Розмір відео: 1280 X 720853 X 480640 X 360
Показувати елементи керування програвачем
Автоматичне відтворення
Автоповтор
What is your sample volume?
Very good video, can u tell me that how much is the sample of standard protein serial dilutions
How we must apply sampel for 200uL reagen Bradford?
Now that some intense background music.
Shouldn't the standard curve start from the origin, once you substracted the blank from each of your measured absorbances?
Don´t you need to subtract the blank absorbance value of the absorbance of the samples?
Hi Euder,The preparation of standard curve has included 0 ug/mL titration which is the blank value.
For serial dilution wht solvent u have used??
what is the blank in sample measurment?
Do you have to pipette the 5x reagent under a fuming hood? My co-workers insist I have to do it under the hood...
Pussies, it doesnt fume
hi..can u explain what the objective that the protein assay by biuret n folin please..i understand this one its just those...
can I use Coomassie Briliant Blue R250 instead? If yes, should I dilute it to 1:5 also? or I can use as working reagent directly?
nice video but why you have use mg instead of ml hence your standard is in liquid form?
The sample was from from 10mg/ml bsa stock. So they only dilute the stock to desired concentration.
would be good to know the volumes of the standards and samples added...
Yeah... But in biorad protocol they suggest for microtiter plate method, they use 250ul of 1x bradford dye, while samples/ standard only 5ul
I give up. Not a single protocol in the world is detailed enough. Insane that no one provides this information! Unreal!
Awesome! THanks for the review !! 🤘🏽🙏🏽🔥
What is your sample volume?
Very good video, can u tell me that how much is the sample of standard protein serial dilutions
How we must apply sampel for 200uL reagen Bradford?
Now that some intense background music.
Shouldn't the standard curve start from the origin, once you substracted the blank from each of your measured absorbances?
Don´t you need to subtract the blank absorbance value of the absorbance of the samples?
Hi Euder,
The preparation of standard curve has included 0 ug/mL titration which is the blank value.
For serial dilution wht solvent u have used??
what is the blank in sample measurment?
Do you have to pipette the 5x reagent under a fuming hood? My co-workers insist I have to do it under the hood...
Pussies, it doesnt fume
hi..can u explain what the objective that the protein assay by biuret n folin please..i understand this one its just those...
can I use Coomassie Briliant Blue R250 instead? If yes, should I dilute it to 1:5 also? or I can use as working reagent directly?
nice video but why you have use mg instead of ml hence your standard is in liquid form?
The sample was from from 10mg/ml bsa stock. So they only dilute the stock to desired concentration.
would be good to know the volumes of the standards and samples added...
Yeah... But in biorad protocol they suggest for microtiter plate method, they use 250ul of 1x bradford dye, while samples/ standard only 5ul
I give up. Not a single protocol in the world is detailed enough. Insane that no one provides this information! Unreal!
Awesome! THanks for the review !! 🤘🏽🙏🏽🔥