Plasmid DNA Extraction (Traditional Alkaline Lysis Method)

[@DJmisterpeluca]

Super helpful, you're supposed to do all these steps to separate the DNA from certain bacterial contents like proteins and lipids, all these make a lot more sense now, at the end we precipate the DNA with the alcohols and then dilute them for further analisis in the TE solution.
Thank you very much.

[@nahidislam2753]

Thanks.This video is very informative and every steps is shown easily , we want to know procedure of plasmid curing.

[@N00bzDown]

Thanks a lot I really find this helpful

[@davidzinn7227]

I was in the middle of doing homework and saw this, now i'm putting it off and going to finish watching Code Geass. Lelouch vi Britannia commands me.

[@Scriabin_fan]

I got to the isopropanol step and centrifuged it, when I took it out of the centrifuge I couldn't see a pellet. I think I might've left it too long. So if I centrifuge again could i potentially Iget a pellet?

[@baharataei1126]

Please also indicate the temperature of the centrifuge

[@mullainathanm8381]

4 degree C

[@mizahirhussain]

good one

[@ankitayadav1123]

Thanks it is useful 😀

[@abhisekhansda2806]

What is TB?

[@gauster8]

Bata deta Bhai pr ab 2 saal ho gye tere question ko 🤡

[@abhisekhansda2806]

@@gauster8 han to abhi bata dega to kya dikkat hojayega dost🤡

[@gauster8]

@@abhisekhansda2806 bhai tris buffer naam ka buffer hota h, waise abhi kya padh raha h🤡

[@Rvhddbjredhh]

what are the observations after adding solution 2?

[@حسنحیدرزادگان]

خب بابا میدونیم میدونید بیدارم زنگ زدن نمیخواهد که

[@shraddhasuryavanshi4364]

worst protocol ever,worst time management..i isolate around 10 plasmids in this mch time and if you use RNAse at the start you must use a co-precicitant...shit protocol..

[@beningham9782]

They're showing how to do it, they're not going to be working at their usual speed

[@safwanarif_mehmood11]

Could u please share wid me a protocol of plasmid extraction