Gene Knockout using CRISPR

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  • Опубліковано 9 лют 2025
  • CRISPR technology democratized genome engineering. This game-changing breakthrough makes it feasible for every researcher to perform gene knockout without any major undertaking. To make things even easier, OriGene provides a pre-designed knockout kit for every human and mouse gene. Each kit includes all the plasmid constructs (2 targeting gRNAs, one scramble gRNA, and a donor template vector) and a simple protocol. This video tutorial serves as a step-by-step guide for the gene knockout experiment.

КОМЕНТАРІ • 18

  • @LouiesLog
    @LouiesLog 2 роки тому +3

    Gene knock out is the coolest name in anything biology

  • @Ahmad-yv5mo
    @Ahmad-yv5mo 3 роки тому +1

    what are the three ways to upregulate the expression of a gene in arabidopsis using the CRISPR/Case9 method...please help me in this

  • @zahnpastacremetube
    @zahnpastacremetube 2 роки тому

    Why is the PGK promotor used specifically? What are the specifics, the advantages compared to other promotors?

  • @malanggul215
    @malanggul215 2 роки тому

    Can we cure the cml ph+ with the help of crisper cas9? Please answer

  • @funny11744
    @funny11744 6 років тому

    Please help me for a CRISPR -project :homozygous or heterozygous of a point mutation of a certain gene - involve different approaches ?

  • @marcpadilla1094
    @marcpadilla1094 2 роки тому

    Upstream? What goes on in DNA. What's reading and activating all our DNA on a daily basis. Brain waves or what. Some quantum physics, Super position, entanglement, binary functions.

  • @ФилипЈанг
    @ФилипЈанг 3 роки тому +3

    Very difficult for understanding...

  • @chhayachandran4773
    @chhayachandran4773 3 роки тому

    Can i wrk in ur lab do you have career opportunities?

  • @zahrazamani2810
    @zahrazamani2810 4 місяці тому

    Is there any hope for those children who have not received oxygen and are paralyzed to walk and regain health?

  • @TariqAziz-sg9fl
    @TariqAziz-sg9fl 3 роки тому

    is this in ppt hard form available ?

  • @sandraoza675
    @sandraoza675 3 роки тому

    Why are there 2 gRNAs in the kit when only 1 is used?

  • @TheQueen-zr7uy
    @TheQueen-zr7uy 3 роки тому

    اريد ترجمة للفرنسية

  • @anythingsahaj7467
    @anythingsahaj7467 5 років тому +3

    poor audio and visuals... good content.. needs to be improved

  • @sarcastamusraconteur6881
    @sarcastamusraconteur6881 8 років тому +8

    he said cleavage....

  • @earthworm-filledstomachbyc4254
    @earthworm-filledstomachbyc4254 4 роки тому +1

    Seek Christ Jesus YHVH ,