Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)

References/Resources: / the_crux
Mitochondrial DNA is replicated independently of the nuclear DNA. But mitochondria depends on the proteins made from the information in nuclear DNA. It replicates via D loop formation through a strand displacement mechanism initiated at OriH. The helicase TWINKLE and DNA Polymerase Gamma drive the initiation of the major arc, which activates the OriL through a stem loop structure. Finally, DNA polymerase circles back to the origin and terminates replication. While this sounds simple, many aspects of the mtDNA replication are not clearly understood - this video discussion also highlights the many gaps in our knowledge concerning mtDNA replication.
Jump to your favorite section:
00:00 mtDNA structure
02:43 mtDNA enzymes
04:37 mtDNA replication
05:58 OriH initiation
09:42 D-loop regulation
11:44 OriL initiation
14:22 OriL termination
15:19 OriH termination
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[@adeeba6945]

It was so precisely explained on books that I couldn't understand it well .....thank you so much for making it clear and covering every point of this topic!

[@dewanchettri2580]

Covered every aspect to make it understandable, and worth watching it. 😊
Thanks.

[@kanchanmukherjee2167]

It was really good. You explained it from every inch. Thank you. I request you to upload a video on "mitochondrial transcription and translation" ... This will also be helpful for our easy understanding.
Thank you again and waiting for your next vdo on mtDNA transcription.

[@lipipragyadevi]

Very well explained , I literally searched in so many books but didn’t find proper explanation. Can you tell me your reference book though ?😊

[@mansikesarwani900]

Best explanation sir.

[@anushmitapaul4650]

Make a video on mitochondrial transcription

[@osamaalghatarifi2863]

You are explaining so fast !

[@vpdibin6553]

Thank you. 🥺🙏🙏🙏🙏

[@dewanchettri2580]

🙏

[@KarenKarimi]

Wow.

[@anands1508]

Sir, Am confused about the inner and outer strand .In many books, the inner strand is heavy(H) and outer (L).So could you please exaplain?

[@theCrux]

The way you draw inner and outer does not matter - there is no such thing as inner and outer as it means nothing. H and L carry meaning because it represents the base information. As long as you follow H and L, you are good! You can flip H and L to inner and outer (and vice verse) - just make sure to keep the direction of promoters consistent.

[@abineshanesha9141]

What does the red colour indicate???

[@theCrux]

In this video, the color scheme does not have any particular meaning.