I'm having trouble designing a guide RNA (gRNA) for a gene that's small, around 2300 bp, with 3 exons and 2 introns. The first two exons overlap in the 5' UTR region, and the third exon is partially in the 3' UTR. While using CHOPCHOP and CRISPR-P software for gRNA design, I'm not getting good results. The top-ranked gRNA has off-targets in exons of other loci on different chromosomes. My question is, how safe are off-targets, and if the mismatches or off-targets are in intergenic regions, should we consider using that gRNA? I'm quite confused about this. Please if it is possible for you kindly help
These are generally less concerning since these regions don't typically code for proteins or play direct roles in gene regulation. However, off-targets near regulatory elements or non-coding RNAs could still pose a risk
@@asifmolbio after doing crispr when we grow plants and then send T0 generation for cas9 sequencing to company for seeing which samples has gene knock out. That data
Is there any method or software to simulate link of epigenetic changes to disease. We get data of gene to disease link. I want to know the epigenetic link to disease. Please guide me.
Much appreciated your efforts. Kindly make a complete series of lectures on Bioinformatics for CRISPR genome editing.
Thanks Obaid Ullah, yes i am planning to record lectures on this
Thank you provide such a nice and helpful video's
Glad it its helping
@@asifmolbio Dear Brother whenever you visit the Beijing, Remember me
@@DilawarAbbasPhD thanks so much sure please add me asifalikalas its my we chat ID
@@asifmolbio i try but unable to find can you please add me Dilawar512110
I'm having trouble designing a guide RNA (gRNA) for a gene that's small, around 2300 bp, with 3 exons and 2 introns. The first two exons overlap in the 5' UTR region, and the third exon is partially in the 3' UTR. While using CHOPCHOP and CRISPR-P software for gRNA design, I'm not getting good results. The top-ranked gRNA has off-targets in exons of other loci on different chromosomes.
My question is, how safe are off-targets, and if the mismatches or off-targets are in intergenic regions, should we consider using that gRNA? I'm quite confused about this. Please if it is possible for you kindly help
These are generally less concerning since these regions don't typically code for proteins or play direct roles in gene regulation. However, off-targets near regulatory elements or non-coding RNAs could still pose a risk
please details one video cloning / preparation of gene construct for crisp cas 9
Sure thanks for recommending a potential topic, I have added it into upcoming list
❤
How to interpret data of crispr
Which data?
@@asifmolbio after doing crispr when we grow plants and then send T0 generation for cas9 sequencing to company for seeing which samples has gene knock out. That data
Can i discuss with you? Any contact ?
Is there any method or software to simulate link of epigenetic changes to disease. We get data of gene to disease link. I want to know the epigenetic link to disease. Please guide me.
Sorry i don’t know any for this