this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.
This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.
Hello! This video really saved me! Detailed and exhaustive. I was studying this technique for an exam and didn't understand it really...now it's all clear. Thanks! 😊
Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam: ua-cam.com/video/On_ZotdZexI/v-deo.html
OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...
Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)
Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!
OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!
Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.
I really don't understand why the stacking gel concentrates the proteins. If it is still a gel the proteins will be administered as they are pulled from the solution so they would enter the stacking gel separated. What is stopping the proteins from doing this? And how are the loose glycine molecules related. It doesn't seem like they have anything to do with the proteins, they are just there like chloride.
How can we distinguish the different proteins and their polymorphisms from each other after the separation. If, say our sample is cattle milk and we are interested in casein and all it's various polymorphism alpha, beta, kappa etc. Are we going to use the same or different markers to identify the required proteins.
Hi. at the end of the video, you mention that the proteins are not applied to the stacking gel at the same time, but they do so in the separating gel. What does this statement mean? what is meant by being applied to the gel at the same time?
I have few questions. 1. Why do we have 8.3 pH in electrodes? What is the significance? 2. Is the gel already made or prepared during the experiment? Thanks in advance.
Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.
They only thing I didn’t get is why do we need the migration buffer (i.e the chloride and Glycine) in the gel if the proteins will be separated from them and travel to the negative side of the gel eventually?
Hi, I want to stady the expression of a big protein wich mesure 358kda using western blot. How can I prepare a separating gel with a small gradient to visualize this big protein?
Thank you this helps a lot! the article i'm reading talks about 3 different gels running gel, gradient gel and stacking gel, In this video we are shown separating gel, which one of the two (running or gradient gels) would the separating gel be equivalent to?
I wanna ask a question it wasn't mentioned in the video What does it mean a monocharged protein? What will be the answer if the question is : Gel electrophoresis separates the monocharged proteins? What are monocharged proteins?
Why can't my professor just memorise this video and rap it during the lab. Very useful!
Haha thank you ... maybe you can recommend it to your colleagues ;)
throw that ish on Rap Chat yo, that would be FIRE!
your professor probs done thousands of 'gels' while doing his first research work. I m assuming he's just not interested any more:-))
this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.
Hehe
I just have to say it was the best explantion i've ever heard about anything! Thank you, it answered all of my questions....:)
This helped me a lot before even doing my practicals. Thank you
This is the best video I found on the internet about SDS PAGE. Thank you so much for all the details.
This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.
Hello!
This video really saved me!
Detailed and exhaustive.
I was studying this technique for an exam and didn't understand it really...now it's all clear.
Thanks! 😊
This is the best video I have seen on the working principle of SDS PAGE. Thank you
More detailed than other videos on SDS-PAGE.
yup, stay tuned for more videos :)
thanks for the explanation, the best thing is that you dedicate time to explain the principle of the gel, its the best explanation i´ve seen so far :D
Perfect ! I spend time looking for those informtion on internet and you give them (and a lot more) very clearly, good job !
Hello, thank you for your comment ... happy you got what you need from the video :)
Biomedical and Biological Sciences yes i actually have an exam about all those technics soon and you saved my life haha !
Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam:
ua-cam.com/video/On_ZotdZexI/v-deo.html
I had watched many videos to understand this topic, but this video is definitely the best! Thank you so much!
I finally undertood Electrophoresis!!!! THANK YOU! Greetings from Argentina ♥
Hei, Glade you liked the video ... stay tuned :)
Mam please keep uploading such videos so that we can have clear concept about each technique ur way of teaching is great😄
Thank you so much for the explanation, especially for the stacking gel's part! I found it very clear.
Really really really good explanation...I was searching for this type of detailed explanation in whole of UA-cam.....
This is the most helpful video i have ever watched about sds page. Now i understand much more about this! Thank you so much
you have my thanks madam for saving me from mid exam disaster. Thank you so much
OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...
Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)
thank you so much for the hard work you put into this video! it is very much appreciated :) your video has helped me a lot in the lab today!
For SDS, I just watch this... Thank you for this indepth information mam🙏🙏
I wish I will have a teacher like you in the future. terrific and detailed explanation
I am too pleased to watch your videos, these are very clear concept growing video. Thank you very much keep it up.
Thank you for your comment ... I will ... Stay tuned :)
And somebody has been messing with my brain all this while. Anytime I see you in person, I will give you a bottle of win.
Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!
Awesomely explained.. Thanks very much mam. Lots of love and respect from Bangladesh
OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!
WOOW .. thank you for the nice comment ... stay tuned, you might find everything you are searching for in this channel :)
you are so amazing, keep going like this!! this is THE ONLY VIDEO i found explained well
Beautiful explanation! Love this ❤
Thank you so much video is very helpful to understand better SDS page
True
Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.
Very well explained! Do you have any sources for this? I would like to refer to it in my masters.
thank you so much, the explanation was very descriptive and reasonably good.
i find it very useful thankyou so much for make that kind of videos
Congrats! Very helpful video, I understood everything I needed for my lab. Thank you! (the explanation is very good and well constructed)
Explanation is fantastic but it will very well if you explain how stacking and separating gel is prepared...
The most helpful video ever! Thank you so much!
Very nice explanation of a difficult concept of SDS PAGE 👍👍👍👍
extremely helpful video for beginners
Oh my god so useful video... you save my grade
I really don't understand why the stacking gel concentrates the proteins. If it is still a gel the proteins will be administered as they are pulled from the solution so they would enter the stacking gel separated. What is stopping the proteins from doing this? And how are the loose glycine molecules related. It doesn't seem like they have anything to do with the proteins, they are just there like chloride.
Really such a amazing explanation 😘🤩
How can we distinguish the different proteins and their polymorphisms from each other after the separation.
If, say our sample is cattle milk and we are interested in casein and all it's various polymorphism alpha, beta, kappa etc. Are we going to use the same or different markers to identify the required proteins.
This video was very useful. Thank you ma'am
Thank you very much for the video. You're a good teacher. 😊
Thanks Dr. what are the advantages and disadvantages of SDS page compparesmd to the native Page
Superb explaination
This is a really great explanation of d topic
Outstanding explanation ❤
Thankyou so much for the explanation. I loved it 😊😊. Good work.
Superb video!! Insanely helpful! Thank you so much!!
Thank u very much u r wonderful keep making these videos I learnt a lot and many things become clear
Nicely done yet again.
Clear and to the point. Thank you
Your videos are excellent!
Hi. at the end of the video, you mention that the proteins are not applied to the stacking gel at the same time, but they do so in the separating gel. What does this statement mean? what is meant by being applied to the gel at the same time?
Excellent Explanation 👍👏👏👏
Keep it up 👍
This was very helpful! Thank you!
Very helpful keep it up.
So clear ,good job 👍
Why and how proteins have been aligned in the stacking gel? Thank you.
Very well explained........thank you so much mam
I have few questions.
1. Why do we have 8.3 pH in electrodes? What is the significance?
2. Is the gel already made or prepared during the experiment?
Thanks in advance.
the same to you
Thank you mam .for giving a good visual memory of SDS.page .
Awesome teaching. Thank you so much.
welcome ... Thank you for the comment :)
This is so useful. Life Saver!
Love the video! Very informative. Thank you so much
Thnx sooooo much.
Very clear and important
Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.
Just amazing....y der are no more videos posted? It will b great if u post about centrifuge
Very well put together. loved this video Thank you :)
Finally I understood 🤗🤗🤗 thank uuuuuuu
thanku for clearing my doubts about this techniques
Solid video! Thanks a million times!
Subbed!
I finally understood it! Thanks!
thank you for the video. can you make a video of how to read the protein electrophoresis result?
Perfect .👍 simply wht i was actually searching for🙌
So helpful, thanks so much!
indeed best video for beginners.
thank you mam....for such a well explained lecture.
Best WB video ever!
ty, this video helpme in the exam
thank you very informative ! keep going from Saudi arabia Salam
Very informative. Thank you😍
very good explanation. Thank u!
very good explained !
They only thing I didn’t get is why do we need the migration buffer (i.e the chloride and Glycine) in the gel if the proteins will be separated from them and travel to the negative side of the gel eventually?
Thank you very much!!! I'm just from the lab where i did all this without understanding anything!! hahahahaha
HAHAHA this is normal ... Sometimes we do things without going into the details ... stay tuned in the channel so you can see all the new videos :)
Very good explanation ...really impressed..please make biochemistry molecular biology cell biology related videos to help us..thank you
Amazing' ❤️❤️
Thanks ma'am❤️
Such a perfect video
Hi, I want to stady the expression of a big protein wich mesure 358kda using western blot. How can I prepare a separating gel with a small gradient to visualize this big protein?
Loved the video..
But why we don't use two gels in agarose gel electrophoresis like PAGE
Because agarose is mostly used for purification of DNA/RNA
Thank you this helps a lot! the article i'm reading talks about 3 different gels running gel, gradient gel and stacking gel, In this video we are shown separating gel, which one of the two (running or gradient gels) would the separating gel be equivalent to?
Thank you for the really great explanation. I still don't quite understand why the stacking gel is needed in the first place. Can someone please help?
Amazingly explained, thank you!!
I wanna ask a question it wasn't mentioned in the video
What does it mean a monocharged protein?
What will be the answer if the question is : Gel electrophoresis separates the monocharged proteins?
What are monocharged proteins?
Pls can u answer this question about material that you say (protein )
Why we use half the grain of wheat without an embryo?
great teaching.. thanks a lot...
Thank you for your comment .. welcome to my channel :)
Mam your videos are very good. It is very easy to understand this. Mam can you please make a video on immunoelectrophoresis. Thank you so much 😊