for any poor soul who needs to find the 9 mistakes Labeled the top of the agar plate rather than the bottom. Did not keep the cotton tip of the swab sterile. Did not orient the swab correctly when selecting a single bacteria colony from the stock culture plate. Did not spread the bacteria colony evenly over the agar plate to create a lawn of growth. Did not discard the swab in the waste container. Moved the disc after it fell on the agar plate. Placed a disc too close to the edge of the plate so it was not possible to measure the diameter of the zone of inhibition. Dropped a disc on the benchtop. Did not space the discs evenly on the agar plate.
I like the idea of including food colouring, that's a great teaching trick to show the concentration gradient! I think my students sometimes struggle with visualizing this with a verbal explanation alone.
Thanks for not rushing through but putting it in simple and understandable terms. And that yellow glove scientist I found humorous though important as these are mistakes we could make but now we are alerted to be careful. thank you again and please keep helping students like us.
WOW the experiment now for me seems so easy after watching every step of it carefully and also the brief summary in the end made it even more illustratable to one's mind .thank you
I like your way of teaching please keep doing all these stuff about microbiology..... And if you have time please make some videos about its full details........... THANK YOU SO MUCH
thanks for the video. But, i want to ask something about the preparation of inoculum. I read the protocol of Kirby-Bauer Disk Diffusion Susceptibility and it said that the preparation mus be followed by 0.5 McFarland standard. It means we have to check the density of our inoculum according to 0.5 McFarland standard. On your video, you just pick one colony from the agar then spred it with the sterile swab. According to your opinion, which one is better for the preparation of inoculum? And how the differences between them? thank you
I have one quaries ,i have isolated endophytic bacteria from plant ,then pure culture ,and i want to test my isolated strain on pathogenic bacteria by MIC Method,but i dont know how to perform it.
Hi! I'm just a newbie. Just wondering how can I use garlic or other natural antibiotics for antimicrobial susceptibility test? I hope someone would answer.
You need to extract the juice from garlic or other natural sources, storing them in ethanol. Make a disc soaked with those extract at different conc. (e. g. 30/60/90 microL or as you want). After preparing the discs with those natural extract, simply follow this spreading method and put your discs on the plate. Search for papers or articles on this.
@@hassanalaa2112 @Hassan Alaa yea 1.labeling is wrong, with line writing. 2. contamination of swab. 3.wrong in striking of plate. 4. mistake in puting antibiotics in target place. 5. not handling of cup plate. 6. mistake in taking one colony. 7. forgeting small dot(.) on swab 8.upside down of plate that cause differentiation . 9. not adding another same antibiotic on plate. 10. not taking care of safety (like not putting swab in waste container).
@@hassanalaa2112 I found 6 mistake 1 It is mistake when picking the coloni 2 Contamination of swab cotton 3 false way to distribute 4 don't put swab cotton in recyclbin 5 antibiotic fall from forceps 6 growth of bacteria not true
Why in the world would you tell us there would be 9 mistakes and then not tell us what they were so we could see if we got the right one s?? That's actually cruel, you know !!!
for any poor soul who needs to find the 9 mistakes
Labeled the top of the agar plate rather than the bottom.
Did not keep the cotton tip of the swab sterile.
Did not orient the swab correctly when selecting a single bacteria colony from the stock culture plate.
Did not spread the bacteria colony evenly over the agar plate to create a lawn of growth.
Did not discard the swab in the waste container.
Moved the disc after it fell on the agar plate.
Placed a disc too close to the edge of the plate so it was not possible to measure the diameter of the zone of inhibition.
Dropped a disc on the benchtop.
Did not space the discs evenly on the agar plate.
Thanks from the future, you are a life-saver
I love you
@@livybearful omg, thank you! HAHA
OMG, Thanks So Much
You saved me!!! thank you so much!
I like the idea of including food colouring, that's a great teaching trick to show the concentration gradient! I think my students sometimes struggle with visualizing this with a verbal explanation alone.
Thanks for not rushing through but putting it in simple and understandable terms. And that yellow glove scientist I found humorous though important as these are mistakes we could make but now we are alerted to be careful. thank you again and please keep helping students like us.
Are antibiotic resistance test diagnosed by doing Urine for C/s test?
Best educational Concept Clarity.... Ur nature of Guiding is Excellent 👌 Now I can Study, rather than boring my Nuisance lectures
WOW the experiment now for me seems so easy after watching every step of it carefully and also the brief summary in the end made it even more illustratable to one's mind .thank you
I like your way of teaching please keep doing all these stuff about microbiology.....
And if you have time please make some videos about its full details...........
THANK YOU SO MUCH
do you know what are the 9 mistakes ??
U r just amazing.... crystal clear Concept. Thanks a lot madam🥰 amazing video
You explained it like a movie mam
It is so awesome hoping more videos
Thank you so much❤️
This was so helpful! Thank you!
This made the concept incredibly clear, thank you!!
Top-notch conceptual correlation and beautifully crafted and video. Thank you so much.
Heat, measurable scale, reference table, sterilization
So beautifully addressed this lecture ❤😊
U 're so amazing madam 🙌 u make it short but very much clear and educative too 🙏🙏
I'm looking for a lab idea (fast) and I was wondering if E.coli bacteria was used for this lab?
Thank you for doing this video!
I think you're an excellent teacher, also very pretty!
I absolutely love your channel, but what type of bacteria did you use for this lab?
Thank you sooo much! I could not visualize this by reading from my book!
I found this helpful ❤.
I would love to connect to a medical laboratory scientist here. I am a Nigerian 😊.
I'm learning this at school, For science and I find this very interesting!
Thank you! This video was so helpful!!
That's one engaging and informative video. Thank you.
I'm just a biggenner and my English is not very good but I understand this video it was so helpful thanks a lot 😘😘
What is likely to happen if antibiotic discs are placed on the inoculated media and left at room temperature for an hour before incubation?
Narrator: Label the bottom of the plate
Yellow gloves: how about no...
Whats the method of inoculation? Thank u
Great video. Super clear explanation!
Good morning great information sister
In result they mension "sensitive" what it is means
Great video thanks
Do we have to do this in a laminar flow hood?
I like this video keep going 🤠 greeting from Morocco
Mam provide more like multiculture on different samples on single media
I'm very new to Microbiology, but just about flipped when yellow grabbed the sterile cotton tip by the tip! Ahh!
So helpful 💯 Thank you so much
This so useful and help me a lot
Thank!
I very interesting the way u explain thnkz
Thank you!❤️
What about the 9 mistakes?
Educative, thanks for this
Great and helpful video 👍
How to prepare the antibiotic dics?
Thank you bae 😍
Very nicely done
What dye was used to show the diffusion zone?
Thank u so helpful ❤️
this was fabulous
Thank you it's quite interesting and helpful
Thanks for the video!
thanks for the video. But, i want to ask something about the preparation of inoculum. I read the protocol of Kirby-Bauer Disk Diffusion Susceptibility and it said that the preparation mus be followed by 0.5 McFarland standard. It means we have to check the density of our inoculum according to 0.5 McFarland standard. On your video, you just pick one colony from the agar then spred it with the sterile swab. According to your opinion, which one is better for the preparation of inoculum? And how the differences between them? thank you
yeah...I am agree to you...
هذا شرح يخبل وشغل مفصل مو مثلنا كضينا كل أسبوع تأجل الشغل عالورا 🚶♂️💔
تشکر .مرسی.tankeyou
Why is she hating on scientists who wear yellow gloves? -_-
They have messy handwriting
do you know what are the 9 mistakes ??
Thank you sooo much!!!!
Thank you so much, really helped with my homework! :)
do you know what are the 9 mistakes ??
Great and helpful.. thank you
do you know what are the 9 mistakes ??
what if a disc does not have a halo over it ? does it mean it is resistant to the antibiotic?
yes
I have one quaries ,i have isolated endophytic bacteria from plant ,then pure culture ,and i want to test my isolated strain on pathogenic bacteria by MIC Method,but i dont know how to perform it.
what is the name for the antimicrobial disc with the code W 2.5 ?
Thank you
Very interesting.
Beautiful 🥰🥰
what are the aseptic techniques that were used
Очень полезное видео, спасибо большое!
Thank you for your video
Wait, how about the 9 mistakes?
Same,
What are the 9 mistakes?
I also want to know the 6 errors in the video
There are a total of 9 mistakes. If you actually watch the video, you will see the mistakes. She basically points them out for you.
Stooopid
@@BrittanyTeague12398 do you know what are the 9 mistakes ?? just say it briefly... p.s: i spoted 6 mistakes
Ur mom
Awesome!
muito obrigado!!
Do you use tetracycline in different dosage for six time??
Or different antibiotics for six time??
Someone explain me
She used 6 different antibiotic, but here we can use one antibiotic with six different concentration
@@drhumajawed7536
do you know what are the 9 mistakes ??
Hi! I'm just a newbie. Just wondering how can I use garlic or other natural antibiotics for antimicrobial susceptibility test? I hope someone would answer.
You need to extract the juice from garlic or other natural sources, storing them in ethanol. Make a disc soaked with those extract at different conc. (e. g. 30/60/90 microL or as you want). After preparing the discs with those natural extract, simply follow this spreading method and put your discs on the plate. Search for papers or articles on this.
@@mostafijurrahmanalhadi5292 thanksssss
I like your question , impressive
@@mostafijurrahmanalhadi5292 Men I had the same question and you answer it perfectly
Excellent
that was amazing, thank you!
Thank you Soooo much
So nice
do you know what are the 9 mistakes ??
perfect..thks so much
To grow the bacteria no meed for a specific medium right? Just wonderning
thanks for this type of vedio
i love it.
do you know what are the 9 mistakes ??
@@hassanalaa2112
@Hassan Alaa
yea
1.labeling is wrong, with line writing.
2. contamination of swab.
3.wrong in striking of plate.
4. mistake in puting antibiotics in target place.
5. not handling of cup plate.
6. mistake in taking one colony.
7. forgeting small dot(.) on swab
8.upside down of plate that cause differentiation .
9. not adding another same antibiotic on plate.
10. not taking care of safety (like not putting swab in waste container).
Thank u
It is interesting Video thanks,😇
Well Explained
do you know what are the 9 mistakes ??
procedure explain in english
Biochemical test
thanks a lot
do you know what are the 9 mistakes ??
Good job 👏
do you know what are the 9 mistakes ??
@@hassanalaa2112 no please
Tell me what they are
Pls make more video
Thanks exam passed
do you know what are the 9 mistakes ??
@@hassanalaa2112
I found 6 mistake
1 It is mistake when picking the coloni
2 Contamination of swab cotton
3 false way to distribute
4 don't put swab cotton in recyclbin
5 antibiotic fall from forceps
6 growth of bacteria not true
Great video.
do you know what are the 9 mistakes ??
thnks ma'am.
great d helpful thank you
Why in the world would you tell us there would be 9 mistakes and then not tell us what they were so we could see if we got the right one s?? That's actually cruel, you know !!!
Nice
do you know what are the 9 mistakes ??
thanks madam
Thans
D diomand Test
Gr8 madom
👍selamun aleykum kolay gelsin başarılar dilerim kalıcı üye olalım inşallah iyi gunler
👍🏽
Anyone from DPMI 😂🔥
quite funny