DNA cloning

DNA cloning animation - This lecture explains about the DNA cloning techniques with vectors. The molecular mechanism of DNA cloning is explained with animation.
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Source: Lodish, et al., Molecular Cell Biology, Fifth Edition, W. H. Freeman & Co.
© 2004 W. H. Freeman & Co., and Sumanas, Inc.
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Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.The use of the word cloning refers to the fact that the method involves the replication of a single DNA molecule starting from a single living cell to generate a large population of cells containing identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.
In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically-modified microorganisms (GMO).
Link- en.wikipedia.or...

[@basantalsayed3127]

this video explains in 4 minutes what my professor couldn't in 1:47:34 of awkward lecture
officially Shomu's Biology Is one of my references

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+Basant Alsayed thank you. Glad you liked my lectures

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Big facts professors can be dumbasses.

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like literally!

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Shomu's Biology- words cannot describe how helpful you have been in my undergrad so far as a Bio major. Thank you soooo much

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[@kathyweeds]

Thank you! I could not visualize the technique for my exam. Pics were not enough. Your moving example is perfect. Keep it up!

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+Kathy Eich thank you.

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Kathy Eich
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Shomu's Biology )

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Yes i totally agree with you.

[@Hoid.]

Thank you so much! This was so clear and understandable, my teacher spent 2 hours on this and managed to confuse me more than anything

[@ayasamer205]

10 years ago and still very beneficial and amazing job
Thank u soo much.. u made really alot for us❤

[@shomusbiologyofficial]

All the best

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Best biology channel on UA-cam.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

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Remind me to come back to this in exactly a year when I have my next bio chem midterms

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[@shomusbiologyofficial]

There are videos on population genetics in my channel. You can find it easily.

[@1987minimum]

Got it, thank u

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Helpful video sir.. thanku somuchhhh... please upload more such videos 👍

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@shafiyahasan3275]

@@shomusbiologyofficial you're welcome sir...keep it up👍

[@dishninuwandika4907]

Thank you so much for this wonderful vedio. It's very important and useful for our studies

[@shomusbiologyofficial]

You're welcome

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woow best video to explain many topics at once

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This is the best video, no cap

[@shomusbiologyofficial]

Thank you

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cap

[@_royal.girl_3084]

It help ful for me.... thanku !!

[@shomusbiologyofficial]

You're welcome

[@_royal.girl_3084]

😊

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thanks Bhai....sabse mushkil topic h ye book ka....but from now it become easy.......keep going...

[@letsstudytogethernow]

luv u shomu! it was helpful

[@shomusbiologyofficial]

You're welcome

[@Dhruba_Banerjee]

Thank You very much. Helped me to understand the significance of selectable markers. Please prepare a separate video on selectable markers & cloning site too for me at least. I am giving XII boards in 2014. Urgently need it. Thank You.

[@shomusbiologyofficial]

You will find those topic under the "cloning vector" video.Watch those video.

[@Dhruba_Banerjee]

Thanks

[@taaptee]

this is beyond helpful, thank you so much

[@shomusbiologyofficial]

You're welcome

[@mushtaqhussain6121]

Nice lectures.... With digramic process

[@uzmashaikh1007]

Nice video.. Its really very helpful video...

[@shomusbiologyofficial]

You're welcome

[@williamjayaraj2244]

Thanks for this video.

[@sababashir12]

Very helpful 👍

[@shomusbiologyofficial]

Thank you

[@hjraoy]

thank You very much. Helped me to understand DNA cloning

[@sm247]

and you dont now before that video?
يعني انت كنت لاتعرف قبل هذا الفيديو ؟

[@mattiatorri1791]

i thought it helped you to make a cake

[@babbirajan1867]

My teacher is teaching still now but I didn't understood....
But you make it clear

[@shomusbiologyofficial]

You're welcome

[@anilmeena_02]

Awesome video 👍

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@nadeemnawaz6859]

WoW! Fabulous

[@ily2PM]

simple and to the point!! love it!

[@64.sreejithreju2]

Brilliant sir

[@shomusbiologyofficial]

You're welcome

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God bless you 😇

[@shomusbiologyofficial]

You're welcome

[@maltisaini7584]

Very helpful sir thanks for making this

[@shomusbiologyofficial]

You're welcome

[@ayushsinha2643]

Your videos are really very helpfull!! But please try not to show pop-ups while the video is playing. It covers the screen and is very distracting!!!

[@Bobas__2]

am i the only one who is fascinated by the concept?

[@ppwithaweewee9403]

Trust this is magic for me

[@mattiatorri1791]

yes

[@boikoketsoramokolo7616]

wow this was very helpful... Thank u very much

[@shomusbiologyofficial]

+Boikoketso Ramokolo you're welcome. Glad to hear that you are getting benefit from the videos

[@sahoogoutam]

able to understand very well

[@sahilmakkar6699]

very helpful videos ....thnx....god bless u

[@jvicism7135]

Thank you so much for the video. It really helps put everything I've read for class together.

[@MrAbuse96]

Very clear thanks

[@shomusbiologyofficial]

You're welcome

[@sijowdabdo8421]

Thanks for helpfull information

[@laraelzaher1914]

Thanks a lot u helped us ❤️❤️

[@MetoMailey]

I got it now .. Easy & Simple ♥

[@medicinestudent8349]

Thank you!

[@shomusbiologyofficial]

You're welcome

[@imjohnsteve2715]

Lol IAM grade 10 student even though we don't have this lesson in GRADE 10 IAM using this for research paper lol😂😂👍

[@shomusbiologyofficial]

Great

[@jacquelinekang75]

Thank you very much. This video is very helpful!

[@shomusbiologyofficial]

+Jacqueline you're welcome

[@bhagwatbhattarai705]

great video

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Thank you so much! This video gave me a lot of clarity :)

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Thanks 💚🇮🇳

[@meowismeify]

Thank you!! This helped!

[@ghadeeralmlek6394]

Thanks alot.. Can you maka a tutorial video about cell banking plz..

[@anshulrana5614]

Thanks sir

[@shomusbiologyofficial]

You're welcome

[@miss.beeslibrary220]

Sir pls can you make a video on trade secret laws of human cloning 😪 even their is no data on net.

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OG Shomu

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This was good!

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Wow 3 hrs to 4 mins.

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Sir amar kichu bisoi janar ache-structure-toxicity relationship,drug design,DNA binding,biological activity of metal ligand complex..ai 4 te tpic er opor video banale khub help hoi..

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"The Zeta Reticulians want to know your location"
(Great video by the way )

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I understand in 4 minutes.

[@shomusbiologyofficial]

You're welcome

[@manar-nasser]

thank u sooo mutch

[@shomusbiologyofficial]

You're welcome

[@proloyghosh.]

Please suggest a book for biotechnology so that I build up my concept regarding this. Please sir

[@sobster123]

really really good.! :)

[@cinthialara386]

Great video 👍 by the way,you can obviously clone prehistoric and current animals and edit DNA and that is very interesting to me an example would be to clone an arsinoitherium you must alter the elephant's DNA and the mix it with rhinoceros DNA to transform it into a prehistoric animal,dorudon alter the DNA of porpoises to turn them into(prehistorics whales) and finally megalania,a close relative of the komodo dragon alter the DNA of the Komodo dragon to turn it into a prehistoric reptile there is a chance that this will happen?

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Just wow

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@philanthropicnightmare1206]

I'm guessing some cells might take up multiple plasmids which could complicate things...

[@ddobrik6596]

it is complicated science

[@hassankhormi9076]

thank you so much

[@amalhosny]

great

[@natalieb.923]

So if there are many plasmids in the solution with sticky ends, what prevents the plasmids from recombining with itself for another plasmid instead of the DNA fragment you are trying to insert?

[@ppwithaweewee9403]

For now we go with we simply don't know. But one possibility is it might be happening but there are so many available and wayyyyy more genes available quickly, so the second the sticky ends are exposed BOOM! they go and hug. Tbh it's practically possible like science AIN'T clean in lab, like it's Portraited on paper stuff like this can happen. And there are countermeasures too. Hopefully i cleared your doubt.

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[@gtagamer757757]

so if we got a fragment of dinosaur dna and put it into a plasmids it could multiply and we could put it together and form a dinosaur?

[@nathalie7833]

Where would you get a usable dinosaur DNA?

[@wolves44445]

Well we don't have any dinosaur DNA, unfortunately

[@gtagamer757757]

well they have found fragments of dinosaur DNA in fossils

[@shwetakhade6531]

Yeah.. That's the principle they showed in the movies like Jurassic Park and Jurassic World.

[@anirbanghosal8427]

we just can't put it together to make dinosaur just like that...

[@EnosNjile]

is must the restrictions enzymes to cut dna at G and A not other site??

[@hardikgohil4190]

thank you

[@madeline_parks]

How do you determine which bacterial colonies have the gene of interest? Also, if you wanted only exons and no introns, wouldn't you use RNA and reverse transcription to produce complimentary DNA? Let's say you want to genetically modify an embryonic animal: Would you inject the bacteria themselves into the embryo or would you need to isolate plasmid DNA?

[@nathalie7833]

He said it, through doing an assay on each colony (for your first question). The bacteria is the host, if you want to genetically modify an embryonic animal, wouldn't the animal be your host? So you don't inject the bacteria, you inject the plasmid into a still growing zygote (okay I think doing it in a multicellular animal would be very much more difficult?)

[@mahamhaq1294]

which is a suitable vector to be incorporated with a large external DNA fragment? Small or large?

[@tessgallagher9738]

so could i do the same thing with dinosaur or over all animal dna or is do I ave to it it a different way?

[@proloyghosh.]

Multiple cloning site helps in incorporation of foreign DNA in biotechnology. But what is the usefulness of that site for bacteria itself??.
Dose all plasmid contains this site or it is constructed?
Please help me Sir.

[@shomusbiologyofficial]

We artificially construct these sites. Generally bacteria has some restriction endonuclease sites in their Plasmid or in chromosomal dna

[@proloyghosh.]

@@shomusbiologyofficial okay Sir. Thank you sir, thank you so much.

[@AhmedMD]

Thanks.

[@popflorian2991]

How the plasmid knows to replicate at the same time with the cell?

[@sheefaali7186]

Which book should I follow for RDT?

[@mattiatorri1791]

reverse dursed technique?

[@gurubabawithshiwani2433]

PlzzZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZ make another animation for cloning of liver cells

[@marianadeau8632]

I'd give it a thumbs up if I didn't have to close the million annotations every other second

[@theresepabroa4389]

you can turn the annotations off

[@kerryvp]

Gracias

[@David7hr]

I can,t follow his Indian accent. He should use a narrator and republish these excellent materials!!

[@shomusbiologyofficial]

+David C hi. I tried to improve my accent. You can catch my latest videos. Hope you can understand them.

[@lanoanhduongthi3536]

At the first stage i could not follow you but now I can follow almost what did you explain, my english also improved from you. very thank to you but could you please speak slowly for others they can follow you

[@mattiatorri1791]

now i have the ingridients to make a cake

[@shomusbiologyofficial]

Yes

[@altair2594]

smart!

[@Battery-kf4vu]

Would it be possible to add several alleles of the HTERT gene to the DNA of a spermatozoid and an ovum so that the resulting fertilized ovum could produce more telomerase and be immortal?

[@sanatdas2011]

Sir, thanks a lot for the video. I also need your help in my assignment submissions, I am currently doing my graduation from University of Calcutta, Department of Zoology. In my practical works I have a lot of pressure in third year, cannot really finish it yet. If you can kindly help me out then that would be of great help. Thank you.

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I get it now. so happy haha

[@sm247]

حلو |
nice |
good |

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Hi! Can you upload a video for Homopolymer tailing? thanks

[@laraelzaher1914]

Smooches 😘😘

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could we take any cell of our body.

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does anybody have a summary from this?

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That intro tho lol

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Sir, which came first,man or women?

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peace love ya'll

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1:25 owh like patching a new line.

[@enajlopez733]

how to download this?

[@jontesakify]

Hey is this narrator the same dude as in that the missile knows where it is shit?

[@Lazuli99]

can we code our dna to be immortal? I want to beat death

[@shomusbiologyofficial]

No

[@HimanshuRaj-ry2gx]

More like "chomu's biology"....

[@PitbullNamedCupcake-d1g]

Hi

[@viratjoshi4780]

What is need of qmplicilline in nutrient agar