CELL FRACTIONATION | Homogenization | Centrifugation | Techniques Used in Cell Biology

This lecture is about cell fractionation. A technique used in cell biology. cell fractionation consists of two steps,
Homogenization
and centrifugation.
i have explained homogenization and different conditions that are necessary for homogenization. i have also explained centrifugation and its types, Hope you will enjoy the video. Subscribe our channel for more Updates. Chapter 1, cell structure and function: Topic 1.1 Techniques Used In Cell Biology . sub Topic 1.1.1 Cell Fractionation #centrifugation #homogenization #cellfractionation #prefirstyear #onlineclasses

[@SufiyanAbdulrasheed]

Sir apna baht acha samjeya

[@BiologyTerms]

❣️❣️

[@zazaikhan979]

Sir u are the real hero, sir please do it for the sake of Allah we are very gladful and thankful to u. Keep it up

[@BiologyTerms]

Thanks alot dear❤️❤️

[@Selflovexpedition]

Thank you 😊 sooo much ❤️ sir for clearing all my doubts

[@BiologyTerms]

Welcome

[@shaawanashahjehan5797]

I just love the lecture .................. Outstanding !!!!!

[@jaxtonjase7189]

Instablaster.

[@eshaalkhan1612]

your teach is very well.

[@BiologyTerms]

Thanks alot

[@eshaalkhan1612]

allah apko kush rakhy

[@BiologyTerms]

Ameen

[@zawedaslam1518]

Your way of teaching is good......and plz upload more videos 😊

[@BiologyTerms]

Thanks

[@azraasma3765]

Thank u so much
Sir
Allah subhanallah aap ko behtareen agar aatah farmaiye Aameen

[@BiologyTerms]

آمین!

[@ankitadahiya3797]

@@BiologyTerms ye Kya likha h I can't understand this language

[@BiologyTerms]

kidr kya likha hy?

[@ankitadahiya3797]

@@BiologyTerms Urdu se

[@BiologyTerms]

Ameen likha hy

[@anushkavatsa6750]

Yaar pahle kyun ni dekha
Bht accha explain kiya hai aapne bhai

[@BiologyTerms]

Thanks

[@AbdulSattar-me8en]

Sir I like your teaching

[@BiologyTerms]

Glad to hear that

[@sunnyproduction7946]

Thanks for clearing my doubts

[@BiologyTerms]

It's my pleasure

[@fakhraamjad9309]

You teach very well.
Keep the good work up👍

[@BiologyTerms]

Thanks

[@BiologyTerms]

keep watching

[@sardarzaeemsandhusardar3817]

Absolutely stunning work sir really appreciate your kind efforts,may allah bless you always for his mighty name sake...am zaeem from Islamabad city lecturer at English literature,it's really honour to meet you.keep up the gorgeous work

[@BiologyTerms]

💕

[@afaqahmad8368]

Thank you so much to clear my concept

[@humabatool2873]

Nice lecture

[@BiologyTerms]

Thanks

[@AishaKhan-gg2ee]

Sir baki lectures please

[@khankakar3163]

Bro plzz regular lecture upload kero,you lectures are too good

[@BiologyTerms]

InshaAllah i will upload regularly ❤️

[@misbahmemon3182]

Outstanding sir

[@BiologyTerms]

❤️

[@everythingtechpro4]

i was searching for someone to teach biology lessons and your lectures are great .Keep it up
just dont repeat the words again and again you sometimes do it
keeep it uppp

[@BiologyTerms]

I will try my best

[@ZaidKhan-zw7oq]

Keep it up sir ☺️

[@summaiyasami8573]

Nice word sir

[@tayyabayousaf4243]

Nice

[@shahidhassan253]

Plz upload more vidoes as soon as possible 🙏🙏🙏

[@BiologyTerms]

InshaAllah i will complete first chapter videos in this week

[@shahidhassan253]

@@BiologyTerms ❤️ We love the way U teach !! Allah bless u

[@BiologyTerms]

Thanks alot for appreciation ♥️♥️♥️♥️ stay blessed

[@aena_]

Very good video

[@sakshibansal4433]

Thank u sir 🙏

[@aena_]

Thanks

[@faraseenkhan2853]

Asalam walikum sir homogenate formation me water include hoga ya nahi means Us grinder me jab hum tissue dalenge to us me water bhe dalenge grinder machine me ke nahi ??

[@BiologyTerms]

During homogenate formation for cell centrifugation, water is typically added to the tissue. This is done to create a suitable buffer or medium for homogenizing the tissue and breaking down cell membranes. The addition of water helps in the disruption of cell structures and allows for the isolation of cellular components during subsequent centrifugation steps. The specific buffer or solution used can vary depending on the experiment's requirements, but water or a water-based buffer is commonly employed.

[@misbahmemon3182]

Sir aap federal parate ho ya Punjab ya sindh

[@CaptNia]

Hello sir over all merey sarey concepts clear hain but i have some confusion at certain points.sir mera ak question hea k aap ney kaha k temperature hum low rakhtey hain takey enzyme inactive ho jayn tu sir sath main aap ney agey yea bhi kaha hea is mixture main hum kuch enzymes bhi add kertey hain tu sir woh kesey kam kertey hain itney low temperature per kindly is point ko clear ker dain

[@BiologyTerms]

Homogenization at low temperatures is indeed aimed at preserving cellular components and minimizing enzymatic degradation. However, certain enzymes like cellulases and pectinases can be used during homogenization for specific purposes, even at low temperatures.
Cellulases and pectinases are enzymes commonly used in plant cell fractionation to break down the cell wall components, namely cellulose and pectin, respectively. These enzymes help to disrupt the plant cell walls and release the cellular contents for further analysis or isolation of specific organelles.
When using cellulases and pectinases during homogenization, it is common to perform the enzyme treatment at a temperature that optimizes their activity, which is typically around room temperature (20-25°C). The enzymes are added to the cell suspension, and the mixture is incubated for a specific period to allow the enzymes to act on the cell wall components. The incubation time will depend on the specific enzyme used and the plant material being processed.
After the enzymatic treatment, the sample is typically further processed through centrifugation or filtration to separate the disrupted plant cells and cell debris from the released cellular components. This allows for the isolation of organelles or other cellular fractions of interest.
It's important to note that the use of cellulases and pectinases during homogenization is specific to plant cell fractionation or when studying cell wall components. In other cell types, where cell walls are not present, the use of these enzymes may not be relevant or necessary during the homogenization step.

[@beinghuman4457]

Sir what about protease inhibitors ?

[@yusraakbar8362]

I understand your method only

[@misbahmemon3182]

Sir aap abhi Jo new book aya hai us main se parae Sindh ka please

[@Sir_Ramzan]

Kis board ka h sr

[@BiologyTerms]

Federal board

[@SufiyanAbdulrasheed]

Sir bath acha samjaye

[@BiologyTerms]

❣️❣️

[@infoofscience8090]

Sir bio ki book complete kara de

[@BiologyTerms]

Ok beta Possible hwa to kar dain gy

[@misbahmemon3182]

Sir aap na Sindh main parae is chapter main se shuru karo

[@khanopkhanop7057]

Hi

[@misbahmemon3182]

Sir please

[@misbahmemon3182]

Sir aap please Sindh parae na

[@misbahmemon3182]

Sindh parao

[@BiologyTerms]

both federal and punjab parhta.. Sindh board ki new book pdf main hy? ap k pass?

[@misbahmemon3182]

Sir kaha par hai pdf main please sir parao

[@BiologyTerms]

okk

[@hamnamanzoor6677]

Ak hi baat 600 bar ni bolein to r b achy sy samaj ata...ak hi baat 600 bar bol k lectire lamba kar liya

[@BiologyTerms]

G ok

[@irfansabir1409]

Thanks