theCrux
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Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]
References/Resources: www.patreon.com/the_Crux
All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html
This video discusses the regulation of protein expression using a tetracycline inducible or repressible based system i.e. the tet-ON and tet-OFF system. The control is based on the repressor (tetR) from the tet operon and its fusion with a viral protein (VP16) activation domain. This forms a transactivator, used in the tet-OFF system - its basis is allosteric inhibition. For tet-ON a mutated version of tetR, called reverse tTA (rtTA), is used - its basis is allosteric activation. Both systems use a special promoter called TRE - Tetracycline responsive element, which contains a minimal promoter and tandemly repeated tet operator. I discuss the general concept behind the regulators (by taking the example of Takara Bio's commercially available Tet-based kits: Tet-ONE, Tet-OFF 3G, and Tet-ON 3G which includes TREG3G) and also some concerns that arise when using the tet based system.
00:00 Tet Operon basics
01:40 tetR usage
02:18 VP16 activation domain
03:30 tTA (tetR + VP16)
04:26 Tet-OFF system
05:55 Use
06:47 Squelching
07:32 rtTA and Tet-ON
References/Resources: www.patreon.com/the_Crux
Suggested videos:
Playlist on Operons: ua-cam.com/play/PL0Ymnd-zt4Ij4jwm3B8TG7tXeby6MQYaR.html
Protein Expression Vectors [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html
Protein Expression Vectors [Part 2]: ua-cam.com/video/x3xhV2ZZZpE/v-deo.html
Protein Expression Vectors [Part 3]: ua-cam.com/video/BhKzoIUH9_Q/v-deo.html
Protein Expression Vectors [Part 4]: ua-cam.com/video/JHRIA8v40qA/v-deo.html
*Must watch playlists*
All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html
All videos on DNA Replication: ua-cam.com/play/PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7.html
All videos on Transcription: ua-cam.com/play/PL0Ymnd-zt4Ij2VcAOHNUweftSElVsc-MX.html
All videos on Translation: ua-cam.com/play/PL0Ymnd-zt4IjAAAMlPLDIXbs692Yp14xa.html
Consider supporting the growth of this channel by becoming a Patron: www.patreon.com/the_Crux
I appreciate your support :)
Переглядів: 120

Відео

Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]
Переглядів 18421 годину тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html This video discusses the regulation of protein expression vectors by promoters and operators. Specifically, we discuss why regulation is important and how it impacts protein yields, the different types of regulated promoters and how they work. Simple regulati...
Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]
Переглядів 28814 днів тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html In this video, we explore some common protein expression vector designs and how they are constructed. These are helpful examples and applications of protein expression vectors to intuitively understand the protein expression vector construction. We discuss 5 ...
Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]
Переглядів 24621 день тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html In this video, we explore how to obtain templates for protein expression vectors depending on the gene structure (intron and exon language). Prokaryotic genes are intron-less, whereas eukaryotic genes often have introns. Obtaining template for protein express...
Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]
Переглядів 22328 днів тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html Protein Expression vectors are used to make protein from the template DNA. In this video, we discuss some of the common applications of protein expression vectors and the complications involved with protein expression when changing the host i.e. heterologous ...
RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)
Переглядів 221Місяць тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html RNA Expression vectors (different from protein expression vectors) are used for the sole purpose of making RNA from a template DNA. In this video, we discuss some of the common applications of RNA expression vectors and their rules/principles of design. Final...
Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning
Переглядів 1,1 тис.3 місяці тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html Golden Gate Cloning is a restriction enzyme based cloning that uses type IIS restriction enzymes. It is a powerful way to clone large numbers of inserts in a single reaction without the need of using multiple enzymes. This video discusses the basis of golden ...
Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning
Переглядів 1,4 тис.5 місяців тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html Gateway Cloning is a popular recombination based cloning method. It does not rely on restriction enzymes but instead uses integrases to recombine att sites between a final and donor vector. This causes an exchange of DNA segments that inserts the desired DNA ...
TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional
Переглядів 3,2 тис.8 місяців тому
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html TOPO Cloning (Topoisomerase-based cloning) is a commonly used method to clone PCR amplicons. TOPO Cloning bypasses the need of restriction enzymes and ligases. In this video, we discuss the three main types of TOPO-cloning systems - TOPO Blunt, TOPO TA, and T...
Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
Переглядів 1,1 тис.9 місяців тому
References/Resources: www.patreon.com/the_Crux Playlist - Genetic Engineering: ua-cam.com/play/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf.html Molecular Cloning with restriction enzymes can be very easy or very challenging. This video discusses the fundamental considerations of traditional restriction enzyme based molecular cloning. We discuss sticky and blunt ends, the process of blunting, and how all...
Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer
Переглядів 3,5 тис.9 місяців тому
References/Resources: www.patreon.com/the_Crux This video discusses the plant cloning vectors focusing on Agrobacterium Tumefaciens based vectors and its dependency on the natural infection mechanism. This involves understanding Ti plasmids, disarmed Ti vectors, cointegrate vectors, and binary vectors. The process of cloning in plants uses conjugative transfer of T DNA through conjugation betwe...
Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
Переглядів 4,4 тис.10 місяців тому
References/Resources: www.patreon.com/the_Crux This video discusses the general biology of yeast cloning vectors. There are 5 classic vectors - Integrating, Episomal, Replicating, Centromeric, and Artificial Chromosomes - as well as the naturally occurring Yeast 2 mu plasmid. Yeast vectors rely on coli-based vectors like pBR322, pUC19, or Bluescripts. Typically, yeast cloning vectors tend to be...
P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality
Переглядів 1,7 тис.10 місяців тому
References/Resources: www.patreon.com/the_Crux This video discusses the general biology of P1 bacteriophage based cloning vectors (pNS582 and pAd10SacBII) and P1 derived artificial chromosomes (PACs - pCYPAC1). The discussion walks you through the development of the P1 system with its use of Pac site and Cre-loxP recombination, coupled with SacB driven synthetic lethality. The main focus of the...
F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)
Переглядів 1,3 тис.10 місяців тому
References/Resources: www.patreon.com/the_Crux This video discusses the general biology of F plasmids, Bacterial Artificial Chromosomes, BACs, and Fosmids. We start the origin of high capacity cloning vectors with the F plasmids and the development of mini F vectors. This was the inspiration for BACs which went through a few iterations involving retrofits and screening markers. Fosmids were dev...
Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus
Переглядів 5 тис.11 місяців тому
References/Resources: www.patreon.com/the_Crux This video discusses the general biology of lambda bacteriophage vector and cosmid cloning vectors. There are two types - insertional and replacement - of vectors derived from lambda bacteriophage. This requires the use of spi phenotype since lambda phage vectors have no selection or screening markers. From the lambda bacteriophage vector, cosmids ...
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
Переглядів 3,4 тис.11 місяців тому
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
Переглядів 1,6 тис.11 місяців тому
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
Restriction Enzymes in Molecular Cloning
Переглядів 1,5 тис.11 місяців тому
Restriction Enzymes in Molecular Cloning
Genetic Engineering - Classic vs Modern methods
Переглядів 2,7 тис.11 місяців тому
Genetic Engineering - Classic vs Modern methods
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
Переглядів 3,5 тис.Рік тому
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
Переглядів 2,1 тис.Рік тому
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)
Переглядів 10 тис.Рік тому
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 PhiX174)
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
Переглядів 11 тис.Рік тому
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
Переглядів 4,9 тис.Рік тому
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
DNA Replication in Space and Time
Переглядів 559Рік тому
DNA Replication in Space and Time
Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation
Переглядів 1,6 тис.Рік тому
Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation
DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism
Переглядів 2,4 тис.Рік тому
DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism
DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase
Переглядів 5 тис.Рік тому
DNA Replication (Eukaryote) ELONGATION - Polymerase Switching different types of DNA polymerase
DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome
Переглядів 2,3 тис.Рік тому
DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome
DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer
Переглядів 3 тис.Рік тому
DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer

КОМЕНТАРІ

  • @elijahjohn4482
    @elijahjohn4482 5 годин тому

    Is it really true though that prokaryotic dna is not associated with proteins? That's a historical misconception isn't it? Just 2 months ago I learned about H-NS. (Not trying to be a smart-ass. Love your channel <3)

    • @theCrux
      @theCrux 4 години тому

      True, prokaryotic DNA is not naked DNA. It does have a lot of proteins associated with it - just not the same level of compaction provided by associated structural organizers we see in eukaryotes. H-NS, NAPs, HUs, FIS and many more are great examples of prokaryotic DNA organizers. I should have been more careful with my choice of words (at the cost of over-simplification) in this video. Thanks for pointing it out!

  • @Rajat_1010
    @Rajat_1010 17 годин тому

    Dhanyavaad!! So glad you uploaded this!! It was really helpful!!

  • @alekoukassiaropoulos382
    @alekoukassiaropoulos382 День тому

    I really love your videos but I would like to ask if you could tell me where you find your information from? I can't seem to find similar information as you, so it really bothers me.

  • @theCrux
    @theCrux 5 днів тому

    Protein Expression Vectors [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html Protein Expression Vectors [Part 2]: ua-cam.com/video/x3xhV2ZZZpE/v-deo.html Protein Expression Vectors [Part 3]: ua-cam.com/video/BhKzoIUH9_Q/v-deo.html Protein Expression Vectors [Part 4]: ua-cam.com/video/JHRIA8v40qA/v-deo.html

  • @mimous54mim44
    @mimous54mim44 8 днів тому

    Excellent video

  • @samirandas4456
    @samirandas4456 9 днів тому

    Sir can you make a full playlist of cell and microbiology and genetic?

    • @theCrux
      @theCrux 9 днів тому

      Yes, it is part of my future plan :)

  • @ankitasarkar9316
    @ankitasarkar9316 9 днів тому

    Thank you! I look forward to your videos. They're of great help!

  • @mobagamer69
    @mobagamer69 9 днів тому

    Thank you

  • @theCrux
    @theCrux 9 днів тому

    Protein Expression Vectors [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html Protein Expression Vectors [Part 2]: ua-cam.com/video/x3xhV2ZZZpE/v-deo.html Protein Expression Vectors [Part 3]: ua-cam.com/video/BhKzoIUH9_Q/v-deo.html Protein Expression Vectors [Part 5]: ua-cam.com/video/1PtIdAQoqME/v-deo.html

  • @mobagamer69
    @mobagamer69 10 днів тому

    Thank your for the video, highly informative. Can you please make an video on genetic mutations. Your videos are perfect and very informative.

  • @KeisyaDhiandraPrabowo
    @KeisyaDhiandraPrabowo 13 днів тому

    VERY HELPFULLL, THANKYOU!

  • @thesublimeu1532
    @thesublimeu1532 15 днів тому

    how is the araC gene interrupted by the o2 operator in between? shouldn't the orf of a gene be continuous?

    • @theCrux
      @theCrux 15 днів тому

      araC gene i.e. CDS (not drawn for most of the video but see 9:42) is downstream of the operator. The O2 does not interrupt the CDS; the operator is between araC promoter and the araC CDS.

    • @thesublimeu1532
      @thesublimeu1532 15 днів тому

      @@theCrux oh! That makes it clear, thank you so much!

  • @Fox064
    @Fox064 15 днів тому

    Is there a risk that concatenating proteins (even with a linker) can cause misfolding? And if not is it because the ends of proteins don't typically contain functional domains and are more flexible with a less stable conformation and the second protein basically "just" hangs there? Anyway thank you for informative video!

    • @theCrux
      @theCrux 15 днів тому

      Yes, fusions even with a linker does not guarantee that partner proteins will work - it depends from protein to protein. In case the partner protein(s) is misfolded (regardless of fusion) then yes, it just hangs there. Chemokines for instance are not amenable to N-terminal engineering (even a single amino acid extension can be bad). Mini-motifs are generally found in C-terminal ends. C-terminus also gets a lot of PTM because of solubility and accessibility. I am not sure if I would say it does not contain "functional domains". The C-terminal is usually the exposed end (disordered and solvent accessible) so more often than not, it tolerates linkers well (but not always). Unless you have a specific reason to keep the partner proteins "stuck together" (say for single-molecule imaging, protein tracking, etc.) you may consider adding a 2A-peptide before or after the linker. I may have alluded to 2As as bi-cistronic systems in the past video(s).

    • @Fox064
      @Fox064 15 днів тому

      @@theCrux Oh you are right I forget about things like C-terminal phosphorylation of RNApol II which they told us at molecular bio course and underestimated importance of the protein ends. Apparently I have a lot to refresh! Anyway thank you for the answer 😊.

  • @cenni1887
    @cenni1887 16 днів тому

    5:31 I see what you did there

  • @theCrux
    @theCrux 16 днів тому

    Protein Expression Vectors [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html Protein Expression Vectors [Part 2]: ua-cam.com/video/x3xhV2ZZZpE/v-deo.html Protein Expression Vectors [Part 4]: ua-cam.com/video/JHRIA8v40qA/v-deo.html Protein Expression Vectors [Part 5]: ua-cam.com/video/1PtIdAQoqME/v-deo.html COVID-19 mRNA vaccine biology: ua-cam.com/video/1FyogBhvsaI/v-deo.html

  • @theCrux
    @theCrux 16 днів тому

    Protein Expression Vector [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html Protein Expression Vector [Part 3]: ua-cam.com/video/BhKzoIUH9_Q/v-deo.html Protein Expression Vector [Part 4]: ua-cam.com/video/JHRIA8v40qA/v-deo.html Protein Expression Vector [Part 5]: ua-cam.com/video/1PtIdAQoqME/v-deo.html

  • @LionRonan123
    @LionRonan123 16 днів тому

    I have watched your series on Transcription and Genetic Engineering as well. I am so surprised that this level of quality and information is made free to us. Thank you! Your channel is a blesssing.

  • @ArtSthrills
    @ArtSthrills 19 днів тому

    13:16 it will be nuclear import right? So detailed and very clear vdo. I am watching all your vdos.

    • @theCrux
      @theCrux 19 днів тому

      Ah, yes, nice catch! You are correct, it will import into the nucleus.

  • @bappadityachandra10
    @bappadityachandra10 20 днів тому

    Please keep making such interesting videos.

  • @bappadityachandra10
    @bappadityachandra10 20 днів тому

    Thank you so much for this detailed explanations. It helps a lot to understand the process clearly.

  • @ciwslc
    @ciwslc 20 днів тому

    HI, thanks for another brilliant video. I still have one doubt around screening blunt ended inserts derived from Topo Blunt Cloning. Is the Colony PCR and Sanger seq the only way of screening for fragments inserted in the wrong direction?

    • @theCrux
      @theCrux 20 днів тому

      Restriction digest would be the first thing to try before going for Sanger Seq. Colony PCR works too, but it is prone to false positives given high sensitivity + it needs primers/PCR reaction; so it can get relatively expensive than the other two options.

  • @Rajat_1010
    @Rajat_1010 22 дні тому

    Thank you for uploading this!!

  • @ciwslc
    @ciwslc 22 дні тому

    Hi, thank you for your video, amazing explanation. I am curious about the screening methods that can be used to detect right orientation of the insert in the case of Topo blunt cloning. Is it done after transfection? what is the process?

    • @theCrux
      @theCrux 22 дні тому

      Do you mean Transformation (not transfection)? In that case, yes. You transform bacteria with the TOPO reaction, get colonies, get plasmids from a bunch of them and then screen for orientation. There are many ways to do so: Restriction digest, Sanger, PCR etc. This video on plasmids goes into screening methods: ua-cam.com/video/2pAM-FTFI2c/v-deo.html

    • @ciwslc
      @ciwslc 18 днів тому

      @@theCrux Thank you! I saw the video, another incredible one! So, for screening for right direction, Colony PCR follow by Sanger sequencing are a good approach?

    • @theCrux
      @theCrux 18 днів тому

      Yes, that seems reasonable (although most people would just stop after their colony PCR shows appropriate results; and Sanger only if they have a CDS and they care about ORFs/mutations etc.). More commonly, you would extract plasmid from a bunch of colonies (mini-prep) and then directly Sanger on the plasmid and/or do restriction digest (with this approach, colony PCR is not required). Colony PCR (followed by Sanger, if necessary) is a quicker alternative if you are screening for "many" colonies and therefore don't want to spend time/resources on mini-preps.

  • @twinrovas
    @twinrovas 23 дні тому

    i'm confused on how the overhangs are generated. with the BsaI example, how do you know exactly where the cuts occur? like with the lower strand, why does it cut after 5 nucleotides upstream of the recognition site while the upper strand gets cut at a different location? sorry if you answered this in the video, i'm just not following.

    • @theCrux
      @theCrux 23 дні тому

      BsaI is a Type IIS restriction enzyme. It is just how all Type IIS enzymes work (binding and cutting locations are not the same). The upper and lower strand getting cut at different lengths is also a property of the enzymes. Some enzymes leave a longer overhang, others a shorter one; some enzymes don't even leave an overhang. You will find more details in the video on restriction enzymes. Link: ua-cam.com/video/001BkPSM074/v-deo.html

  • @nartyalchin208
    @nartyalchin208 23 дні тому

    thank you very much for these videos

  • @raunitshrivastava6478
    @raunitshrivastava6478 23 дні тому

    Hats off for this video. It's extremely helpful for understanding. Thank you so much. I have a small doubt. Can you please explain the exact meaning of 'contagious' and 'non-contagious?

    • @theCrux
      @theCrux 23 дні тому

      When talking about ORFs, Contiguous = Continuous or Uninterrupted Non-Contiguous = Discontinuous or Interrupted For translation, ribosomes expect mature mRNA and therefore the ORF to be uninterrupted - which is true for bacteria at levels since they don't have introns; their ORF in mRNA = ORF in DNA. Eukaryotes (for intron-containing genes) have interrupted ORFs at DNA level and at the immature RNA level. Eventually, post-splicing, interruptions (introns) are removed to result in a continuous or contiguous ORF, ready for ribosomes.

    • @raunitshrivastava6478
      @raunitshrivastava6478 23 дні тому

      @@theCrux Got it. Thanks for explaining.

  • @theCrux
    @theCrux 23 дні тому

    Protein Expression Vector [Part 2] - ua-cam.com/video/x3xhV2ZZZpE/v-deo.html Protein Expression Vector [Part 3] - ua-cam.com/video/BhKzoIUH9_Q/v-deo.html Protein Expression Vector [Part 4]: ua-cam.com/video/JHRIA8v40qA/v-deo.html Protein Expression Vector [Part 5]: ua-cam.com/video/1PtIdAQoqME/v-deo.html

  • @ezzovonachalm9815
    @ezzovonachalm9815 24 дні тому

    The problem is : how long does the amount of injected mRNA continue producing SP ? An overproduction of SP ( the antigen) will exhaust the cells producing the antibody: and that is what we suspect in patients with "long covid": a massive reduction of antibody producing cells and consequently of the antibody = no cellular and no serologic immune deffences anymore- which would explain the explosion of turbocancers ! HOW to STOP mRNA's overproduction of the antigen which infiltrates tissues and creates deposits ( like in amyloidosis and other thesauropathies) Has the concentration of SP been measured and controlled in long covid patients ? I must suppose : not F de Clari MD 9.9.24

  • @Fox064
    @Fox064 26 днів тому

    Awesome videos. Finally someone going into detail. I hope this will help me to design my first plasmids. Thank you 🙏.

  • @sharathun
    @sharathun 27 днів тому

    i never leave youtube comments but your videos are too incredible to not thank you for making these. seriously some of the best details and explanations i have seen. would it be possible to request a video on the RNA interference pathway if you have a chance?

    • @theCrux
      @theCrux 26 днів тому

      Glad to hear that the content is useful :) And yes, RNAi will be part of this "Genetic Engineering" series. I hope to have that video up by the end of this year.

  • @Stillonelymp3
    @Stillonelymp3 27 днів тому

    your explanation is so excellent + illustrations makes it easy to understand!! thank you so much. do u perhaps cover topics in genetics? if so could we request a video on chromosome structure chemistry(detailed )+ linkages and recombination?

    • @theCrux
      @theCrux 27 днів тому

      Thanks for the suggestion. I do expect to cover Genetics and chromosome biology at some point. Currently caught up in videos on Genetic Engineering :)

    • @Stillonelymp3
      @Stillonelymp3 27 днів тому

      @@theCrux thank you so much. will be looking forward to it!

  • @Stillonelymp3
    @Stillonelymp3 27 днів тому

    saving lives!!! thank u so muchh

  • @nicolaslonchampt2531
    @nicolaslonchampt2531 28 днів тому

    These videos are really awesome and their quality and pedagogy are amazing. Would it be possible to have detailed video about how CRISPR Cas9 works and how it is used in genetic engineering?

    • @theCrux
      @theCrux 28 днів тому

      Yep, videos on CRISPR are in the works - to be published soon :)

    • @Fox064
      @Fox064 26 днів тому

      ​Do you plan to describe how is the whole editing system assembled for eukaryotic cells including vectors?

    • @theCrux
      @theCrux 26 днів тому

      Yes, I do plan to talk about vectors and editing system at a conceptual level... and maybe a short "How to design CRISPR vectors" video which I am still debating.

    • @nicolaslonchampt2531
      @nicolaslonchampt2531 26 днів тому

      @@theCrux oh yes, this would be a great topic to tackle!

    • @Fox064
      @Fox064 26 днів тому

      @@theCrux Thanks it would be great! Looking forward to see it.

  • @neuroknight
    @neuroknight 29 днів тому

    Thank you 😊 very much sir

  • @theCrux
    @theCrux 29 днів тому

    Protein Expression Vectors [Part 1]: ua-cam.com/video/3_WnvVgIkYo/v-deo.html

  • @jaasimahwani7466
    @jaasimahwani7466 Місяць тому

    can you please post a video on DNA topology and supercoiling

  • @EdT.-xt6yv
    @EdT.-xt6yv Місяць тому

    TY!

  • @zhifeiluo7536
    @zhifeiluo7536 Місяць тому

    Incredible explaination. Smart technology!

  • @AnjanaManigantan
    @AnjanaManigantan Місяць тому

    Wow u also collected information on positions of the genes Hats off Sir🥰😇

  • @sarbajitsengupta831
    @sarbajitsengupta831 Місяць тому

    The level of conceptual clarity you have is commendable. Keep it up.❤

  • @PsychologyNutshelled
    @PsychologyNutshelled Місяць тому

    6:09 the [ i ] also stands for inhibitor, as it inhibits the formation of the lacZYA gene

  • @PsychologyNutshelled
    @PsychologyNutshelled Місяць тому

    Damn! you ARE underrated

  • @curlygirl5925
    @curlygirl5925 2 місяці тому

    great content!

  • @simranmajeed5560
    @simranmajeed5560 2 місяці тому

    Thank you so much ❤

  • @vardhanpol821
    @vardhanpol821 2 місяці тому

    You need to post more vids bro, for all the helpless students over the world

  • @鈺雯黃-u6b
    @鈺雯黃-u6b 2 місяці тому

    Thanks for such an informative video! Your handwriting are so clear and well-organized, making me easily understand !

  • @sognatriceinarrestabile2457
    @sognatriceinarrestabile2457 3 місяці тому

    Finally... Everything makes sense!!! Thank you so much! You explained all the passages very thoroughly!

  • @anands1508
    @anands1508 3 місяці тому

    Sir, Am confused about the inner and outer strand .In many books, the inner strand is heavy(H) and outer (L).So could you please exaplain?

    • @theCrux
      @theCrux 3 місяці тому

      The way you draw inner and outer does not matter - there is no such thing as inner and outer as it means nothing. H and L carry meaning because it represents the base information. As long as you follow H and L, you are good! You can flip H and L to inner and outer (and vice verse) - just make sure to keep the direction of promoters consistent.

  • @bwalyansokolo432
    @bwalyansokolo432 3 місяці тому

    🎉🤓🙌🏾