DNA Extraction by Phenol Chloroform

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  • Опубліковано 23 гру 2024

КОМЕНТАРІ • 59

  • @ladushky1
    @ladushky1 3 роки тому +13

    Thank you so much! This video remains super helpful after 10 years!

  • @gdastray
    @gdastray 11 років тому +7

    not sure if you already found out. IIRC phenol is a bit soluble in water. chloroform prevents that. and isoamyl alcohol prevents bubbling, which i'm not so sure what that will affect. perhaps visibility. using phenol n chloroform alone would do in ratio 1:1. repetition needed to make sure you have removed as much as protein. usually people do 3 cycles of purification before precipitating DNA

  • @macmichaelrubio2578
    @macmichaelrubio2578 7 років тому +13

    I have a little question. Why are you transferring Phenol:Chloroform:Isoamyl in an open area. This solution is toxic and carcinogenic so it must be done under fumehood. Thanks.

    • @baraiyakartik7594
      @baraiyakartik7594 6 років тому

      Right

    • @j.5908
      @j.5908 5 років тому +1

      It's indian style...what can I say..

    • @keerthana7353
      @keerthana7353 5 років тому +1

      Jit Goria lol this is an instructional video and to me pretty much looks like it’s done safely. You really can’t see the background or anything because everything is done close up. Just like most other instructional videos made by companies out there.

  • @drsamehgene
    @drsamehgene 8 років тому +21

    A very good movie, however, all mixing steps were performed by pipetting and this could be problematic for DNA sharing or damage as we know that gDNA is a fragile component. I prefer the up side down method to avoid such sharing.

  • @francoveloso4054
    @francoveloso4054 Рік тому

    How much volume do you use to resuspend DNA in TE buffer?

  • @kimanhnguyen2984
    @kimanhnguyen2984 2 роки тому

    Can I ask something? What is the lysis buffer recipe and can the DNA from this protocol be used for PCR with the precise result?

  • @lekiningroydann4237
    @lekiningroydann4237 Рік тому

    What is the temperature for centrifugation?

  • @Strictlymusicworldwide
    @Strictlymusicworldwide 2 роки тому

    hey can you please recommend me 10 books on this, im doing practical and google is not helpong

  • @seeratfatima9910
    @seeratfatima9910 7 років тому

    Is it a technique for any microorganism's DNA isolation???

  • @bepositive5181
    @bepositive5181 9 років тому +2

    Onur Kerem Polat. You are my good friend and I am always respect this friendship.haha

  • @zl7650
    @zl7650 5 років тому

    What is the purpose of adding chloroform into the last step?

  • @sujanapokharel5555
    @sujanapokharel5555 6 років тому +1

    I've tried to extract s.aureus dna from wound samples for more than 3 times but my test is negative😢 whats the reason behind failure

    • @muneebakhan7237
      @muneebakhan7237 6 років тому

      what method are you using for s.aureus , please tell me or give me alink . i wiill be grateful

    • @anshumansahu1087
      @anshumansahu1087 6 років тому

      This is because gram +ve bacteria have thick cell walls and hence are more difficult to deal with as compared to gram -ve bacteria. I am dealing with the very same problem.

  • @charleszhou5021
    @charleszhou5021 4 роки тому

    Great video, you may use guanidine thiocyanate is used for RNA isolation and can solubilize proteins.

  • @superbscientist743
    @superbscientist743 5 років тому

    Why does the DNA sticks to the side wall of eppendorf tube at the last step of centrifugation ?

    • @ronaldmacdonald1268
      @ronaldmacdonald1268 5 років тому +2

      Because the eppendorf while centrifugation won't reach an horizontal state. The angle is locked so that it doesn't reach that state.
      Meanwhile the acceleration vector is horizontal(because of rotation) , therefore your DNA will stack up on the side of your tube. This way it will tend to "glue" to the tube once precipitated instead of flowing with the sumernatant while you eliminate it.
      Sorry for bad English.

    • @superbscientist743
      @superbscientist743 5 років тому +2

      @@ronaldmacdonald1268 Thank you so much. And yes the English is comprehensible. No sorry please.

  • @arifshah2115
    @arifshah2115 Рік тому

    You should also mention the reasons that why,for what purpose we use particular chemicals,.. Otherwise great tutorial.

  • @kimconguyen913
    @kimconguyen913 9 років тому

    Thank you for this video, it is so nice. But I confuse is this genomic DNA or plasmid isolation and how to make lysis buffer?

  • @peerzadazubairahmad464
    @peerzadazubairahmad464 4 роки тому

    One of the best tutorial.thanks
    👍👍👍👍👍👍👍👍👍👍👍

  • @matthewbaltuskonis2549
    @matthewbaltuskonis2549 2 роки тому

    People still do this?

  • @preetikapoor1877
    @preetikapoor1877 8 років тому

    can we change the ratio of PCI????

  • @ikmalalif3259
    @ikmalalif3259 7 років тому

    what is the function of Proteinase K in this experiment?

    • @piecemaker74
      @piecemaker74 7 років тому +4

      ikmal alif this is a protease which will denature protein so that you do not get any in your lysate. Likewise, RNAse will degrade RNA.

  • @cookyday923
    @cookyday923 6 років тому

    اي بعدين هسة شتردون ..مو اضافات طلاسم ..يليتكم طقعتو بس

  • @nareshbarik5384
    @nareshbarik5384 9 місяців тому

    Thank you 🙏

  • @hind9705
    @hind9705 2 роки тому

    Great video... It helps me a lot

  • @SaraKhan-wq2pt
    @SaraKhan-wq2pt 5 років тому +1

    Good job!

  • @laudeabelouakou407
    @laudeabelouakou407 12 років тому

    I want to doing this experience

  • @dharmarajthapa974
    @dharmarajthapa974 10 років тому +1

    Thanks a lot.

  • @BitsdeCiencia
    @BitsdeCiencia 9 років тому +1

    muy bueno. Me suscribo

  • @samanthabyrne2970
    @samanthabyrne2970 11 років тому

    Excellent,thanks a mill

  • @drkinans
    @drkinans 11 років тому

    Very good, deep thanks

  • @الحمدللهربالعالمين-ي6د

    Muchas gracias desde chile

  • @ajp3912
    @ajp3912 5 років тому

    Wow, that was very tedious

  • @houndguys
    @houndguys 13 років тому

    This is cool!!!!!

  • @sanjithsh
    @sanjithsh 6 років тому

    Sry..Its really amazing

  • @magaliemorin9402
    @magaliemorin9402 2 роки тому

    Bio mol rosemont manifestez vous

  • @sanjithsh
    @sanjithsh 6 років тому

    Very poor

    • @husseinabdullahi4312
      @husseinabdullahi4312 5 років тому

      I have question.
      As we generally know some reagents sometimes nin reactibity so how could you decide that your performance is correct?