Could you please explain this to me sir - if we get a skin cell at a crime scene and then we extract the dna from it, that's 6.6 billion base pairs right? Do we restrict the entire DNA (6.6 billion base pairs) with restriction enzymes and do an rflp analysis?
When this was done regularly, all the DNA was digested and then just small segments were visualized by using radioactive probes of gels. This let people look at individual RFLPs because the probes bind specifically to the sites and not to other DNA. These days, forensics are done with polymerase chain reaction of just a few repeat sites. Polymorphic regions are amplified and then analyzed for differences in length because of differences in the number of repeats at these sites and this is used for forensic identification, and paternity testing. This is what the FBI uses: www.fbi.gov/services/laboratory/biometric-analysis/codis For something about repetitive DNA, try here: dnaftb.org/31/animation.html
Thank you for a very thorough and professional presentation of this process.
Thank you for doing this. You explain things slowly and helpfully. I know understand everything for my exam. Thank you!
Inspirational, from any perspectives! Thanks for the upload.
I now have a new knowledge on another protocol for DNA extraction. Thanks so much.
Thank you for uploading this! Learnt a lot🙌 very well explained ❤️
Why so "physically" !!!!!
What about isolating RNA? I'll take any decent protocol even if I have to still use bought reverse transcript, and and say reusable magnetic beads.
very simple way to explain it thanks :)
Do we have to do ALSO do phenol-chloroform extraction after we use the lysis resin technique in order to isolate/extract DNA?
Great, thank you so much.
How to get the protocol
Outstanding! Thank you!
Could you please explain this to me sir - if we get a skin cell at a crime scene and then we extract the dna from it, that's 6.6 billion base pairs right? Do we restrict the entire DNA (6.6 billion base pairs) with restriction enzymes and do an rflp analysis?
When this was done regularly, all the DNA was digested and then just small segments were visualized by using radioactive probes of gels. This let people look at individual RFLPs because the probes bind specifically to the sites and not to other DNA.
These days, forensics are done with polymerase chain reaction of just a few repeat sites. Polymorphic regions are amplified and then analyzed for differences in length because of differences in the number of repeats at these sites and this is used for forensic identification, and paternity testing. This is what the FBI uses: www.fbi.gov/services/laboratory/biometric-analysis/codis
For something about repetitive DNA, try here: dnaftb.org/31/animation.html
Could you give me the reference of the protocol that you use please?
DNA Learning Center Barcoding 101 website, dnabarcoding101.org/
is dna barcoding a dna isolation ? i mean another name for dna isoltion ?
DNA barcoding is using DNA sequence from small regions to identify species.
Check here to learn more: dnabarcoding101.org/
Hey
Any biologist here can help me?
Please don't open reaction tubes with one hand ... crosscontamination risk