ZINC FINGER NUCLEASES - GENE EDITING EXPLAINED!
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- Опубліковано 3 лип 2024
- This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
www.gla.ac.uk/people/adamwest
This presentation describes the architecture of zinc finger nucleases, which are engineered proteins used for genome editing.
This is part of a series (see playlist) that also covers
Transcription activator-like effector nucleases (TALENs)
CRISPR adaptive immune systems
Using CRISPR/Cas9 for genome editing
CRISPR specificity
Precision editing
Homology directed repair
Prime editing
This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.
Please leave a comment to let us know whether this was helpful to you and what you think we should cover next. We are a new channel so please give us a like and share our video on your social media if you think others should see it. We have a lot of content coming up, so please subscribe!
More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.
Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.
Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.
Publication List
1. Wah DA, Hirsch JA, Dorner LF, Schildkraut I, Aggarwal AK.
Structure of the multimodular endonuclease FokI bound to DNA.
Nature. 1997 Jul 3;388(6637):97-100.
doi.org/10.1038/40446.
2. Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK.
Structure of FokI has implications for DNA cleavage.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9.
www.ncbi.nlm.nih.gov/pmc/arti...
5. Wolfe SA, Greisman HA, Ramm EI, Pabo CO.
Analysis of zinc fingers optimized via phage display: evaluating the utility of a recognition code.
J Mol Biol. 1999 Feb 5;285(5):1917-34.
doi.org/10.1006/jmbi.1998.2421
6. Wolfe SA, Nekludova L, Pabo CO.
DNA recognition by Cys2His2 zinc finger proteins.
Annu Rev Biophys Biomol Struct. 2000;29:183-212.
doi.org/10.1146/annurev.bioph...
7. Klug A.
The discovery of zinc fingers and their development for practical applications in gene regulation and genome manipulation.
Q Rev Biophys. 2010 Feb;43(1):1-21.
doi.org/10.1017/S003358351000...
8. Urnov FD, Rebar EJ, Holmes MC, Zhang HS, Gregory PD.
Genome editing with engineered zinc finger nucleases.
Nat Rev Genet. 2010 Sep;11(9):636-46.
doi.org/10.1038/nrg2842
9. Carroll D.
Genome engineering with zinc-finger nucleases.
Genetics. 2011 Aug;188(4):773-82.
doi.org/10.1534/genetics.111....
10. Kim H, Kim JS.
A guide to genome engineering with programmable nucleases.
Nat Rev Genet. 2014 May;15(5):321-34.
doi.org/10.1038/nrg3686
Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(UA-cam Audio Library) - Наука та технологія
I am working on my paper analysis project for my undergraduate genetics course and this playlist has really helped me in grasping new concepts. Thank you so much for making this knowledge available to everyone.
You are very welcome. Best wishes for your studies. Stay safe, GG
These videos were perfect. Concise, thorough and incredibly helpful. Thank you!
Thank you Jenna. We're pleased that you found them to be useful!
Great explanation of an older--yet somewhat complicated-- technique. Really appreciate it !
Glad you found it useful. ZFNs still have merit as they are very small and easier to deliver in viral vectors.
This has been really helpful for my chemistry assesment at school. I personally found it very interesting and informative as well.
Great! Good link with your assessment
Thanks for the great resources you made. Very helpful :-).
Super helpful before my genetics exam! Thanks!
Glad it was helpful!
Excellent! Thank you
Glad you enjoyed it!
so touching for an excellent video
Glad you think so!
Superb concept So much Thank
Busy with my Honors in Biochem - this is pretty helpful
Thanks for the feedback Lara. Hope you find the rest of the series just as helpful!
This is incredible and invaluable, thank you. Just curious, when you mentioned that zinc finger arrays are small, I guess you imply that they are smaller than TALE and Crispr Cas 9 proteins. Without worrying about how the zinc finger arrays are made, does this "small" genome editing tool imply that the tool can be more effective in general, or are there cases such that only small enough tools like zinc finger array is effective?
Hello, what I don't fully understand is how by changing some aminoacids from the ZFN domain (each square) it is possible to recognize a specific trinucleotide.
Hi Javier, I suggest that you read reference 6 listed in the description or some other articles on zinc finger specificity. Best wishes
What's the colour pf zinc finger protein?
I really hope there's a Fok2 lol
Now crisper cas 9 available
Indeed. See our videos on them. ZFNs still have value as they are small proteins, which is good for viral vector delivery
Please help me sir do you tell me what is the source of nuclease in saliva of human
Goggle it! 😀
Gene and genome editing is the same or not???
'Genome editing' would include genes, gene regulatory regions, and other non-coding intragenic regions, so is just a broader term.
That means it's more or less the same thing right
The editing process is the same, the intended target is different
Ohh okay thank you so much ☺️
Good stuff Adam. I remember meeting you in Glasgow when I was in sales at Millipore. Now I work for Sangamo (via Sigma)!
Thanks. Hope everything is going well with you