in the plasmid in addition to Cas9 encoding gene? In this case, we do not design the plasmid, we purchase it from some company!
Hi why are there two complementary strands? I thought crispr was single guide strand. How will these attach to the genomic dna if they are attached to themselves?
i have just one question. How will I link Cas 9 protein, my gRNA, and those promoters to plasmid. if you can do a video on that, i will really appreciate
I read 200 papers to understand CRISPR knockout and it was almost impossible. This is the best explanation ever. Simple and efficient. Thanks!
such a great videos i read so many papers but nobody explained this clearly
I can’t believe someone can explain this so simply while being detailed! Thanks Elmer! 👍
I am usually skeptical of long videos, but this one explained everything I needed to know well and did not waste a single second. Thanks!
Thank you soooo much! This video is extremely helpful. Should mention that you have a nice voice and pronunciation, it's so important for a non-native speaker:)
The greatest explenation of CRISPR in UA-cam. Congratulations
I paid fees to people that confused me. In less than 30mins you explained everything. Thanks so much.
Probably the best video..... your efforts are really appreciated:-)
this video much more informative and clear than classical procedures, especially the chopchop part is very helpful. . Thanks a lot for sharing.
Thank you so much. The way you explain by breaking everything down makes it so easy to understand. I hope you make more videos like this. Also, your way of articulation and slides are amazing and never bores me out.
Thanks a lot Jacob... .. . Even few of my Postdoc Colleagues were not able to make me understand it like the way you did... .. . Really appreciate it a lot
Congratulations Jacob Elmer, I really like it. Super didactic. Surely one of the best video.
Very informative video. Clear all doubts about gRNA design.
Grateful for this video. It has been helpful. You are a good teacher.
i have never done CRISPER, after watching this great master lecture i am confident that I can do it very well in lab. very detailed practical steps.
Best tutorial on this topic, I've ever watched. You have another subscriber here. Many thanks!
Excelente trabajo explicando ❤ Muchísimas gracias!
So beginner friendly and helpful. Thank you so much! ♥️
This video was amazing, Jacob! thank you so much for sharing it!
OMG!!!! FINALLY, I GOT IT! This video is the best ever Jacob:) Thank you
Very informative especially for the beginners. Thank you for making this video!
Thank you. Please make more videos on techniques used in the field of Biotechnology. The way you explain procedures and processes is very easy to understand and follow. You, sir, are amazing.
very good video. Easy to understand such a difficult approach.
This is the best video I have found on this topic. Thank you.
Amazing video easy to understand and you have a really nice way of explaining ! Keep up the good work
More than a Perfect Video!!
thank you for making this video in such clear and practical way!
My first word after watching this video was WoW. Not usual for me.
It's just the perfect explanation. Thank you!
This is a fantastic explanation; thank you so much!
Utterly resourceful, definitely deserve more views and subscriptions!!
Btw, chopchop is constantly in use, priority 6-9 is common..
Thank you so much for the simplicity and clarity of the instructions
So nice presentation... Very informative & easily digestible material.. Much useful than other Videos.. Thanks a lot
Great video man, my professor just isn't all there and no one in our course understood how to design a gRNA :/ With your Video I just get it now. Thanks for the clarity and especially the explanation how the algorithm works and how you could do it yourself. Such a great help thx again
I have been looking for a good source for gRNA design for several weeks. Only with this video, I could find the answer to almost all of my questions. Thanks a lot, Jacob. Great job. Would you please add a quick video on how to design gRNA for CRISPR/dCas9 system for gene expression regulation? Thanks
Thanks so much! This was incredibly helpful and I appreciate all of the useful tidbits!
Dude, this is thy best CRISPR video on the net! tnx
Amazing explanation! Please make more video related this topics! it's better than reading 1000 paper!
Recently I am coming UA-cam to see your video!
already subscribed and clicked bell icon
Very useful contents.thanks dear
Thanks for the wonderful informative video :) .
the best video! thank you, please can you continue this video for the next step of CRISPR/cas9?
Thanks jacob. I loved how finely you explained everything. I was wondering if you make a video on crisprs mediated repair outcomes.
Sir, very well informative lecture for me I loved the way you explained.
Thanks for this very explicit video
Amazing explanation
Very informative lecture with details, thanks a lot!
cant thank you enough for making this video!
Awesome 🎉
Thankyou so much! Please keep making engineering videos
Perfect explanation thank you!
SIMPLY PERFECT
Really helpful. More video expected on CRISPR
thank u for simple but nice explanation, it helps me a lot
This was really helpful, thank you for making it!
I love your explanation..
Very useful and instructive
Comprehensive details, the Best method of explanation. I am gonna subscribe and like your channel to support your generous efforts. Thanks btw for the time and effort you put into this video.
sir u are amazing
I really loved the way u explained
u made my job much easier
plz make an video how do I clone a dna segment in to a vector in detail
Very nice dear sir
Thanks a lot for your explanation it helped a lot
Extremely helpful! Thanks a lot
This video is amazing
Thank you so much! very useful and clear explanation!
Thank you so much, you really explained so clear🙏
thanks a lot, it is extremely informative !
This is outstanding
Oh my God, you save my life. Thanks a lot!
Thanks thanks thanks for the help! You're a legend!
thanks for this tutorial.
awesome video!! thanks!!
This was very helpful
Thank you,, Please make a video on Analysis of ON and OFF target from NGS seq.. Cheers
thank you very much, it's very helpful!
This is great! Thanks!
great video indeed
Awesome Video thanks alooooooooot
Really nice tuto thanks !
Thank you!
much love
Genius!
Bravo!
Superb
Thank you so much for the excellent video! I have a few questions about guide RNA (gRNA) design that I hope you can help with. I'm working on a gene with three exons and two introns. The first two exons are in the 5' UTR, and the third exon is partially in the 3' UTR. When I try to design gRNAs for exon 1 and exon 2, I struggle to find optimal matches. Even when one meets criteria like GC content and self-complementarity, it still has mismatches, some of which are in the exons or CDS regions. Following your method from the video, I input the gene ID and found the exon blue blocks and intron lines. However, I couldn't identify suitable gRNAs for exon 1 and exon 2, while exon 3 displays many potential gRNAs. Since I’m still new to gRNA design, I'd really appreciate your guidance. Should I focus on designing separate gRNAs for exon 1 and exon 2, or should I concentrate on exon 3, given the better matches? Your advice would be incredibly helpful!
Hi, your video explained all the aspects clearly. I just want to know about the strand option there are"'+" and "_" signs in that column. Which one we should select? I am confused here.
Can select anyone. Both are right.
AMAZING THANK U SO MUCH
good work
Lovely
Dear, Greetings, You are awesome
I love your content, and absolutely appreciate the offer to check our gRNAs. I'm sure there's limits to how much time you can spare, but when it's most important, I will take you up on that offer.
Great video, thanks for taking the time to explain how it works, this is great help. I have one question, how should I proceed if I want to excise a potion of an intron and remove it completely? Thanks in advance.
Informative! Can you also make a video about knock-in design ? Thanks.
Te amo, gracias me salvaste
That was a great video. Thank you very much. How about if we want to Knock in a gene?!
Thanks. Do you have a Knock-In video?
Hi Jacob,
I am currently undertaking a CRISPR.Cas9 experiment with Wheat for my masters degree and Chop-CHop isn't working for me neither Synthego - keep getting erros. You said in the video you would be happy to help , so just giving a long shot here in case you read this msg!
Thank you, the video is a great help; but what about the scaffold RNA? is it included in the plasmid in addition to Cas9 encoding gene?
In this case, we do not design the plasmid, we purchase it from some company!
Excellent explanation.
Hi why are there two complementary strands? I thought crispr was single guide strand. How will these attach to the genomic dna if they are attached to themselves?
i have just one question. How will I link Cas 9 protein, my gRNA, and those promoters to plasmid. if you can do a video on that, i will really appreciate
thanks a lot,... so well explained , could you please explain more about NGG sequence ? thanks
basically its the PAM sequence and I think its used as a way to identify the difference between the gRNA and the sequence