Not often does university level educational content come at this of a high quality and absolutely free. The camera work is immaculate, the sound design relaxing, and the storyboarding ingenious. Biochemistry is one of my favorite disciplines of science and I am sad that not many people will see this masterpiece of modern education. Wish you the best
Varies depending on the kit you buy. Should be the same volume of Bradford reagent in every tube, because the variable you are changing is protein concentration
Great video. 😮 Sir, what could have gone wrong if blank (control) has a higher reading (O.D.) value than the standard but they have same color visually.
Not often does university level educational content come at this of a high quality and absolutely free. The camera work is immaculate, the sound design relaxing, and the storyboarding ingenious. Biochemistry is one of my favorite disciplines of science and I am sad that not many people will see this masterpiece of modern education. Wish you the best
Thanks for taking the time to comment! Science is not what people relax to on UA-cam, still finding the best format. More to come soon
Great video! Really helped me with my lab report : )
Great to hear - thanks for watching
A very helpful explanation, would you be able to talk about the Modified Lowry Method Assay and procedure?
Very clear. Thanks.
How much of Bradford reagent should we add?
Varies depending on the kit you buy. Should be the same volume of Bradford reagent in every tube, because the variable you are changing is protein concentration
How do I decide the concentration of protein I should load per well when running my western blot? Help!
Great video. 😮 Sir, what could have gone wrong if blank (control) has a higher reading (O.D.) value than the standard but they have same color visually.
Your blank has been contaminated? The reading should be a lot lower
Sorry ! Is there some software or algorithm for drawing the standard curve ?
Nothing fancy, just Microsoft excel or you can draw it by hand.
@@BioLabCollective Thanks very much
My concentration is 0.3952mg/ml how can its calculate its volume to load maximum 50ug of protein per well?
We go into this topic here: Laboratory Solutions and Dilutions
ua-cam.com/video/t2eC_hvaIBc/v-deo.html
concentration = mass / volume
good vid but you did too much cutting while edditing, it seems too fast to understand
Thanks for the feedback. Unfortunately slow videos have low watch time and the algorithm downvotes them on UA-cam. Will try to make every video better