Bacterial Smear Preparation

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  • Опубліковано 15 вер 2024

КОМЕНТАРІ • 53

  • @ericagovea9203
    @ericagovea9203 6 місяців тому +4

    Why aren’t you using any gloves ? Is it PPE not required ?

  • @DatChoklateGurl69
    @DatChoklateGurl69 8 років тому +37

    why didn't you have on gloves?

    • @iradukundasylvie1148
      @iradukundasylvie1148 6 років тому +2

      I guess it's not necessary

    • @cassiloca
      @cassiloca 5 років тому +1

      you don't need them

    • @kvnkvn9191
      @kvnkvn9191 5 років тому +4

      Not necessary because culture is created so no contamination chances in sile by hand

    • @ramkay9706
      @ramkay9706 5 років тому +9

      youre dealing with fire, glove is not necessary

    • @fluffandstuff6089
      @fluffandstuff6089 4 роки тому +1

      Could cause a burn if the glove melts on your hand

  • @LifeWithLonwabo
    @LifeWithLonwabo 2 роки тому +2

    why do we heat the mouth of the tube

  • @kenlordnavarette8734
    @kenlordnavarette8734 3 роки тому +4

    What do you think would be easier to prepare bacterial smear from, liquid or solid culture?

    • @abrarbhatti8978
      @abrarbhatti8978 Рік тому

      Send your whattsapp number

    • @_sadan981
      @_sadan981 11 місяців тому

      هل وجدت الجواب؟

    • @L4v4molly
      @L4v4molly 4 місяці тому

      It's easier to obtain colonies separately from solid media. It's also more likely to carry unnecessary parts from the liquid media.

  • @ayatosakamaki92
    @ayatosakamaki92 3 роки тому +2

    I'm confused , is the first drop of water compulsory???

    • @user-gd5wv8ls8l
      @user-gd5wv8ls8l Рік тому +1

      Water is required only if its a solid culture from a plate but not for broth cultures

    • @freefirebluezone
      @freefirebluezone 6 місяців тому

      ​@@user-gd5wv8ls8lthnku

  • @edidiongekpo
    @edidiongekpo 5 років тому +1

    Great video!

  • @yeppy013
    @yeppy013 6 років тому +1

    What is the purpose of air drying before heat fixing? Can't the heat fix do that as well as to dry the slide quicker?

    • @angelaasencion397
      @angelaasencion397 5 років тому +2

      The water will boil out and the bacteria will be destroyed.

    • @eclip9
      @eclip9 5 років тому +2

      Heat damage them so you need to be careful already you can dry it on the flame but with keeping a good distamce in order to prevent it from getting hot

    • @isiomawizzy3669
      @isiomawizzy3669 10 місяців тому

      Air drying preserves the morphology of the cell but heating distorts it.

  • @maryamadarbeh2323
    @maryamadarbeh2323 4 роки тому +1

    Why it’s important to air dry the smear before heat fixing?

    • @tayelorg8605
      @tayelorg8605 4 роки тому +6

      Hi, I'm a microbiology student in my second year. If the sample isn't dry first you are likely to boil the bacteria instantly and all your cells may lyse destroying them.

  • @sicelogemane9249
    @sicelogemane9249 4 роки тому

    Why the alcohol is used to clean the slide and not water?

  • @user-wk2gp3tu8h
    @user-wk2gp3tu8h 2 роки тому +1

    Thank you ❤️😊💯

  • @MohammedAyaanQureshi
    @MohammedAyaanQureshi 3 роки тому +1

    Thankyou. Doc

  • @althehore20
    @althehore20 3 роки тому +1

    Good ❤

  • @epic11introvert77
    @epic11introvert77 3 роки тому

    इस there bacteria in water and air will not affect it

  • @chandannishad4167
    @chandannishad4167 3 роки тому +1

    Vedasi mam ki aaesi ki tasi . 😁😁👍

  • @محمدشاكرفليححسن.2
    @محمدشاكرفليححسن.2 3 роки тому +1

    good

  • @lister2504
    @lister2504 3 роки тому

    Good video

  • @jesslynwong4666
    @jesslynwong4666 4 місяці тому

    NO GLOVES ?! OH NO

  • @beomnyang
    @beomnyang 2 роки тому

    good job susan

  • @noorjahan2246
    @noorjahan2246 3 роки тому

    What she took from test tube??

  • @zuluzero4659
    @zuluzero4659 5 років тому +4

    Where is ur lab coat where is your gloves?

    • @CynicalDuty
      @CynicalDuty 5 років тому +1

      chase the bag, don't worry about what i'm doing

    • @zuluzero4659
      @zuluzero4659 5 років тому

      @@CynicalDuty yes but your contaminating the results so thats wrong. Your also a threat to your health. I have got my youtube money hun x there is a protocol and h&s coshh for a reason if you work for any creditied lab or school and see you doing this, you will get reprimanded.

    • @CynicalDuty
      @CynicalDuty 5 років тому +2

      @@zuluzero4659 bust down, thotiana

    • @zuluzero4659
      @zuluzero4659 5 років тому

      @@CynicalDuty Uh, ayo, I been a bad b*tch, cockiana (Cockiana) / And I'm still winnin', Pacquiana (Pacquiana) / Percocets, popiana, killin' everybody beat / Thank you next, Ariana (Ariana)

    • @tayelorg8605
      @tayelorg8605 4 роки тому

      @@zuluzero4659 I'm not too sure which school you are referring to but at Curtin in Perth, in second-year Microbiology we don't use gloves. It is optional. As for the Lab coat yes it is standard PPE and we all need one. The results are not contaminated by bacteria on our hands nor can the bacteria on the stain infect us as it has been made bacteriostatic from use of the gram staining reagents. The bacteria on the slide are in too significant number to be contaminated by our own natural flora. Note also how they have been heat fixed first so our natural flora would just be washed off the slide if it was present.

  • @2009abody
    @2009abody 6 років тому

    Thank you

  • @senaityemanne422
    @senaityemanne422 6 років тому +1

    No gloves?

  • @Oh-ou4lp
    @Oh-ou4lp 6 років тому

    ok where is the rest

  • @asmajahan6079
    @asmajahan6079 6 років тому

    Thax dea

  • @Hu_Ns1
    @Hu_Ns1 Рік тому

    thik smear
    With out gloves
    🤔

  • @brunoasavaz8773
    @brunoasavaz8773 2 роки тому

    「コンテンツを調整する必要があります」、

  • @phali6253
    @phali6253 3 роки тому

    سلام الله على الله اذا افتهمت شي 😂🤦

  • @chandannishad4167
    @chandannishad4167 3 роки тому +1

    Samaj Nahi aarha hai .
    English girl'.
    Jara ap Hindi me batiya . Ok
    🍻😝😝😝