Endotoxin testing : BET / LAL test

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  • Опубліковано 3 жов 2020
  • content : measurment of endotoxin using LAL TEST also known as bacterial endotoxin test
    Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts with bacterial endotoxin lipopolysaccharide (LPS), which is a membrane component of gram-negative bacteria. This reaction is the basis of the LAL test, which is widely used for the detection and quantification of bacterial endotoxins.
    There are three basic methodologies: gel-clot, turbidimetric, and chromogenic too.
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