Minor correction: Eukaryotic RNA polymerases have ~12 subunits (in the video at around 11:20 I wrongfully said that it is ~26). I confused 26 from the fact that it is the number of YSPTSPS repeats in yeast CTD (humans have 52 repeats, yeast have 26). Additional fact: Plants also have RNA polymerase V that is involved in a similar non-essential function as RNA polymerase IV. To read more about them, check out www.ncbi.nlm.nih.gov/pmc/articles/PMC4618083/
The best explanation there is on UA-cam for transcription ....... I've searched for a video like this for quite a time now and i finally found It.....Thank you soo much
Omg......can't tell in words....finally it was a great great video.. Thanks a lott and expecting more such videos...I have watched other channels...but this was the best. thank you very much
Hi, thanks for the super-useful video! A small doubt: Why rRNA is most abundant in the cell compared to mRNA and tRNA? Can we tell because most mRNA gets converted to proteins and is unstable when not bound to ribosomes. But rRNA are stable as they are bound to ribosomes?
Ribosomes are in excess of mRNAs. mRNAs tend to be reused to make proteins - same mRNA can be used for translation for as long as it is around in the cytoplasm, so you don't have a lot of them to begin with. Typically you have around 100k - 300k mRNAs per cell vs millions of rRNA because you have way too many ribosomes. The excess of ribosomes means translation is immediate.
Thank u for right to the point and very informative sharing this save many students time n future better than my professor lecture! ☝️🙏🎃🎃🎃Happy Halloween!
RNA polymerase moves from 3' of the template to the 5' - the resulting RNA has 5' end coming out first (so the RNA is 5' > 3'). The chemistry of synthesis is such that you can only synthesize nucleic acids in 5' > 3' direction. The enzyme on the other hand may move in either direction. Check out 'Strand Notations' at 2:52 in this video ua-cam.com/video/7kfqeIjIzMY/v-deo.html&ab_channel=theCrux
Is it really true though that prokaryotic dna is not associated with proteins? That's a historical misconception isn't it? Just 2 months ago I learned about H-NS. (Not trying to be a smart-ass. Love your channel
True, prokaryotic DNA is not naked DNA. It does have a lot of proteins associated with it - just not the same level of compaction provided by associated structural organizers we see in eukaryotes. H-NS, NAPs, HUs, FIS and many more are great examples of prokaryotic DNA organizers. I should have been more careful with my choice of words (at the cost of over-simplification) in this video. Thanks for pointing it out!
Minor correction: Eukaryotic RNA polymerases have ~12 subunits (in the video at around 11:20 I wrongfully said that it is ~26). I confused 26 from the fact that it is the number of YSPTSPS repeats in yeast CTD (humans have 52 repeats, yeast have 26).
Additional fact: Plants also have RNA polymerase V that is involved in a similar non-essential function as RNA polymerase IV. To read more about them, check out www.ncbi.nlm.nih.gov/pmc/articles/PMC4618083/
Lewins ✌️
The best explanation there is on UA-cam for transcription ....... I've searched for a video like this for quite a time now and i finally found It.....Thank you soo much
Glad it was helpful! :)
Watching your channel makes me secrete serotonin !!! Thank you ...
I am glad it is helpful and enriching your education :)
this was really helpful , with all the information and notes. Thank you !!
excellent explanation all details in a single slide
I love the animation, so clean and neat! The video is def underrated, learnt alot, thank you!
very helpful and informative. I really need this video a lot.
Your content is best... I learnt my entire semester from your videos..... Great job...
I shared this video to my class group 😇😇
Thanks a lot 😊 It brings me great joy to hear that the content has been useful.
thxu so much, really help!
Omg......can't tell in words....finally it was a great great video.. Thanks a lott and expecting more such videos...I have watched other channels...but this was the best. thank you very much
The best explanation!! Thanks
Glad it was helpful!
ossum explanations
Keep it up, great work ✌👌👏👍
Thanks a lot 😊
Nice explanation.
Glad it was helpful! :)
amazing thank you 🌷🌷🌷🌷
Thanku
It must be that mRNA produced must be in 3 to 5 direction, complementary to DNA template strand
Hi, thanks for the super-useful video!
A small doubt: Why rRNA is most abundant in the cell compared to mRNA and tRNA? Can we tell because most mRNA gets converted to proteins and is unstable when not bound to ribosomes. But rRNA are stable as they are bound to ribosomes?
Ribosomes are in excess of mRNAs. mRNAs tend to be reused to make proteins - same mRNA can be used for translation for as long as it is around in the cytoplasm, so you don't have a lot of them to begin with. Typically you have around 100k - 300k mRNAs per cell vs millions of rRNA because you have way too many ribosomes. The excess of ribosomes means translation is immediate.
@@theCrux Oh... understood.
Thanks for clarifying!
Thank u for right to the point and very informative sharing this save many students time n future better than my professor lecture! ☝️🙏🎃🎃🎃Happy Halloween!
Glad it was helpful!
Sir,I have a question : the RNA polymerase works in 5 to 3 direction of DNA template, then y does it produce mRNA in the 5 to 3direction???
RNA polymerase moves from 3' of the template to the 5' - the resulting RNA has 5' end coming out first (so the RNA is 5' > 3'). The chemistry of synthesis is such that you can only synthesize nucleic acids in 5' > 3' direction. The enzyme on the other hand may move in either direction. Check out 'Strand Notations' at 2:52 in this video ua-cam.com/video/7kfqeIjIzMY/v-deo.html&ab_channel=theCrux
Is it really true though that prokaryotic dna is not associated with proteins? That's a historical misconception isn't it?
Just 2 months ago I learned about H-NS.
(Not trying to be a smart-ass. Love your channel
True, prokaryotic DNA is not naked DNA. It does have a lot of proteins associated with it - just not the same level of compaction provided by associated structural organizers we see in eukaryotes. H-NS, NAPs, HUs, FIS and many more are great examples of prokaryotic DNA organizers. I should have been more careful with my choice of words (at the cost of over-simplification) in this video. Thanks for pointing it out!
You know, if only that scientist named the transcription factors alphabetically instead of that weird arbitrary rules this wouldn't be that hard lol
Your hand writting are verry small to read. Make it bigger
Thanks for the feedback. I will try to make it bigger.