Many have a question with regard to why it is 10^4 (@5:42) for concentration of cells per mL: Now recall/remember Watch video @1:42 to what they say with regard to volume at each square. It occupies a volume of 10^-4 mL i.e 0.0001 mL = 0.1 µL. Therefore for calculating - concentration of cells "per mL" make this 0.1 µL to 1 mL (multiply with 10^4 to get 1 mL). Hope it helps
i wish every single labs out there had beautifully edited constructed with great explanations like this... would definitely make uni life way more interesting... amazing video, hats off!!!
I've watched so many videos on how to count the cells and you are the best ever who explained it properly, I can understand you very well with your speech and you explained it properly, thank you,
Very good video, but one mistake. At the end, she says that 94.7% of the 216,000 cells/mL are viable. However, she uses the number of viable cells (54) to calculate average number of cells/square. If she had used the number of total cells (nonviable + viable, or 57) to calculate cells/square, then in the end it would make sense to take the final number and say that 94.7% of those cells are viable.
@@mjunchai630 so before you reached that step, it was calculating the concentration of cells in a square (which as he said is 10^-4 ml). We want to express our concentration in terms of cells per ml though, so we multiply by 10^4, essentially doing unit conversion.
Really easy to understand and great use of simple graphics, I'm just looking into yeast counts for brewing. If I can follow this anyone can, thank you.
Mam can you explain if the RBC count is more in corner than centre so we only count center's RBC and not corner then report me make has cause an errors to diagnose patient 🤔🤔🤔🤔🤔🤔🤔🤔. Please tell about this
But when we do subculture how much amount of media or cell suspension will be required ..please explain that one also...and thanks for your good explanation
Is 10^4 is the depth factor?? At calculating the cell number Anthor question ,I know that if we have small cells(bacteria) we will count at the central square only and if it large cells(fungi) we will count the four squares on corners only, is that true?
hi question! if they so much as touch the bottom and right one, are they already excluded from the count? like if they touch the first line of the bottom and right lines. thank you!
We use this video in an introductory cell biology lab course, it's very informative and helpful! However, the linked portion where you can print off a certificate at the end appears to be broken. This has been a great mechanism for giving students credit for watching the video. Do you have any plans to make that part active again? Cheers!
Ahoy! Apparently there's no way to tag a channel in here nowadays and therefore no way that I'm aware of you get your attention, BioNetwork. So I'll just reply to my own comment and see what happens. Please take a moment to address the concern in my above comment. Thanks!
Really informative video! Although I understand the calculation for the percentage viability, I'm just wondering why the average number of cells per square (10.8) isn't rounded down; as it is not possible to have 0.8 of a cell? Thanks!
Ultimately, the purpose of the calculation is to determine the concentration of cells. It is true that it is not really possible to have 0.8 of a cell. However, the exact number, 10.8, is used in the calculation for the concentration of cells for a more accurate result. Hope this helps!
Amazing video indeed. can somebody please tell me about which tryphan blue solution is used i=here. can I use tryphan blue 1 % in dH2O. Need help. Thanks
thanks for the great video:) I wanna ask, is there any other alternatives for trypan blue? like common stains that easy to prepare or obtain? can methylene blue be used instead of trypan blue?
Great question! Methylene Blue is unsuitable for cell viability as it will stain both live and dead cells equally. You can try automated counters, fluorescent dyes (propidium iodide), or colorimetric assays (MTT, MTS, WST-1) instead. However, all these methods will require you to have specialized equipment to test the viability of cells. Trypan blue is a much cheaper alternative.
Can anybody please explain, I want to count cells with computer by taking an image of the simple and I need to know what chamber one uses to do this? Am I correct in my understanding that I require a counting chamber but not a hemocytometer?? When I look the term counting chamber it keeps comming up with a hemocytometer but I don't desire to manually count cells.
Is that a standard light microscope? In my readings it says that a fluorescent microscope is required to detect the dye that distinguishes living from dead cells.
Irram Sanna Because each large square houses 10^-4 mL of liquid. You multiply by 10^4 to find the number of cells that would be in a whole milliliter of liquid. This makes your units: cells/mL
I don't get that you've used average # of viable cells/square to measure the concentration and at last, you mention that 94.7% of that concentration are viable. Shouldn't you be using total # of cells per square and then use it to determine total concentration, of which you'll have 94.7% viable cells concentration?
One large square (or with depth cube) has a volume of 0.1 µL. Since you want to calculate the concentration of cells per mL (1 mL is 10000 times more than 0.1 µL) you multiply the number of cells per large square (10.8) by a factor of 10000 (10^4) to get to the number of cells per mL. You also multiply by 2 because you diluted the sample with the dye.10.8 * 10^4 * 2 = 2.16 * 10^5 cells/mLor10.8 * 10000 * 2 = 216000 cells/mL
Many have a question with regard to why it is 10^4 (@5:42) for concentration of cells per mL: Now recall/remember Watch video @1:42 to what they say with regard to volume at each square. It occupies a volume of 10^-4 mL i.e 0.0001 mL = 0.1 µL. Therefore for calculating - concentration of cells "per mL" make this 0.1 µL to 1 mL (multiply with 10^4 to get 1 mL). Hope it helps
Thanks I was struggling with that. Cheers
Thanks a lot❤
Why she said each square contains 10^-4 ml even though 200ul/9 squares gives 22 ul in each square we should have 22 ul
i wish every single labs out there had beautifully edited constructed with great explanations like this... would definitely make uni life way more interesting... amazing video, hats off!!!
This isnt beautiful. It's boring and tedious as hell.
Wouldn't it be better if you did them yourself?
@Gatlin Ronnie Pretty sure no one here cares. Promote it at instagram hacking tutorial, more people will care
I've watched so many videos on how to count the cells and you are the best ever who explained it properly, I can understand you very well with your speech and you explained it properly, thank you,
Excellent work. It's crystal clear and systematically explained. Thank you for putting this video up.
Your videos have given me great teaching aids to students who are new to cell culture! Thank you!
amaaaaaaaaaaaaaaaaaaaazing ,,,,, really amazing
excellent photographing ... direction.... sound effect... commentary ... subtitle .. perfect timing.
science is different actually
Very good video, but one mistake. At the end, she says that 94.7% of the 216,000 cells/mL are viable. However, she uses the number of viable cells (54) to calculate average number of cells/square. If she had used the number of total cells (nonviable + viable, or 57) to calculate cells/square, then in the end it would make sense to take the final number and say that 94.7% of those cells are viable.
Thank you so much I have 4 hrs tp submit my r
lab report you saved me thank youuuuuuuuu❤❤❤❤❤❤❤❤❤❤
excellent!! today I got the total process of how to calculate cells, thank you Miss for your wonderful video
Thank you for saving me. No video could explain it better.
Wow, this was well put together. Great job!
Thank you for the help. This video has now made my understanding of Hemocytometers even greater and therefore has also made my day better.
I have Diagnostic Virology practicals in two hours. Thanks for the video
What crystal-clear instruction. Amazing!
Wonderfully articulated and easy to understand, better than many of my teachers.
where did the 10^4 come from???
Even though it has been 7 years, the 10^4 comes from volume of suspension in each square (which is 10^-4 mL)
@@jelle902 why the calculation state that is 10^4? why not 10^(-4)
@@mjunchai630 so before you reached that step, it was calculating the concentration of cells in a square (which as he said is 10^-4 ml). We want to express our concentration in terms of cells per ml though, so we multiply by 10^4, essentially doing unit conversion.
@@jacobjackson3372 i dont get it can u elaborate it more pls
@@alexaoctaviano5038actually, this is because we convert microliters to mililitetrs:)
Thank you very much, now am ready for lab class tomorrow here at MD anderson
Hey it's been 6 years and I've always wanted to learn at MD Anderson. What are you doing now?
This is the best video about the topi... so easy to understand :) Thank you so much!
WOOOOW, It's super clear!, Thank you very much for your support!
This helped so much! I can thank you enough! Crystal clear! Keep up the great work:D
Crystal clear? Why non-viable cells look darker than their environment?
Really enjoyed it! Excellent quality throughout! Thank you so much! xoxo
This was the best video ever
Thank you so much. This video is a very interesting and very important video.
Good Video!
I have similar question. Should it be 10^3 (the factor that converts ul to ml)?
U will get the same answer whether u convert ul to ml because both the numbers having same units.....
Really easy to understand and great use of simple graphics, I'm just looking into yeast counts for brewing. If I can follow this anyone can, thank you.
Very helpful video....👍
Super clear explanations.Thankyou.
Hello in 4:01 why is it that the cell in the 3rd position on the very last row was not included in the count? Thank you😅
when to use trypan blue and when to use RBC diluting solution ?
Thank you perfect video and good eexplanation all my best
Thankyou so much for this amaaaaaazing vedio👍👍👏👏
Thank you, this is the best presentation in this regards
Thank you ver much. this video is very helpful.
How much microlitre we should put on one side?
thanks for this vedio because it is very helpful for making dilution
Mam can you explain if the RBC count is more in corner than centre so we only count center's RBC and not corner then report me make has cause an errors to diagnose patient 🤔🤔🤔🤔🤔🤔🤔🤔. Please tell about this
How much volume of cells plus trypan blue are you adding to the cytometer chamber
But when we do subculture how much amount of media or cell suspension will be required ..please explain that one also...and thanks for your good explanation
Why do they multiply by 10^4 when calculating for the concentration of viable cells/ml?
Because she counts number of cells per ml and cells she has count was in 0,1 microl, I guess
The reason you multiply by 10,000 is because each square contains 0.1 uL, so x 10^4 converts 0.1 uL to 1 mL
thank you soooo much for the great, smooth explanation
Amazing explanation
This helped me understand the technique really well..Thank you ❤️
Why non-viable cells look darker than their environment?
Thank you. This is just what I needed.
Thanks for such a great explanation ...really worthy enough
Why does it have 2 chamber but counted only 1 chamber? So what's about the other one?
You can for example count two different types of cells. In this case they only had one cell line, so no need to use the other side =)
very clear and easy to understand.
Thank you so much. This is really helpful.
Is 10^4 is the depth factor??
At calculating the cell number
Anthor question ,I know that if we have small cells(bacteria) we will count at the central square only and if it large cells(fungi) we will count the four squares on corners only, is that true?
Nicely presented.
hi question! if they so much as touch the bottom and right one, are they already excluded from the count? like if they touch the first line of the bottom and right lines. thank you!
Thanks that really would help alot.. what a simple yet great explanation 👌👌
Bro can you tell me from where the 10 raise to power 4 came...
@@The_UNKNOWN_MAN_777 I found the video very helpful but I also wanna know that too
Very clear, very useful. Thank you :)
We use this video in an introductory cell biology lab course, it's very informative and helpful! However, the linked portion where you can print off a certificate at the end appears to be broken. This has been a great mechanism for giving students credit for watching the video. Do you have any plans to make that part active again? Cheers!
Ahoy! Apparently there's no way to tag a channel in here nowadays and therefore no way that I'm aware of you get your attention, BioNetwork. So I'll just reply to my own comment and see what happens. Please take a moment to address the concern in my above comment. Thanks!
@@nathanstephens9442 email?
Well explained video and interesting. Truly helpful. Thank you!
Glad it was helpful!
A question please why was the concentration mulitplied by 10^4
The background music just gives it a 😌 vibe
Thankyou for this informative video. Appreciate it.
Really informative video! Although I understand the calculation for the percentage viability, I'm just wondering why the average number of cells per square (10.8) isn't rounded down; as it is not possible to have 0.8 of a cell? Thanks!
Ultimately, the purpose of the calculation is to determine the concentration of cells. It is true that it is not really possible to have 0.8 of a cell. However, the exact number, 10.8, is used in the calculation for the concentration of cells for a more accurate result. Hope this helps!
How we take into account the original volume of cell suspension?
very good presentation
Amazing video indeed. can somebody please tell me about which tryphan blue solution is used i=here. can I use tryphan blue 1 % in dH2O. Need help. Thanks
So what's the normal range for WBC using hemocytometer?
Why fill both squares? You've only counted one.
Good question!
it to work as a duplicate and increase accuracy but she had forgotten to do so while putting an unnecessary google
Nothing about creating newtons rings undercover slip for accurate counting .?
any vedio about conidial production and measurement of vegetative growth of fungi
thanks for the great video:) I wanna ask, is there any other alternatives for trypan blue? like common stains that easy to prepare or obtain? can methylene blue be used instead of trypan blue?
Great question!
Methylene Blue is unsuitable for cell viability as it will stain both live and dead cells equally. You can try automated counters, fluorescent dyes (propidium iodide), or colorimetric assays (MTT, MTS, WST-1) instead. However, all these methods will require you to have specialized equipment to test the viability of cells. Trypan blue is a much cheaper alternative.
@@kosheeka thank you so much for the answer🤧
Is there a specific type of pipette you have to use?
Which microscope you used for this experiment? kindly let me know
Can anybody please explain, I want to count cells with computer by taking an image of the simple and I need to know what chamber one uses to do this?
Am I correct in my understanding that I require a counting chamber but not a hemocytometer??
When I look the term counting chamber it keeps comming up with a hemocytometer but I don't desire to manually count cells.
ua-cam.com/video/-F3PZj9zo-U/v-deo.html
Is there a chamber not a hemocytometer to hold a known stained sample to photograph
I have also seen Hemocytometer spelt Haemocytomer and Hemacytometer. Can anyone please confirm the correct spelling? Thanks
Hi
Is this suitable for sample has oil??
And the trypan blue solution is suitable for sample has oil??
thanks you for such a beautiful video , its an interesting task.
Can you tell me, please, what model of microscope do you use in this video?
This is very nice. Thank you
Glad you like it!
I love it...Good refresher.
what is the publication where did you get these calculations? it should be in the description. Thank you
Well explained.
We glad it was helpful for you.
Everytime we should count the cells in the five squares or it is the experimentalist's choice ?? Please reply.
Can I use hemocytometer while counting a bacterium
Thank you so much!! This is very helpful!!
please where are you doing this manip? is it possible to realise a work there...i'm looking for a cell culture lab for an internship.
for cyanobacteria or blue gree algae also the dead cell appear blue?
thank you so much... its really awesome...
Is that a standard light microscope?
In my readings it says that a fluorescent microscope is required to detect the dye that distinguishes living from dead cells.
She used Trypan Blue. It is used with a light microscope.
How to count sel density microalgae that has spiral form like Spirulina with haemocytometer?
The virtual hemocytometer isn't accessible :((
Try ncbionetwork. org/iet/hemocytometer/
Thank you, Finally I understand it
Does anyone know why it is multiplied by 10^4 ??
Irram Sanna Because each large square houses 10^-4 mL of liquid. You multiply by 10^4 to find the number of cells that would be in a whole milliliter of liquid. This makes your units: cells/mL
Daniel Bundrick Thanks for that response. I was wondering that too and no one at work could tell me.
Easy to understand, Thanks
Great explanation !!!
how would you work out number of cells per ul ?
+Sam O'Brien You take the number of cells per milimeter and divide it by 1000
How do you prepare the cell suspension?
How do you know how many volume to seed?
I don't get that you've used average # of viable cells/square to measure the concentration and at last, you mention that 94.7% of that concentration are viable. Shouldn't you be using total # of cells per square and then use it to determine total concentration, of which you'll have 94.7% viable cells concentration?
How much grams of trypan blue in how much ml of distilled water you have taken
why multiply by 10000 at the end? You never explain that.
it's to change mikroliters to mililiters
@@mateuszklimczewski1881 the conversion for uL --> mL is 10^3, not 10^4
how does doing this tells me if there is a certain disease? like what is the average number of cells that i should be comparing my results to ?
no place
Very nice 👍
Great video!
it was 10^-4 suspension (negative) meaning 1ul right? so 10.8*2*1000ul =21600 why do we multiply with 10^4?
I get it 0.1ul i said it was 1ul so I should multiply by 10 so the yare 216/ul
One large square (or with depth cube) has a volume of 0.1 µL. Since you want to calculate the concentration of cells per mL (1 mL is 10000 times more than 0.1 µL) you multiply the number of cells per large square (10.8) by a factor of 10000 (10^4) to get to the number of cells per mL. You also multiply by 2 because you diluted the sample with the dye.10.8 * 10^4 * 2 = 2.16 * 10^5 cells/mLor10.8 * 10000 * 2 = 216000 cells/mL