May I ask a doubt?in case of phix174 why does the helicase/Dnab even assemble on to a single strand?is it because the primase can only assemble if helicase is present?
They assemble because of the primosome (Pri A B C) proteins. DnaT also aids in this assembly. Otherwise Helicase/DnaB/Primase would not bind the X174 ssDNA. For the assembly and interaction between primase and helicase, I suggest you take a look at Prokaryotic initiation assembly - there is more detail in the video than I can explain in the comment :) Playlist: ua-cam.com/play/PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7.html
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Just Awesome, from start to end everything with in detail and perfection.
sir plz made more videos because your way of explaining and data best
Thanks dear thecrux for this video... Big fan ....
Thank you so much ❤
Excellent presentation..could you please share the reference text if possible.
May I ask a doubt?in case of phix174 why does the helicase/Dnab even assemble on to a single strand?is it because the primase can only assemble if helicase is present?
They assemble because of the primosome (Pri A B C) proteins. DnaT also aids in this assembly. Otherwise Helicase/DnaB/Primase would not bind the X174 ssDNA. For the assembly and interaction between primase and helicase, I suggest you take a look at Prokaryotic initiation assembly - there is more detail in the video than I can explain in the comment :)
Playlist: ua-cam.com/play/PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7.html
Thank you 😊