5 Best TLC Staining Agents That Will Help You In Chemistry Lab Every Time! | Dr. Aman

If your SM or your product is not UV active then you can’t see them under UV chamber and hence you can’t use this technique. Now to deal with this situation the staining agents comes into picture but before learing about staining agents. To be UV-active, compounds must possess a certain degree of conjugation, which occurs most commonly in aromatic compounds if your compound doesn’t have certain level of double bond conjugation then they are UV inactive.
Fortunately, there are a number of permanent or semi-permanent methods for visualization which will not only allow one to see these compounds but also provide a method for determining what functional groups are contained within the molecule. This method is referred to as staining the TLC plate, and experience will allow you to determine what functional groups will appear as what color upon visualization.
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[@tejaspatel9837]

continue your journey....... best of luck.......good works

[@sreevidhya9550]

You nailed it sir

[@jyotiprabhamishra2152]

Thank you

[@msmeenu2125]

Hi, the content was highly informative 👏🏻.. can you suggest a stain for acetamide specifically?

[@BeaconBinScience]

Ninhydrin can be used.It gives reddish brown churring while with amine purple churring is confirmed.
You can also use cerium molybdate solution,it is also an option

[@msmeenu2125]

​@@BeaconBinScienceI did with ninhydrin but acetamide spot is not visible.. will PMA help in this case?

[@BeaconBinScience]

@@msmeenu2125 you can check that with PMA, but I am not sure.

[@nasrin_05]

👌👌👌❤

[@janvisharma2048]

😁🔥🔥