This is the same question i received from someone in the comments on my Video on Total WBC Count. Answer is the same. So i've copy pasted the answer i gave here. Hope it helps. "The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
Basically to avoid double counting. Heres a good link www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right#:~:text=The%20point%20of%20this%20%22rule,between%20those%202%20squares%20twice.
To avoid double counting. Check this out. www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right
since i'm not a clinical lab technician by profession, i googled your question and mostly found that a separate special reticulocyte count is done only if the usual RBC count is abnormally high. Otherwise, RBC count generally do not discriminate between the two and are both considered in the RBC count.
Look on the smallest squares They are 16 on each square So,there are 5 biggest squares in which Rbcs should be counted having a total of 80 smallest squares inside(16×5=80) and there are also a total of 25 squares,the biggest ones so if you count smallest squares inside those 25 squares you get 800 smallest squares(25×16) so take; 80/400, you get 1/5 on simplification as the area counted.
Sorry for late reply. Dilution factor = Total volume taken (blood + diluting fluid) ÷ volume of blood sample taken. In this case , it is 100÷0.5. Dilution factor is simply the number of times the blood sample is diluted (20 times)
Tomorrow is my physiology practical
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~from India ❤️
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Studying for haematology exam tomorrow, thanks you sir. It's helped. MLS department.
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I am studying for tomorrow exams and after watching this video I understand the whole procedure in easy way Thank you🙏💕
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We zoologists practice this many times with patience just to get the correct outcome 😊
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My RBC count is 7.4 should i worry ?
The Blood test is all within normal rage except the RBC.
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Thanks for the nice presentation.
May I know, why I have to discard 1st 2/3 drops from Pipette, before CHARGING the chamber?
This is the same question i received from someone in the comments on my Video on Total WBC Count. Answer is the same. So i've copy pasted the answer i gave here. Hope it helps.
"The pipette is designed in such a way that the liquid in the long tube of the pipette does not take part in the dilution and mixing inside the bulb. So to get the most accurate result, the liquid within this long tube (first few drops) is discarded. Also the liquid in the long tube contains only the diluting fluid. All the cells have been sucked into the bulb. So the diluting fluid in the long tube cannot not take part in the counting and so has to be discarded. Hope that answered ur query.
@@TheSingtangpaScienceGuy a lot many thanks to you sir.
Apologies, because I missed that, "my question and it's answer" was already done!
Thanks again
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Bacteria
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Humerus anatomy
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Any physiotherapy student here.....
Me
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Very useful vdo👍🏻
Why we need to discard before putting the sample in the chamber?
2 hours Remaining from my Ospe/Viva And I am Watching this Video😂
nice explanation .well done
I can't seem to suck the blood. Do we have to hold our breath for a long time?
Wonderful.
How accurate is this approach to obtaining RBC counts as compared to automated methods.
Does this approach have any superiorities?
Human error is not involved in chemistry analyzers. Both are equally accurate as long as the manual method is performed meticulously
Why should we count RBC in smaller squares based on L rule?
Basically to avoid double counting. Heres a good link www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right#:~:text=The%20point%20of%20this%20%22rule,between%20those%202%20squares%20twice.
can't thank enough ❤️❤️❤️
Thank you sir.....♥️👌👌👌🥰
What did you dilute the blood with?
Ur explanation is too good and very helpful ,tq and keep it up
Which course is better in allied health sience
pls why just borders of the lower and left side are used for counting pls answer🌸
To avoid double counting. Check this out.
www.researchgate.net/post/During_cell_counting_in_a_hemocytometer_why_should_we_not_have_the_cells_touching_the_middle_line_at_bottom_and_right
@@TheSingtangpaScienceGuy thank youuuu🥰🌸🌸
@@TheSingtangpaScienceGuy pls can i have ur account to ask some quistion that realy i dont how to get la answer
Thanks 👍
Tq sooooooo much sir 🙏❤️
How can we avoid blood clotting while take the blood sample in pipette
Use anticoagulated blood
how we can find the value of "N"???
Hi, Please re-watch the calculation and formula part of the video. Its very clearly explained. N=total cell count in 5 grids.
What do we benefit from this test to find out what
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Thank you so much sir😊🙏🏼
ماذا نستفيد من هذا الاختبار لمعرفة ماذا ؟
Helpfull video 🙏🙏🙏
well explained sir 🥳🥳🥳
Thank you for the video
Sir my RBC level is 5.91 female is their any serious problem joints pain am facing 😢
Does RBC count shown in lab reports also includes reticulocytes?
since i'm not a clinical lab technician by profession, i googled your question and mostly found that a separate special reticulocyte count is done only if the usual RBC count is abnormally high. Otherwise, RBC count generally do not discriminate between the two and are both considered in the RBC count.
Can u tell me how u got area counted =80/400
Look on the smallest squares
They are 16 on each square
So,there are 5 biggest squares in which Rbcs should be counted having a total of 80 smallest squares inside(16×5=80) and there are also a total of 25 squares,the biggest ones so if you count smallest squares inside those 25 squares you get 800 smallest squares(25×16) so take; 80/400, you get 1/5 on simplification as the area counted.
we count the cell on upper line and on the left hand line. But u tell L rule wt is crtt sir
Any medical lab scientist students here?
G
🙌🏾🙌🏾
Yeah 😂
sir why are we diluting the cells ?
To prevent cell clotting
And facilitied to count ، without adhesion
Thank you 😊
Thank you sm!! 💗
how did u calculate the depth factor?
1/0.1mm
Thankyou so much sir
Thanks sir
Wow perfection!!!!
Thank you sir
Very helpful...
Best best best 👏👏👏👏👏👏
Thank you, the video was really informative
amazing.. 😄😄
Veterinary
17130 Denesik Throughway
Any nutrition and dietatics students 🥳🥳🥳
Awesome vedio...got more clarity .sir pls can I know how to find dilution factor...pls sir pls....I have exam sir
Sorry for late reply. Dilution factor = Total volume taken (blood + diluting fluid) ÷ volume of blood sample taken.
In this case , it is 100÷0.5. Dilution factor is simply the number of times the blood sample is diluted (20 times)
@@TheSingtangpaScienceGuy thank u very much sir👍🙏🤗🤗🤗
Sir can you explain me depth factor in detail...plz
Thanks sr
Thank you