Blood Smear Preparation and Staining Practical Lab
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- Опубліковано 11 лют 2020
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Total WBC Count: • Total WBC count Practi...
Total RBC Count: • Total RBC Count Practi...
DLC: • Differential Leucocyte...
Haemocytometer Use: • Haemocytometer Practic...
Haemometer Hb estimation: • Haemoglobin Estimation...
Blood Smear Prep and Staining: • Blood Smear Preparatio...
RBCs in iso, hypo and hypertonic solns: • RBCs in Hypotonic, Iso...
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Your video is incredibly helpful 🙏 My little girl is in remission (thankfully) from blood cancer and I'm going to do everything I can to make sure it stays that way. Her doctor's wouldn't listen to me about her elevated WBC and she suffered for months needlessly. There is so much great material for research. Thank you for the explanations 💖
I hope your daughter gets well soon
I diagnosed my daughter at age 17 with Hodgkin’s lymphoma after she was being misdiagnosed by her GP for almost a year claiming she was in pain from a ski accident . I had no idea of any of this until one night at 3 am she came into my bedroom crying in pain. I asked for all her symptoms . I got my medical books out, and by 6 am I narrowed it down to 2 possible diseases . I ordered all her tests and biopsy. I’m truly blessed and happy to say she’s now 30 yrs young , and has given my a beautiful 1 year old granddaughter. I’m sure your little girl will be just fine . I’ll keep her in my thoughts and prayers. God bless you and your family . Stay positive. 🙏🏼❤️
Thank you. This is very well put together with all the information needed. You are highly skilled.
Excellent presentation!!! Very useful for my students. Thank you!!
Great video and very professional, many thanks.
Thank you so much...it's really helpful...I understand it more here then in college.
I have a very special purpose and vitally urgent mission, for the benefit of many others, to be able to proficiently and accurately analyze normal, abnormal and/or infected blood. Your presentation is most professional and descriptive. I am going to go over this video, over and over again, over the next two weeks, including all of the terminology, for my training.
I very recently procured a Research Lab quality trilocular microscope with 14 megapixel digital camera. I am just getting started in my training. I am greatly looking forward to your other videos, you listed, regarding Haematology.
The "Key Moments" images in your description, will be very helpful, and a wonderful reference tool for me, as well.
I do not have much time, to say the least, to become proficient. And, I have a multitude of people I need to help. Any advice you could furnish in this endeavor, that you would be so kind as to facilitate my self-education, edification and training, would be immensely appreciated. I want all of the help and training I can procure. Thank you. And, Bless you!
p.s.: I want to make sure I have requisitioned all of the equipment and tools I need to be successful in this endeavor. Would you please be so kind as to furnish a list thereof. I have already put in for everything you have mentioned in this video. Whatever I should have I want to requisition ASAP. Thank you, again!!!
your vedio is very helpfull to me every day.i can understand easyly.your explaining is great.it can understand very quckly than read note.thank you very much.
Very informative
It cleared my doubts !!!!
Extremely helpful , thank you sir 🙏
Thank u sir .
It was a very nice and informative video
Very nice and instructive video! Thanks
Nice video... Really Very helpful. Thank you🙏🙏
Thanks for explaining, this is great! Is it just me or does the blood shown at the very beginning of video look like Thalassemia minor? I see a high percentage of tear drop shaped or other misshapen RBCs, and they all seem very light colored at the edges.
You saved my practicals
Brilliant work🙂🙏
Very good explanation 👍.easy to understand. useful 👌
Nicely explained.
Thank you so much. The explanation is understandable and the steps is clear. It helps me a lot
Thanks for this video 🙏
Useful video
Thanks for sharing
Nice video, infos are greatly conveyed, thnks :D
Great work 👍👍
Uh are doing such a great job
Thanks
It helped me a lot
Thank u very good content 🙏🙏
Thank you so much !
Very clear slide
Excellent. Kindly upload Test for Blood grouping (Haemagglutination)
@ThomasTKtungnung Assuming that I properly prepared a blood slide, is there an expiration date as to when I use it for microscopy analysis?
Thank you so much🙏
V good i understand sir
Great and Thank u
Nice video ❤
Thank you sir 😇🙏🏻
Nice video.
Thank you so much sir
Nice video
Did you use a proper glass slip so the blood doesn’t touch the microscope?
Nice 👍
Thank you a lot for your effort,
Learned a lot in this video.
But I have a question,
Why don't we put a cover slide on the specimen before putting it under the microscope ?
Hope you'd answer me soon !
Rapid staining drying and observing is a routine protocol in any clinical lab. But you can also add coverslip for long lasting slides. Offcourse it will add extra time to the slide making process. Add a few drops of mountant (DPX, Canada balsam, Euparal etc) ,apply a rectangular coverslip and then wait atleast 24hours for mountant to dry, before using the slide.
@@TheSingtangpaScienceGuy Is there a time period in which to apply stain, or we can wait for some time before staining the slide? What happens if we stain after few hours vs after a few days?
if a blood smear is left overnight to excessively air dry, will that lead to a different staining? Because I (accidentally) did just that during MGG-staining, the one stained the day after had absolutely NO purple staining of the nuclei, they were only a light blue.. I figured it was due to a lack of hydrophobic stabilization i.e. romanowsky effect.. then again the buffer solution has water but maybe it just didn't have time to displace the ethanol and rehydrate the blood smear in the few minutes it was being stained
It's is amazing 🤩
Tq sir, super sir
Hello,sir
Try to make a video on DLC differential leucocyte count. Great work 👍🤗
Here's the link to my DLC video ua-cam.com/video/VFKm_kMTf50/v-deo.html
What is the function of white blood cells basophilic and acidosis?
Thank you .But what is the 4 membrane structure in platelets
Thanks for pointing out a small mistake in wording. I meant 4 zones: Peripheral, Sol-gel, Organellar and Membranous zones. So its a 4-zoned structure.
Thank u so much
Tnk u so much🙏
Rất cảm ơn những vid có ý nghĩa như,mong nhiều càng vid như này chứ không phải vid nhảm như bọn nhảy như mấy chú vịt:))))
I'll believe Google translate and take this as a complement .
Nice
Thanks
Sir what is the reactive lymphocytes plz reply
how will you ensure an adequately prepared thin blood film in a polycythaemic sample
I have no special training in Medical Laboratory Technology so i cant help u much with the detailed aspects of clinical lab procedures. But from experience, i can suggest a few tips. If you want a thin smear, use a very tiny droplet of blood sample and position the smearing slide at an angle less than 45 degrees to the slide containing the blood drop. Alternatively, you can thin the polycythemic blood by diluting with an appropriate solution such as isotonic saline or perhaps an anticoagulant such as EDTA. Hope that helped.
Assuming that I properly prepared a blood slide, is there an expiration date as to when I use it for microscopy analysis?
the colors will fade over time, but I'd guess it could last for years or even forever, and you'd still be able to distinguish the granula etc.
Could you please tell me that why we disinfect with alcohol our finger before pricking?
The answer is already in your question 🙂 To Disinfect.
In order to minimize/nullify the chances of germs entering your body through the pricked site.
Which course was u studied ?
What is Leishman's stain
Super ❤️💖👍👍✨😄👍
Hello, Sir! Do you think this is the right procedure of blood smear staining? Whole Blood> Centrifugation> Blood Serum> Fixative> Stained Cellular Debris> Primary Staining> Rinsing> Stained Target Cell> Morphological Identification
I hope you will answer sir , Thankyou!!
I'm not very aware of this procedure. Serum doesn't contain the blood cells. If you process the centrifugal residue, that's a different thing.
@@TheSingtangpaScienceGuy oh, thankyou sir, but may I know what is stained cellular debris and stained target cell? Is this under the morphological identification?
This is actually my assignment, It’s a flowchart but I couldn’t find the exact procedure on the internet. this is the only video that helped me to have an idea abt this, thankyou sir
@@slm.5496 I'm not sure what your teachers are expecting you to find in serum. If your assignment is to stain and identify blood cells, then the above flowchart you mentioned is faulty. You can basically follow this video for that. But if they are expecting you to find some cellular entity in serum, then you follow the flowchart. Your flowchart is totally new to me. Are you pursuing some higher level clinical/immunological biology course?
selamat nge laprakkkk
For smear how much are drop on a slid
Hi there. If I’m just using a microscope at home… is there anything round the home I can use as stain? Does it need to be fixed? If so, why? Also.. want to try and look at a live sample?
try searching for Methylene blue at your local drugstore and cosmetic store (banned from public access in some countries though). Not the most ideal stain for blood but u might have some luck with it as its a nuclear stain. Yes blood smear slide needs to be fixed, else the cells will all look mushy under the microscope (simply put). For live samples, just take a drop of blood on a glass slide, cover with coverglass and view under mic. you may optionally mix it with a few drops of normal saline (isotonic saline) before viewing.
@@TheSingtangpaScienceGuy ok, will do. Thank you! Working on ordering some stains! If I’m trying to look for/ find parasites & bacteria in blood, as well as different tissue samples; would you be willing to kindly explain to me the best simple steps possibly to do that? Or is it the same? Thank you so much!! 🙏🏻👍🏻
Kindly upload Blood Cross matching
Thanks thanks
Hello, thank you for the video
May I share it with my pupils on my UA-cam channel in Arabic language ?????
no picture of hypolobated neutrophil?
Please mention in written form also its really helpful forme❤
𝚃𝚑𝚎 𝚟𝚒𝚍𝚎𝚘 𝚍𝚎𝚜𝚌𝚛𝚒𝚙𝚝𝚒𝚘𝚗 𝚊𝚕𝚕𝚘𝚠𝚜 𝚘𝚗𝚕𝚢 𝚊 𝚕𝚒𝚖𝚒𝚝𝚎𝚍 𝚠𝚘𝚛𝚍 𝚌𝚘𝚞𝚗𝚝. 𝚂𝚘 𝚝𝚑𝚎 𝚎𝚗𝚝𝚒𝚛𝚎 𝚜𝚌𝚛𝚒𝚙𝚝 𝚌𝚊𝚗𝚝 𝚏𝚒𝚝 𝚝𝚑𝚎𝚛𝚎. 𝙸 𝚊𝚖 𝚙𝚕𝚊𝚗𝚗𝚒𝚗𝚐 𝚘𝚗 𝚖𝚊𝚔𝚒𝚗𝚐 𝚊 𝚠𝚎𝚋𝚜𝚒𝚝𝚎/𝚋𝚕𝚘𝚐, 𝚠𝚑𝚎𝚛𝚎 𝚊𝚕𝚕 𝚖𝚢 𝚟𝚒𝚍𝚎𝚘𝚜 𝚒𝚗 𝚠𝚛𝚒𝚝𝚝𝚎𝚗 𝚏𝚘𝚛𝚖 𝚠𝚒𝚕𝚕 𝚋𝚎 𝚞𝚙𝚕𝚘𝚊𝚍𝚎𝚍. 𝙳𝚘 𝚜𝚝𝚊𝚢 𝚝𝚞𝚗𝚎𝚍.
Why doesn’t the distilled water rinse cause the blood cells to lyse?
What is the name of this method
What happens when you add equal drop of distilled water to leishman stain?
Dilutes the stain to 50% of its initial concentration. This is supposed to result in a well balanced staining of the corpuscles. I dont know the exact mechanism though
Is oil drop needed in every magnification or is it just for 100x ?
Only oil immersion lens requires a oil interface between specimen and glass. Otherwise you wont visualize anything. The immersion oil is a specially thick oil with refractive index quite close to that of glass.
@@TheSingtangpaScienceGuy ok sir
And this lens is used in most cases, right ?
So we need to have a drop of oil in every magnification while using this.
@@DocAgarwal 100x is used mostly only in Blood cell and bacterial studies and other cells and structures not resolved under 40x. Its use is limited. But very useful. Use oil only for 100x
@@TheSingtangpaScienceGuy ok sir
Thank you so much !!
good job
Best
Hello, I belong to an engineering background but working on the CBC analysis. Can you pl tell what you do after you get the total number of rbc, wbc and platelets. For instance i counted 700 something rbc, by what formula does this count changes from few hundreds to millions of cells per microliter? If you do know pl share for rbc, wbc as well as platelets. Thanks in advance
Please watch my videos on rbc, wbc and platelet counts and also my video on the working principle of haemocytometer. Just go to my channel page, then click Playlist and click the HematologyLab . Your query will be answered in my videos.
@@TheSingtangpaScienceGuy is it possible to give all of their counts by checking on the DLC glass slide? not the hemocytometer? Supposedly if we can count every rbc in the observation region?
Not just here but tho great intro info, find one showing the model of hematology microscope Used? Ugh! Mystery! Best near one a CX31 by olympus?? Any reply greatly Appreciated! Former lab tech many years ago, TX!
Hi warren, sorry to say this but i simply couldnt make out wat ur trying to say 🤷🏽♂️ i'm sure ur phone's autocorrect/autofill feature is at play here !
Thank you sir
Beautiful explanation ☺️..clear voice 😿
Had a cold few days prior to voice recording so... 🤷🏽♂️😁
Can we see RBCs without any staining under microscope?
Yes but the morphology can't be decided
😊😊😊
Hemorrhagic smears ,ano po yon?
Can't we see this from normal and small microscope that is available in any place is this is important to take this type of microscope
You can use any compound microscope with atleast 40x objective lens to view blood cells
Can you make a blood smear of vaccinated person vs unvaccinated person.
Or person who was unvaccinated vs same person after vaccination.
This test conducted by you followed by honest result will really help to identify if the vaccine is safe or not.
If there is any change in shape of blood or anything else
Your requested video is Ready. ua-cam.com/video/JOnevchDMPI/v-deo.html
111 Comments 🙏🙏💛💛
Jai Shri Gajanand Ganpati Bhagwan ki Jay 🙏🙏💛💛
Methanol is not required?
Hi. Thats a good question. In leishman staining protocol, separate Methanol fixation is not required because Leishman stain is nothing but methanol itself (into which leishman stain powder is dissolved). However in other blood staining protocols where the stain is anything but methanol based , then u need prior methanol fixation of the blood film. Hope that answered ur query.
@@TheSingtangpaScienceGuy thank you so much.
ام المختبر دزت هذا فيديو نسوي بالمختبر وهذا يرطن واني كلشي ما فاهمه 😂😂
Hi, can I ask if you also used the blood serum?
Whole blood, no centrifugation, as can be seen in the video.
@@TheSingtangpaScienceGuy can i ask if when do you use the centrifugation? and also, the morphological identification?
this is actually for my assignment in gen bio☺️ thankyou for answering!!
@@rayhanagamor9039 centrifugation to get plasma is done mainly in some blood tests. For some other tests, blood is allowed to coagulate to obtain serum. And for most microscopic blood work, a drop of blood from pricked finger is used. For morphological identification, you have to practice a lot, but its not that difficult, you can just follow my video and practice identifying. Download random WBC photos from internet and assess your identification skills. Good luck for your assignment!
@@TheSingtangpaScienceGuy can I ask another questions?
Is the blood serum also use in other blood test? and also in the process of blood smear, what comes first? rinsing before fixative and then staining or is it fixative then staining and then rinsing? I hope you’ll answer these questions, thankyouu!!
111 Comments 🙏🙏💛💛
Thanks Alot Universe 🙏🙏💛💛
One question. Where are the 40X images?
Hi. I didnt incorporate 10x and 45x images in the video because blood cells are still a bit too tiny under such magnifications. Only 100x oil immersion images are included in the video
@@TheSingtangpaScienceGuy Do you have the pictures? Can you share them with me? Plis.
@@ivannapabonabshana8279 will have to go thru my image files. Please tell me which specific images you want and under which magnification(s)
@@TheSingtangpaScienceGuy Thank You.
I need monocyte, Basophil, neutrophil, eosinophil, monocyte and lymphocyte in 40X.
@@ivannapabonabshana8279 all of these cells are there towards the end of the video. You can take a screenshot of these cells and use.
What is your microscope magnification x ?
1000x
@@TheSingtangpaScienceGuy thank you
We remove the first drop .No??🙄
Hi thats a good question. The common practice of "Discarding first drop" is applicable when we want to do a specific quantitative test on the contents of blood Example: Blood glucose, blood cell count etc. The practice removes faulty results that can arise from unnecessary dilution of the blood drop with the surrounding tissue fluid. The aim of this video experiment is not to quantify anything but to just look at the blood cells under the microscope after staining.
If i was making a video where i want to, say, do a RBC or WBC count, or estimate the amount of something contained in the blood, then i have to discard the first drop by all means. If not, that should raise eyebrows on how i performed the experiment. Hope that helps 🙂
@@TheSingtangpaScienceGuy Yaa, it is really helpful .Thank u so much ..keep it up!❤️😊
Yes
Wrong....start with 10x lens then shift it to 40 then 100...doing what you said might break your slide...
How? Will it create pressure on slide to break?
ഇവിടെ മലയാളികൾ ഉണ്ടോ😎
Undallo
You don't need all of those. Livo A700 completely automated the entire process of Morphology. just watch the following Livo A700 video.
Can you make a blood smear of vaccinated person vs unvaccinated person.
Or person who was unvaccinated vs same person after vaccination.
This test conducted by you followed by honest result will really help to identify if the vaccine is safe or not.
If there is any change in shape of blood or anything else
hi anuj, your requested video will be uploaded within the next 1 week. I'm currently working on it. Will show you not only blood before and after vaccnation, but will also show you blood before and after covid infection.
@@TheSingtangpaScienceGuy Thanks Thomas !!!