When you want to release volume into a vial.. you mentioned how you must first go to the first stop than hold ? When you say hold do you mean still have your finger held in place in the first stop ? Then you again until you hit the 2nd stop ?
Yes: Stop at the first stop. That delivers the full volume you set on the pipette. You will see a tiny amount of residual liquid left inside the pipette tip. To empty the tip completely, move the pipette over a different container then press the plunger all the way down.
You could cite this video the following way: Rainin, Laboratory Solutions from METTLER TOLEDO, 24 October 2014, Good Pipetting Techniques from Rainin, [video], UA-cam.com, ua-cam.com/video/X73R_4cntpA/v-deo.html
Dial above the pipette's maximum volume, then dial down from there. For example, on a 200 µL pipette, dial to ~205 µL then dial down to set. The pipette will allow you to do this.
No. Pos-D has its own tips, sometimes called "capillaries" and sometimes called "syringe tips." Each Pos-D tip has its own built-in plastic piston. The little metal "jaws" inside a Pos-D grab the top of that inner plastic piston to move it up and down. It's a different mechanism from regular air-displacement pipettes that use universal tips. Pos-D is positive displacement: no air between the sample and the end of the piston inside the Pos-D tip.
we love 30 minute videos about a pipette!
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most comprehensive video on pipetting I've ever come across. thanks!
Glad it was helpful!
Very educational. Thank you.
Excellent learnt many things though using pipette for 10yrs
Thanks for watching!
Thank you for the good techniques!
Thank you for watching it
Very informative and helpful thank you!
Glad it was helpful!
Thank you for this video!
Glad it was helpful!
Sorry how does having a lower concentration of 0.74 end up killing all the mice? wouldn't you be using less
When you want to release volume into a vial.. you mentioned how you must first go to the first stop than hold ? When you say hold do you mean still have your finger held in place in the first stop ? Then you again until you hit the 2nd stop ?
Yes: Stop at the first stop. That delivers the full volume you set on the pipette. You will see a tiny amount of residual liquid left inside the pipette tip. To empty the tip completely, move the pipette over a different container then press the plunger all the way down.
@@METTLERTOLEDOLaboratory wow.. now I understand why my calibration curves always have a low R^2 😅
how would the apa citation of this video be?
you shouldn't really cite videos in scientific work, annoying I know but still x
You could cite this video the following way: Rainin, Laboratory Solutions from METTLER TOLEDO, 24 October 2014, Good Pipetting Techniques from Rainin, [video], UA-cam.com, ua-cam.com/video/X73R_4cntpA/v-deo.html
Lovely!
How can we use dial down technique for 100% desired volume
Dial above the pipette's maximum volume, then dial down from there. For example, on a 200 µL pipette, dial to ~205 µL then dial down to set. The pipette will allow you to do this.
Can I use universal pipette tips on a pos d pipette?
No. Pos-D has its own tips, sometimes called "capillaries" and sometimes called "syringe tips." Each Pos-D tip has its own built-in plastic piston. The little metal "jaws" inside a Pos-D grab the top of that inner plastic piston to move it up and down. It's a different mechanism from regular air-displacement pipettes that use universal tips. Pos-D is positive displacement: no air between the sample and the end of the piston inside the Pos-D tip.
Thank you- very informative
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Thank you very much
You are welcome
Thank you very good
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Good very good
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Fantastic
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most comprehensive video on pipetting I've ever come across. thanks!
Wow, thanks!