No matter how much time i study in one topic, how many videos i watch on it , i need your video at any cost for my clear understanding and mental satisfaction... Thank you
Thankyou so much sir , our lecturer teaches us like we’ve already gone through the topic 10 times , he never clears basics and only goes on his lecture saying you already know you although know If it wasn’t for you I couldn’t have passed
You are amazing! You have been saving me throughout my undergraduate years. Thank you so much for everything you do for students all around the world. :)
I am a university student in America and they way you explain things most of the best professors in my school cant explain it the you do! I would listen to them for hours and not know what they are talking about. But with you, I just cant not understand it!!!
Yesterday my teacher expalined this topic but i am confused many time so just search about electrophoresis and your video is found......thank you for helping to understanding in this topic
Thank you very much Sir...these two videos are more than enough to clear our confusion on gel electrophoresis..I wish to know more about Gradient Gel Electrophoresis from you...also watched your lecture on 2D gel electrophoresis...that was also very much interesting...Honestly speaking Sir...you are an Encyclopedia to us...on all these topics on Biology
Thanku sir .... Not only i but every one pray for u... becoz lot of students gain more nd more knowledge through ur video that iz most helpful for our furture .the most thing iz that lot of students that are poor like me can pass their exams easily without any problem
Hai sir After watching ur lecturings sometimes without knowing myself, my eyes got filled with tears, equally am learning and feeling ur lecturings🤗 Thank you my Bhrahma 🙏♥️♥️
Thank you so much sir..for ur efforts... keep it up...n love r style of presenting n discussing...these always save my time..n make my concept 99% clear..
Sir i like your videos so much.I like thid one too.....i was unable to understand SDS -PAGE . I search for book too but did not find out.but your video helped mee alot!....pls also make video on detailed applications of modern technquies.....it will be so kuch helpful.🙂🙂🙂
Sir I had one doubt In SDS page all the proteins get negative charge from the SDS so that they can easily move towards anode, but in native page there is no charge donator like SDS then how positively charged proteins gets seperated?
Hey! Always thankful for your stuff, incredibly educative. I have a question. Ive been studying 2DGE lately, and my professor always stresses that you need to prepare the sample for the MW based separation with iodoacetamide to remove S-S bonds, but this seems to do the same thing as B-mercaptoethanol, does anyone know why doing both of these things is important, and why Iodoacetamide does not suffice on its own? Thanks!
2.5 mAmp current is required in case of semidry transfer. Not in case of SDS-PAGE. Rather after SDS-PAGE the 2.5 mAmp current is used to transfer the proteins from gel to the nitrocellulose or PVDF membrane in case of semidry transfer process only.
Thnku sir for your great effort towards us , u make us understand many things , but sir I am really confused to follow which video of shomus biology , means this one or one of your old video , can u plz recommend
Also I have one more question that as we use tris glycine buffer as running gel in sds. The glycine enters the gel and help in lining of the proteins before entering the resolving gel. Is it possible to run sds if we don't use glycine.
Great video! I have a question- in one of my experiment, I noticed that a diner protein (44kda) was migrating slower and was found at 50kd. This dimer has positively charged amino acids. Can you please tell me what could be the possible reason for slow migration of this protein?
How many polypeptide bands detected on gel, if we electrophorised secretory IgA on SDS-PAGE under reduced and denaturing conditions. Sir, plz answer urgently 😔😔😔😔
Can we separate RNA by Agaros gel also? And if we have mixture of DNA snd mixture of RNA in gel, who will being faster? DNA or RNA Or we not compirsion DNA and RNA togather? Just DNA size it self and RNA itself?
In native gel, we don't denatured the protein. It moves as it is in structure and in denatured gel, we denatured proteins and then allow it to migrate in gel
Sir really really thank you so much 😊 Sir mujhe 1 Question puchhna h SDS-PAGE m do Ph Q lete h Resolving Gel & Stacking Gel k lie ? Please sir please please reply with d answer. I have to answer this Please sir
Sir plz refer any molecular book related to techniques e.g electropheris, sds, fish qrt pcr hybridization Bloating pcr etc plzzzzzzzzzz told the author name of that book your lecture is good thanx keep it up
SDS-PAGE is also a discontinuous gel system made up of two different pH gels called stacking gel of pH 6.8 and separating gel of pH 8.8 which also differ in its pore size. You have not mentioned this important point
I love your lectures. you are the reason why I got an A in molecular pathology. I really appreciate your help!!
I am glad
No matter how much time i study in one topic, how many videos i watch on it , i need your video at any cost for my clear understanding and mental satisfaction... Thank you
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
quarantine feels better with learning. thanks for your videos. :)
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Thankyou so much sir , our lecturer teaches us like we’ve already gone through the topic 10 times , he never clears basics and only goes on his lecture saying you already know you although know
If it wasn’t for you I couldn’t have passed
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Teaching is not merely a money earning divice but it is astrugle and life mission. Without ambicues teaching touch souls.thanks.
Thank you. Glad you liked my lectures
You are amazing! You have been saving me throughout my undergraduate years. Thank you so much for everything you do for students all around the world. :)
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of course! Even my American friends love your channel!
@@vrindapatel4091 SAT or GRE ?
I am a university student in America and they way you explain things most of the best professors in my school cant explain it the you do! I would listen to them for hours and not know what they are talking about. But with you, I just cant not understand it!!!
Yesterday my teacher expalined this topic but i am confused many time so just search about electrophoresis and your video is found......thank you for helping to understanding in this topic
Glad to hear that you're getting benefit from my lectures
Thank you very much Sir...these two videos are more than enough to clear our confusion on gel electrophoresis..I wish to know more about Gradient Gel Electrophoresis from you...also watched your lecture on 2D gel electrophoresis...that was also very much interesting...Honestly speaking Sir...you are an Encyclopedia to us...on all these topics on Biology
Thank you so much for appreciating my efforts. You are a loyal subscriber. Keep supporting us.
Nice dress,looking always active and smart! Nice teacher for basic foundation of life science! 🙏
Excellent sir. Ur teaching way is amazing.I was in search of like this way to reach,now my thisrt comes to end to watch this video
Glad to hear that you're getting benefit from my lectures
Thanku sir .... Not only i but every one pray for u... becoz lot of students gain more nd more knowledge through ur video that iz most helpful for our furture .the most thing iz that lot of students that are poor like me can pass their exams easily without any problem
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Ok sir
May I ask you something
I've watched your many videos and my question is how did you learn so much things . I wish I were like you
Thank you so much for appreciating my efforts. I wish I never stop learning
I love your lecturers. You really simplify things
Thank you so much for appreciating my efforts
U r too good sir.....I saw ur all videos and also told my frnds that they watch ur videos😊😊
Thank you. Glad you liked my lectures
Hai sir
After watching ur lecturings sometimes without knowing myself, my eyes got filled with tears, equally am learning and feeling ur lecturings🤗
Thank you my Bhrahma 🙏♥️♥️
Thank you so much for appreciating my efforts. I am honored to see your comment.
Thank you so much sir..for ur efforts... keep it up...n love r style of presenting n discussing...these always save my time..n make my concept 99% clear..
You're welcome. Glad to hear that you're getting benefit from my lectures
Sir i like your videos so much.I like thid one too.....i was unable to understand SDS -PAGE . I search for book too but did not find out.but your video helped mee alot!....pls also make video on detailed applications of modern technquies.....it will be so kuch helpful.🙂🙂🙂
You're welcome. Glad to hear that you're getting benefit from my lectures
Sir this vedio is very helpful for me... I hope you'll make a vedio on 2D Gel electrophoresis soon.. 🙂
Sure
I wish I have the time to watch every single video in your channel
Thank you for your amazing efforts
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U taught way better than my teacher...👍👍👍👍
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It's very helpful to my undergraduate studies. Thank u so much sir
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Sir I had one doubt
In SDS page all the proteins get negative charge from the SDS so that they can easily move towards anode, but in native page there is no charge donator like SDS then how positively charged proteins gets seperated?
Thank you for making my concept on sds-page very clear 😃👍👌
You're welcome. Glad to hear that you're getting benefit from my lectures
Hey! Always thankful for your stuff, incredibly educative. I have a question. Ive been studying 2DGE lately, and my professor always stresses that you need to prepare the sample for the MW based separation with iodoacetamide to remove S-S bonds, but this seems to do the same thing as B-mercaptoethanol, does anyone know why doing both of these things is important, and why Iodoacetamide does not suffice on its own? Thanks!
I am a mpharm pharmacology student.and your videos are helpful.
You're welcome
very well explained lecture.... I must say you are a very brilliant teacher
Great sir, Iam feeling confident after learning subject from u
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Best way to teach
Lots of respect for ur knowledge n teaching skill ❤️
Thank you so much for appreciating my efforts
Respected sir, I request u to upload videos regarding drug discovery technologies and strategies and target validation from genomic and proteomics
I will consider it. Thank you
This was really detailed and helpful... thanks a lot sir!!
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Thank u sir
It is really helpful..
You're welcome
Thank you so much sir, I am extremely thank ful to you, May ALLAH bless you with infinite happiness
Thank you so much for appreciating my efforts
Thank you but can you please state the reason behind using separate gels (stacking and resolving)?
thank you for your valuable lecture sir it helps us a lot
You're welcome
2.5 mAmp current is required in case of semidry transfer. Not in case of SDS-PAGE. Rather after SDS-PAGE the 2.5 mAmp current is used to transfer the proteins from gel to the nitrocellulose or PVDF membrane in case of semidry transfer process only.
Thanks sir..for explaining each and every key points🤗
You're welcome
Wow sir...🔥 awesome explanation
Thank you so much for appreciating my efforts
Thnku sir for your great effort towards us , u make us understand many things , but sir I am really confused to follow which video of shomus biology , means this one or one of your old video , can u plz recommend
New ones
@@shomusbiologyofficial ok sir , thnku a lot
These guy done fresh
Thanks for saving our life❤️
Amazing super cool !! LOVE from Pakistan🤩🤩
Thank you so much for appreciating my efforts
Thankyou so much sir 😊🙏
You're welcome
Thank you dear Sir! Well explained everything 👍
You're welcome. Glad to hear that you're getting benefit from my lectures
Separation take place on the basis of, because all particle have approximately the different charges , and different masses ???? Please tell me
SDS attach to which amino acids of protein??
Guys let's support shomu's biology
Thank you
Sir ur awesome in teaching
Thank you
in sds two types of gel are present one is stacking gel another is resolving gel. it would be much more of help if you have included this as well
I was looking for it actually
Thanks a lot man, you are a great teacher. This is now really clear.
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you verry much ❤️❤️ from Bangladesh
Respect for u
Thank you
very nice explanation sir thank you so much... Sir can I say this video about one-dimensional gel electrophoresis
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Superb 😀 by the way with explanation ur also looking nice sir
Thank you
Thanks bro
Function of SDS is now clear.
You're welcome
Your videos are good but not complete information is provided
Great method. Of explanation
Thank you
Thanks sir ....
You're welcome
Well understood somu sir
Thank you
Also I have one more question that as we use tris glycine buffer as running gel in sds. The glycine enters the gel and help in lining of the proteins before entering the resolving gel. Is it possible to run sds if we don't use glycine.
Nice explained this topic. Sir please suggest some books for research methodology if possible
Coming soon
Thank you so much
Thanks
You're welcome
Love you sir
Thank you
Sir plz as soon as possible make vid on gradient gel
Okay
Great video! I have a question- in one of my experiment, I noticed that a diner protein (44kda) was migrating slower and was found at 50kd. This dimer has positively charged amino acids. Can you please tell me what could be the possible reason for slow migration of this protein?
Is SDS-PAGE is horizontal type of electrophoresis??
Nice teaching sir.
You're welcome
Just awesome..
Thank you
thankyou sir for this video
You're welcome
Thank u sir
How many polypeptide bands detected on gel, if we electrophorised secretory IgA on SDS-PAGE under reduced and denaturing conditions. Sir, plz answer urgently 😔😔😔😔
Best video
Sir, you have not told about the stacking and separating gel difference..
Helpful 😊
You're welcome
What is stacking and separating gels in SDS page ???
Can we separate RNA by Agaros gel also?
And if we have mixture of DNA snd mixture of RNA in gel, who will being faster? DNA or RNA
Or we not compirsion DNA and RNA togather?
Just DNA size it self and RNA itself?
Can you please make videos on molecular biology experiments? Such as DNA isolation, protein estimation, protein purification
Okay
I have one question what is denaturing polyacrylamide gel electrophoresis and how is this different from native page or SDS page?
In native gel, we don't denatured the protein. It moves as it is in structure and in denatured gel, we denatured proteins and then allow it to migrate in gel
Sir,why we need to separate the protein...clinical significance???
Great sir....
You're welcome
Thanku sir
Thank you so much
You're welcome
Sir really really thank you so much 😊
Sir mujhe 1 Question puchhna h
SDS-PAGE m do Ph Q lete h Resolving Gel & Stacking Gel k lie ?
Please sir please please reply with d answer.
I have to answer this
Please sir
Sir where Resolving gel and stacking gel?
How much of voltage is sufficient to run an SDS PAGE to get a protein band of molecular weight 27kDa
Why we get four bands for BSA in SDS PAGE? If you have an proper explanation then please help me?
Tus vídeos son geniales puedo entender a la perfección sólo que por favor podrías agregarle subtítulos en español n.n!
learn English its a global language
Well explained
Thank you
Sir plz refer any molecular book related to techniques e.g electropheris, sds, fish qrt pcr hybridization
Bloating pcr etc plzzzzzzzzzz
told the author name of that book your lecture is good thanx keep it up
Thank you. Glad you liked my lectures
Nyc sir very helpful 😊
You're welcome
Please make a video on immune electrophoresis
Okay
Thank you❤️❤️
You're welcome
@@shomusbiologyofficial ❤️❤️❤️
Which buffer is used?
SDS-PAGE is also a discontinuous gel system made up of two different pH gels called stacking gel of pH 6.8 and separating gel of pH 8.8 which also differ in its pore size. You have not mentioned this important point
Thank you for mentioning
@@shomusbiologyofficial Thank you sir. Also thank you for such easy and well explained videos.
Sir, which amino acid interfere with the SDS page?
Sir can u plxx....arrange a class on ultra centrifugation
Coming soon
Sir my test coming soon ....can u arrange plzz before 1june
Sir but plzz it should be in urdu language and on white board ...i mean as u given ur SDS topic on white board
Why SDS give 2 band in antibody separation?? Plz ans...
Thanku sir s much
You're welcome
Can you please discuss on the mathematical problems based on SDS Page and ESI-MS? Like the ones asking for molecular weight of the protein?