NileRed! You don’t know how much your tutorial videos have helped me and TONS of other ochem students to visualize the labs. We need more lab technique videos, they’re so fun and educational to watch ❤
I'm doing a similar experiment for my chem lab course. This was very helpful in getting a sense of how exactly the set up will actually look. Thanks for sharing the video!
Hey man just came across your channel today and I freaking love it! Your experiments are so thorough, filmed very well and highly polished! Your channel makes me glad I chose to do chemistry at uni! Take care :D
@NileRed, hi at 3:17 you see the caffeine crystals in it's pure form. Isn't it toxic at this stage? In other words, isn't it dangerous to be near it without a mask? =(
Antony Liu; Well, yes and no. Pharmaceutical grade DCM wouldn't be an issue if COMPLETELY evaporated (this is MUCH harder to do than you might think. Even with a good vacuum, heating it for over a week and powderising the crystals every couple of days, the caffeine would still contain quite a bit of DCM that was incorporated into the crystal structue.). However, even a very pure AR grade of DCM, that you also fractionally distilled, MIGHT still contain some very toxic compounds (like carbon tetrachloride, mercury/lead salts, etc.), which would be VERY difficult to remove down to the required PPB level.
Dude this channel is amazing i have been looking for a chemistry channel to follow for quite some time now and your much easier to understand (orally) than NurdRage
NileRed also, where did you buy your equipment if you don't mind me asking, I recently became very interested in organic chemestry although I need to read many more books on the subject
Wow. You actually did a NMR analysis? that's pretty cool that someone like you was able to gain access to a NMR machine like that! Awesome video and keep up the good work!
Johnathan Griffin You never had pure caffeine? I have about 50 grams of it at home. It's not that bad but if you take too much (0,5 g at once is already too much), your heart rate goes up, you sweat and you'll get some really ugly headache while being nervous as hell. You need a 0,01g scale, measure out about 150 - 160 mg (equals about two coffees) and put it in whatever drink you want (it's bitter as fuck with a really unforgettable ugly taste), then you're good to go and start your day.
dopeofthepope .5 grams isn't that much for me, though it all depends on your tolerance. I60mg is about as much as your average 16oz energy drink, or 16oz of coffee. 200mg is a moderate dose for me, though I've done 1.1g with yohimbe HCl (a powerful natural stimulant) and that was fucking aweful. Though I know of someone who would do 3-5g per night while working overnights at walmart. Granted she started getting health problems. Though I agree completely on the taste that shit is TERRIBLE
I would like to share the method which was done out of curiosity " how is caffeine in colour"I took 5 grams of tea sample and to it I added 500 ml water and kept for boiling.on boiling I added 10 grams of heavy Magnesium Oxide to it and kept for boiling again for 2 hours. Adding water in same not exceeding the 500 ml volume just for the purpose not let the conical dry.then cooled the whole solution and filtered through 201 filter paper.Filtrate was 500 ml.I then took 200 ml filtrate and added 20 ml Sulphuric acid (2 ml sulphuric acid : 18 ml water).Took the whole solution in separating funnel and extracted 6 times with chloroform.combined all these layers of chloroform and kept on water-bath till whole chloroform evaporates.And here I get white coloured caffeine in good amount.Thank you!!
Have you tried extracting caffeine from soda pop with the same method? Basically just do a liquid-liquid extraction with a high-caffeine drink like Mountain Dew. I really like your videos. Keep up the good work.
I have done this experiment using Chloroform as it was easier for me to obtain. It worked great and my final product was white and very pure. I don't know which would remove more caffeine, Chloroform or Dichloromethane, but I am sure the difference is negligible.
@@ryanizadi3994 Yeah. Another odd thing about DCM (when I was researching it), was Wikipedia has it listed as a non-polar solvent, then on the solvent polarity page, its under polar solvents, lol.
So I followed your procedures to extract the caffeine from tea. I ran my caffeine through a 60MHz 1H NMR and I am seeing the normal caffeine peaks. However, I also see a peak around 1.75 ppm. Do you have any idea what this contamination may be?
Helio my friend, your videos are amazing. You are a very gifted chemist. I have a suggestion for a video for you and I don't know how to contact you so please excuse me here. There is a huge market for e-cigarette devices right now and regulations are now implemented in the US which classify all cigarette products is a tobacco product. my suggestion is that because e-cigarette juices and components will become very difficult to buy, maybe you could help with one of the biggest problems. The nicotine used in the E-Cigarette liquid is extracted from tobacco plants and comes in various strengths, the most popular being 100 milligrams per milliliter which is then further diluted with PG and vegetable glycerine. Would you be willing to do a video about how somebody could use tobacco leaves to extract the nicotine and have it dissolved in propylene glycol? Thanks again from a fellow Canadian.
+Austin Scriver I dont see why not. It would just start with the extraction using DCM though. But, I am not sure how many other products will come through with it.
If you had something dissolved in wax (carnuba wax specifically) that you needed to extract and convert to a water soluble salt, could you dissolve it from the wax using dcm/any nonpolar solvent then shake and vent like you did with an hcl solution? Brakleen didnt dissolve the wax but i am pretty sure it dissolved the something. It worked okay but i would like a professional's input for a more sound process.
You could evaporate it at 80°c (accelerates evaporation without exactly boiling) for a while, then once it reaches about a fifth of the volume it once had disable the heat , transfer it to a test tube or something that is small but tall and let it sit at an almost freezing temperature (the closest to freeze the best, but don't let it freeze though, use your fridge or put it in a batch of ice, but the ice is probably gonna melt off and the tube has to not move). You can then gently remove all the sludge above and the liquid below is diluted caffeine and caffeine crystals. You could put it at boiling temperature, shake it around and let it sit once more. Otherwise, you can replace DMC by boiling water, ethanol but NOT methanol (1mg/ml and toxic) but DMC is recommended because of caffeine's solubility being of (140 mg/ml) AND is non polar (easy to separate from the sludge). Water dissolves it in (22mg/ml). the hotter the water, the greater the solubility of the caffeine. However, ethanol is a much better solvent for caffeine, and should be able to dissolve much more at room temperature than water can. Provided that your ethanol is reasonably pure (as in no methanol, no butyl alcohol, ... basically, medicinal alcohol). *If you have the separatory funnel* , measure your volume of liquid and solid, and weight it. Now, add to a recipient of the exact same volume enough water until it's of that specific volume. Measure the weight difference between the teasludge and your water: Volume of tea sludge - Volume of water = 0L (since they're equal) Weight of tea sludge - Weight of water = Caffeine (& junk) weight Add VERY GENTLY TO THE BOTTOM OF YOUR FUNNEL WHERE THE CAFFEINE SHOULD SIT 0.045.. ml of ethanol for every mg of difference. It's slightly less than caffeine's STP solubility in water, which should do the trick with ethanol. Then wait a minute so it dissolves (do not stir, it'll mix the water and ethanol together since they're miscible with eachother); then let the clearish bottom seep out from your funnel. You've got your raw, slightly contaminated caffeine mix. Please let the alcohol evaporate in less than three days as caffeine ends up unstable in alcohol after three days . You can use your radiator to do that job, as it's temperature averages 40-50°c ( which makes alcohol more volatile). In case you have no heating system in your lab/home, just lay it on a/multiple watchglass-es/flat,non-sticky glassy surfaces. *Otherwise,* you can simply put your solution in it , let it sit for a while (about an hour or so) at an ice cold temperature to minimize the solubility of caffeine, then add gently to the bottom using an eyedropper/pipette/straw some ethanol. Here is a clean protocol using ethanol WITHOUT THE FUNNEL: 1. 1:40 Follow up to here, and if possible, up to 2:00 . 2. Let your sludge of caffeine and tea extract evaporate at 80°c in the beaker for a while, until your volume (for example, 200 ml) diminushes by four fifths (so that it's 40ml). Then let it cool to STP (room temp. and pressure) 3. Measure the volume of your oversaturated water-caffeine-tea-extract and it's weight. Weight will be refered to as "W" in my calculation and volume as "V" . I'll refer to it as "Liquid 1". 4. Prepare the same volume of water at STP that i'll refer to as "Liquid 1". (room temp. and press.) 5. Weight liquid 2. The difference between weight of Liquid 1 and Liquid 2 is your yield of caffeine (and other invasive particles.). Vliquid1-Vliquid2=0 (equality since same volume) Wliquid1-Wliquid2=Wcaffeine&etc To dilute your caffeine in ethanol, you'll pick it's solubility for caffeine which I wasn't able to find online , but knowing it's higher than Water's, we can deduce that it's above 22mg/ml and therefor, for every 22 miligrams of caffeine (&etc) you'll add add one mililitre of ethanol to your cold water solution to it's bottom (using a straw or a eyedropper/pipette) very gently. Then let it sit for one minute, and then, use said straw/eyedropper/pipette to pull the water out gently. You now have a ethanol/caffeine solution, hooray. Now boil off the ethanol at ~78°c until there's only the bottom left. What's left is a quite contaminated but pure enough caffeine end product. If you plan to consume it somehow which I don't recommend, you can wash it with REALLY COLD water since leftover alcohol and caffeine altogether won't do any good.
curious, how would you do a consumer test on caffeine supplements. Dissolve to get the solubles out, then try separate the caff from what ever else was soluble?
One question, Why did you make such a strong effort to conserve the water from boiling out. Correct me if im wrong, but caffeine does not evaporate so it should not be affected by the boiling process
When you repeated the process a second time at 1:30 , how much sodium carbonate did you add once again? 6 g? even if you only added 60 mL of water? Also do you have a written copy of your procedure? I am doing this experiment for my IB Chemistry EE, and will like to adapt your procedure for it.
@@8915s understandable, they're both very good solvents but also quite toxic or anesthetic. If you doesn't have a rotavapor, it's difficult to carry out the evaporation of the chloroform (assuming you don't wanna fall asleep ahahaha) Btw, some procedures used another (non tossic) solvent. Let me check.
Hi, first of all, I'm very grateful about your practical video of this extracting process. However, I have some questions that need to be answered about the concentration of each chemical compound that you've used in each step. Could you please reply my comment ? Thank you and have a great day
Very intresting video. How did you get rid off the rest of the organic meterial in there? Are they all acidic and got into the aquies layer at the begining when you added the base?
مرحبا صديقي محتواك رائع جدن تستحق التقدير هل ممكن استخلاص باقي القلويدات بهذه طريقه انا في منطقه صحراويه يكثر فيها قلويدات مفيده وقلويدات سامه اشكرك من قلبي ❤❤❤
Your using tea reminded me of a saying; a cook is a chemist whose results are edible. Would you consider presenting any gastronomically appealing experiments?
Are polyphenols the same as tannins? If not can someone provide an example of a tannin in black tea that is not a polyphenol? Also, what about pigments, do they stay behind in the aq layer? What about theobromine and l theanine and the other stuff in the plant? Like, what will happen to the l-theanine when dcm is added
+Maanuueel42 \m/(^_^) sorry i ment caffeine;and yes the amount of caffeine depends not on the tipe of tea but on different variables. you can find more on the internet
I did this experiment in organic chemistry and the caffeine I extracted had a yellowish tinge to it and had the smell of Mountain Dew. Was my caffeine pure?
soo if you would consume this,would it be toxic.i know caffeine is toxic in large doses but if you would consume the same amount that contains a cup of coffee
Great video, I am basing a research experiment for general chemistry off of it and the paper included. What is the molarity of the dichloromethane and sodium carbonate you are using? The paper does not specify either. I need to include molarity in my materials required for a proposal. I'm assuming it is just 1 M but a reply would be very helpful.
Hi! I'm writing a paper now also, would you like to share what molarity of the dichloromethane and sodium carbonate you used? I know it has been for a long time, and I would appreciate it if you could help me!
Nice video! So I read that the workup might be a little different if you used coffee instead of tea. Could you explain that to me? I'm a HS student that's just getting into all this all too cool stuff... :) Again nice work!
Not really. You need the solvent to be immiscible with water. So you're really only left with diethyl ether, chloroform, ethyl acetate, and some others. Of those, DCM and chloroform work the best by far.
Would another solvent work like ethanol or isopropanol if an ionic compound like sodium chloride was added first to the water/tea solution first? Many Thanks!
Daily Insanity You really need to use a solvent that is not miscible with water and that can easily dissolve caffeine. DCM is a very good fit for both of these requirements. However, even with a lot of salt, I doubt the other solvents will work very well
Dissolve it in small amounts of chloroform, wash it a few times with chloroform and transfer to the test time and then evaporate. You could also just scrape off the caffeine with a spatula and then transfer it dry, but some would be left behind.
***** A can of Monster would have about the same amount of caffeine but would also contain many other chemicals that are soluble in DCM which could be hard to separate later.
+Cave Johnson(XP) You might also be able to try coffee beans, however I'm not sure if there would be much of a difference in the amount of caffeine extracted.
I very much doubt that any soft drink contains as much caffeine as that tea concentrate. Tea is the most concentrated readily available natural souce of caffeine, containing about twice as much as coffee, gram by gram. A cup of coffee contains much more caffeine that a cup of tea, but that's just because using the same weight of tea per cup as we do for coffee would make an undrinkably astringent and bitter brew.
Yes, it definitely is. There are a few procedures that call for NaOH instead of the carbonate. I am not sure how much to use, but it is definitely a low concentration.
I would like to do this as a demonstration but I don't have access to Cl2CH2. Does anyone know of a suitable substitute available from hardware stores or similar?
Hey I'm doing my Chemistry project on same topic but I doubt that our school lab has neither dichloromethane nor chloroform in stock can you please answer Can any other organic solvent be used for this caffeine extraction or some other chemicals for same results except DCM or Chloroform!?
You can use a soxhlet, but I found a link that suggests that the solubility of caffeine in ethyl acetate is very close to water. Whereas the solubility in DCM or chloroform is >9x more.
You can use ethyl acetate, but the yield will suffer because more will be left in the water layer. You might be able to fix that by increasing the number of washings. You could also evaporate all the water and dissolve the caffeine from the remnants using methanol or something.
NileRed! You don’t know how much your tutorial videos have helped me and TONS of other ochem students to visualize the labs. We need more lab technique videos, they’re so fun and educational to watch ❤
I'm doing a similar experiment for my chem lab course. This was very helpful in getting a sense of how exactly the set up will actually look. Thanks for sharing the video!
Thank you for including an NMR! Nice to see more chem methods to prove a sample.
Aa
Hey man just came across your channel today and I freaking love it! Your experiments are so thorough, filmed very well and highly polished! Your channel makes me glad I chose to do chemistry at uni! Take care :D
jake n Thanks, I am glad you like them!
@NileRed, hi at 3:17 you see the caffeine crystals in it's pure form. Isn't it toxic at this stage? In other words, isn't it dangerous to be near it without a mask? =(
Not that toxic. We are talking about 30mg solid. It's not going in your body.
What if you wanted to extract green tea caffeine for personal ingestion. Would the DCM become a problem even if it's been fully evaporated?
Antony Liu; Well, yes and no.
Pharmaceutical grade DCM wouldn't be an issue if COMPLETELY evaporated (this is MUCH harder to do than you might think. Even with a good vacuum, heating it for over a week and powderising the crystals every couple of days, the caffeine would still contain quite a bit of DCM that was incorporated into the crystal structue.).
However, even a very pure AR grade of DCM, that you also fractionally distilled, MIGHT still contain some very toxic compounds (like carbon tetrachloride, mercury/lead salts, etc.), which would be VERY difficult to remove down to the required PPB level.
Dude this channel is amazing i have been looking for a chemistry channel to follow for quite some time now and your much easier to understand (orally) than NurdRage
Thanks!
NileRed how much do you think it would cost, overall, if I wanted to repeat this experiment at home? equipment and everything.
NileRed also, where did you buy your equipment if you don't mind me asking, I recently became very interested in organic chemestry although I need to read many more books on the subject
no wonder chemists get the best coffee
haha
Wow. You actually did a NMR analysis? that's pretty cool that someone like you was able to gain access to a NMR machine like that! Awesome video and keep up the good work!
+Classical Liberal what do you mean 'someone like him' lol; he's a chemist, who else would have one
He meant that he is an amature.
prolly not an amateur
My OChem professor is awesome and assigned us this video to prepare for a similar lab, love your videos.
Yeah, pure Caffeine instead of Coffee in the morning :-)
too bad it most likely kill you if you took too much. It's easy to die of caffeine overdose, just 37 bottles of redline would kill you :P (3oz each)
Johnathan Griffin Ld50 of pure caffeine is like 8 - 10 grams, so no dying is quite unlikely
Johnathan Griffin You never had pure caffeine? I have about 50 grams of it at home. It's not that bad but if you take too much (0,5 g at once is already too much), your heart rate goes up, you sweat and you'll get some really ugly headache while being nervous as hell. You need a 0,01g scale, measure out about 150 - 160 mg (equals about two coffees) and put it in whatever drink you want (it's bitter as fuck with a really unforgettable ugly taste), then you're good to go and start your day.
dopeofthepope
.5 grams isn't that much for me, though it all depends on your tolerance. I60mg is about as much as your average 16oz energy drink, or 16oz of coffee. 200mg is a moderate dose for me, though I've done 1.1g with yohimbe HCl (a powerful natural stimulant) and that was fucking aweful. Though I know of someone who would do 3-5g per night while working overnights at walmart. Granted she started getting health problems.
Though I agree completely on the taste that shit is TERRIBLE
+dopeofthepope Why not just drink a cup of coffee if you're going through that much effort?
Neat, I will update my morning coffee routine
I would like to share the method which was done out of curiosity " how is caffeine in colour"I took 5 grams of tea sample and to it I added 500 ml water and kept for boiling.on boiling I added 10 grams of heavy Magnesium Oxide to it and kept for boiling again for 2 hours. Adding water in same not exceeding the 500 ml volume just for the purpose not let the conical dry.then cooled the whole solution and filtered through 201 filter paper.Filtrate was 500 ml.I then took 200 ml filtrate and added 20 ml Sulphuric acid (2 ml sulphuric acid : 18 ml water).Took the whole solution in separating funnel and extracted 6 times with chloroform.combined all these layers of chloroform and kept on water-bath till whole chloroform evaporates.And here I get white coloured caffeine in good amount.Thank you!!
Awesome iv been waiting for this one for a while !!! Always looking forward to more!!
Have you tried extracting caffeine from soda pop with the same method? Basically just do a liquid-liquid extraction with a high-caffeine drink like Mountain Dew.
I really like your videos. Keep up the good work.
I have done this experiment using Chloroform as it was easier for me to obtain. It worked great and my final product was white and very pure. I don't know which would remove more caffeine, Chloroform or Dichloromethane, but I am sure the difference is negligible.
I think they should both work equally well!
Nice work! You should do more caffeine related work!
Thanks for the video, i made it at school, but isn't so clean and pure.
2:17 so the caffeine is in the DCM layer? DCM/Methylene Chloride isnt listed as a solvent on the caffeine Pubchem page. Hmm
Thats odd, a quick google search would disprove pubchem
@@ryanizadi3994 Yeah. Another odd thing about DCM (when I was researching it), was Wikipedia has it listed as a non-polar solvent, then on the solvent polarity page, its under polar solvents, lol.
Lmfao, controversy over a known substance, thats the dumbest shit I’ve seen in 2020
So I followed your procedures to extract the caffeine from tea. I ran my caffeine through a 60MHz 1H NMR and I am seeing the normal caffeine peaks. However, I also see a peak around 1.75 ppm. Do you have any idea what this contamination may be?
tanines, and some organic junk i suggest
Love your videos man,
Hypothetically this is exactly how you extract DMT * Joe Rogan wants to know your location*
No. That's generally acid base extraction if I remember correctly.
Guess what am I extracting from tea today....Thanks man, it was a huge help.
If done outside of a laboratory with clean food grade equipment, would the yield from this process be considered suitable for consumption?
Helio my friend, your videos are amazing. You are a very gifted chemist. I have a suggestion for a video for you and I don't know how to contact you so please excuse me here. There is a huge market for e-cigarette devices right now and regulations are now implemented in the US which classify all cigarette products is a tobacco product. my suggestion is that because e-cigarette juices and components will become very difficult to buy, maybe you could help with one of the biggest problems. The nicotine used in the E-Cigarette liquid is extracted from tobacco plants and comes in various strengths, the most popular being 100 milligrams per milliliter which is then further diluted with PG and vegetable glycerine. Would you be willing to do a video about how somebody could use tobacco leaves to extract the nicotine and have it dissolved in propylene glycol? Thanks again from a fellow Canadian.
Michael Wolfe nicotine is really toxic and illegal to have :/
@@jakublewandowski318 also 100 mg is like ez leathal, 10mg is more realistic
Still a dangerous, addictive drug
Could you please give a hint how to extract tannin with household chemicals? Thanks a lot!
Could the same process be done for something like an energy drink?
+Austin Scriver I dont see why not. It would just start with the extraction using DCM though. But, I am not sure how many other products will come through with it.
Wow.. You predicted the future
If you had something dissolved in wax (carnuba wax specifically) that you needed to extract and convert to a water soluble salt, could you dissolve it from the wax using dcm/any nonpolar solvent then shake and vent like you did with an hcl solution? Brakleen didnt dissolve the wax but i am pretty sure it dissolved the something. It worked okay but i would like a professional's input for a more sound process.
Nice, but can u use something else than DMC? If DMC isnt available, could u use something else?
You could evaporate it at 80°c (accelerates evaporation without exactly boiling) for a while, then once it reaches about a fifth of the volume it once had disable the heat , transfer it to a test tube or something that is small but tall and let it sit at an almost freezing temperature (the closest to freeze the best, but don't let it freeze though, use your fridge or put it in a batch of ice, but the ice is probably gonna melt off and the tube has to not move).
You can then gently remove all the sludge above and the liquid below is diluted caffeine and caffeine crystals. You could put it at boiling temperature, shake it around and let it sit once more.
Otherwise, you can replace DMC by boiling water, ethanol but NOT methanol (1mg/ml and toxic) but DMC is recommended because of caffeine's solubility being of (140 mg/ml) AND is non polar (easy to separate from the sludge).
Water dissolves it in (22mg/ml).
the hotter the water, the greater the solubility of the caffeine.
However, ethanol is a much better solvent for caffeine, and should be able to dissolve much more at room temperature than water can. Provided that your ethanol is reasonably pure (as in no methanol, no butyl alcohol, ... basically, medicinal alcohol).
*If you have the separatory funnel* , measure your volume of liquid and solid, and weight it.
Now, add to a recipient of the exact same volume enough water until it's of that specific volume. Measure the weight difference between the teasludge and your water:
Volume of tea sludge - Volume of water = 0L (since they're equal)
Weight of tea sludge - Weight of water = Caffeine (& junk) weight
Add VERY GENTLY TO THE BOTTOM OF YOUR FUNNEL WHERE THE CAFFEINE SHOULD SIT 0.045.. ml of ethanol for every mg of difference.
It's slightly less than caffeine's STP solubility in water, which should do the trick with ethanol. Then wait a minute so it dissolves (do not stir, it'll mix the water and ethanol together since they're miscible with eachother); then let the clearish bottom seep out from your funnel. You've got your raw, slightly contaminated caffeine mix. Please let the alcohol evaporate in less than three days as caffeine ends up unstable in alcohol after three days . You can use your radiator to do that job, as it's temperature averages 40-50°c ( which makes alcohol more volatile). In case you have no heating system in your lab/home, just lay it on a/multiple watchglass-es/flat,non-sticky glassy surfaces.
*Otherwise,*
you can simply put your solution in it , let it sit for a while (about an hour or so) at an ice cold temperature to minimize the solubility of caffeine, then add gently to the bottom using an eyedropper/pipette/straw some ethanol.
Here is a clean protocol using ethanol WITHOUT THE FUNNEL:
1. 1:40 Follow up to here, and if possible, up to 2:00 .
2. Let your sludge of caffeine and tea extract evaporate at 80°c in the beaker for a while, until your volume (for example, 200 ml) diminushes by four fifths (so that it's 40ml). Then let it cool to STP (room temp. and pressure)
3. Measure the volume of your oversaturated water-caffeine-tea-extract and it's weight. Weight will be refered to as "W" in my calculation and volume as "V" . I'll refer to it as "Liquid 1".
4. Prepare the same volume of water at STP that i'll refer to as "Liquid 1". (room temp. and press.)
5. Weight liquid 2. The difference between weight of Liquid 1 and Liquid 2 is your yield of caffeine (and other invasive particles.).
Vliquid1-Vliquid2=0 (equality since same volume)
Wliquid1-Wliquid2=Wcaffeine&etc
To dilute your caffeine in ethanol, you'll pick it's solubility for caffeine which I wasn't able to find online , but knowing it's higher than Water's, we can deduce that it's above 22mg/ml and therefor, for every 22 miligrams of caffeine (&etc) you'll add add one mililitre of ethanol to your cold water solution to it's bottom (using a straw or a eyedropper/pipette) very gently. Then let it sit for one minute, and then, use said straw/eyedropper/pipette to pull the water out gently.
You now have a ethanol/caffeine solution, hooray.
Now boil off the ethanol at ~78°c until there's only the bottom left.
What's left is a quite contaminated but pure enough caffeine end product.
If you plan to consume it somehow which I don't recommend, you can wash it with REALLY COLD water since leftover alcohol and caffeine altogether won't do any good.
I really like watching your videos.
curious, how would you do a consumer test on caffeine supplements. Dissolve to get the solubles out, then try separate the caff from what ever else was soluble?
I'd like to see a broad spectrum black tea polyphenol extract, keeping the caffeine within the black tea extract.
in the absence of sodium carbonate, could it be substituted with sodium bicarbonate?
what about sodium hydroxide? would that work?
One question, Why did you make such a strong effort to conserve the water from boiling out. Correct me if im wrong, but caffeine does not evaporate so it should not be affected by the boiling process
can you show me: The effect of the time of boiling (1min, 2mins, 3mins, 4mins, 5mins) on the amount of caffeine found in 3 bags of green tea.
When you repeated the process a second time at 1:30 , how much sodium carbonate did you add once again? 6 g? even if you only added 60 mL of water? Also do you have a written copy of your procedure? I am doing this experiment for my IB Chemistry EE, and will like to adapt your procedure for it.
wait are you allowed to use DCM or chloroform in school? I want to do this experiment as well lmao pls send help
@@8915s we're doing it in my high school, in italy. Using chloroform, because we don't have any DCM.
Oh :( neither are allowed at my school lol
@@8915s understandable, they're both very good solvents but also quite toxic or anesthetic. If you doesn't have a rotavapor, it's difficult to carry out the evaporation of the chloroform (assuming you don't wanna fall asleep ahahaha)
Btw, some procedures used another (non tossic) solvent. Let me check.
Ethilic acetate, still not as productive as chloroform but definitely not toxic
Hi, first of all, I'm very grateful about your practical video of this extracting process. However, I have some questions that need to be answered about the concentration of each chemical compound that you've used in each step. Could you please reply my comment ? Thank you and have a great day
This video is 8 years old and the demonstrator now has 5 million subscribers, I'm sorry I don't think he will answer
I have a question, how do you determine the mass of sodium carbonate to put in the caffeine solution ?
Very intresting video. How did you get rid off the rest of the organic meterial in there? Are they all acidic and got into the aquies layer at the begining when you added the base?
مرحبا صديقي محتواك رائع جدن تستحق التقدير هل ممكن استخلاص باقي القلويدات بهذه طريقه انا في منطقه صحراويه يكثر فيها قلويدات مفيده وقلويدات سامه اشكرك من قلبي ❤❤❤
Isn't it better to brew the tea and remove the bags before you add the sodium carbonate? Is freebase caffeine water soluble?
Can I use xylene instead of DCM ? =) thanks
Great video!
And..some question
Why caffeine transfer water to DCM?
caffeine DCM solulibility is higher than water?
Yes, the solubility in DCM is much higher, almost 9x higher if i recall correctly.
Can we heat the solution directly without using downward distillation???
Hi! How could we extract tannin alone? Thank you.
Your using tea reminded me of a saying; a cook is a chemist whose results are edible. Would you consider presenting any gastronomically appealing experiments?
Are polyphenols the same as tannins? If not can someone provide an example of a tannin in black tea that is not a polyphenol? Also, what about pigments, do they stay behind in the aq layer? What about theobromine and l theanine and the other stuff in the plant? Like, what will happen to the l-theanine when dcm is added
stupid question but was the bottom layer the organic layer?
Nice vid man
you said green tea? Green tea is very low in caffiene, try black tea next time, it contains over 5 times more
it's just a mith
there's the same amount of nicotine
+MaPorcaTrotaATutti
lol?
***** well, he's not wrong. There is no nicotine in either, so there indeed is the same amount of nicotine in green and black tea xD
+Maanuueel42 \m/(^_^) sorry i ment caffeine;and yes the amount of caffeine depends not on the tipe of tea but on different variables.
you can find more on the internet
MaPorcaTrotaATutti ^^
Can we measure the amount of Tannins? as they interact with sodium carbonate
What happend about theobromine and theine extracted from tea leaves? There is no possibility to find these components in DCM layer after extraction?
I did this experiment in organic chemistry and the caffeine I extracted had a yellowish tinge to it and had the smell of Mountain Dew. Was my caffeine pure?
No pure caffeine is white. Some impurities it seems. From my knowledge it must be white that is.
Try using a soxhlet extractor for the first part. Should work much better
soo if you would consume this,would it be toxic.i know caffeine is toxic in large doses but if you would consume the same amount that contains a cup of coffee
Would adding NaCO3 after brewing the tea save you some NaCO3 potentially?
Great video, I am basing a research experiment for general chemistry off of it and the paper included. What is the molarity of the dichloromethane and sodium carbonate you are using? The paper does not specify either. I need to include molarity in my materials required for a proposal. I'm assuming it is just 1 M but a reply would be very helpful.
Hi! I'm writing a paper now also, would you like to share what molarity of the dichloromethane and sodium carbonate you used? I know it has been for a long time, and I would appreciate it if you could help me!
@@alicetsang6274 lmk if you get an answer anywhere!
Is there a chemical I could use instead of dichloromethane? As it is really toxic I am trying to find a chemical to replace it with.
Hey ... nice illustration...can you please tell me that what the 5% impurities in 95% pure caffeine??
Can I use this method with Chinese tea (Ma-Hoang sinica?) Any Tricks or tips? For science only. Thank you, Love your channel.
Can you use magnesium sulfate instead of calcium chloride
What would the NMR look like if it wasnt pure?
Nice video! So I read that the workup might be a little different if you used coffee instead of tea. Could you explain that to me? I'm a HS student that's just getting into all this all too cool stuff... :) Again nice work!
How did it go from that orange/yellow color to a clear white color at 2:23 to 2:27?
Honestly, that does look a little weird. However, it is because I washed it with saturated salt solution. It cleaned it up a little.
Nile Red can i use anything more easily available instead of dichloromethane.
Not really. You need the solvent to be immiscible with water. So you're really only left with diethyl ether, chloroform, ethyl acetate, and some others. Of those, DCM and chloroform work the best by far.
Erm question. Where the hell do you get the parts you used in this video.
How can you do this without a separatory funnel?
thanks for the great video
Coud you use "Essigsäure ethylester " instead of dcm?
i would like to know if can i apply the same procedure for the extraction of caffeine from Energy drink ( 5 hours energy drink)?
What nmr machine would you recommend for a table top machine
Strong passive voice in chemistry
Is it possible to be done without sodium carbonate? CAN YALL RESPOND NOW x
COULD YOU EXPLAIN THE PROCESS TO EXTRACT TANNIN FROM TEA LEAVES
how can calcium chloride can dried the organic layer?
Can I use chloroform instead of DCM and also get good results ?
may i ask about the chemical equation of sodium carbonate and tannins in tea
Would another solvent work like ethanol or isopropanol if an ionic compound like sodium chloride was added first to the water/tea solution first? Many Thanks!
Daily Insanity You really need to use a solvent that is not miscible with water and that can easily dissolve caffeine. DCM is a very good fit for both of these requirements. However, even with a lot of salt, I doubt the other solvents will work very well
Thanks ❤
How about extracting iodine from kelp? That would be super cool.
Is there any way at all I can contact you? your experiments are pretty cool.
What is the function of adding Na2CO3?
Could you do this via soxhlet extraction?
Nice and simple. I can use this for DMT, right?
how would percentage yield be calculated in this experiment
How do you remove the dry, crude caffeine from the inside of the round bottom flask?
Dissolve it in small amounts of chloroform, wash it a few times with chloroform and transfer to the test time and then evaporate.
You could also just scrape off the caffeine with a spatula and then transfer it dry, but some would be left behind.
How do you access to NMR?!
Can we replace Na2CO3 with CaCO3?
Can you make high purity kratom extract?
What can i use as a substitute for dichloromethane?
Could you use something like monster instead of tea? To hopefully attain more caffiene?
***** A can of Monster would have about the same amount of caffeine but would also contain many other chemicals that are soluble in DCM which could be hard to separate later.
NotSoLogical Thanks for the info! So Tea would actually be more advantageous?
Yep
+Cave Johnson(XP) You might also be able to try coffee beans, however I'm not sure if there would be much of a difference in the amount of caffeine extracted.
I very much doubt that any soft drink contains as much caffeine as that tea concentrate. Tea is the most concentrated readily available natural souce of caffeine, containing about twice as much as coffee, gram by gram. A cup of coffee contains much more caffeine that a cup of tea, but that's just because using the same weight of tea per cup as we do for coffee would make an undrinkably astringent and bitter brew.
Is sodium hydroxide a suitable base as a substitution for using sodium carbonate for neutralizing the tannins?
Yes, it definitely is. There are a few procedures that call for NaOH instead of the carbonate. I am not sure how much to use, but it is definitely a low concentration.
I would like to do this as a demonstration but I don't have access to Cl2CH2. Does anyone know of a suitable substitute available from hardware stores or similar?
nice work
nice video, i just fell in love with your channel at first sight, i was wonder would this method work to extract thc from cannabis
Jorge Gonzalez no
no but i think this is how you extract ephedrine from ma huang which can then be turned into amphetamine and methamphetamine
So 6 teabags of green tea is only 30mg?
What does pure caffeine taste like?
Why did not use coffee for extrating coffein?
Cihangir DURSUN I guess I could have, I just thought the tea in the tea bags was going to be less messy.
Hey I'm doing my Chemistry project on same topic but I doubt that our school lab has neither dichloromethane nor chloroform in stock can you please answer
Can any other organic solvent be used for this caffeine extraction or some other chemicals for same results except DCM or Chloroform!?
Is this meant for using or is it toxic or something
Could I try the hole process with water in a soxhlet extractor which i could then extract witch ethyl acetate??
You can use a soxhlet, but I found a link that suggests that the solubility of caffeine in ethyl acetate is very close to water. Whereas the solubility in DCM or chloroform is >9x more.
Nile Red I don't have DCM or chloroform available. Which else solvents could I use??
You can use ethyl acetate, but the yield will suffer because more will be left in the water layer. You might be able to fix that by increasing the number of washings. You could also evaporate all the water and dissolve the caffeine from the remnants using methanol or something.
could you use the same this way with coffee?
You dont work under fume hood with DCM ? 😮