You are the sole individual responsible for me wanting to continue science and I mean it! Since I came across your videos back in 2019 it has changed my perspective entirely! I’ve grown fond of my subjects due to clarity of concepts and my grades improved massively straight from a 9.00 GPA to 9.88. All thanks to this man right here!
@@taladiv3415 Haha I’ll take that as a compliment, so there were so many students scoring similar or better that it wasn’t a deal at all, after the jump I could atleast make it to some rank if not the 1st.
Dear sir, I used to watch your videos during my MD residency (Biochemistry). (2014 -17) and since I am watching your all videos regularly. Your technique for teaching and your fundamentals direct set and hit in mind. after so long I want to say thank you. THANK YOU. Edited: I bookmarked your few videos as a important one for examination as well as for reference.
@@shomusbiologyofficial So the virus (phage) can never kill bacteria if they have CRISP mechanism on them, right? What happens if the adaptive immunity has no S sequence already present for the new viral DNA injected inside the bacteria/microbes?
Sir i am a veterinary student, i done my biotechnology portion under veterinary microbiology fully from your videos. You are such a fab teacher and i am glad to be your student through this virtual youtube mode.
Instant subscribe, I've watched a bunch of videos on cripsr before this but no one started with basics and the way u explained is god teir 🛐 Thank you so much sensei
U literally saved me sir!.... I m struggling with this topic since last 2 days & after watching ur video the topic is very clear to me... Thank you so much 😇
Tu contenido es increíble, tanto que una profesora de mi Universidad usa tus videos como referencias para aclararnos temas, sigue así. 😊😊😊😊😊👏🏻👏🏻👏🏻👏🏻👏🏻👏🏻
Dear Shomu sir, Just want to let you know that your lectures are loved by everyone who's into a field related to biotech. I am a student from IIT Kanpur and I always watch ur videos before my exams. Thank you!
Sir , I am is class 12th I am reading my biotechnology chapter and same time I get information about the Nobel prize given and started to get information about it . I got your video and it cleared my all doubt and now I am going to make project with using your applications of CRISPER discussed at last to present my school in online Science exhibition
always sir.... getting the benefit since i was doing bsc now i am in my msc 3rd semester and preparing for csir and your vedios are helpfull in many ways thank you
@@shomusbiologyofficial sir ek question that nei abhi Bsc 2nd year mei hu Muje padhnee mei mazaa toh ata h Lekin Muje ye nhi pta ki future mei mai kya krunga koi solution h toh bata dijiye.
you are doing a Commendable job. Super! You've got your brain in gear today. We all are proud of you and proud of your efforts. I just want to request you that stay safe and take care.
@@SidraLively hi i am intrested to work in cancer biology crispr editing ti target cancer cell this is my rough idea Plz suggest me something better if or help me to prepare a proposal
Thank you so much for your videos. Your explanations are always very helpful and allow to understand even difficult processes. Thanks to your visualizations I can finally remember everything. You're an excellent teacher!
This is a Great Gift For Human Kind.. The Scientists Who Invented a Sharp tool,Of Drafting of DNA , Immanuel Charpientier, And Jennifer Doudna Are Next Level Angels in Medicine field.
Sir I'm watching your videos since class 11. I was really confused about my future education after 12th. You're the one Sir , who inspired me to take Biotechnology as my subject after 12th. In the month of June , 2021 , My HS will be over. And after that I can pursue my dream Subject , only because of you Sir❤️. Thank You So much Sir providing us these Amazing conceptual videos on almost every aspects of Modern Biology. Thank You again ❤️🔥
I was more like a " studying just to pass" student....then I came across your videos..I've topped my college in masters and now I've qualified my GATE. I owe you my confidence in my subject 😌 I somehow have this urge to make you proud 😂
I’m in class 12 and I’m really interested in gentics tho it’s out of my syllabus. I found this vedio and it’s really interesting.Thanks sir for giving this very useful lecture. I have understand everything about CRISPR- Cas9🙌🏻❤️
Thank you very much sir for such a very valuable guidance, making it easily understandable, i found it very helpful for my seminar today,i got praised by my lecturers 😇
Your videos are very helpful. I have a doubt. If the CRISPR is an RNA template, then how to binds to DNA having complementary sequence, as RNA have U in place T. Please clarify.
Thank you so much sir for providing such a great information & I think this crispr cas9 system will bring a revolution in the field of physiology & medicine.
Its so amazing that you know so many topics. I would like to learn more about how the bacteria distinguish the viral genetic materials from its own? especially after it incorporates into the bacterial chromosomes?
Sir I have a question. Cas9 can cleave double-stranded DNA, so we can design our crRna based on any one of these two strand information. Right? which is, as u said, the Protospacer. Then how the crRNA will bind to the protospacer? because the crRNA is almost identical to the complementary DNA sequence of the Protospacer, which is already Hydrogen bonded with it. Won't steric hindrance come into the picture here? you have drawn the target region like a replication bubble. Does it mean that the hydrogen bonds are broken at that particular place?
Yes, obviously. The interaction of crDNA with the target DNA requires site specific melting of Double Stranded DNA, in other words, a 'replication bubble' like structure will form due to the combined action of DNA Helicase and Single Strand Proteins. Thank you.
You are the sole individual responsible for me wanting to continue science and I mean it! Since I came across your videos back in 2019 it has changed my perspective entirely! I’ve grown fond of my subjects due to clarity of concepts and my grades improved massively straight from a 9.00 GPA to 9.88. All thanks to this man right here!
I am really honoured. Proud of you
9.00 seems rather high to begin with...
@@taladiv3415 Haha I’ll take that as a compliment, so there were so many students scoring similar or better that it wasn’t a deal at all, after the jump I could atleast make it to some rank if not the 1st.
@@zxyz786 Now you paint a clearer picture.
@@shomusbiologyofficial sir please make video on cytosine base editing and adenine base editing .please its request
I used to learn things from your video during my neet preparation and now I am Watching for my 1st yr mbbs final exam, thank u so much sir☺️
Glad to hear that you're getting benefit from my lectures
Dear sir,
I used to watch your videos during my MD residency (Biochemistry). (2014 -17)
and since I am watching your all videos regularly.
Your technique for teaching and your fundamentals direct set and hit in mind.
after so long I want to say thank you.
THANK YOU.
Edited:
I bookmarked your few videos as a important one for examination as well as for reference.
I am glad to hear that you're getting benefit from my lectures
Excellent uj.
@@shomusbiologyofficial So the virus (phage) can never kill bacteria if they have CRISP mechanism on them, right?
What happens if the adaptive immunity has no S sequence already present for the new viral DNA injected inside the bacteria/microbes?
One of the Finest teacher I have come across. Tq for helping budding life science students to get there concepts crystal clear.
Thank you so much for appreciating my efforts
Sir i am a veterinary student, i done my biotechnology portion under veterinary microbiology fully from your videos.
You are such a fab teacher and i am glad to be your student through this virtual youtube mode.
Stay tuned to know more
You are such a blessing to the people who really want to understand the complex topics. Keep doing this.
Thank you so much for appreciating my efforts
Instant subscribe, I've watched a bunch of videos on cripsr before this but no one started with basics and the way u explained is god teir 🛐
Thank you so much sensei
Thank you for appreciating my efforts
U literally saved me sir!.... I m struggling with this topic since last 2 days & after watching ur video the topic is very clear to me... Thank you so much 😇
All the best
My ug results declared, I'm so happy today. Thank you sir being a great teacher
Congratulations.
Nee njan udeshikune aal aano
You’re brilliant, and an excellent teacher of all you address. Much respect!
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thank youuu for uploading this video!! Currently I’m using crispr cas for my masters project
You're welcome. Glad to hear that you're getting benefit from my lectures
@arslan ahmad how can I help you
I am very interested CRISPER Cas9 and think we go to this topic for Ph.d
Tu contenido es increíble, tanto que una profesora de mi Universidad usa tus videos como referencias para aclararnos temas, sigue así.
😊😊😊😊😊👏🏻👏🏻👏🏻👏🏻👏🏻👏🏻
Dear Shomu sir,
Just want to let you know that your lectures are loved by everyone who's into a field related to biotech. I am a student from IIT Kanpur and I always watch ur videos before my exams. Thank you!
Thank you so much for appreciating my efforts. I am glad to hear that you're getting benefit from my lectures
I want to be like you. I love the way you teach. You are an inspiration to all of us ❤️
Thank you so much for appreciating my efforts
Sir ,
I am is class 12th
I am reading my biotechnology chapter and same time I get information about the Nobel prize given and started to get information about it .
I got your video and it cleared my all doubt and now I am going to make project with using your applications of CRISPER discussed at last to present my school in online Science exhibition
Go ahead
@@shomusbiologyofficial Thanks sir
You are much much better than any professor of any university ❤️❤️❤️❤️
Thank you so much for appreciating my efforts
Thanks for explaining this complex topic in such a simplified way Shomu da!
You're welcome
There's no biology-related exam where I don't watch your videos! Really helpful .. I wish for more knowledgeable videos.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
The most easy and effective explanation of CRISPR u have given sir, both of ur videos on CRISPR-cas9 are very useful, thank you sir 😊💝
Glad to hear that you're getting benefit from my lectures
thank you sir for uploading this...after that nobel prize was in search to know this technique... and your vedios are most usefull for us...
You're welcome. Glad to hear that you're getting benefit from my lectures
always sir.... getting the benefit since i was doing bsc now i am in my msc 3rd semester and preparing for csir and your vedios are helpfull in many ways thank you
Crystal clear.....watching your videos makes a difference......much preferred than even any animation....thanks sir
You're welcome. Glad to hear that you're getting benefit from my lectures
Wanted this lecture from quiet a long time....Thanku sir
You're welcome. Glad to hear that you're getting benefit from my lectures
You are brilliant becoz of u I understand this topic
Thank you
Shomu's biology should also receive Nobel prize for helping so many students across the world!
Not worthy of the Nobel. Many more to serve
@@shomusbiologyofficial sir ek question that nei abhi Bsc 2nd year mei hu Muje padhnee mei mazaa toh ata h Lekin Muje ye nhi pta ki future mei mai kya krunga koi solution h toh bata dijiye.
I saw other videos and wasn't able to clearly get it..I came here and boom the concept is crystal clear..wow ♥️🔥
All the best. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial the reason fr my sgpa above 9 is truly coz of your lectures can't thank u more..🥹🤌🏻
Thank you sir..
I am waiting for someone to explain this highly discussed topic.. who else better than you!!!
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Shomu's Biology how can I get this drug I want to cure Hiv in my body
Sir I've been greatly benefited from your videos. Recently I'm watching the biochemistry portion. Thank you so much
You're welcome. Glad to hear that you're getting benefit from my lectures
Shomuda.. osonkho dhonnobaad..
Thank you
you are doing a Commendable job. Super! You've got your brain in gear today. We all are proud of you and proud of your efforts. I just want to request you that stay safe and take care.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
U are amazing teacher. Even i have ni words for ur applouse or appreciation keep rocking in teaching.
Thank you so much for appreciating my efforts
good lecture :) I am doing Ph.D. and doing research work on CRISPR/Cas9 (Suppress the viral effect in plants via CRISPR {Multiplexing})
Thank you so much for appreciating my efforts
Can you suggest me a topic under crisper cas9 for phd proposal
of course. actually, I am working on a plant virus. what is your interest? I will guide you
@@SidraLively hi i am intrested to work in cancer biology crispr editing ti target cancer cell this is my rough idea
Plz suggest me something better if or help me to prepare a proposal
@@sahadiyamandodan2228 yes CRISPR can...you can also other viral diseases like dengue, Covid, hepatitus...
Thank you so much for your videos. Your explanations are always very helpful and allow to understand even difficult processes. Thanks to your visualizations I can finally remember everything. You're an excellent teacher!
Extremely well explained. Daruun shom... as a PhD dekhlam and khub bhalo bolecho
Thank you
Many many Congratulations to both ladies 🎉
Great
Thanks a lot . You have done a great job for a long term which help us a lot.
Thank you so much for appreciating my efforts
Thanks Again Shomu, incredible content as always my friend.
Thank you
I am here after getting to know about Nobel prize and literally it is very very informative 👍 Thankyou
Nobel prize 2020 in chemistry
Yes. Crispr cas9 rocks
@@shomusbiologyofficial sir I love this technique but I have a question .. why nobel prize in chemistry ?? Not in physio n med ??
@@ankitarora22 Probably because it is a technology,yet to be widely used as a medicine.
@@bhaskarjyaachatterjee may be ... Committee should make another category I guess
This is a Great Gift For Human Kind..
The Scientists Who Invented a Sharp tool,Of Drafting of DNA , Immanuel Charpientier, And Jennifer Doudna
Are Next Level Angels in Medicine field.
They have finally won their well deserve prize!! So happy ♡
Yes. This is big
Such a wonderful lecture u gave... u made a complex matter into a very simple... really appreciate your work ...
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
You explained in the best possible way 🤩
Thank you so much for appreciating my efforts
Sir I'm watching your videos since class 11. I was really confused about my future education after 12th. You're the one Sir , who inspired me to take Biotechnology as my subject after 12th. In the month of June , 2021 , My HS will be over. And after that I can pursue my dream Subject , only because of you Sir❤️.
Thank You So much Sir providing us these Amazing conceptual videos on almost every aspects of Modern Biology.
Thank You again ❤️🔥
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
are u doing buotech??
Thanks again for teaching this topic ..😊🙏🙏🙏 finally i understood the real concept..the beat teacher till date 🙏😊
You're welcome
It is explained wonderfully. Love from Pakistan.
Thank you so much for appreciating my efforts
Just one thing to say, Thank you dada❤
You're welcome
I was more like a " studying just to pass" student....then I came across your videos..I've topped my college in masters and now I've qualified my GATE.
I owe you my confidence in my subject 😌
I somehow have this urge to make you proud 😂
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
I’m in class 12 and I’m really interested in gentics tho it’s out of my syllabus. I found this vedio and it’s really interesting.Thanks sir for giving this very useful lecture. I have understand everything about CRISPR- Cas9🙌🏻❤️
Thank you so much for appreciating my efforts
Does the CRISPR RNA segment binding to the target DNA require the DNA to first be in its single-stranded form or how?
Thank you sir 🥺
I was in search of easily understandable blog or video on this topic and then I found it.
It helped me a lot ❤️❤️
Thank you so much for appreciating my efforts
Thank you sir
🙂🙏
You're welcome
Thank you very much sir for such a very valuable guidance, making it easily understandable, i found it very helpful for my seminar today,i got praised by my lecturers 😇
Glad it helped
Your videos are very helpful. I have a doubt. If the CRISPR is an RNA template, then how to binds to DNA having complementary sequence, as RNA have U in place T. Please clarify.
Thank you so much sir for providing such a great information & I think this crispr cas9 system will bring a revolution in the field of physiology & medicine.
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Is the Crispr cas9 system’s origin a specific bacteria and if so what bacteria is it? K12ecoli?
Ur world most famous and wonder full teacher to me,gurubrahma,guruvishnu,gururdevo, guru shaaakshat parabrahma tasmaisree gurave namaha 🙏🙏🙏🙏
Thank you for appreciating my efforts
You are a saviour
Thank you Sir!❤❤
You're welcome. Glad to hear that you're getting benefit from my lectures
Well done! Good job.
Still I have some doubts but i will manage them!
Glad it helped
Very informative session. Fantastic vid. Thanks a ton for this.
Thank you so much for appreciating my efforts
Always satisified! Great videos! Keep it up.
Thank you so much for appreciating my efforts
Very very helpful and excellent explanation
Thank you so much for appreciating my efforts
Sir,
Thanks for your clear explanation. Great work.
Thank you for this amazing and clear explanation for this topic.
You're welcome. Glad to hear that you're getting benefit from my lectures
Wonderful explanation
Thank you
Too good Sir...
Thank you
Thank you sir...for uploading the video..it clears the topic
You're welcome. Glad to hear that you're getting benefit from my lectures
Wonderful explained sir ....
Sir 1 ques.... what makes crispr RNA different from normal RNA??
Learnt something new..dhonnobad Sir👍🏻
You're welcome
Wow sir….wonderful ❤❤
Thank you
Thanks for making these videos
You're welcome
VERY WELL EXPLAINED!!!
Thank you
This was helpful. Thankyou.
You're welcome
you make learning interesting
Thank you
Thank you so much sir...
For make these concept clear.
You're welcome. Glad to hear that you're getting benefit from my lectures
Its so amazing that you know so many topics. I would like to learn more about how the bacteria distinguish the viral genetic materials from its own? especially after it incorporates into the bacterial chromosomes?
Nice explanation
Thank you
very beautifully explained thanks
You're welcome
Finally got the Nobel prize
Yup
You are amazing sir literally amazing...
Thank you so much for appreciating my efforts
VERY INFORMATIVE LECTURE
Thank you
How beautifully explained ❤️
Thank you so much for appreciating my efforts
very thanks sir u teach lot well.
Thank you
great video thank you so much!
You're welcome
Sir please make a video on Haemocytometer.
thanks so much for update our knowledge keep up the good work
You're welcome. Glad to hear that you're getting benefit from my lectures
Good explanation keep doing these stuff please
Thank you. Glad you liked my lectures
Good work sir
Much respect and wish you the best thank you.
Thank you so much for appreciating my efforts
Excellent explanation!!
Thank you so much for appreciating my efforts
Very useful vdo. Thank you so much. I can understand it clearly.
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank u so much for the wonderful explanation
You're welcome
What a explanation 🙏🙏🙏 thank you
Thank you
Love the videos, please do consider getting a mic though.
Mic has been upgraded since
Thanks for an excellent presentation. You mention that CAS nuclease activity is shut down. What is the mechanism to shut down this activity?
Great stuff
Very informative...
Thanks sir!
You're welcome. Glad to hear that you're getting benefit from my lectures
Dear sir,i want to know how the specific inhibition of transcription is done by crisprcas9 complex
It's mentioned in this lecture itself
@@shomusbiologyofficial actually i didn't get this from lecture. That's why i asked you.
Sir I have a question. Cas9 can cleave double-stranded DNA, so we can design our crRna based on any one of these two strand information. Right?
which is, as u said, the Protospacer.
Then how the crRNA will bind to the protospacer? because the crRNA is almost identical to the complementary DNA sequence of the Protospacer, which is already Hydrogen bonded with it. Won't steric hindrance come into the picture here?
you have drawn the target region like a replication bubble. Does it mean that the hydrogen bonds are broken at that particular place?
Yes, obviously. The interaction of crDNA with the target DNA requires site specific melting of Double Stranded DNA, in other words, a 'replication bubble' like structure will form due to the combined action of DNA Helicase and Single Strand Proteins. Thank you.
Cas9 protein has nuclease as well as dna helicase activity. So it opens up the target region before crRna can bind to it.
Happy teachers day sir 🙏🙏🙏🙏
Thank you
thank you this video is very helpful !!
You're welcome. Glad to hear that you're getting benefit from my lectures
you are my best teacher. lots of love from a fan and you are looking handsome in long hair.
You're welcome. Glad to hear that you're getting benefit from my lectures
Sir i have a confusion that how this crispr rna is regulating the transcription of the desired dna sequence.