Total Plate Count (Total Aerobic Bacterial Count)_A Complete Procedure (BAM, Ch-3)

Total Plate Count (Total Aerobic Bacterial Count) is a very important Microbiological testing parameter for Food, Feed, Water, Environmental and other samples analysis. This video represents a detailed procedure for the enumeration of total bacteria in a sample.
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[@dasarisailaja-uv3lq]

Very good explanation thanks a lot

[@afolabipeter7073]

Am blessed to have watch this❤

[@MicroChemsExperiments]

Thank you

[@gousiagulzar7002]

Your videos are saviour for people like me who are not from pure microbiology field 🥹

[@MicroChemsExperiments]

Thank you so much.

[@SachinYadav-sk3fh]

Wow very useful video
Thank you for your all the videos.
Love you ❤️

[@MicroChemsExperiments]

Thanks

[@sulemankhanvirologist5015]

"Excellent explanation."

[@mukkerasreeya4457]

Explained so clearly

[@MicroChemsExperiments]

Glad to know that it was helpful

[@mattiti2919]

What is the ISO (standard method) for this? We might need this for our research. Thank you

[@KhalidSaleem-un8hd]

Wow it's Amazing and helpful❤

[@MicroChemsExperiments]

Thank you

[@chigozieofoedu1731]

Nice one. Thanks

[@hamidr_nazari]

Love you 😀🙃🙂😁😃 its so usefull

[@MicroChemsExperiments]

Thanks

[@rakibhassan2451]

Your video's always awesome 😘😘😘

[@MicroChemsExperiments]

Thank you

[@ashapatil992]

Very nice procedure thank you

[@MicroChemsExperiments]

Thank you

[@officiallighthouse]

Excellent, very clear and to the point presentation.

[@MicroChemsExperiments]

Glad you liked. Thanks

[@mahnoorsohail8479]

best video 😍

[@setiawanalwisius-tu6vx]

Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?

[@MicroChemsExperiments]

There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.

[@jogaramesh2429]

I would like to know what justification has to be provided to the USFDA for more bacterial count to the first 4 plates and reject them. Reason for fixing last dilutions to the fda

[@asmeretkidane4269]

very nice presentation continue with clear

[@FirehiwotDaselagn]

nice peresentation

[@MicroChemsExperiments]

Thanks

[@balurajnadeesh2788]

Very useful vdo

[@MicroChemsExperiments]

Thank you

[@IzzyRoxy]

Very clear explanation, really helped me alot thankssss 🥹💖

[@paleshwongshrestha2798]

Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder

[@MicroChemsExperiments]

Noted

[@biannekaulf9221]

Wow, thank you very much for sharing this.

[@MicroChemsExperiments]

You are welcome

[@zevanoc.sibarani8295]

Can you tell me what is the standard method you use to do this procedure? Thank you 🙏

[@habibakhan3555]

Fda bam chapter 3 for Total aerobic count

[@elinagwimile6136]

FDA BAM - CHAPTER 3

[@GMCLABORATORY]

Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?

[@efeodjegba4215]

this is amazing thank you

[@chauhanasmita4651]

It's really helpful thank you

[@MicroChemsExperiments]

Thank you

[@ervingopo5734]

Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?

[@MicroChemsExperiments]

To avoid condensation of water vapour on the lid of the dish because the media is still hot

[@microbegist7918]

I find this content helpful as a student doing her project.

[@deedee4527]

Thank you so much, well explained.

[@MicroChemsExperiments]

Thank you

[@abhijitshukla437]

Which microbes present in these PCA plates please tell me name of microbes

[@renutailorshorts8155]

Hello ma'am, this is same for anaerobic bacteria also

[@fathimasidhiky4412]

very well explained

[@MicroChemsExperiments]

Thank you

[@SIMRANPAL-s8m]

can you help me with what TPC count should be considered in acceptable range
for frozen fruits and vegetables?

[@mdsajibhossain747]

Same methods can we use for Drinking water or juice analysis?
One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water.
Thank you very much.

[@MicroChemsExperiments]

Same method is for Drinking water or juice analysis.
We will make video for yeast and Molds and Magnesium analysis

[@bashettys]

When we can use the words Absent and Less than 10 if there is no growth...and if there is no growth can we mention Zero or not...

[@abdallahadam9734]

I loved it

[@MicroChemsExperiments]

Thanks

[@renutailorshorts8155]

Please upload video for disinfectation using chlorine or which is suitable

[@HK-Media1]

Nice

[@MicroChemsExperiments]

Thank you

[@nankwopmarkus4884]

Why using phosphate buffer?
Can one use distilled water instead?

[@asmeretkidane4269]

how many sample is done in the same time ?

[@adzansaban3731]

Sir, do you turn on the blower when working in the laminar?

[@TOM-l6b3f]

Same doubt ..do you know the answer?

[@purushotamgorasiya7109]

Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?

[@MicroChemsExperiments]

Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate

[@elsabeteyohannes6213]

Thank you for your excellent explanation

[@ervingopo5734]

Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.

[@MicroChemsExperiments]

To avoid condensation of water vapour on the lid of the dish because the media is still hot

[@999_finesse]

I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3
Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml
The accepted count is

[@MicroChemsExperiments]

There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit

[@mani-ls7kj]

Please share the determination of ammonium acetate assay method

[@MicroChemsExperiments]

Noted

[@rjedey658]

Mam can I use this technique for khoaya,Chenna or any milk based sweets?

[@MicroChemsExperiments]

Yes you can

[@sashikalawanasinghe1021]

Can this procedure use to butter sample?

[@MicroChemsExperiments]

No

[@sashikalawanasinghe1021]

What is the procedure used to butter sample ? 😢

[@dessienatalia1193]

Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?

[@MicroChemsExperiments]

Yes

[@meenageeths9730]

Very easy planas

[@MicroChemsExperiments]

Thanks

[@gousiagulzar7002]

Can we use peptone instead of buffer you mentioned

[@MicroChemsExperiments]

You can use peptone salt solution

[@neethuaneethua941]

I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.

[@MicroChemsExperiments]

Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks

[@neethuaneethua941]

​@@MicroChemsExperimentsthank you

[@neethuaneethua941]

Can you please upload a video about enrerobacteriacea (IS 17112 part ll

[@MicroChemsExperiments]

@@neethuaneethua941 noted

[@neethuaneethua941]

I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.

[@yosam9393]

sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?

[@MicroChemsExperiments]

Will make leter

[@gargireghunath2114]

Is this method used for testing shrimp?

[@MicroChemsExperiments]

Yes, applicable

[@khushiyadav6723]

Does the same method used in industries?

[@MicroChemsExperiments]

Yes. You can use

[@saurabhdeshmukh2605]

Of which sample you had calculated this cfu

[@MicroChemsExperiments]

Soyabean meal

[@venkybunny1554]

would you please make a video on the hanna ph meter

[@MicroChemsExperiments]

Already made. Link: ua-cam.com/video/PRxJgmzd5jE/v-deo.html

[@MrLongKR]

hi. may i know the reference for this procedure?

[@MicroChemsExperiments]

BAM, Chapter 3

[@ynen_405]

Can i use Nutrient agar and peptone water in cookies? Is it acceptable?

[@MicroChemsExperiments]

You can use and will get results

[@zarqaarshad411]

Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths

[@MicroChemsExperiments]

Noted

[@manjiripatwardhan336]

Can you please make video on MPN method for detection of Esheresia coli.

[@MicroChemsExperiments]

Noted

[@karthiyayanikrishnan5675]

How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?

[@MicroChemsExperiments]

Make report as

[@yen4310]

What if my acceptable counts is in the first dilution, will the formula be C1/1xd?

[@MicroChemsExperiments]

Yes. Please share your final results here

[@callistaemeali5052]

Can I use plate count agar and peptone water? Is that acceptable?

[@MicroChemsExperiments]

Yes. You can use.

[@akramasgari]

Best 👌

[@kotaspecial9047]

Can you guide me how to test tri basic lead sulphate testing method

[@MicroChemsExperiments]

Will upload soon

[@karntokaali9778]

Can I replaced by nutrient agar media?????

[@MicroChemsExperiments]

You can but without any reference

[@juharAmeumare]

Adrenergic receptors activators

[@juharAmeumare]

Adrenergic receptors activators

[@vikashganvit3548]

Ma'am how to do antimicrobial activity of polymer films?

[@MicroChemsExperiments]

Same as shown in the video

[@vikashganvit3548]

@@MicroChemsExperiments polymeric film is solid

[@MicroChemsExperiments]

@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole

[@DESIVOCALTV]

Why we make two/more petri plates of same dilution ?

[@MicroChemsExperiments]

There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates

[@TOM-l6b3f]

Why can't we take the average of trial 1 and 2 and consider it as Cfu ? Do any one know??

[@MicroChemsExperiments]

You can just average of two trials

[@rutujajadhav9446]

Which medium should be used total viable count of milk

[@MicroChemsExperiments]

Plate Count Agar

[@rajuahmmed1257]

Could you please upload the quantification video of beta carotenoid from carrot sample by UHPLC?

[@MicroChemsExperiments]

Noted.

[@bulakochebo7636]

please whose protocol is it?

[@MicroChemsExperiments]

Reference Method is already mentioned. Please check again

[@suzaava3691]

In the formula, how did you get 1 and 0.1 please.
Thank you

[@MicroChemsExperiments]

The formula is given by BAM, CHAPTER 3

[@safeedhasaidalavi9786]

Can you pls tell the limit for total bacterial count in the case of biscuits

[@MicroChemsExperiments]

100CFU/g

[@sonal3124]

Which method use? Pls send the method name

[@MicroChemsExperiments]

Reference is given in this video

[@ankitsaxena8738]

Ky ap ye video hindi me nhi bna skte hain aur 🙏❤️

[@Madhuu151]

If there is a video that calculates the histamine level in a fish sample using the Elisa method, can you please provide the link?

[@MicroChemsExperiments]

No video was made on this test yet.

[@mughuutubeshorts3209]

Can I analyze the total bacterial count in sewage sludge by this method?​@@MicroChemsExperiments

[@degontoislam925]

could you please share the reference for this work, thank you

[@MicroChemsExperiments]

BAM chapter 3

[@degontoislam925]

@@MicroChemsExperiments thank you

[@anbarulhaque4720]

3.21 cfu/g is my reading
.: Then how many colonies are there

[@MicroChemsExperiments]

There are about 3 colonies in 1g of your sample.

[@anbarulhaque4720]

How to convert cfu/g to cfu/ml

[@MicroChemsExperiments]

These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample

[@megharaghuwanshi4102]

How to perform B.et test

[@MicroChemsExperiments]

Please write the full name of the test

[@somnathchanda1250]

Use natural accent

[@MicroChemsExperiments]

Ok. Will try

[@dr.shashankbaghel]

thanks but you should avoid fake accent

[@anbarulhaque4720]

3.21 cfu/g is my reading
.: Then how many colonies are there

[@MicroChemsExperiments]

There are about 3 colonies in 1g of your sample.