Cell fractionation | Basic technique of Cell biology | Video 19

A useful technique for studying cell structure and function is cell fractionation which takes cells apart and separates major organelles and other subcellular structures from one another.
The piece of equipment that is used for this task is the centrifuge, which spins test tubes holding mixtures of disrupted cells at a series of increasing speeds. At each speed, the resulting force causes a subset of the cell components to settle to the bottom of the tube, forming a pellet.
At lower speeds, the pellet consists of larger components, and higher speeds result in a pellet with smaller components. Cell fractionation enables researchers to prepare specific cell components in bulk and identify their functions, a task not usually possible with intact cells.
For example, on one of the cell fractions, biochemical tests showed the presence of enzymes involved in cellular respiration, while electron microscopy revealed large numbers of the organelles called mitochondria. Together, these data helped biologists determine that mitochondria are the sites of cellular respiration. Biochemistry and cytology thus complement each other in correlating cell function with structure.

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Sir AP ka writing bohot chota ha Nazar nahe Aata😢

[@malikawan8123]

Thank you so much sir for this great lecture 💖💖💖💖

[@muhammad_younas]

Sorry sir... centripetal force ni centrifugal force 😮😂

[@AbdUllah-i2v]

Thanks sir❤

[@hackerpakistan8985]

you made few linguistic and technical mistakes in this video. hope you will consider this comment positively and improve yourself.

[@Esha_animation]

Can you specify his mistakes

[@Miansadiqahmad]

Mistakes?

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sir
colorimeter and Spectrophotometer
per EK video

[@BAZMEKHOWAR190]

Very pooor lecture. This person is himself confused about centrifugal force. So don't waste your time.

[@rohitprajapati1374]

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[@nasirabbasi295]

The force which compl the body to move in circle is called centripetal force

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Good lec

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Ram ram ❤

[@aakanshayadav9613]

This topic was quite confusing for me but the way you have explained it's amazing and it's clear now ,

[@taimoorkhan8165]

Great sir

[@nehmatfaraz4417]

Nice

[@fazalsubhan4079]

Thank u

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Very helpful sir 👍

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Very heplful
Jazakallah

[@saqib...]

If you want to get ribosome than you have to spin the centrifuge for 3 hours at 150000g.

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its amazing but bro microsome is the fragment of endoplasmic reticulum

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microsome broken pieces of membrane ko boltay hain are you sure ??????????? 🙄

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Thanks dear sir

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Very well explained

[@AbdulHameed-cb5ec]

Masallah

[@thebiologyspecialist4444]

For detailed lecture on cell fractionation
ua-cam.com/video/_ILwdrDT0TM/v-deo.html

[@arsalnali4425]

Sir aap ne bhola k DNA ko separate karne k liye pehle aap ne nuclei k cells membrane ko break karna hoga Tu is k liye kitni gravity our kitna time required hoga

[@imdadali1436]

Sir lecture acha hy pr white board pr likha saii nazr NY a raha

[@MuzamilKhan-j6q]

Outstanding sir thank you 👍

[@TheCreativeVlogger]

2:42 not centripetal force it's centrifugal force...

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Great Sir

[@hackerpakistan8985]

at 2:44 you said centrifugation machine uses centripetal force ? why not centrifugal force. waiting for answer

[@rafiaiftikhar5888]

Broh, centrifugal and centripetal force are same in magnitude as they both generally make the components move in a circular motion. Lol, 😅

[@hackerpakistan8985]

@@rafiaiftikhar5888 if both forces are same in magnitude then how come the centrifugation occur. shouldn't they cancel out????

[@zakirakhanam4528]

Thank you sir ...this is really very helpful ❤

[@SahibzadaUmair-i8u]

Best lectures 😊

[@miss_pavu_official]

Thank you sir 👍🏻

[@RajaBilal-is7iw]

It was realy a great lecture

[@zulqarnainaslam-og2ci]

Well-explained❤