I watched many videos about flow cytometry and this one is the best. Your way in explaining is much clearer than even flow cytometry articles! Thanks a lot. Please do more videos about flow cytometry.
"Whenever you discuss compensation you'd better lock the door before when you start talking about this because people will just run away. They will try to escape" That part made my day.
I had this exact training a while ago at my former company! Dr. Epron explained these concepts very clearly. I'm glad I found this on UA-cam, good to recap the basic insights!
This is very helpful to me. I'm new to the instrument. with this presentation, I am able to decipher some of the theoretical aspects of cytometer. Thank you for putting this.
Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer? This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
Hello range, depending on the capabilities of the flow cytometer, this is possible. Assuming your cells express antigens what can be bound through your PE and FITC labeled antibodies. If you really want to use two different lasers to sort the cells, you would need a cell sorter with a blue (to excite FITC) and a yellow (to excite PE) laser. After displaying your data with PE at one axe and FITC at the other in a so called “dot plot”, your PE pos, FITC pos cells would appear in the upper right corner and could be chosen for further cell sorting. If you just want to analyze and not to sort the cells with two different lasers, the MACSQuant® VYB is equipped with a blue and a yellow laser and would excite PE and FITC perfectly. But and this is important, if you just want to sort or analyze PE pos, FITC pos cells, it is not mandatory to use two different lasers. Both PE and FITC could also be excited with the blue laser alone but the signal would be detected through different detectors. Therefore the computer can still distinguish and sort the cells with our MACSQuant® Tyto® Cell Sorter for example.
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I've seen many tutorials and read many articles on Flow cytometry, you've made everything crystal clear. Miltenyi should be proud of having you.
I watched many videos about flow cytometry and this one is the best. Your way in explaining is much clearer than even flow cytometry articles! Thanks a lot. Please do more videos about flow cytometry.
You are a flow cytometry genius. A remarkable way of explanation. Please upload more videos on multicolour flow cytometry.
"Whenever you discuss compensation you'd better lock the door before when you start talking about this because people will just run away. They will try to escape"
That part made my day.
Glad you stayed in the room @Frank 😉
Trust me, you are not alone. Lessons learned......
The best webinar I have ever watched. Thanks a lot!
I had this exact training a while ago at my former company!
Dr. Epron explained these concepts very clearly.
I'm glad I found this on UA-cam, good to recap the basic insights!
an amazing presentation, I would like to ask for more presentations like this, your employer is lucky to have a brilliant scientist like you!
This is very helpful to me. I'm new to the instrument. with this presentation, I am able to decipher some of the theoretical aspects of cytometer. Thank you for putting this.
Great seminar, thank you!
Excellent talk.
Great explanation!
Thank you so much for that extensive explanation. Really just what I needed
Fantastic!
Great presentation as far as physics-related discussion.
Excellent !
thank you so much! amazing explanation
Glad you liked it!!
So helpful, thank you!
thanks a lot. This video is great help.
Thinking on write a good book on cytometry? Please go ahead!! State of art presentation. Liked a lot RAE Affinity.
Brilliant presentation
Best best best!!!
thank you.
very nice!
Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer?
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
Hello range, depending on the capabilities of the flow cytometer, this is possible. Assuming your cells express antigens what can be bound through your PE and FITC labeled antibodies. If you really want to use two different lasers to sort the cells, you would need a cell sorter with a blue (to excite FITC) and a yellow (to excite PE) laser. After displaying your data with PE at one axe and FITC at the other in a so called “dot plot”, your PE pos, FITC pos cells would appear in the upper right corner and could be chosen for further cell sorting.
If you just want to analyze and not to sort the cells with two different lasers, the MACSQuant® VYB is equipped with a blue and a yellow laser and would excite PE and FITC perfectly. But and this is important, if you just want to sort or analyze PE pos, FITC pos cells, it is not mandatory to use two different lasers. Both PE and FITC could also be excited with the blue laser alone but the signal would be detected through different detectors. Therefore the computer can still distinguish and sort the cells with our MACSQuant® Tyto® Cell Sorter for example.
Really very helpful..
!!